Recovery of gonadal development in tiger puffer Takifugu rubripes after exposure to 17β-estradiol during early life stages

作     者：胡鹏 刘滨 孟振 刘新富 贾玉东 杨志 雷霁霖 

作者机构：Key Laboratory of Mariculture of Ministry of EducationOcean University of ChinaQingdao 266003China Municipal Key Laboratory of Marine Fish Breeding and BiotechnologyYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Tianyuan Aquaculture Co.Ltd.of Yantai Economic Development ZoneYantai 264000China 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2017年第35卷第3期

页      面：613-623页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the China Agriculture Research System(No.CARS-50-G20) the National Natural Science Foundation of China(No.31402284) the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A413-2) 

主　　题：Takifugu rubripes 17β-estradiol sex reversal gonadal structure morphological recovery 

摘      要：The aim of the present study was to investigate the long-term effects of 17l）-estradiol （E2） exposure on gonadal development in the tiger puffer （Taktfugu rubripes）, which has a genetic sex determination system of male homogametic XY-XX. Tiger puffer larvae were exposed to 1, 10 and 100 μg/L E2 from 15 to 100 days post-hatch （dph） and then maintained in clean seawater until 400 dph. Changes in sex ratio, gonadal structure and gonadosomatic index （GSI） were monitored at 100, 160, 270 and 400 dph. Sex-associated single nucleotide polymorphism （SNP） markers were used to analyze the genetic sex of samples, except those at 100 dph. Exposure had a positive effect on the conversion of genetically male gonads into phenotypically female gonads at 100 dph. However, gonads from 60% of genetic XY males in the 1-μg/L E2 group and 100% in the 10-μg/L E2 group developed intersexual gonads at 160 dph; gonads of all genetic XY males in the two treatment groups reverted to testis by 270 dph. While 38%, 57% and 44% of gonads of XY fish in the 100-gg/L E2 group reverted to intersexual gonads at 160, 270 and 400 dph, respectively, none reverted to testis after E2 treatment. In addition, E2 exposure inhibited gonadal growth of both genetic sexes, as indicated by the clear dose-dependent decrease in GSI at 270 and 400 dph. The results showed that exposure to E2 during the early life stages of tiger puffer disrupted gonadal development, but that fish recovered after migration to clean seawater. The study suggests the potential use of tiger puffer as a valuable indicator species to evaluate the effects of environmental estrogens on marine fish, thereby protecting valuable fishery resources.