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Evidence on Invasion of Blood,adipose Tissues,Nervous System and Reproductive System of Mice After a Single Oral Exposure:Nanoplastics versus Microplastics

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Biomedical and Environmental Sciences 2022年第11期35卷 1025-1037页

作者： YANG Zuo Sen BAI Ying Long JIN Cui Hong NA Jun ZHANG Rui GAO Yuan PAN Guo Wei YAN Ling Jun SUN Wei Institute of Preventive Medicine China Medical UniversityShenyang 110122LiaoningChina Institute of Chronic Diseases Liaoning Provincial Center for Disease Control and PreventionShenyang 110000LiaoningChina Department of Maternal and Child Health School of Public HealthChina Medical UniversityShenyang 110122LiaoningChina Department of Toxicology School of Public HealthChina Medical UniversityShenyang 110122LiaoningChina Research Center for Universal Health School of Public HealthChina Medical UniversityShenyang 110122LiaoningChina

Objective This study was designed to provide the evidences on the toxicokinetics of microplastics(MPs)and nanoplastics(NPs)in the bodies of *** 100 nm,3μm,and 10μm fluorescent polystyrene(PS)beads were administered to mice once by gavage at a dose of 200 mg/kg body *** levels and change of fluorescence intensity in samples of blood,subcutaneous fat,perirenal fat,peritesticular fat,cerebrum,cerebellum,testis,and epididymis were measured at 0.5,1,2,and 4 h after administration using an IVIS Spectrum small-animal imaging *** examination,confocal laser scanning,and transmission electron microscope were performed to corroborate the *** After confirming fluorescent dye leaching and impact of pH value,increased levels of fluorescence intensity in blood,all adipose tissues examined,cerebrum,cerebellum,and testis were measured in the 100 nm group,but not in the 3 and 10μm groups except in the cerebellum and testis at 4 h for the 3μm PS *** presence of PS beads was further *** After a single oral exposure,NPs are absorbed rapidly in the blood,accumulate in adipose tissues,and penetrate the blood-brain/testis *** expected,the toxicokinetics of MPs is significantly size-dependent in mammals.

关键词： adipose accumulation Blood-brain barrier Blood-testis barrier Blood uptake Microplastics Nanoplastics

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Development of a Porcine cDNA Microarray:Analysis of Clenbuterol Responding Genes in Pig(Sus scrofa) Internal Organs

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Journal of Integrative Agriculture 2012年第11期11卷 1877-1883页

作者： ZHANG Jin GUO Wei SHEN Liang-cai LILT Qiu-yue DENG Xue-mei HU Xiao-xiang LINing College of Life Science and Biotechnology Hebei Normal University of Science & Technology Qinhuangdao 066600 P.R.China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 P.R.China College of Animal Science and Technology China Agricultural University Beijing 100193 P.R.China

Pig （Sus scrofa） fat accumulation can be reduced by feeding with high dosages of clenbuterol, but the molecular mechanism has not yet been explained. In our study, a porcine cDNA microarray representing 3 358 pig genes was successfully developed. This microarray is the first porcine DNA microarray in China and its false positive rate is 0.98%, which means the microarray platform is reliable. The microarray can be used to study gene expression profiles in multiple pig tissues because the present genes percentage of adipose, skeletal muscle, heart, liver, lung, kidney, and spleen were all more than 60%. This microarray was used to identify the genes responding to clenbuterol stimulation in pig internal organs, including heart, liver, lung, spleen, and kidney. Many genes were identified including enzymes involved in lipids metabolism （lipoprotein lipase up-regulated in liver, heart and lung, ATP-citrate lyase and carnitine palmitoyltransferase II precursor up-regulated in liver, succinyl-CoA up-regulated in lung, mitochondrial malate dehydrogenase down-regulated in spleen）, and signaling pathway genes （cAMP-protein kinase A signaling pathway was found up-regulated in liver, heart, lung, and kidney as reported previously, while transforming growth factor was found down-regulated in heart and lung）. However, no common gene responding to clenbuterol administration was found in all tissues. The expression levels of 14 genes were analyzed using real-time PCR with 82.1% of them induced to express similar magnitudes as in the microarray analyses. This work offers some understanding of how clenbuterol so effectively reduces pig adipose accumulation on the molecular level.

关键词： cDNA microarray pig clenbuterol adipose accumulation internal organ

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