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actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats

Food Science and Human Wellness 2023年第6期12卷 1981-1990页

作者： Ali Y.Naoom Wenyi Kang Nora F.Ghanem Mohamed M.Abdel-Daim Fatma M.El-Demerdash Department of Medical Laboratory Techniques Imam Ja'afar Al-Sadiq UniversityAl-Muthanna 66002Iraq National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Department of Zoology Faculty of ScienceKafr ElSheikh UniversityKafr El Sheikh 33516Egypt Department of Pharmaceutical Sciences Pharmacy ProgramBatterjee Medical CollegeJeddah 21442Saudi Arabia Pharmacology Department Faculty of Veterinary MedicineSuez Canal UniversityIsmailia 41522Egypt Department of Environmental Studies Institute of Graduate Studies and ResearchAlexandria UniversityAlexandria 21526Egypt

This study aimed to evaluate the anti-hyperglycemic and antioxidant role of actinidia deliciosa(kiwifruits)aqueous extract in streptozotocin-treated *** were distributed into;control,*** aqueous extract(ADAE;1 g/kg orally),streptozotocin(STZ;50 mg/kg,i.p,single dose),and STZ plus ADAE,*** showed that ADAE had high antioxidant and radical scavenging *** in blood sugar level,lipid peroxidation(LPO),kidney function biomarkers,and perturbations in hematological parameters were observed in diabetic ***,enzymatic and non-enzymatic antioxidants,protein content,and alkaline phosphatase(ALP)activity ***,histological,immunohistochemical alpha-smooth muscle actin immunoreactivity(α-SMA-ir)and histochemical(collagen,total protein,DNA,and RNA)alterations were observed in rat ***,STZ produced upregulation of inflammatory associated genes(tumor necrosis factor-alpha;TNF-αand transforming growth factorβ1;TGF-β1)and triggered apoptosis by upregulating apoptotic related gene[Bcl2-associated X protein(Bax)]and downregulating anti-apoptotic related gene B-cell lymphoma-2(Bcl-2)based on real-time PCR ***,diabetic rats administered with ADAE showed significant restoration in LPO,antioxidant status,and biochemical indices besides tissue architecture,and genes improvement regarding STZ ***,*** has a valuable ameliorative infl uence and can restore glucose levels and improve kidney dysfunction in diabetic rats.

关键词： Diabetes mellitus actinidia deliciosa Inflammation Apoptosis Oxidative stress Nephropathy

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Characterization of Kiwifruit Xyloglucan

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Journal of Integrative Plant Biology 2009年第10期51卷 933-941页

作者： Xingjun Li Naoki Sakurai Donald J. Nevins Academy of the State Administration of Grains Beijing 100037 China Graduate School of Biosphere Science Hiroshima University Kagamiyama 1-7-1 Higashi-hiroshima 739-8521 Japan Department of Plant Sciences University of California Davis CA 95616 USA

Structural characteristics of xyloglucan are constant in the pericarp cell walls of kiwifruit （actinidia deliciosa） throughout fruit enlargement and maturation. Most of the xyioglucan （XG） persists in the cell walls of ripe kiwifruit. XG from the pericarp tissues of 36-h ethylene-treated kiwifruit was extracted as hemicellulose Ⅱ （HC-Ⅱ） with 4.28 M KOH containing 0.02% NaBH4, and purified using iodine precipitation and subsequent anion-exchange chromatography. This purifying protocol increased XG purity from 50 mol% in HC-Ⅱ fraction to 62 mol% in the purified XG powder. The molar ratio of glucose： xylose： galactose： fucose in the purified XG was 10： 6.9： 2.1： 0.3. Gel permeation chromatography indicated that purified XG had an average molecular-mass of 161 KDa, a value that exceeds the 95 KDa Mr determined for total polymeric sugars. Sugar linkage analysis confirmed the lack of fucose in the kiwifruit XG, but a small amount of arabinoxylan and low Mr glucomannan remained associated with this fraction.

关键词： actinidia deliciosa cell walls glucomannan hemicellulose sugar linkage analysis xyloglucan

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INTERSPECIFIC SOMATIC HYBRIDS IN actinidia

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Acta Botanica Sinica 1997年第12期39卷 1110-1117页

作者：肖尊安韩碧文北京师范大学生物系北京100875 中国农业大学生物学院北京100094

Protoplasts isolated from cotyledon-derived calli of actinidia chinensis var. chinensis (2n = 2x=58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or with mesophyll protoplasts of A. kolomikta (2n = 2x = 58), respectively. Randomly amplified polymorphic DNA (RAPD) markers and flow cytometry was used to confirm the occurrence of somatic hybrids. RAPD results with some primers surveyed indicated that one clone (A. chinensis + A. deliciosa) and four clones (A. chinensis + A. kolomikta) had RAPD banding patterns which combined the parental banding profiles. Ploidy levels of the (A. chinensis + A. deliciosa) clone were deduced as octoploid (2n = 8x), and the (A. chinensis + A. kolomikta) clones were tetraploid (2n = 4x), triploid (2n = 3x) or pentaploid (2n = 5x). The clones were confirmed as interspecific somatic hybrids in actinidia.

关键词： actinidia chinensis actinidia deliciosa actinidia kolomikta interspecific somatic hybrid

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