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Hyphal-mediated Infection is Determined by the Pair of Phosphorylation and Dephosphorylation to Achieve a Steady State Low Activity of atp citrate lyase in Magnaporthe oryzae

Hyphal-mediated Infection is Determined by the Pair of Phosp...

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中国植物病理学会2024年学术年会

作者： LIANG Hao WEI Yi Wang Shaowei ZHANG Shihong The Key Laboratory for Extreme-Environmental Microbiology College of Plant ProtectionShenyang Agricultural University Institute of Microbial Engineering Laboratory of Bioresource and Applied MicrobiologySchool of Life SciencesHenan University

Blast incited by Magnaporthe oryzae is a major problem in rice production,and the release of airborne-blast spores is a major determinant of disease ***,*** can also colonize rice underground organs under experiment conditions,although hyphae mediated root infection is difficult to distinguish in *** this study,we attempted to reveal the regulatory mechanisms of fungal infection by identifying the function and regulation mechanisms of the atp citrate *** protein phosphatase of ***,MoPTP7 was firstly *** being sensitive to exogenous HO,this deletion mutant of MoPTP7 also lost the ability of hyphal-mediated *** pull down and Co-IP approaches,a tyrosine kinase like protein MoTKL corresponding to MoPTP7 and a co acting substrate of this kinase and phosphatase,an atp citrate lyase subunit MoACLl,were *** the three genes function similarly in the blast *** combined analysis of phosphorylation level,enzyme activity,atp content,and fatty acid content indicates that the activity of ACL,regulated by its phosphorylation levels,affects the content of atp and fatty acids in *** phosphorylation state is balanced by joint actions of MoPTP7 and *** ACL activity varies with different infection forms,and hyphal infection required a lower level of ACL activity in compared with conidial *** summary,this study uncovered a new key regulatory mechanism for hyphal-mediated infection through a tyrosine phosphatase and a tyrosine kinase mediated atp citrate lyase activity regulation pathway.

关键词： hyphal-mediated infection conidial infection MoPTP7 MoTKL atp citrate lyase

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Key genes expression of reductive tricarboxylic acid cycle from deep-sea hydrothermal chemolithoautotrophic Caminibacter profundus in response to salinity, pH and O_2

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Acta Oceanologica Sinica 2013年第2期32卷 35-41页

作者： HE Peiqing LIU Yan YUE Wenjuan HUANG Xiaohang Key Laboratory of Marine Bioactive Substance State Oceanic Administration The First Institute of Oceanography State Oceanic Administration

CO2 fixation pathway of Caminibacter profundus, a chemolithoautotrophic e-Proteobacteria from deep-sea hydrothermal vent, was determined and characterized by genetic and enzymatic analyses. Gene expression of key enzymes for CO2 fixation in response to salinity, pH and O2 in Medium 829 were also investigated. The results demonstrate that C. profundus contained aclB, porA and oorA, the genes encoding key enzymes of reductive tricarboxylic acid （rTCA） cycle. However, genes fragments of cbbL and cbbMencoding key enzyme of Calvin cycle were not recovered. Key enzymatic activities of atp citrate lyase （ACL）, pyruvate： ferredoxin oxidoreductase （POR） and 2-oxoglutarate： ferredoxin oxidoreductase （OOR） were also present in C. profun- dus. The combination of genetic and enzymatic analyses confirm that C. profundus adopted rTCA cycle for carbon assimilation. The results of aclB and oorA relative expressions of C. profundus demonstrate that the ranges of environmental factors for high genes expression were sea salt 3.0%-5.0% （optimum 3.0%）, pH 5.0-6.5（optimum pH 6.5）, anaerobic to microaerobic conditions （optimum 1.0% 02）. Gene expression pat- terns under different conditions show similar patterns with bacterial growth, revealing that key rTCA cycle genes provided molecular basis for bacterial growth and propagation. Our results suggest that C. profun- dus could regulate key genes of rTCA cycle for carbon assimilation and energy metabolism in response to environmental fluctuations in hydrothermal vent.

关键词： Caminibacter profundus reductive tricarboxylic acid (rTCA) cycle atp citrate lyase pyruvate:ferredoxin oxidoreductase 2-oxoglutarate: ferredoxin oxidoreductase hydrothermal vent

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Isolation and Characterization of Contaminating Bacteria from Garcinia cambogia Extract: Methods to Reduce Microbial Load and Its Anti-Obesity Effect in Wistar Rats

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Natural Resources 2022年第7期13卷 147-170页

作者： Raj Kumar Bhosale Sasidharan Sakkan Srinivasa Murthy Venkatramanappa Shivaswamy Mathada Rudraiah Kumaraswamy Rampur Arulmozhi Sathiyanarayanan Deepa Mandlik Sanman Kolhe Sadanand Yewale Sriram Padmanabhan Herbal Division Sava Healthcare Limited Research Center MIDC Chinchwad India QC Division Sava Healthcare Limited KIADB Malur India Production Team Sava Healthcare Limited KIADB Malur India Department of Pharmacology Poona College of Pharmacy Bharati Vidyapeeth (Deemed to Be University) Pune India SAVA Healthcare Limited Research Center Pune India

Objectives: This study aimed to identify the contaminating bacteria in the extract of Garcinia cambogia, which is regularly used as a dietary supplement for addressing obesity in humans. Methods: The Garcinia cambogia extract was used and experiments were conducted to isolate the contaminating bacteria and antibiotic susceptibility was tested. The organism was identified using BIOLOG system. Such an extract was used in a placebo-controlled animal study when 6 eight adult male rats weighing between 200 and 220 g were randomly distributed into three groups (n = 3) and in test group 1, a single dose of 100 mg/kg bw of Garcinia cambogia extract was given while in the test group 2, 100 mg Garcinia cambogia extract + 116 mg Picrorhiza kurroa extract were administered through oral gavage. The normal control rats were given distilled water, and the treatment lasted for 30 days. Blood plasma and liver tissues were prepared for biochemical analysis and histology studies. Results: Nearly ~103 cfu/g of Bacillus atrophaeus was present in the Garcinia cambogia extract and we demonstrate >99% reduction in the microbial load with tetracycline. Such an extract at a dose of 100 mg/kg, showed weight loss in Wistar rats when administered orally for 1 month with no significant changes in liver histopathology. Picrorhiza kurroa, also known for its hepatoprotective properties, has been administered at a dose of 116 mg/kg along with Garcinia extract at 100 mg/kg orally and found to improve levels of hepatic enzymes as similar to control animals, although not statistically significant. Conclusions: The study revealed that Garcinia cambogia could prevent weight gain in Wistar rats when given orally and the weight gain in Garcinia-treated animals was almost 4 times less (7.31%), as against weight gain of 25.36% seen in vehicle control animals. The antibiotic susceptibility data indicated that the isolated bacterium is resistant to many antibiotics with a strong susceptibility to te

关键词： Garcinia cambogia Hydroxy Citric Acid Picrorhiza kurroa Liver Health atp citrate lyase

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