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Long-term culture of primary porcine mature hepatocytes in the medium supplemented with ascorbic acid 2-phosphate

Natural Science 2010年第11期2卷 1264-1273页

作者： Yohichi Kumaki Iku Kumaki Xiaomei Guo Weilin Shang Toshie Koyama Ai Okamura Yoshiaki Shiba Toshiyuki Mukaiyama Noriko Sasaki Makoto Kodama 不详

In this study, the effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. a). Asc2P increased the porcine, but not rat, albumin secretion and mRNA expression. The enhancing effect of Asc2P on porcine C/EBP alpha mRNA was observed in porcine mature hepatocytes. These data suggested that Asc2P played an important role in the regulation of porcine albumin mRNA level. b). The enhancing effect of Asc2P on ammonium metabolic activity was also observed in porcine, but not rat, mature hepatocytes. The porcine ornithine transcarbamylase (OTC) and arginase mRNAs were augmented by Asc2P, indicating that Asc2P had a direct effect on the urea cycle. c). The porcine collagen type I and type III mRNA, but not type XII mRNA, were detected as well, sugessting that Asc2P did not have the effect on the non-parenchymal hepatocytes to induce collagen type I and III mRNA expression. d). Our RT-PCR analysis demonstrated that the porcine hepatocytes expressed the sodium-ascorbate co-transporters SVCT1 and SVCT2, however, the intensities of porcine sodium-ascorbate co-transporters SVCT1 and SVCT2 bands were not changed markedly. These findings indicated that the Asc2P had no effect on SVCT1 and SVCT2 mRNA expression. e). The enhancing effect of Asc2P on porcine albumin mRNA was inhibited by staurosporine, a portein kinase inhibitor. We conclude that the enhanced albumin mRNA by Asc2P might be due to activation of tyrosine protein kinase and/or PKC and the Asc2P enhanced porcine albumin mRNA mainly at the transcriptional step.

关键词： Albumin Secretion Ammonium Metabolic Activity Ascorbic Acid 2-phosphate Porcine Hepatocytes Reverse Transcriptase-Polymerase Chain Reaction

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Enhanced Iontophoretic Delivery of Magnesium Ascorbyl 2-phosphate and Sodium Fluorescein to Hairless and Hairy Mouse Skin

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第4期2卷 283-287页

作者： Min Young Kang Su Hee Kim Young Kwan Sung Moonkyu Kim Jung Chul Kim Insook Han Department of Immunology School of Medicine Kyungpook National University Daegu South Korea.

We recently reported that L-ascorbic acid 2-phosphate (AP) stimulates the growth of human dermal papilla (DP) cells, induces secretion of IGF-1 from the DP cells to promote hair shafts elongation in cultured human hair follicles, and triggers early progression from the telogen to anagen phase in mice. Since the magnesium salt of AP (APMg) is a highly hydrophilic ionic molecule, it is not easy to deliver this reagent to the skin or hair follicles by topical application alone. In order to enhance skin penetration of APMg without changing any molecular properties, a non-invasive iontophoretic delivery method was introduced. Iontophoresis of the negatively charged APMg under the electrode bearing same charge (cathode) significantly enhanced the in vitro penetration of APMg into a Franz cell equipped with mouse dorsal skin. In contrast, iontophoretic movement with the anode inhibited APMg penetration achieved with passive diffusion alone. The effect of iontophoresis on enhancing the penetration of APMg was also found to be much higher in the skin of hairy mice (3 - 8 times) compared to hairless mice (1.5 - 2.5 times). These findings indicated that iontophoretic movement induced the transfollicular pathway more strongly and effectively than the transdermal pathway. This phenomena was also demonstrated by the in vivo iontophoretic delivery of sodium fluorescein using hairy and hairless mice. The degree of iontophoretic enhancement during APMg penetration was also dependent on various conditions such as current density and application duration.

关键词： L-Ascorbic Acid 2-phosphate Sodium Fluorescein Iontophoresis Transfollicular Pathway

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Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet

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Health 2013年第8期5卷 9-15页

作者： Mai Kobayashi Isao Matsui-Yuasa Maiko Fukuda-Shimizu Yoshinobu Mandai Masaki Tabuchi Hiroshi Munakata Akiko Kojima-Yuasa Department of Biochemistry Kinki University Medical School Osaka-Sayama Japan Department of Food and Human Health Sciences Graduate School of Human Life Science Osaka City University Osaka Japan Nitta Gelatin Inc. Yao Japan

Mangoes (Mangifera indica L.) are one of the most important tropical foods. The seed is one of the main by-products of mango processing. Therefore, it is important to find an economically viable use for this waste (e.g., as a food additive or supplement with high nutraceutical value). We investigated the anti-obesity effects of mango seed kernel extract with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet (HFD)-induced obesity rat model. MSKE-W caused a significant decrease in the activity of glycerol 2-phosphate dehydrogenase in 3T3-L1 adipocytes without eliciting cell cytotoxicity and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPARγ and C/EBPα. In the animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W tended to be lower than that in rats fed an HFD alone. Furthermore, histological examination of rat livers from an HFD showed steatohepatitis. However, rats on an HFD containning 1% MSKE-W showed no histopathological changes in liver tissue. Our results indicate that MSKE-W influences anti-obesity effects, both in vitro and in vivo, and suggest that MSKE-W provides a novel preventive potential against obesity.

关键词： Mago Seed Kernel Glycerol 2-phosphate Dehydrogenase (GPDH) Peroxisome Proliferator-Activated Receptor γ (PPARγ) High Fat Diet-Induced Obesity Rat Model 3T3-L1 Adipocytes

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