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绿谷灵芝抑制低密度脂蛋白的氧化及单核细胞对内皮细胞的粘附作用

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中国中西医结合杂志 2002年第7期22卷 534-537页

作者：张红梅田辉凯林志彬李学军姚伟娟北京大学医学部药理系北京100083 北京大学医学部血液流变学研究中心

目的:采用血清药理学方法研究绿谷灵芝对低密度脂蛋白（LDL）氧化作用的影响,以及对氧化型低密度脂蛋白（ox-LDL）和晚期糖基化终末产物（AGE）诱导的单核细胞内皮细胞粘附作用的影响。方法:通过检测培养上清中硫代巴比妥酸反应物质（TB... 详细信息

目的:采用血清药理学方法研究绿谷灵芝对低密度脂蛋白（LDL）氧化作用的影响,以及对氧化型低密度脂蛋白（ox-LDL）和晚期糖基化终末产物（AGE）诱导的单核细胞内皮细胞粘附作用的影响。方法:通过检测培养上清中硫代巴比妥酸反应物质（TBARS）的生成表示含药血清对LDL的氧化作用,用白细胞髓过氧化物酶法测定单核细胞对内皮细胞的粘附作用。结果:0.12g/kg绿谷灵芝一次给药组0.5h、1h、2h、3h,两次给药0.5h、1h取血分离得到的血清对LDL氧化作用无明显影响,两次给药2h、3h以及0.12g/kg、0.24g/kg、0.72g/kg绿谷灵芝连续给药10天后,取血分离得到的血清均可降低LDL的氧化作用（P细胞对内皮细胞的粘附作用（P作用,减轻由ox-LDL和AGE诱导的单核细胞对内皮细胞的粘附作用。

关键词：绿谷灵芝血清药理学低密度脂蛋白晚期糖基化终末产物内皮细胞单核细胞细胞粘附作用

接种浓度对人骨髓基质干细胞在脱钙骨上生长和分布的影响

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上海第二医科大学学报 2004年第4期24卷 232-234页

作者：李东柳向东肖开颜舒朝锋崔磊刘伟曹谊林上海第二医科大学第九人民医院整复外科上海市组织工程研究与开发中心上海200235

目的研究不同接种浓度对人骨髓基质干细胞在脱钙骨支架上生长过程及分布的影响。方法荧光活性染料DiI标记人骨髓基质干细胞,接种于部分脱钙骨支架材料上,测定细胞的粘附率;将细胞悬液浓度分别调整为20×106、10×106、5×106/mL和空白... 详细信息

目的研究不同接种浓度对人骨髓基质干细胞在脱钙骨支架上生长过程及分布的影响。方法荧光活性染料DiI标记人骨髓基质干细胞,接种于部分脱钙骨支架材料上,测定细胞的粘附率;将细胞悬液浓度分别调整为20×106、10×106、5×106/mL和空白组,依次接种于同一块脱钙骨支架4个相邻象限的中心位置上。接种后第2、7 d在倒置荧光显微镜下观察材料表面的细胞分布、生长的情况,测量细胞材料面积比。结果 DiI标记的骨髓基质干细胞的粘附率为(99.9±0.2)％,未标记的骨髓基质干细胞粘附率为(99.1±1)％,两者无统计学差异(P>0.05)。不同浓度细胞的细胞材料面积比无差异(P>0.05)。结论通过DiI标记可以动态观察细胞在材料上的生长状况。20×106、10×106、5×106/mL接种浓度均能达到覆盖脱钙骨表面直接接种部位的目的。

关键词：组织工程骨髓基质干细胞细胞支架脱钙骨细胞粘附作用

Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials

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Chinese Journal of Traumatology 2003年第4期6卷 209-212页

作者：王光林林卫杨志明裴福兴刘雷 Department of Orthopaedics the West China Hospital Sichuan UniversityChengdu 610041China Department of Obstetrics and Gynecology the West China HospitalSichuan UniversityChengdu 610041China Department of Orthopaedics the West China Hospital Sichuan UniversityChengdu 610041Chinabjective: To study the adhesion migration and three-dimentional growth of Schwann cells on PLA (polylactic acid) nonspinning fibre cloth and polyglycolic/ polylactic acid (PLGA) fibres.Methods: Schwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal microscope.Results: Schwann cell/ECM solution wascompounded with PLA nonspinning fibre cloth. With formation of gel most Schwann cells resided in the fibre net holes and adhered to the fibres to form a multiplayer-arranged Schwann cell column like Bungner band. Schwann cells could adhere to PLGA fibres and grew and migrated along the fibres. ECM gel could significantly increase the adhering and migrating cell number.Conclusions: ECM gel can facilitate the adhesion growth and migration of Schwann cells on the seteroframe. It is a good integrating material for tissue engineering bioartificial nerve.

Objective: To study the adhesion, migration and three-dimentional growth of Schwann cells on PLA (polylactic acid) nonspinning fibre cloth and polyglycolic/polylactic acid (PLGA) ***: Schwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen, polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal ***: ECM gel can facilitate the adhesion, growth and migration of Schwann cells on the seteroframe. It is a good integrating material for tissue engineering bioartificial nerve.

关键词：许旺氏细胞生物材料组织相容性细胞粘附作用细胞培养

E—cadherin、α—catenin和β—catenin的表达与食管鳞癌发生的免疫组化研究

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胃肠病学 2001年第C00期6卷 8-8页

作者：李继昌王宛明河南医科大学第一附属医院消化内科450052

Effects of cg,t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901

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World Journal of Gastroenterology 2004年第10期10卷 1392-1396页

作者： Bing-QingChen Yan-MeiYang QiWang Yan-HuiGao Jia-RenLiu Jing-ShuZhang Xuan-LinWang Rui-HaiLiu DepartmentofNutritionandFoodHygiene PublicHealthCollegeHarbinMedicalUniversityHarbin150001HeilongjiangProvinceChina MedicalCollegeofShantouUniversity Shantou515031GuangdongProvinceChina ChineseCenterforDiseaseControlandPrevention theCenterforEndemicDiseaseControlBeijing.China FoodScienceandToxicology DepartmentofFoodScience108StockingHallCornellUniversityIthacaNY14853-7201USA

AIM: To investigate the effect of c9, t11-conjugated linoleic acid (c9,t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901).METHODS: SGC-7901 cells were at first treated with different concentrations (25, 50, 100, 200 μmol/L) of c9,t111-CLA and 1 mL/L ethanol (as a negative control) for 24 *** adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD), α-catenin,intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these ***: The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9,t11-CLA (0,25, 50, 100, and 200 μmol/L) was 100.0±3.3, 95.7±4.0,89.2±4.6, 87.9±6.1, and 65.9±5.8, respectively. The attachment rate to fibronectin was 100.0±4.7, 96.8±3.8,94.5±4.1, 76.5±4.3, and 61.8±4.8, respectively. The attachment rate to Matrigel was 99.9±6.6, 91.4±6.8,85.5±7.4, 79.3±5.6, and 69.6±5.1, respectively. Besides,c9,t11-CLA could increase the level of ECD and α-catenin,and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 ***: c9, t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin, fibronectin and Matrigel. c9,t11-CLA can increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in human gastric carcinoma cells.

关键词：胃癌细胞培养亚油酸 SGC-7901细胞系细胞粘附作用