Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet

作     者：Mai Kobayashi Isao Matsui-Yuasa Maiko Fukuda-Shimizu Yoshinobu Mandai Masaki Tabuchi Hiroshi Munakata Akiko Kojima-Yuasa 

作者机构：Department of Biochemistry Kinki University Medical School Osaka-Sayama Japan Department of Food and Human Health Sciences Graduate School of Human Life Science Osaka City University Osaka Japan Nitta Gelatin Inc. Yao Japan 

出 版 物：《Health》 (健康（英文）)

年 卷 期：2013年第5卷第8期

页      面：9-15页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Mago Seed Kernel Glycerol 2-Phosphate Dehydrogenase (GPDH) Peroxisome Proliferator-Activated Receptor γ (PPARγ) High Fat Diet-Induced Obesity Rat Model 3T3-L1 Adipocytes 

摘      要：Mangoes (Mangifera indica L.) are one of the most important tropical foods. The seed is one of the main by-products of mango processing. Therefore, it is important to find an economically viable use for this waste (e.g., as a food additive or supplement with high nutraceutical value). We investigated the anti-obesity effects of mango seed kernel extract with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet (HFD)-induced obesity rat model. MSKE-W caused a significant decrease in the activity of glycerol 2-phosphate dehydrogenase in 3T3-L1 adipocytes without eliciting cell cytotoxicity and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPARγ and C/EBPα. In the animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W tended to be lower than that in rats fed an HFD alone. Furthermore, histological examination of rat livers from an HFD showed steatohepatitis. However, rats on an HFD containning 1% MSKE-W showed no histopathological changes in liver tissue. Our results indicate that MSKE-W influences anti-obesity effects, both in vitro and in vivo, and suggest that MSKE-W provides a novel preventive potential against obesity.