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Antioxidant xanthones and anthraquinones isolated from a marine-derived fungus Aspergillus versicolor

Chinese Journal of Natural Medicines 2018年第3期16卷 219-224页

作者： WU Ze-Hong LIU Dong XU Ying CHEN Jian-Liang LIN Wen-Han The Eighth Affiliated Hospital Sun Yat-sen University State Key Laboratory of Natural and Biomimetic Drugs Peking University Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science Shenzhen Engineering Laboratory for Marine Algal Biotechnology College of Life Science Shenzhen University

Chemical examination of an EtO Ac extract of cultured Aspergillus versicolor fungus from deep-sea sediments resulted in the isolation of four xanthones, eight anthraquinones and five alkaloids, including a new xanthone, oxisterigmatocystin D(1) and a new alkaloid, aspergillusine A(13). High resolution electron impact mass spectrometry(HR-EI-MS), FT-IR spectroscopy, and NMR techniques were used to elucidate the structures of these compounds, and the absolute configuration of compound 1 was established by its NMR features and coupling constant. Furthermore, the biosynthesis pathway of these xanthones and anthraquinones were deduced, and their antioxidant activity and cytotoxicity in human cancer cell lines(HTC-8, Bel-7420, BGC-823, A549, and A2780) were evaluated. The trolox equivalent antioxidant capacity(TEAC) assay indicated most of the xanthones and anthraquinones possessing moderate antioxidant activities. The Nrf2-dependent luciferase reporter gene assay revealed that compounds 6, 7, 9, and 12 potentially activated the expression of Nrf2-regulated gene. In addition, compounds 5 and 11 showed weak cytotoxicity on A549 with the IC50 values of 25.97 and 25.60 μmol×L^(–1), respectively.

关键词：曲霉属菌多色氧杂蒽酮 Anthraquinones 抗氧化剂活动 Cytotoxicity

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Effects of ferulic acid on antioxidant activity in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and their combination

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Chinese Journal of Natural Medicines 2015年第6期13卷 401-408页

作者： WANG Lin-Yan TANG Yu-Ping LIU Xin ZHU Min TAO Wei-Wei LI Wei-Xia DUAN Jin-Ao Jingjiang Hospital of Traditional Chinese Medicine Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine Nanjing University of Chinese Medicine

The present study aimed at exploring different roles of the same compound in different environment, using preparative HPLC, and the significance to investigating bio-active constituents in traditional Chinese medicine(TCM) on the basis of holism. In this study, the depletion of target component ferulic acid(FA) by using preparative HPLC followed by antioxidant activity testing was applied to investigate the roles of FA in Angelicae Sinensis Radix(DG), Chuanxiong Rhizoma(CX) and their combination(GX). The antioxidant activity was performed by 1, 1-diphenyl-2-picrylhydrazyl(DPPH) radical-scavenging activity testing. FA was successfully and exclusively depleted from DG, CX, and GX, respectively. By comparing the effects of the samples, it was found that FA was one of the main antioxidant constituents in DG, CX and GX, and the roles of FA were DG > CX > GX. Furthermore, the effects of FA varied at different doses in these herbs. This study provided a reliable and effective approach to clarifying the contribution of same compound in different TCMs to their bio-activities. The role of a constituent in different TCMs might be different, and a component with the same content might have different effects in different chemical environments. Furthermore, this study also suggested the potential utilization of preparative HPLC in the characterization of the roles of multi-ingredients in TCM.

关键词： HPLC Ferulic 酸当归 sinensis Ligusticum chuanxiong 植物对抗氧化剂活动

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Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica

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Chinese Journal of Natural Medicines 2015年第6期13卷 445-453页

作者： HU Wen GUO Ting JIANG Wen-Jun DONG Guang-Li CHEN Da-Wei YANG Shi-Lin LI He-Ran College of Pharmacy Soochow University Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application Department of Polymer Science and Engineering College of Chemistry Chemical Engineering and Materials Science Soochow University Guiding Long Da Honeysuckle Technology Co. Ltd.

The present study was designed to establish and optimize a new method for extracting chlorogenic acid and cynaroside from Lonicera japonica Thunb. through orthogonal experimental designl. A new ultrahigh pressure extraction(UPE) technology was applied to extract chlorogenic acid and cynaroside from L. japonica. The influential factors, including solvent type, ethanol concentration, extraction pressure, time, and temperature, and the solid/liquid ratio, have been studied to optimize the extraction process. The optimal conditions for the UPE were developed by quantitative analysis of the extraction products by HPLC-DAD in comparison with standard samples. In addition, the microstructures of the medicinal materials before and after extraction were studied by scanning electron microscopy(SEM). Furthermore, the extraction efficiency of different extraction methods and the 2, 2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activities of the extracts were investigated. The optimal conditions for extracting chlorogenic acid and cynaroside were as follows: ethanol concentration, 60%; extraction pressure, 400 MPa; extraction time, 2 min; extraction temperature, 30 °C; and the solid/liquid ratio, 1 : 50. Under these conditions, the yields of chlorogenic acid and cynaroside were raised to 4.863% and 0.080%, respectively. Compared with other extraction methods, such as heat reflux extraction(HRE), ultrasonic extraction(UE), and Sohxlet extraction(SE), the UPE method showed several advantages, including higher extraction yield, shorter extraction time, lower energy consumption, and higher purity of the extracts. This study could help better utilize L. japonica flower buds as a readily accessible source of natural antioxidants in food and pharmaceutical industries.

关键词：忍冬装饰用的梨树 Thunb 超离频压力抽取 Chlorogenic 酸 Cynaroside 抗氧化剂活动

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Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus

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Asian Pacific Journal of Tropical Medicine 2017年第8期10卷 855-862页

作者： Hafiza Noreen Nabil Semmar Muhammad Farman James S.O.McCullagh Department of Chemistry Quaid-i-Azam UniversityIslamabad 45320Pakistan Institut Supérieur des Sciences Biologiques Appliquées de Tunis Tunisia Chemistry Research Laboratory Department of ChemistryUniversity of OxfordMansfield RoadOxford OX1 3TAUnited Kingdom

Objective: To evaluate the total phenolic content and compare the antioxidant activity of various solvent extracts and fractions from the aerial parts of Coronopus didymus through various assays. Methods: Total phenolic content was determined using the Folin-Ciocalteu assay and the in vitro antioxidant activity of a number of different extracts was investigated in a dose-dependent manner with three different methods: the 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) and ferric reducing antioxidant power(FRAP) assays. A flavone was isolated from the most active ethanolic extract with high antioxidant activity using size exclusion chromatography. IC_(50) values were calculated for the DPPH and ABTS methods. The FRAP activity was assessed in terms of μM Fe(II) equivalent. Results: The phenolic content was found to be highest in the ethanol extract(CDA Et; 47.8 mM GAE) and the lowest in the dichloromethane extract(CDA DCM; 3.13 mM GAE). The ethanol extract showed high radical scavenging activity towards DPPH and ABTS radicals with IC_(50) values of(7.80 × 10~2) and(4.32 × 10~2) μg/m L, respectively. The most active ethanol extract had a FRAP value of 1 921.7 μM Fe(Ⅱ) equivalent. The isolated flavone F10C(5,7,4'-trihydroxy-3'-methoxy flavone) was far more effective for scavenging free radicals in the DPPH and ABTS assays with IC_(50) of 43.8 and 0.08 μg/m L, than the standard trolox, with IC_(50) values of 97.5 and 21.1 μg/m L, respectively. In addition, the flavone F10C and the standard ascorbic acid had FRAP values of 1 621.7 and 16 038.0 μM Fe(Ⅱ) equivalents, respectively. Conclusions: The total phenolic content of extracts in decreasing order is ethanol extract(CDA Et)> acetone extract(CDA ACE)> phenolic extract(CDA MW)> n-hexane extract(CDA nHX)> chloroform extract(CDA CHL)> dichloromethane extract(CDA DCM). The ordering of extracts in terms of antioxidant activity from highest to lowest is CDA Et> CDA MW> CDA DCM> CDA CHL> CDA ACE> CDA nHX in DPP

关键词： Coronopus didymus 抗氧化剂活动总计酉分的内容缩放排除层析

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Antioxidant antileishmanial cytotoxic and antimicrobial activities of a local plant Myrtus nivellei from Algeria Sahara

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Asian Pacific Journal of Tropical Biomedicine 2017年第8期7卷 702-707页

作者： Farah Ramdane Rym Essid Nadia Fares Dahmane El Ouassis Sana Aziz Mahfoud Hadj Mahammed Mohamed Didi Ould Hadj Ferid Limam Biogeochemistry Laboratory in Desert Environments Kasdi Merbah University Faculty of Natural and Life Sciences El Oued University Laboratory of Bioactive Substances Biotechnology Center of Borj-Cedria Technopole National Institute of Forest Research Protection Laboratory of Ecosystem in Arid and Semi Arid Zone Kasdi Merbah University

Objective: To study the phytochemical constituents and in vitro biological activities of hydromethanolic extract and fractions from Algerian Sahara Myrtus nivellei(M. nivellei)collected in Hoggar region and to identify the active fraction that can act as an alternative of commonly used antibiotics and as antileishmanial or antioxidant agents.Methods: Phytochemical screening of M. nivellei aerial parts was realised according to the literature. Extract was firstly prepared by using aqueous methanol then fractionated with ethyl acetate and butanol solvents. Total phenolics, tannis and flavonoids, of the hydromethanolic extract and their fractions were determined by Folin-Ciocalteu method as gallic acid equivalents and by aluminium chloride as rutin equivalent respectively.Extract and fractions were tested for their antimicrobial and antiparasital activities against standard bacteria using agar diffusion method and two kinds of leishmania visceral and cutaneous. The antioxidant activities were realized using phosphomolybdenum, FRAP and DPPH tests.Results: Preliminary phytochemical screening exhibited the presence of flavonoids,tannins, saponins, and alkaloids. The experimental results showed that plant extract and fractions were high in phenolic compounds and exhibited an important role as antioxidant,antimicrobial and had a moderate antileishmanial activity.Conclusions: These observations lead us toward more studies in this field, so that we can get more benefits from our local Algerian medicinal plants.

关键词：酉分的混合物 Flavonoids 抗氧化剂活动抗菌剂活动 Antileishmanial 活动 Myrtus nivellei

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Antihypertensive and antioxidant activity of Cassytha filiformis L.:A correlative study

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 614-618页

作者： yori yuliandra a.armenia helmi arifin Faculty of Pharmacy Andalas University Padang West Sumatra Indonesia

Objective: To study the blood pressure lowering effect of Cassytha filiformis extract in animal models of hypertension and its correlation with the antioxidant activity.Methods: Male Sprague–Dawley rats were divided into two groups: endocrine hypertension(HTN group) that received a combination of prednisone and salt for two weeks and oxidative stress-associated hypertension(HTN-OS group) that received additional induction of L-Nitro Arginine Methyl Esther(L-NAME) for two days. Each group was subdivided into 4 and treated intravenously with the extract 5; 10; and 20 mg/kg, and vehicle control. The systolic blood pressure(SBP), diastolic blood pressure(DBP), and mean arterial pressure(MAP) were recorded. The blood was taken before and at the end of recording for the measurement of serum concentration of nitric oxide(NO). The changes of blood pressure were analyzed by two-way ANOVA while its correlation with NO concentration was analyzed by Pearson's Correlation.Results: The study showed a significant antihypertensive effect of the extract as compared with control group(P 0.05).Conclusions: The study concludes that C. filiformis extract in the dose of 5 mg/kg exhibits the best blood pressure lowering effect in both animal models. Antihypertensive activity of the extract is not correlated with its antioxidant effect.

关键词： Cassytha filiformis 血压氧化应力抗氧化剂活动氮的氧化物关联

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