Hyphal-mediated Infection is Determined by the Pair of Phosphorylation and Dephosphorylation to Achieve a Steady State Low Activity of ATP Citrate Lyase in Magnaporthe oryzae
作者单位：The Key Laboratory for Extreme-Environmental MicrobiologyCollege of Plant ProtectionShenyang Agricultural University Institute of Microbial EngineeringLaboratory of Bioresource and Applied MicrobiologySchool of Life SciencesHenan University
会议名称：《中国植物病理学会2024年学术年会》
会议日期：2024年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：hyphal-mediated infection conidial infection MoPTP7 MoTKL ATP citrate lyase
摘 要：Blast incited by Magnaporthe oryzae is a major problem in rice production,and the release of airborne-blast spores is a major determinant of disease ***,*** can also colonize rice underground organs under experiment conditions,although hyphae mediated root infection is difficult to distinguish in *** this study,we attempted to reveal the regulatory mechanisms of fungal infection by identifying the function and regulation mechanisms of the ATP citrate *** protein phosphatase of ***,MoPTP7 was firstly *** being sensitive to exogenous HO,this deletion mutant of MoPTP7 also lost the ability of hyphal-mediated *** pull down and Co-IP approaches,a tyrosine kinase like protein MoTKL corresponding to MoPTP7 and a co acting substrate of this kinase and phosphatase,an ATP citrate lyase subunit MoACLl,were *** the three genes function similarly in the blast *** combined analysis of phosphorylation level,enzyme activity,ATP content,and fatty acid content indicates that the activity of ACL,regulated by its phosphorylation levels,affects the content of ATP and fatty acids in *** phosphorylation state is balanced by joint actions of MoPTP7 and *** ACL activity varies with different infection forms,and hyphal infection required a lower level of ACL activity in compared with conidial *** summary,this study uncovered a new key regulatory mechanism for hyphal-mediated infection through a tyrosine phosphatase and a tyrosine kinase mediated ATP citrate lyase activity regulation pathway.