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alcohol dehydrogenase 5 of Helicoverpa armigera interacts with the CYP6B6 promoter in response to 2-tridecanone

Insect Science 2020年第5期27卷 1053-1066页

作者： Jie Zhao Qian Wei Xin-Rong Gu Su-Wei Ren Xiao-Ning Liu Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Sciences and TechnologyXinjiang UniversityUrumqiXinjiangChina Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Resources Utilization College of AgricultureShihezi UniversityShiheziXinjiangChina

alcohol dehydrogenase 5(ADH5)is a member of medium-chain dehydro-genase/reductase family and takes part in cellular formaldehyde and S-nitrosoglutathione metabolic network.2-tridecanone(2-TD)is a toxic compound in many Solanaceae crops to defend against a variety of herbivory *** the broader context of insect development and pest control strategies,this study investigates how a new ADHS from Helicoverpa armigera(HaADH5)regulates the expression of CYP6B6,a gene involved in molting and metamorphosis,in response to 2-TD *** of the HaADH5 complementary DNA sequence revealed that its 1002 bp open reading frame encodes 334 amino acids with a predicted molecular weight of 36.5 ***5 protein was purified in the Es-cherichia coli Transetta(pET32a-HaADH5)strain using a prokaryotic expression *** ability of HaADH5 protein to interact with the 2-TD responsive region within the promoter of CYP6B6 was confirmed by an in vitro electrophoretic mobility shift assay and transcription activity validation in ***,the expression levels of both HaADH5 and CYP6B6 were found to be significantly decreased in the midgut of 6th instar larvae after 48 h of treatment with 10 mg/g 2-TD artificial *** results indicate that upon 2-TD treatment of cotton bollworm,HaADHS regulates the expression of CYP6B6 by interacting with its *** HaADH5 regulation of CYP6B6 expression may con-tribute to the larval xenobiotic detoxification,molting and metamorphosis,HaADH5 is *** target for controlling the growth and development of cotton bollworm.

关键词： alcohol dehydrogenase DNA-protein interaction Helicoverpa armigera regulatory factor 2-tridecanone

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alcohol dehydrogenase Activity in Cultured Cells from Different Tobacco (Nicotiana tabacum L.) Varieties

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Wuhan University Journal of Natural Sciences 1999年第4期4卷 495-497页

作者： ZHENG Ling, YANG Yue sheng ,ZHENG Ying dong College of Biotechnology, South China Agricultural University, Guangzhou 510642,China South China Agricultural University College of Biotechnology Guanzhou China (GRID:grid.20561.30)

The activity of alcohol dehydrogenase (ADH) in cultured cells of various tobacco was determined. It was found that significant differences existed in cells of different varieties cultured under normal conditions and as well after treated with exogenous ethanol. The ADH activity had positive relation with the ability of the cells to catabolize exogenous ethanol, indicating that the main function of the ADH in tobacco cells was in the direction of converting ethanol to acetaldehyde.

关键词： alcohol dehydrogenase suspension cultured cells tobacco

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Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

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Journal of Integrative Agriculture 2017年第8期16卷 1742-1750页

作者： QIN Gai-hua QI Xiao-xiao QI Yong-jie GAO Zheng-hui YI Xing-kai PAN Hai-fa XU Yi-liu Horticultural Research Institute Anhui Academy of Agricultural SciencesHefei 230031P.R.China Anhui Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crop Hefei 230031P.R.China College of Agriculture Medicine and HealthAnhui Radio and Television UniversityHefei 230022P.R.China

alcohol dehydrogenase （ADH） catalyzes the interconversion of aldehydes and their corresponding alcohols, and is a key enzyme in volatile ester biosynthesis. However, little is known regarding ADH and ADH encoding genes （ADHs） in pear. We identified 8 ADHs in the pear＇s genome （PbrADHs） by multiple sequences alignment. The PbrADHs were highly ho- mologous in their coding regions, while were diversiform in structure. 9 introns were predicted in PbrADH3-PbrADH8, while 8 introns, generated through exon fusion and intron loss, were predicted in PbrADH1 and PbrADH2. To study the genetic regulation underlying aroma biogenesis in pear fruit, we determined the PbrADH transcripts, ADH activities and volatile contents of fruits during ripening stage for Nanguoli and Dangshansuli, two cultivars having different aroma characteristics. ADH activity was strongly associated with the transcription of ADH~ in the two cultivars during fruit ripening stage. The higher ester content paralleling to a higher ADH activity was detected in Nanguoli than in Dangshansuli, so it is induced that the lower ester content in Dangshansuli fruit may be the result of weak ADH activity. The present study revealed that total ADH activity and volatile ester production correlated with increased PbrADHstranscript levels. PbrADH6 may contribute to ADH activity catalyzing aldehyde reduction and ester formation in pear fruit.

关键词： alcohol dehydrogenase ester volatile gene expression pear

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alcohol dehydrogenase coexisted solid-state electrochemiluminescence biosensor for detection of p53 gene

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Journal of Southeast University(English Edition) 2013年第2期29卷 145-151页

作者：王晓英王晓宁张相依陈奋天朱柯蕙杨立刚唐萌东南大学环境医学工程教育部重点实验室南京210009 西安交通大学第一附属医院血液科西安710061

An alcohol dehydrogenase （ADH）-coexisted solidstate electrochemiluminescence （ECL） biosensor for sensitive detection of the p53 gene was developed. The electrode modified by multiwalled carbon nanotubes, Ru（bpy）]2＋3 and polypyrrole （ MWNTs-Ru （bpy） ]2＋3 -PPy ） was prepared to adsorb the ssDNA by electrostatic interactions. Then, the ssDNA recognized the gold nanoparticles （AuNPs）-labeled p53 gene and produced the AuNPs-dsDNA electrode with the AuNPs layer. The AuNPs layer adsorbed the ADH molecules for producing the ECL signal. Thus, the biosensor was based on coupling enzyme substrate reaction with solid-state ECL detection, and it displayed good sensitivity and specificity. The detection limit of the wild type p53 sequence （wtp53） is as low as 0. 1 pmol/L and the discrimination is up to 57. 1% between the wtp53 and the muted type p53 sequence （mtp53）. The amenability of this method to the analyses of p53 from normal and cancer cell lysates is demonstrated. The signal of wtp53 in the MGC-803 gastric cancer cell lysates turns out to be about 61.8% that of the wtp53 in the GES-1 normal gastric mucosal cell lysates, and the concentration of the wtp53 is found to decrease about 59 times. The method is highly complementary to enzyme-linked immunosorbent assay （ELISA）, and it holds promise for the diagnosis and management of cancer.

关键词： MWNTs-Ru ( bpy )2+3 composite solid-stateelectrochemiluminescence alcohol dehydrogenase wild typep53 sequence muted type p53 sequence cell lysates

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Achieving Enzyme Stability Using a Simple Fabrication Procedure： The alcohol dehydrogenase Example

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Journal of Pharmacy and Pharmacology 2018年第2期6卷 188-196页

作者： I. Ribau E. Fortunato Departamento de Ciencias dos materiais Faculdade de Ciencias and Tecnologia Universidade Nova de Lisboa Campus de Caparica Caparica 2829-516 Portugal

The use of screen-printing biosensors has been updated in this article as a tool to analyze the electron transfer process involving redox proteins or enzymes. The aim of this research was to fabricate a simple apparatus which allowed the use of the enzymes （in the solid state） to maintain their stability. To prove this concept an enzyme in the solid state was mixed with the carbon ink and this mixture was used to print the working electrode. We choose as proving the alcohol dehydrogenase. The first reason is because it metabolizes the alcohol, which can be present in biological samples of blood, saliva and urine and also in the beverage; the second is that this enzyme is still a challenge to electrochemistry due to having lower stability in sensors. The results show that in this device the enzyme was active and stable during all the experiments and in the experimental conditions that could catalyze the ethanol to acetaldehyde. These devices have the advantage of being disposable, cheap and are easy to fabricate. And also, they do not need expensive tools to be fabricated, they only need 2μL of electrolyte or sample, and they need lower amounts of enzyme to permit electrochemical studies.

关键词： Paper biosensor disposable alcohol dehydrogenase multiple use.

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Association of alcohol dehydrogenase and aldehyde dehydrogenase 2 genetic polymorphisms with serum lipid profile: meta-analysis of mendelian randomisation studies

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South China Journal of Cardiology 2014年第4期15卷 238-250,256页

作者： GAO Jia-jia JIN Dong-mei WEN Zhu-zhi IP Chi-kin WANG Jing-feng GENG Deng-feng Department of Cardiology Sun Yat-sen Memorial Hospital Sun Yat-sen University Department of Rehabilitation Medicine Sun Yat-sen Memorial Hospital Sun Yat-sen University

Background alcohol dehydrogenase （ADH） and aldehyde dehydrogenase 2 （ALDH2） are the key en- zymes for alcohol metabolism. Several genetic studies have investigated the association between ADH and ALDH2 genetic polymorphisms and serum lipid profile （SLP）, however, the results were inconsistent. Methods Fourteen articles involving 27,917 participants were included in this meta-analysis. Weighted mean difference （WMD） and 95% confidence intervals （CIs） were presented using random effects model. Publication bias was evaluated by funnel plot and Begg＇s test. In addition, to further explore the heterogeneity, subgroup analysis were performed. Results Overall, there was no association between ADH genetic polymorphisms and SLP with no regard for drinking status. However, compared with ALDH2 wild homozygous genotype, ALDH2 mutant genotypes were associated with significant decrease in serum high density lipoprotein cholesterol （HDL- C）（WMD -1.80 mg/dL, 95% CI -1.88 to -1.72, P 〈 0.001） and total cholesterol （TC） levels （WMD -1.]0 mg/dL, 95% CI -1.59 to -0.62, P 〈 0.001）, and significant increase in serum low density lipoprotein choles- terol （LDL-C） level （WMD 0.30 mg/dL, 95% CI 0.18 to 0.43, P 〈 0.001）. Although there was no significant difference in serum triglyceride level in the overall population, subgroup analysis revealed that compared with ALDH2 ＊ 1 wild homozygote, ALDH2 ＊ 2 allele displayed a significant difference in serum triglyceride level between the female and male （female： WMD 1.69 mg/dl, 95% CI 1.08 to 2.30, P 〈 0.001; male： WMD -6.42 mg/dL, 95% CI -12.15 to -0.68, P = 0.028）. Conclusion ADH genetic polymorphism has no association with SLP, regardless of sex category and drinking status. ALDH2 genetic polymorphism has slight association with HDL-C, LDL-C and TC levels and sex-specific association with serum triglyceride level. Whether or not the association between ADH2 genetic polymorphisms and SLP is resulted from alcohol con-sumption needs further investi

关键词： alcohol dehydrogenase aldehyde dehydrogenase serum lipids meta-analysis

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Membrane-bound alcohol dehydrogenase Enzyme is Responsible for Enantioselective Oxidation of 1,2-Proponediol to D-(-) Lactic Acid by Gluconobacter Oxydans M5

Membrane-bound Alcohol Dehydrogenase Enzyme is Responsible f...

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The 12th Asian Pacific Confederation of Chemical Engineering Congress

作者： Liujing Wei Xuepeng Yang Guodong Wei Jinping Lin Dongzhi Wei State Key Lab of Bioreactor Engineering,New World Institute of Biotechnology,East China University of Science and Technology,shanghai,China

The alcohol dehydrogenase(ADH)of Gluconobacte Oxydans M5 was isolated from the mem- brane fraction,and *** dehydrogenase consist two subunits with molecular weights of a- bout 80 and 50 *** larger subunit of this enzy... 详细信息

关键词： gluconobacter alcohol dehydrogenase 1,2-proponediol D-(-) lactic acid

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Positive effect of ethanol-induced Lactococcus lactis on alcohol metabolism in mice

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Food Science and Human Wellness 2022年第5期11卷 1183-1190页

作者： Sisi Chen Shimin Jia Keke Suo Qiaozhen Kang Limin Hao Laizheng Lu Xin Liu Jinyong Huang Jike Lu School of Life Sciences Zhengzhou UniversityZhengzhou 450001China Physical Education College Zhengzhou UniversityZhengzhou 450001China The Quartermaster Research Institute of Engineering and Technology Academy of Military Sciences PLA ChinaBeijing 100010China Zhengzhou Mindtek Biotechnology Co. Ltd.Zhengzhou 450001China School of Agricultural Sciences Zhengzhou University 450001China

It is well known that exposure to environmental stresses could enhance the adaptability of bacteria and up-regulate the expression of a variety of oxidative stress-related genes and antioxidant *** is unclear whether the adaptability of microorganisms formed naturally in special environments could transfer to other *** study aimed to evaluate the effects of untreated and ethanol-induced Lactococcus lactis intracellular extracts(U-IE and E-IE)on alcohol metabolism in *** positive effects of E-IE on alcohol metabolism in mice were revealed by the enhanced latency of loss of righting reflex(LORR),the reduced duration of LORR,the decrease of blood alcohol concentration,as well as the elevation of alcohol dehydrogenase(ADH)activities in the stomach and liver ***,the potential benefits of E-IE on the liver were evaluated by biochemical parameters including the activities of serum transaminase,the levels of antioxidant enzymes,and the pathological changes of liver *** present work put forward a new point that appropriate ethanol stress could enhance the intracellular ADH activity of ***,and its intracellular extracts could continue to enhance alcohol metabolism in mice.

关键词： Ethanol stress Intracellular extracts alcohol dehydrogenase alcohol metabolism

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Meta-analysis reveals up-regulation of cholesterol processes in non-alcoholic and down-regulation in alcoholic fatty liver disease

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World Journal of Hepatology 2017年第8期9卷 443-454页

作者： Wasco Wruck James Adjaye Medical Faculty Institute for Stem Cell Research and Regenerative MedicineHeinrich Heine University40225 DusseldorfGermany

AIM To compare transcriptomes of non-alcoholic fatty liver disease(NAFLD) and alcoholic liver disease(ALD) in a meta-analysis of liver *** Employing transcriptome data from patient liver biopsies retrieved from several public repositories we performed a meta-analysis comparing ALD and *** We observed predominating commonalities at the transcriptome level between ALD and NAFLD,most prominently numerous down-regulated metabolic pathways and cytochrome-related pathways and a few up-regulated pathways which include ECM-receptor interaction,phagosome and *** some pathways were regulated in opposite directions in ALD and NAFLD,for example,glycolysis was down-regulated in ALD and up-regulated in ***,we found rate-limiting genes such as HMGCR,SQLE and CYP7A1 which are associated with cholesterol processes adversely regulated between ALD(down-regulated) and NAFLD(up-regulated).We propose that similar phenotypes in both diseases may be due to a lower level of the enzyme CYP7A1 compared to the cholesterol synthesis enzymes HMGCR and ***,we provide a compendium of comparative KEGG pathways regulation in ALD and *** Our finding of adversely regulated cholesterol processes in ALD and NAFLD draws the focus to regulation of cholesterol secretion into ***,it will be interesting to further investigate CYP7A1-mediated cholesterol secretion into bile-also as possible drug *** list of potential novel biomarkers may assist differential diagnosis of ALD and NAFLD.

关键词： Non-alcoholic fatty liver disease alcoholic liver disease cholesterol Bile alcohol dehydrogenase CYP7A1

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Genotype of ethanol metabolizing enzyme genes by oligonucleotide microarray in alcoholic liver disease in Chinese people

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Chinese Medical Journal 2002年第7期115卷 1085-1087,156+155页

作者：虞朝辉厉有名陈卫星乐敏浙江大学医学院第一附属医院杭州310003

OBJECTIVE: To explore the relationship between genetic polymorphisms of the ethanol metabolizing enzymes and the occurrence of alcoholic liver disease (ALD). METHODS: Sixty-five healthy male controls and 165 alcoholisms (including 122 ALD patients and 43 male alcohol abusers without liver complications defined as alcohol-dependent) were analyzed by polymerase chain reaction and hybridized with oligonucleotide microarray to detect the polymorphisms of the ethanol metabolizing enzymes genes. RESULTS: The frequencies of alcohol dehydrogenase gene 2 * 1 ( ADH2 * 1 ) allele were shown as 37.69%, 46.51% and 59.02% in control, alcohol-dependent and ALD groups respectively; while those of ADH2 * 2 allele were shown as 62.31 %, 53.49% and 40.98% respectively. No ADH2 * 3 was detected in any of the subjects. The frequency of ADH2 * 1 was significantly higher in alcoholisms (ALD group and alcohol-dependent group) than in healthy controls ( P

关键词： Oligonucleotide Array Sequence Analysis alcohol dehydrogenase Aldehyde Oxidoreductases Genotype Humans Isoenzymes Liver Diseases, alcoholic Male Polymorphism, Genetic

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