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Relaxation of rabbit cavernous smooth muscle to 17β-estradiol: a non-genomic, NO-independent mechanism

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Asian Journal of Andrology 2004年第2期6卷 127-131页

作者： Sae-Chul Kim Kyung-Kun Seo Soon-Chul Myung Moo Yeol Lee Department of Urology College of Medicine Chung-Ang University Seoul 140-757 KoreaDepartment of Urology College of Medicine Chung-Ang University Seoul 140-757 KoreaDepartment of Urology College of Medicine Chung-Ang University Seoul 140-757 KoreaDepartment of Physiology College of Medicine Chung-Ang University Seoul 140-757 Korea

Aim: To investigate whether estrogen was involved in relaxation of rabbit cavernous smooth muscle. Methods: Relaxation response of the rabbit cavernous smooth muscles to 17β-estradiol (0.3, 3, 30 and 300 nmol/L) were observed in vitro. The response of the muscle strips to estrogen after incubation with either actinomycin D (10μmol/L) or L-NAME (10μmol/L) were also evaluated. Inside-out mode of patch clamp in a single smooth muscle cell was applied to investigate the Maxi-K channel activities. Results: Estrogen caused a dose-dependent relaxation of the strips precontracted with norepinephrine. The maximal response was noted about 10 minutes after treatment. The estrogen-induced relaxation was prevented by neither actinomycin D nor L-NAME, suggesting that the response was not mediated by gene transcription or nitric oxide (NO). Application of 17β-estradiol increased the Maxi-K channel activities. Conclusion: 17β-estradiol may be involved in relaxation of rabbit cavernous smooth muscles via a non genomic and NO independent mechanism. 17β-estradiol stimulates Maxi-K channel of the rabbit cavernous myocyte.

关键词： 17β-estradiol cavernous smooth muscles Maxi-K channel relaxation

Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages

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Chinese Journal of Oceanology and Limnology 2017年第3期35卷 613-623页

作者：胡鹏刘滨孟振刘新富贾玉东杨志雷霁霖 Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Municipal Key Laboratory of Marine Fish Breeding and Biotechnology Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Tianyuan Aquaculture Co. Ltd.of Yantai Economic Development ZoneYantai 264000China

The aim of the present study was to investigate the long-term effects of 17l）-estradiol （E2） exposure on gonadal development in the tiger puffer （Taktfugu rubripes）, which has a genetic sex determination system of male homogametic XY-XX. Tiger puffer larvae were exposed to 1, 10 and 100 μg/L E2 from 15 to 100 days post-hatch （dph） and then maintained in clean seawater until 400 dph. Changes in sex ratio, gonadal structure and gonadosomatic index （GSI） were monitored at 100, 160, 270 and 400 dph. Sex-associated single nucleotide polymorphism （SNP） markers were used to analyze the genetic sex of samples, except those at 100 dph. Exposure had a positive effect on the conversion of genetically male gonads into phenotypically female gonads at 100 dph. However, gonads from 60% of genetic XY males in the 1-μg/L E2 group and 100% in the 10-μg/L E2 group developed intersexual gonads at 160 dph; gonads of all genetic XY males in the two treatment groups reverted to testis by 270 dph. While 38%, 57% and 44% of gonads of XY fish in the 100-gg/L E2 group reverted to intersexual gonads at 160, 270 and 400 dph, respectively, none reverted to testis after E2 treatment. In addition, E2 exposure inhibited gonadal growth of both genetic sexes, as indicated by the clear dose-dependent decrease in GSI at 270 and 400 dph. The results showed that exposure to E2 during the early life stages of tiger puffer disrupted gonadal development, but that fish recovered after migration to clean seawater. The study suggests the potential use of tiger puffer as a valuable indicator species to evaluate the effects of environmental estrogens on marine fish, thereby protecting valuable fishery resources.

关键词： Takifugu rubripes 17β-estradiol sex reversal gonadal structure morphological recovery

Xenoestrogens challenge 17β-estradiol protective effects in colon cancer

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World Journal of Gastrointestinal Oncology 2014年第3期6卷 67-73页

作者： Maria Marino Department of Science University Roma TreI-00146 RomaItaly

Several epidemiological,cellular,and molecular studies demonstrate the role of environmental chemicals with endocrine disrupting activities,typical of Westernized societies,in the pathogenesis of numerous diseases including *** this information,the design and execution of studies on endocrine disruptors are not yet cognizant that the specific actions of individual hormones often change with development and ageing,they may be different in males and females and may be mediated by different receptors isoforms expressed in different tissues or at different life *** statements are particularly true when assessing the hazard of endocrine disruptors against 17β-estradiol(E2)actions in that this hormone is crucial determinant of sexrelated differences in anatomical,physiological,and behavioral traits which characterize male and female ***,E2 is also involved in *** oncogenic effects of E2 have been investigated extensively in breast and ovarian cancers where hormone-receptor modulators are now an integral part of targeted *** is known about the E2preventive signalling in colorectal cancer,although this disease is more common in men than women,the difference being more striking amongst pre-menopausal women and age-matched *** review aims to dissect the role and action mechanisms of E2 in colorectal cancer evaluating the ability of estrogen disruptors(i.e.,xenoestrogens)in impair these E2 *** discussed here lead to define the possible role of xenoestrogens in the impairment and/or activation of E2signals important for colorectal cancer prevention.

关键词： 17β-estradiol Estrogen receptors Xenoestrogens Bisphenol A Flavonoids Colorectal cancer

17β-estradiol通过促进PSD95/nNOS耦联抑制皮层海马神经元的存活

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南京医科大学学报（自然科学版） 2011年第5期31卷 702-705页

作者：邬丹莲胡瑶朱明媚朱东亚南京医科大学药学院药理教研室江苏南京210029 南京医科大学附属南京儿童医院药剂科江苏南京210008

目的:检测17β-estradiol对皮层海马神经元存活的影响,并判断其与nNOS/PSD95耦联的关系。方法:采用原代培养的ICR小鼠皮层海马神经元,培养7天后分溶剂对照组和给药组分别给予溶剂DMSO和浓度为10μmol/L的17β-estradiol,LDH法判断细胞损... 详细信息

目的:检测17β-estradiol对皮层海马神经元存活的影响,并判断其与nNOS/PSD95耦联的关系。方法:采用原代培养的ICR小鼠皮层海马神经元,培养7天后分溶剂对照组和给药组分别给予溶剂DMSO和浓度为10μmol/L的17β-estradiol,LDH法判断细胞损伤,测定给药30 min后细胞死亡情况,并拍摄光镜照片观察细胞形态。同时利用免疫共沉淀法检测DMSO组,10nmol/L17β-estradiol组,10μmol/L 17β-estradiol组的nNOS和PSD95的耦联情况,观察药物对其影响。结果:给药30 min后,与对照组相比,10μmol/L17β-estradiol组LDH漏出率增加,同时nNOS/PSD95耦联增加,而10 nmol/L 17β-estradiol对nNOS/PSD95耦联并没有影响。结论:浓度为10μmol/L的17β-estradiol会损伤皮层海马神经元,而且这一结果可能是通过促进nNOS/PSD95耦联实现的。

关键词： nNOS PSD95 雌激素 17β-estradiol 细胞死亡

17β-estradiol protects SD rat retinal neurons from light damage by activating PI3K/Akt and NF-κB signal pathways

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17β-Estradiol protects SD rat retinal neurons from light da...

Removal of 17β-estradiol by Electro-Fenton Process

中国生物化学与分子生物学会第十届会员代表大会暨全国学术会议

作者： Ming Shu Mo Shao Lan Wang Hongbo Li Yan Feng Zhaoliang Zhu Baoying Wang Ruochen Zhu Shuhong Wang Yan Han Xiaorui Yu Department of Genetics and Molecular Biology School of MedicineXi’an Jiaotong University

Our previous studies have proved that the phosphatidylinositol-3-kinase(PI3K)/Akt signal pathway was involved in the neuroprotection of 17β-estradiol(βE2) against H2O2-induced retinal neurons apoptosis in *** aim of this study is to observe whetherβE2 can also protect retinal neurons from light damage in vivo by activating the PI3K/Akt signal *** used 180 to 230 g female SD rats exposed to persistent white light after ovariectomy 2 weeks as experimental model and used Electroretinogram (ERG),DNA ladder,TUNEL,HE staining,Western blotting and Immunofluorescence techniques to investigate the neuroprotection ofβ*** results showed that SD rats were exposed to 8000 lux white light for 12 h resulted in retinal function decrease and photoreceptor cells apoptosis, and 10-4M and 10-5MβE2 by intravitreal injection all significantly enhanced the photosensitive function of retinal by preventing neuronal *** mechanism research found that the neuroprotection ofβE2 involved in increasing the phosphorylation of Akt which was partly blocked by the pretreatment with PI3K inhibitor LY294002 for 30 min beforeβE2 *** cleaved caspase-3 as an activation form of caspase-3 was observed after light damage and the administration ofβE2 can attenuate the activation of caspase-3,while pretreatment with LY294002 can inhibit the attenuation ofβ*** results showed that NF-κB translocated from cytoplasm to nucleus afterβE2 injection,and LY294002 could also inhibit partly this *** present results indicated thatβE2 has a neuroprotective effect against light-induced photoreceptor cells apoptosis,and the PI3K/Akt pathway plays an important role in this neuroprotection,which involved in translocation of NF-κB from cytoplasm to nucleus and inhibited the activation of caspase- 3,but the detailed mechanism of theβE2 neuroprotection is being further study.

关键词： 17β-estradiol retinal light damage neuroprotection apoptosis

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Materials Sciences and Applications 2012年第12期3卷 880-886页

作者： I. Naimi N. Bellakhal Department of Chemistry and Biology National Institute of Applied Sciences and Technology Tunis Tunisia

The Electrochemical advanced oxidation method “Electro-Fenton” has been applied to remove 17β-estradiol (17β)- estra-1,3,5(10)-triéne-3,17-diol) in aqueous-acetonitrile mixture. This endocrine disrupting is a steroid hormone, releases from humans, animals and residual pharmaceuticals into the environmental water and usually causes suspected undesirable effects in aquatic organisms. The degradation of this organic compound by Electro-Fenton process was showed using a carbon felt cathode and platinum anode. The evolution of the concentration during treatment was followed up by high performance liquid chromatography (HPLC). The influence of operating conditions on the degradation of 17β-estradiol by Electro-Fenton step, such as initial concentration and catalyst concentration, has been investi- gated and discussed. We showed that the degradation reaction obeyed apparent first-order reaction kinetics, with absolute rate constant determined as 5.12 × 109 M–1 s–1 by competitive kinetics method taking Benzoic Acid as reference compound. The results confirm the efficiency of the Electro-Fenton process to degrade organic pollutant in aqueous-acetonitrile mixture.

关键词： 17β-estradiol Endocrine Disrupting Chemicals Electro-Fenton Hydroxyl Radicals Degradation

Effects of Ovariectomy and 17β-estradiol Replacement on the Activity of Dopamine D2 Receptors in the Selection of Macronutrients Carbohydrates, Lipids and Proteins in Females Rats

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Journal of Biosciences and Medicines 2023年第5期11卷 76-110页

作者： Brahima Bamba Seydou Silué Tiémélé Eugène Atayi Antoine Némé Tako Laboratoire de Neurosciences UFR Biosciences Université Félix Houphou&eumlt-Boigny Abidjan C&ocircte d’Ivoire Service de Neurologie UFR Des Sciences Médicales Centre Hospitalier et Universitaire (CHU) de Cocody Abidjan C&ocircte d’Ivoire

17β-estradiol modulates the activity of D2 receptors in the regulation of food intake and body weight. The functional lack of 17β-estradiol in postmenopausal women could create a dietary imbalance and cause body weight gain. This study aimed to better understand the interferences that could exist between 17β-estradiol, D2 receptors and the selection of carbohydrate, fat and protein consumption, as well as their consequences on body weight gain by using an animal model of the menopause. Ovariectomy exacerbates the consumption of foods rich in lipids. Thus confirming an inhibitory action of 17β-estradiol (E2) on the consumption of these types of foods. This consumption stimulates body weight gain, which is promoted by the high caloric content of these foods and not by the amount consumed. Our results showed a direct involvement of D2 receptors in food choice. This choice would be made according to the two (2) isoforms of the D2 receptors. The D2/BR isoform directs towards a high carbohydrate consumption, without causing a gain in body weight. While D2/SUL, promotes high fat food consumption, causing an increase in body weight. In women, 17β-estradiol modulates the activity ratio between these two D2 receptor isoforms to ensure energy and homeostatic balance, stabilizing food intake and body weight.

关键词： 17β-estradiol D2 Receptors Bromocriptine Sulpiride Carbohydrates Lipids Proteins Body Weight Menopause Obesity

Induction of testis-ova in nile tilapia (Oreochromis niloticus) exposed to 17β-estradiol

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Natural Science 2011年第3期3卷 227-233页

作者： Piya Kosai Wannee Jiraungkoorskul Chaivira Sachamahithinant Kanitta Jiraungkoorskul 不详

The efficacy of Endocrine Disrupting Compounds (EDCs), 17β-estradiol was tested on the fish Oreochromis niloticus in order to understand the intersex relationship of fish, in which sequential hermaphrodism can consist of a male changing into a female (protandry) or a female changing into a male (protogyny). The fish were equally divided into 3 groups. The first group was the control group;the second and third groups were treated with 10 and 100 mg L-1 of 17β-estradiol, respectively, for 30 days. The overall result in this experiment had no significant effect on the growth parameters. Among the two treated groups, the low concentration group shows results similar to those of the control groups. The high concentration group shows changes to the male reproductive system with the appearance of the testis-ova present resulting in an intersex condition of the male gonads. With this experiment, it can be concluded that 17β-estradiol at high concentration reveals positive changes towards the male reproductive system of the fish, Oreochromis niloticus.

关键词： Oreochromis niloticus Nile Tilapia Endocrine Disruption Xenoestrogens Testis-Ova 17β-estradiol

Impact of 17β-estradiol on Natural Water’s Heterotrophic Nitrifying Bacteria

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Journal of Geoscience and Environment Protection 2020年第10期8卷 230-241页

作者： Ziyi Dong Changhao Xiao Weihua Zeng Jinbo Zhao State Key Laboratory of Environment Simulation and Pollution Control School of Environment Beijing Normal University Beijing China

In this research, bottom water samples were collected from nature water. After cultivating and selecting, bacteria which could use (NH4)2SO4 as the only nitrogen source had been selected. The bacteria were cultivated in BM cultures with 0, 0.1, 1, 10, 100 ng/L 17β-estradiol (E2), and the initial concentration of E2 is the only difference between cultures of each group. BM culture is a kind of bacteria culture with 100 mg/L of NH4-N as only nitrogen source. Every group’s N- NH4+, N- NO3-concentration and OD600 were measured. The result shows that compared with the control group, in which no E2 was added, the growth of heterotrophic nitrifying bacteria had been promoted when the concentration of E2 was in range of 1 - 100 ng/L. In addition, heterotrophic nitrifying bacteria’s growing speed has a positive correlation between the E2’s concentration. However, low concentration of E2 (like 0.1 ng/L), could inhibit the growth of heterotrophic nitrifying bacteria. Considering the impact of E2 on heterotrophic nitrifying bacteria, it is necessary to intensify the detection of E2 in the future.

关键词： 17β-estradiol Nitration Heterotrophic Nitrifying Bacteria