Experimental study on the adhesion, migration and three-dimensional growth of Schwann cells on absorbable biological materials

作     者：王光林 林卫 杨志明 裴福兴 刘雷 

作者机构：Department of Orthopaedicsthe West China Hospital Sichuan UniversityChengdu 610041China Department of Obstetrics and Gynecologythe West China HospitalSichuan UniversityChengdu 610041China Department of Orthopaedicsthe West China Hospital Sichuan UniversityChengdu 610041Chinabjective: To study the adhesion migration and three-dimentional growth of Schwann cells on PLA (polylactic acid) nonspinning fibre cloth and polyglycolic/ polylactic acid (PLGA) fibres.Methods: Schwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal microscope.Results: Schwann cell/ECM solution wascompounded with PLA nonspinning fibre cloth. With formation of gel most Schwann cells resided in the fibre net holes and adhered to the fibres to form a multiplayer-arranged Schwann cell column like Bungner band. Schwann cells could adhere to PLGA fibres and grew and migrated along the fibres. ECM gel could significantly increase the adhering and migrating cell number.Conclusions: ECM gel can facilitate the adhesion growth and migration of Schwann cells on the seteroframe. It is a good integrating material for tissue engineering bioartificial nerve. 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2003年第6卷第4期

页      面：209-212页

学科分类：0403[教育学-体育学] 1002[医学-临床医学] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：This study was supported by the National Natural Science Foundation of China(No.30100190) 

主　　题：许旺氏细胞 生物材料 组织相容性 细胞粘附作用 细胞培养 

摘      要：Objective: To study the adhesion, migration and three-dimentional growth of Schwann cells on PLA (polylactic acid) nonspinning fibre cloth and polyglycolic/polylactic acid (PLGA) ***: Schwann cells/ECM gel solution and PLA nonspinning fibre cloth and PLGA fibres pretreated by collagen, polylysine and ECM were co-cultured. Then the migration and three-dimensional growth of Schwann cells on the fibres were observed under phase contrast microscope and laser scanning confocal ***: ECM gel can facilitate the adhesion, growth and migration of Schwann cells on the seteroframe. It is a good integrating material for tissue engineering bioartificial nerve.