Development of a Porcine cDNA Microarray:Analysis of Clenbuterol Responding Genes in Pig(Sus scrofa) Internal Organs

作     者：ZHANG Jin GUO Wei SHEN Liang-cai LILT Qiu-yue DENG Xue-mei HU Xiao-xiang LINing ZHANG Jin;GUO Wei;SHEN Liang-cai;LIU Qiu-yue;DENG Xue-mei;HU Xiao-xiang;LI Ning

作者机构：College of Life Science and Biotechnology Hebei Normal University of Science & Technology Qinhuangdao 066600 P.R.China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 P.R.China College of Animal Science and Technology China Agricultural University Beijing 100193 P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2012年第11卷第11期

页      面：1877-1883页

核心收录：

学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 07[理学] 08[工学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China (30800778 and 31072004) the Hebei Natural Science Foundation (C2009000871) the Hebei Educational Foundation,China (2009119) the Hebei Excellent Expert for Overseas Advanced Training Program (2009) Scientific Research Innovation Team Funds of Hebei Normal University of Sci & Tech,China 

主　　题：cDNA microarray pig clenbuterol adipose accumulation internal organ 

摘      要：Pig （Sus scrofa） fat accumulation can be reduced by feeding with high dosages of clenbuterol, but the molecular mechanism has not yet been explained. In our study, a porcine cDNA microarray representing 3 358 pig genes was successfully developed. This microarray is the first porcine DNA microarray in China and its false positive rate is 0.98%, which means the microarray platform is reliable. The microarray can be used to study gene expression profiles in multiple pig tissues because the present genes percentage of adipose, skeletal muscle, heart, liver, lung, kidney, and spleen were all more than 60%. This microarray was used to identify the genes responding to clenbuterol stimulation in pig internal organs, including heart, liver, lung, spleen, and kidney. Many genes were identified including enzymes involved in lipids metabolism （lipoprotein lipase up-regulated in liver, heart and lung, ATP-citrate lyase and carnitine palmitoyltransferase II precursor up-regulated in liver, succinyl-CoA up-regulated in lung, mitochondrial malate dehydrogenase down-regulated in spleen）, and signaling pathway genes （cAMP-protein kinase A signaling pathway was found up-regulated in liver, heart, lung, and kidney as reported previously, while transforming growth factor was found down-regulated in heart and lung）. However, no common gene responding to clenbuterol administration was found in all tissues. The expression levels of 14 genes were analyzed using real-time PCR with 82.1% of them induced to express similar magnitudes as in the microarray analyses. This work offers some understanding of how clenbuterol so effectively reduces pig adipose accumulation on the molecular level.