检索结果-南通市图书馆

Development of a high-fidelity Cas9-dependent adenine base editor (ABE) system for genome editing with high-fidelity Cas9 variants

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第3期10卷 705-707页

作者： Ruisha Hong Xionglei He State Key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhouGuangdong 510275China

One of the main reasons that hinders the application of base editors in the clinic is the trade-off between editing efficiency and editing fidelity.The off-target base editing includes Cas9-independent and Cas9-dependent manners.1 The Cas9-independent off-target results from the intrinsic deamination activity of the deaminase domain of a base editor,which affects random RNA or DNA molecules.

关键词： base fidelity adenine

Repetitive administration of cultured human CD34+cells improve adenine-induced kidney injury in mice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2023年第4期15卷 268-280页

作者： Takayasu Ohtake Shoichi Itaba Amankeldi A Salybekov Yin Sheng Tsutomu Sato Mitsuru Yanai Makoto Imagawa Shigeo Fujii Hiroki Kumagai Masamitsu Harata Takayuki Asahara Shuzo Kobayashi Regenerative Medicine The Center for Cell Therapy&Regenerative MedicineShonan Kamakura General HospitalKamakura 247-8533KanagawaJapan Kidney Disease and Transplant center Shonan Kamakura General HospitalKamakura 247-8533KanagawaJapan Regenerative Medicine Shonan Research Institute of Innovative MedicineKamakura 247-8533KanagawaJapan Kamakura Techno-science Inc. Kamakura 248-0036Japan Advanced Medicine Science Tokai University School of MedicineIsehara 259-1193Japan Department of Pathology Sapporo Tokushukai HospitalSapporo 004-0041Japan Department of Pathology Sapporo Medical CenterSapporo 004-0041Japan Human Life CORD Japan Inc Chuo-ku 103-0012TokyoJapan Cell Processing and Cell/Genome Analysis Center The Center for Cell Therapy&Regenerative MedicineShonan Kamakura General HospitalKamakura 247-8533KanagawaJapan Kidney Disease and Transplant Center Shonan Kamakura General HospitalKamakura 247-8533KanazawaJapan

BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(Padenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.

关键词： Chronic kidney disease CD34+cell adenine Tubulointerstitial injury Quality and quantity control culture Umbilical cord blood

adenine: an important drug scaffold for the design of antiviral agents

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2015年第5期5卷 431-441页

作者： Changyuan Wang Zhendong Song Haiqing Yu Kexin Liu Xiaodong Ma College of Pharmacy Dalian Medical University

adenine derivatives, in particular the scaffold bearing the acyclic nucleoside phosphonates(ANPS), possess signi fi cant antiviral and cytostatic activity. Till now, several effective adenine derivatives have been marketed for the treatment of HIV, HBV, CMV and other virus-infected diseases. These compounds are represented by tenofovir(PMPA), a medicine for both HIV and HBV, and adefovir as an anti-HBV agent. More than this, other analogs, such as GS9148, GS9131, and GS7340, are also wellknown anti-viral agents that have been progressed to the clinical studies for their excellent activity. In general, the structures of these compounds include an adenine nucleobase linked to a phosphonate side chain. Considerable structural modi fi cations on the scaffold itself and the peripheral sections were made.The structure-activity relationships(SARs) of this skeleton will provide valuable clues to identify more effective adenine derivatives as antiviral drugs. Here, we systematically summarized the SARs of the adenine derivatives, and gave important information for further optimizing this template.

关键词： Antiviral Structure–activity relationship adenine Acyclic nucleoside phosphonates Scaffold

Nicotinamide adenine dinucleotide phosphate oxidase activation and neuronal death after ischemic stroke

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2019年第6期14卷 948-953页

作者： Jiamei Shen Radhika Rastogi Xiaokun Geng Yuchuan Ding China-America Institute of Neuroscience Beijing Luhe HospitalCapital Medical University Department of Neurosurgery Wayne State University School of Medicine Department of Neurology Beijing Luhe HospitalCapital Medical University

Nicotinamide adenine dinucleotide phosphate oxidase(NOX) is a multisubunit enzyme complex that utilizes nicotinamide adenine dinucleotide phosphate to produce superoxide anions and other reactive oxygen species. Under normal circumstances, reactive oxygen species mediate a number of important cellular functions, including the facilitation of adaptive immunity. In pathogenic circumstances, however,excess reactive oxygen species generated by NOX promotes apoptotic cell death. In ischemic stroke, in particular, it has been shown that both NOX activation and derangements in glucose metabolism result in increased apoptosis. Moreover, recent studies have established that glucose, as a NOX substrate, plays a vital role in the pathogenesis of reperfusion injury. Thus, NOX inhibition has the potential to mitigate the deleterious impact of hyperglycemia on stroke. In this paper, we provide an overview of this research,coupled with a discussion of its implications for the development of NOX inhibition as a strategy for the treatment of ischemic stroke. Both inhibition using apocynin, as well as the prospect of developing more specific inhibitors based on what is now understood of the biology of NOX assembly and activation, will be highlighted in the course of our discussion.

关键词： nicotinamide adenine dinucleotide phosphate oxidase stroke nicotinamide adenine dinucleotide phosphate oxidase inhibitors reactive oxygen species ischemia/reperfusion neuroprotection hyperglycolysis NADPH NOX

Computational mechanistic investigation of radiation damage of adenine induced by hydroxyl radicals

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2018年第2期27卷 64-70页

作者：谈荣日刘慧宣寻大毛宗文军 College of Communication and Electronics Jiangxi Science & Technology Normal University Institute of High Energy Physics Chinese Academy of Sciences

The radiation damage of adenine base was studied by B3LYP and MP2 methods in the presence of hydroxyl radicals to probe the reactivities of five possible sites of an isolated adenine molecule. Both methods predict that the C8 site is the more vulnerable than the other sites. For its bonding covalently with the hydroxyl radicals, B3LYP predicts a barrierless pathway, while MP2 finds a transition state with an energy of 106.1 kJ/mol. For the hydroxylation at the C2 site, the barrier was calculated to be 165.3 kJ/mol using MP2 method. For the dehydrogenation reactions at five sites of adenine, B3LYP method predicts that the free energy barrier decreases in the order of H8 〉 H2 〉 HN62 〉 HN61 〉 HN9.

关键词： DNA damage radicals adenine dehydrogenation

Fabrication of poly(2,6-pyridinedicarboxylic acid)/MWNTs modified electrode for simultaneous determination of guanine and adenine in DNA

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2011年第8期22卷 981-984页

作者： Ke Qin Deng Chun Xiang Li Yu Lin Ling Guo Rong Xu Xiao Fang Li Chemistry and Chemical Engineering College Hunan University of Science and Technology Xiangtan 411201 China

The fabrication of poly（2,6-pyridinedicarboxylic acid）/MWNTs modified glass electrode（PPDA/MWNTs/GCE） was proposed and used for individual or simultaneous determination of guanine and adenine.The performances of the PPDA/MWNTs/GCE were characterized with cyclic voltammetry（CV）.The modified electrode exhibited enhanced electrocatalytic behavior and good stability for the detection of guanine and adenine.Differential pulse voltammetry（DPV） was used to determine the concentration of guanine,adenine.The detection limit（S/N = 3） for guanine and adenine was 0.045μmol/L and 0.05μmol/L,respectively.The electrochemical method for the measurement of guanine and adenine in calf thymus DNA was also developed with this modified electrode and the result was satisfactory.

关键词： Multi-wall carbon nanotubes 2 6-Pyridinedicarboxylic acid Guanine adenine Calf thymus dsDNA

Crystal Structure and Luminescence of a Cd(Ⅱ) Compound Based on the 3,3’,5,5’-Tetrafluorobiphenyl-4,4’-dicarboxylate and adenine Ligands

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Structural Chemistry 2020年第11期39卷 2041-2045页

作者： LIN Zhao-Ting WANG Yu-Ling LIU Qing-Yan College of Chemistry and Chemical Engineering Jiangxi Normal UniversityNanchang 330022China

A one-dimensional(1 D) coordination polymer, [Cd2(TFBPDC)2(ad)2(CH3 OH)2]n(1), based on the 3,3’,5,5’-tetrafluorobiphenyl-4,4’-dicarboxylate(TFBPDC2-) and adenine(ad) ligands, was synthesized. Compound 1 crystallizes in the triclinic system, space group P1 with a = 9.7067(3), b = 9.9501(3), c = 11.6903(4) ?, α = 93.036(3), β = 107.793(3), γ = 107.107(3)°, V = 1014.77(6) ?3, Z = 2, C40H26F8N10O10Cd2, Mr = 1183.51, Dc = 1.937 g/cm3, μ = 1.160 mm-1, F(000) = 584, the final R = 0.0284 and wR = 0.0635 for 3791 observed reflections with Ⅰ> 2s(I). In 1, a pair of adenine(ad) ligands bridge two octahedrally coordinated Cd(Ⅱ) atoms to form a dinuclear [Cd2(ad)2] unit, which is linked by TFBPDC2- to generate a 1 D double chain. The neighboring chains are connected by N-H×××O hydrogen bonds to give a two-dimensional(2 D) layer, which is further linked by the O-H×××N hydrogen bonds to give a three-dimensional(3 D) supramolecular framework. IR spectrum, thermogravimetric analysis, and powder X-ray diffraction of 1 were provided. Compound 1 shows luminescent emission.

关键词： cadmium coordination polymer 3 3’ 5 5’-tetrafluorobiphenyl-4 4’-dicarboxylate adenine luminescence crystal structure

CRISPR/Cas9-Mediated adenine Base Editing in Rice Genome

在线全文

学校读者我要写书评

暂无评论

Rice science 2019年第2期26卷 125-128,I0018-I0023页

作者： LI Hao QIN Ruiying LIU Xiaoshuang LIAO Shengxiang XU Rongfang YANG Jianbo WEI Pengcheng Key Laboratory of Rice Genetics & Breeding Institute of Rice ResearchAnhui Academy of Agricultural Science

Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor(ABE)-nC as9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.

关键词： Precise optimized adenine agricultural gene

Purines:From Diagnostic Biomarkers to Therapeutic Agents in Brain Injury

在线全文

学校读者我要写书评

暂无评论

Neuroscience Bulletin 2020年第11期36卷 1315-1326页

作者： Bruno G.Frenguelli Nicholas Dale School of Life Sciences The University WarwickCoventry CV47ALUK

The purines constitute a family of inter-related compounds that serve a broad range of important intracellular and extracellular biological functions.In particular,adenosine triphosphate(ATP)and its metabolite and precursor,adenosine,regulate a wide variety of cellular and systems-level physiological processes extending from ATP acting as the cellular energy currency,to the adenosine arising from the depletion of cellular ATP and responding to reduce energy demand and hence to preserve ATP during times of metabolic stress.This inter-relationship provides opportunities for both the diagnosis of energy depletion during conditions such as stroke,and the replenishment of ATP after such events.In this review we address these opportunities and the broad potential of purines as diagnostics and restorative agents.

关键词： Purines Adenosine ATP Biomarker Stroke Cerebral ischemia Traumatic brain injury adenine D-ribose