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在吸取卵泡时测量卵巢内动脉的搏动指数和阻力指数评价卵子质量的有用性

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世界核心医学期刊文摘（妇产科学分册） 2006年第6期2卷 15-16页

作者： Takahashi K. Ishida G.M. 张旸 Department of Obstetrics and Gynecology Yamagata University School of Medicine 2-2-2 Iidanishi Yamagata 990- 9585 JapanDr.

Objective: To examine the correlation between the pulsatility and resistance indices (PI and RI) of the intraovarian artery on the day of follicle aspiration and the oocyte quality, intrafollicular oxidative stress, and luteinization. Pulsatility index and RI on the day of hCG administration also were measured. Design: Prospective study. Setting: Obstetrics and gynecology department of a university medical school in Japan. Patient(s): Thirty- five patients in an IVF- ET program. Intervention(s): The PI and RI of the intraovarian artery were measured by transvaginal color ultrasonographic pulsed wave Doppler on the day of hCG administration and the day of follicle aspiration. Follicular fluids and the granulosa cells were collected at follicle aspiration. Main Outcome Measure(s): The PI and RI of the intraovarian artery blood flowon the day of hCG administration and of follicle aspiration, as well as the rate of development of mature oocytes, follicular fluid steroid levels, and the incidence of apoptosis in the granulosa cells. Result(s): The PI and RI on the day of follicle aspiration were correlated positively with the rate of mature oocytes retrieved (PI: r = 0.429; RI: r = 0.348), were correlated negatively with the incidence of apoptotic mural (PI: r =- 0.383; RI: r =- 0.459) and cumulus (PI: r = - 0.378; RI: r =- 0.469) granulosa cells, and were negatively correlated with the concentration of P in the follicular fluid (PI: r =- 0.429; RI: r =- 0.359). The PI and RI on the day of hCG administration were negatively correlated only with the total number of retrieved oocytes (PI: r =- 0.393; RI: r =- 0.374). Conclusion(s): The PI and RI of the intraovarian artery blood flow measured on the day of follicle aspiration may be good indicators of the follicle luteinization and oxidation as well as of oocyte quality.

关键词：阻力指数(RI) 动脉搏动指数卵子质量卵泡内指数评价测量颗粒细胞凋亡人绒膜毛促性腺激素

体外培养人卵巢黄素化颗粒细胞的鉴定及其分泌功能变化

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中华妇产科杂志 2005年第5期40卷 351-352页

作者：白晓红糜若然岳天孚随笑琳赵晓徽天津医科大学总医院妇产科 300052

人卵巢黄素化颗粒细胞(颗粒细胞)是卵泡内主要的体细胞成分,它介导促性腺激素对卵母细胞成熟的调节,通过自分泌或旁分泌作用,维持有利于卵母细胞成熟的微环境.因此,研究颗粒细胞的功能和调控,对于了解卵母细胞的成熟规律有重要意义.本... 详细信息

人卵巢黄素化颗粒细胞(颗粒细胞)是卵泡内主要的体细胞成分,它介导促性腺激素对卵母细胞成熟的调节,通过自分泌或旁分泌作用,维持有利于卵母细胞成熟的微环境.因此,研究颗粒细胞的功能和调控,对于了解卵母细胞的成熟规律有重要意义.本研究的目的是探讨颗粒细胞的分泌功能,及其与胰岛素样生长因子Ⅰ(IGF-Ⅰ)及表皮生长因子(EGF)的关系。

关键词：卵巢黄素化颗粒细胞胰岛素样生长因子Ⅰ(IGF-Ⅰ) 功能变化体外培养表皮生长因子(EGF) 卵母细胞成熟鉴定促性腺激素细胞成分分泌作用重要意义分泌功能卵泡内自分泌微环境

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宫腔内人工授精

济宁医学院学报 2007年第3期30卷 259-261页

作者：唐乃富李秀英济宁医学院附属第一人民医院济宁医学院妇产科学教研室

人工授精是指通过人工方法用器皿将处理后的精液注入阴道及子宫颈附近的方式使妇女受孕,分为夫精人工授精(artificial insemination by husband,AIH)及供精人工授精(artificial insemination by donor,AID)。根据注入途径的不同分为阴... 详细信息

人工授精是指通过人工方法用器皿将处理后的精液注入阴道及子宫颈附近的方式使妇女受孕,分为夫精人工授精(artificial insemination by husband,AIH)及供精人工授精(artificial insemination by donor,AID)。根据注入途径的不同分为阴道内人工授精、宫颈人工授精、宫腔内人工授精、直接卵泡内授精、经阴道输卵管内受精、腹腔镜下精子输卵管内移植。[第一段]

关键词：宫腔内人工授精输卵管内移植夫精人工授精供精人工授精阴道内人工方法子宫颈卵泡内

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同一次级卵泡中见两个卵母细胞一例

四川解剖学杂志 2015年第2期23卷 F0003-F0003页

作者：王健君李晓敏皖南医学院组织学与胚胎学教研室芜湖241002

组织学与胚胎学实验课教学中观察女性生殖系统器官卵巢的石蜡切片，HE染色。发现同一次级卵泡中虬两个卵母细胞一例（见图1）。卵巢皮质中有可发育及成熟的卵泡，卵泡呈球形，南一个卵母细胞和包嗣在其周同的多个卵泡细胞组成。而通过... 详细信息

组织学与胚胎学实验课教学中观察女性生殖系统器官卵巢的石蜡切片，HE染色。发现同一次级卵泡中虬两个卵母细胞一例（见图1）。卵巢皮质中有可发育及成熟的卵泡，卵泡呈球形，南一个卵母细胞和包嗣在其周同的多个卵泡细胞组成。而通过观察实验教学用切片，发现其中一个次级卵泡内存在两个初级卵母细胞，每个初级卵母细胞周嗣均可见透明带及放射冠，分别突人卵泡腔后形成两个卵丘，

关键词：初级卵母细胞卵泡内石蜡切片卵巢皮质实验课教学系统器官女性生殖 HE染色