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针刺研究 1994年第Z1期 15-16页

EFFECT OF CENTRAL ach IN ACUPUNCTURE ANALGESIA

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针刺研究 1989年第Z1期 173-176页

作者： Ai Mingkang Guan Xinming Wang Caiyuan Ru Liqiang Research Department of Mechanism of Acupuncture Analgesia

Acctylcholine (ach) is the neuro transmitter which carliest emerges no matter in development of species or in evolution of individual. In some lower animalwhole sensory afferent nerve is cholinedergic, in advanced animals part ofnociceptive sensory neuroanatomic pathways is corresponding to ascending reti-cular exciting system. So it is possible that ach is considered as one of impo-rtant neurotransmitters relating to acupuncture analgesia.

关键词： achE EFFECT OF CENTRAL ach IN ACUPUNCTURE ANALGESIA ach

INFLUENCE OF ELECTROACUPUNCTURE ON THE METABOLISM OF ach IN THE DORSAL HORN OF SPINAL CORD

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针刺研究 1989年第Z1期 180-181页

作者： Shi Jing Ai Minkang Guan Xinmin Research Department of Acupuncture Analgesic Mechanism Department of Neurobiology Tongji Medical University

Dorsal horn of spinal cord is an important relay of primary afferent. Inorder to investigate the effect of ach in spinal cord on electroacupunctureanalgesia, the ach contant,ChAC and achE activity were measured. 72 rats wereused in this experiment and separated into two groups, A and B. Eachgroup was subdivided into electroacupuncture group and control group. Theach content and ChAC activity in dorsal horn of animal group A; andachE activity in those of group B were investigated. After electroacupun-cture induction for 10, 20, 30 minutes, it was found that the effectiveness ofacupuncture analgesia direct relation to intensity of electroacupuncture(relative

关键词： achE INFLUENCE OF ELECTROACUPUNCTURE ON THE METABOLISM OF ach IN THE DORSAL HORN OF SPINAL CORD ach

Effect of ach Locally Applied to Spinal Ganglia on the Primary Somestetic Afferents

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针刺研究 1994年第Z1期 103-104页

EFFECT OF ELECTROACUPUNCTURE ON ach CONTENT AND achE ACTIVITY IN THE SPINAL GANGLIA OF RAT

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针刺研究 1989年第Z1期 182-183页

作者： Zhang Yuwen Zhang Nana Qian Hui Liu Xiaochun Guan Xinmin Research Department of Acupuncture Analgesia Mechanism Tong-ji Medical University

The present exneriment war carried out for the observations on changes ofach content and achE activity in spinal ganglia after dorsal rhiztomy andelectroacupuncture by means of physiological and biochemical methods andaimed at investigations into relationship between peripheral and acupunctureinformation transmitted through primary afferent. The results indicate:1. It was proved that ach exists in spinal *** average value of ach content measured bioassaily is 0.424±0.109ngper mg of humid tissue. The data is normal distribution.

关键词： achE ach EFFECT OF ELECTROACUPUNCTURE ON ach CONTENT AND achE ACTIVITY IN THE SPINAL GANGLIA OF RAT

载体预处理对蜂窝状V_2O_5/ach低温脱硫脱硝活性的影响

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新型炭材料 2004年第3期19卷 179-185页

作者：王艳莉刘振宇黄张根刘清雅中国科学院山西煤炭化学研究所煤转化国家重点实验室

　经不同预处理条件制得的蜂窝状活性炭(ach),等体积浸渍偏钒酸铵和草酸的混合溶液,经干燥、煅烧和预氧化后制得新型V2O5 ach催化剂。实验结果表明,850℃下水蒸气活化可提高2%V2O5 ach催化剂在200℃下的脱硫活性,但基本不改变脱硝活性;... 详细信息

　经不同预处理条件制得的蜂窝状活性炭(ach),等体积浸渍偏钒酸铵和草酸的混合溶液,经干燥、煅烧和预氧化后制得新型V2O5 ach催化剂。实验结果表明,850℃下水蒸气活化可提高2%V2O5 ach催化剂在200℃下的脱硫活性,但基本不改变脱硝活性;NH3再生后催化剂的脱硝活性显著提高,对SO2的吸附也有所增加。活化温度从850℃升至950℃,相应催化剂的脱硫脱硝活性略微降低;活化时水蒸气体积含量从28%增至80%,催化剂的脱硫脱硝活性也有不同程度的下降。在28%的水蒸气含量和850℃活化温度的最佳预处理条件下,经第二次再生后的2%V2O5 ach催化剂的SO2转化率降为80%时的吸附硫容为4.9%,同时脱硝率达93%。

关键词：催化剂 V2O5 活化温度活性变脱水蒸气硫容 ach 改变预处理

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ach对人垂体腺瘤细胞内蛋白激酶C、胞内游离钙及cAMP/cGMP的影响

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生理学报 2003年第2期55卷 165-170页

作者：迟素敏李承新刘亚莉朱运龙顾建文第四军医大学生理学教研室西安710032 第四军医大学西京医院全军皮肤性病中心西安710032 第四军医大学西京医院脑外科西安710032

我们曾发现ach可明显地抑制垂体腺瘤细胞的增殖代谢 ,为深入探讨ach抑制垂体腺瘤细胞增殖作用的机制 ,观察了ach作用后垂体腺瘤细胞内蛋白激酶C (PKC)、[Ca2 +]i及cAMP/cGMP的变化。结果发现 :( 1)与空白处理组相比 ,使用PKC的激动剂PM... 详细信息

我们曾发现ach可明显地抑制垂体腺瘤细胞的增殖代谢 ,为深入探讨ach抑制垂体腺瘤细胞增殖作用的机制 ,观察了ach作用后垂体腺瘤细胞内蛋白激酶C (PKC)、[Ca2 +]i及cAMP/cGMP的变化。结果发现 :( 1)与空白处理组相比 ,使用PKC的激动剂PMA处理培养的人垂体腺瘤细胞时可使胞浆、胞膜和细胞总PKC活性浓度均升高 ,但ach ( 10 μmol/L)作用 15min后 ,胞浆、胞膜和细胞总PKC活性均下降 ,且此作用可被阿托品阻断 ;( 2 )ach ( 10 μmol/L)作用于单个人垂体腺瘤细胞后 ,立即使垂体腺瘤细胞 [Ca2 +]i 相对水平降低 ,但此作用可被阿托品阻断 ;( 3 )ach作用于人垂体腺瘤细胞 15min后 ,胞内cAMP水平均明显升高 ,而cGMP没有改变。该结果为探讨ach抑制垂体腺瘤细胞增殖的分子机制提供了重要线索 ,同时提示。

关键词： ach 垂体腺瘤蛋白激酶C [Ca^2+]i cAMP/cGMP

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Involvement of pylorus n NOS and ach in delayed gastric emptying of 6-OHDA rats

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Involvement of pylorus n NOS and ACh in delayed gastric empt...

参归煎剂对双侧NBM损伤痴呆大鼠海马ach含量及ChAT活性的影响

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：樊瑞芳郑丽飞朱进霞首都医科大学基础医学院生理与病理生理学系消化生理实验室

Background Parkinson disease(PD)is a progressive neurodegenerative disease and always happened to the *** addition to the classical cardinal motor symptoms PD patients always suffer from gastrointestinal(GI)disorders(such as gastroparesis and constipation),but the mechanism remains *** we have reported that 6-OHDA rat model,a classic PD model,exhibits typical GI symptoms like delayed gastric emptying with enteric NO/acetylcholine(ach)alteration in *** aim of the present study is to investigate the regulation of enteric neural nitric oxide synthestase(n NOS)and cholinergic system on the pylorus function and the mechanism underlying the gastric emptying disorder in 6-OHDA *** and methods Sprague-Dawley rats,weight 20050g,were treated with 6-hydroxydopamine(6-OHDA)by microinjected into bilateral substantia nigra,which referred to6-OHDA *** diet gastric emptying test,Nissl staining,Immunofluorescence,Western blot,and in vitro pylorus motility recording were used in this *** Solid diet gastric emptying test indicated that the 6-OHDA rats had a delayed gastric ***-vitro study of smooth muscle strip contraction,6-OHDA rats displayed a higher basal tone(control:507.9±31.8mg,6-OHDA:679.1±36.2mg,n=13,P<0.01),but lower basal pylorus motility index(control:64.3±5.2mg*s,6-OHDA:37.7±3.6 mg*s,n=4,P<0.01).L-NAME(100μmol/L),a non-selective inhibitor of NOS,significantly increased pylorus motility including basal tone and contractive amplitude,but the efficacy is weaker in 6-OHDA rats(control:193.3±21.8mg,6-OHDA:121.4±14.7mg,n=5,P<0.05).Bethanechol chloride significantly increased motility index,but the reaction is also smaller in 6-OHDA rats(the amplification:control:2.1±0.2,6-OHDA:1.3±0.07,n=5,P<0.05).The role of L-NAME or Bethanechol chloride in the pylorus motility of 6-OHDA model was *** exhibited an extensive distribution of n NOS in pylorus enteric nervous *** blot showed a de

关键词： nNOS ach pylorus motility gastric emptying 6-OHDA model

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中国老年学杂志 2000年第6期20卷 374-375页

作者：陈声武荣大奇崔立波史蕴萍王丽娟王岩周庆伟宗瑞义谢湘林长春中医学院附属医院长春130021 吉林省卫生信息研究所白求恩医科大学

目的　探讨参归煎剂改善实验性痴呆动物模型学习记忆障碍的作用机制。方法　向大鼠基底前脑 Meynert基底核 (NBM)注入鹅蕈氨酸 (Ib) ,造成中枢胆碱能系统障碍性的阿尔茨海默病 (AD)动物模型。通过放射免疫分析方法 ,观察参归煎剂对该模... 详细信息

目的　探讨参归煎剂改善实验性痴呆动物模型学习记忆障碍的作用机制。方法　向大鼠基底前脑 Meynert基底核 (NBM)注入鹅蕈氨酸 (Ib) ,造成中枢胆碱能系统障碍性的阿尔茨海默病 (AD)动物模型。通过放射免疫分析方法 ,观察参归煎剂对该模型大鼠海马内乙酰胆碱 (ach)含量和胆碱乙酰转移酶 (Ch AT)活性的影响。结果　给药组大鼠海马 ach含量及 Ch AT活性均明显高于模型组。结论　参归煎剂对 AD模型大鼠学习记忆功能障碍的改善作用 ,可能与其对中枢胆碱能系统的保护作用或者说与其增强中枢胆碱能系统的功能活动有关。

关键词：参归煎剂阿尔茨海默病 ach ChAT 海马

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INHIBITORY EFFECTS OF VITAM IN E SUCCINATE ON BENZO(A) PYRENE-INDUCED FOR ESTOM ach CARCINOGENESIS IN FEMALE MICE

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癌变·畸变·突变 2001年第4期 239-240页

作者： WU Kun YU Juan YU Wu Jian Harbin Medical University

This study is a part of an effort to develop effective chemoprevention for carcinogenesis of the forestomach. Vitamin E succinate(VES) administered by oral gavage and by intraperitioneal (IP) injections was used for the studies. Experimental design involved treating mice with Benzo(a) pyrene and VES(1.25 g/kg and 2.5 g/kg for gavage; 20 mg/kg for IP injections) for 4 weeks in order to study the

关键词： ach PYRENE-INDUCED FOR ESTOM ach CARCINOGENESIS IN FEMALE MICE INHIBITORY EFFECTS OF VITAM IN E SUCCINATE ON BENZO