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世界核心医学期刊文摘（妇产科学分册） 2006年第5期2卷 52-53页

作者： Yakirevich E. Sabo E. Naroditsky I. 李奎 Department of Pathology Rhode Island Hospital APC 12 593 Eddy St. Providence RI 02903 United States

Multidrug resistance (MDR) to chemotherapy is a major obstacle in attempts to improve the clinical outcome of ovarian carcinoma patients. The MDR-related phenotype is associated with over-expression of certain drug transporters, such as P-glycoprotein (P- gp), multidrug resistance protein 1 (MRP1), and lung resistance protein (LRP). The aim of this study was to evaluate the extent and prognostic significance of MDR-related protein expression in ovarian serous carcinomas. In addition, we correlated expression of these proteins with the apoptosis-related proteins p53, bcl- 2, and bax. Methods. Consecutive sections from 60 cases of ovarian serous carcinoma were assessed immunohistochemically for expression of P- gp, MRP1, LRP, p53, bcl- 2, and bax. The level of protein expression was scored based on staining intensity and extent. Results. Strong P-gp expression was observed in 12 (20% ), MRP1 in 39 (65% ), LRP in 27 (45% ), p53 in 45 (75% ), bcl- 2 in 25 (41.7% ), and bax in 30 (50% ) of the 60 tumors. MRP1 expression was associated with both p53 and bcl- 2 expressions (P = 0.01 and P = 0.03, respectively). Univariate analysis of survival revealed a significant inverse correlation between P- gp expression and patient survival (P = 0.015). Moreover, P- gp expression was significantly increased in tumors of patients unresponsive to chemotherapy (P = 0.009). Multivariate analysis revealed that only FIGO stage and P- gp expression were useful negative independent predictors of survival (P = 0.035 and P = 0.045, respectively). Conclusions. Our pilot study demonstrates that P- gp expression may be a reliable independent prognostic factor of survival in patients with ovarian serous carcinoma. Moreover, P- gp immunostaining may be useful for dividing ovarian carcinoma patients into chemoresponsive and chemoresistant groups.

关键词：凋亡相关蛋白化疗耐药多药耐药蛋白免疫组化法临床结局染色强度连续切片预后因子免疫染色

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转移抑制基因nm23蛋白表达的升高与上皮性卵巢癌患者无进展生存期缩短有关

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国际妇产科学杂志 1997年第1期24卷 58-59页

作者：刘萍

用免疫组织化学方法对nm23基因与上皮性卵巢癌(EOC)关系进行两方面探讨:①在EOC中nm23-H1/NDPK的表达类型和强度,②在EOC中nm23-H1表达的强度和类型与组织学分级,FIGO分期,治疗反应之间的关系以确定无进展生存率和总体生存率。试图测定... 详细信息

用免疫组织化学方法对nm23基因与上皮性卵巢癌(EOC)关系进行两方面探讨:①在EOC中nm23-H1/NDPK的表达类型和强度,②在EOC中nm23-H1表达的强度和类型与组织学分级,FIGO分期,治疗反应之间的关系以确定无进展生存率和总体生存率。试图测定EOC中nm23表达,以确定其能否作为预测肿瘤转移潜力和生存率的标记物。

关键词：上皮性卵巢癌免疫染色无进展生存组织学分级 nm23 患者

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用多聚酶链反应和免疫组织化学方法评估HHV-8/KSHV在卡波济肉瘤发生中的作用

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世界核心医学期刊文摘（皮肤病学分册） 2005年第6期1卷 28-29页

作者： Pak F. Pyakural P. Kokhaei P. 冯义国 Immunopathology Laboratory Karolinska Hospital 171-76 Solna Stockholm Sweden

The human γ-herpes virus- 8 (HHV-8) was first described in AIDS- related Kaposi’ s sarcoma (KS) tumour samples. In this study, we report comparative studies on paraffin-embedded biopsies of AIDS-related KS (AKS) and endemic KS (EKS)with regard to HHV-8 content as evaluated using polymerase chain reaction (PCR) and immunohistochemistry. DNA was extracted either using Chelex-100 or using Qia- gene kit and was evaluated with the help of a semiquantitative PCR assay. The PCR detection of HHV- 8 was more sensitive to the Chelex method than to Qia- gene. The threshold for PCR test sensitivity with the help of serial dilution of DNA was at the level of five plasmid ORF-26 regions, and DNA from 25 body cavity-based lymphoma- 1 cells. The results expressed as virus load/actin unit showed progressively higher HHV-8 levels in late (nodular) cases, compared to those in early (patch/plaque)stages. Evaluation of HHV- 8 DNA levels in tumour tissues, thus, indicates a correlation between virus load and KS stage. Double immunostaining of spindle cells(SC)inKSbiopsiesfor CD34 and HHV-8/latency- associated nuclear antigen (LANA)showed an increase in double- positive SC in the lesions of nodular AKS and EKS cases, compared to that in plaque and- patch stages. However, 10- 15% of CD34+ /LANA SC cells were observed during the development from patch to nodular casesofAKSand EKS.Our results indicate that PCR analysis is a simple and sensitive diagnostic method for HHV-8 evaluation in KS tissues, processed for conventional histopathology.

关键词：多聚酶链反应卡波 HHV-8/KSHV 免疫组织化学聚合酶链反应免疫染色淋巴瘤组织病理学核抗原 DNA

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自身免疫性胰腺炎中发现的皮质激素反应性胰腺囊肿

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世界核心医学期刊文摘（胃肠病学分册） 2006年第2期2卷 29-30页

作者： Muraki T. Hamano H. Ochi Y. S. Kawa 赵萌 Department of Medicine Gastroenterology Shinshu University School of Medicine 3- 1- 1 Asahi Matsumoto 390- 8621 Japan

There have been only a few reports of autoimmune pancreatitis complicated with pancreatic cyst and such cysts are rare, probably due to the absence of severe tissue necrosis and/or lack of stasis of the pancreatic juice in this condition. However, during a follow- up of 48 patients with this disease, we found 3 patients with pancreatic cysts, and this enabled us to evaluate their clinicopathological findings. Between September 1994 and July 2003, we treated and followed 48 patients with autoimmune pancreatitis, and found 3 patients with pancreatic cyst formation that was responsive to corticosteroid therapy. All of the patients with cysts had high serum IgG4 concentrations. After corticosteroid therapy, rapid resolution of the pancreatic cysts was observed. Immunostaining with goat polyclonal antibody for each IgG subclass showed severe infiltration of IgG4- posi- tive plasma cells in the cyst wall in one patient. The high serum IgG4 concentration and favorable response to corticosteroid therapy suggests that a highly active state of the inflammatory process is closely associated with cyst formation, and that a corticosteroid- responsive pancreatic cyst is a characteristic feature of autoimmune pancreatitis.

关键词：胰腺囊肿皮质激素囊肿壁 IgG 免疫染色病理检查活性状态

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1例皮肤Rosai-Dorfman病发生结晶沉积

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世界核心医学期刊文摘（皮肤病学分册） 2005年第12期1卷 29-30页

作者： Motta L. McMenamin M.E. Thomas M.A. Calonje E. 惠海英 Dr. Department of Histopathology St. John' s Institute of Dermatology St. Thomas' Hospital LondonUnited Kingdom

Rosai- Dorfman Disease (RDD) is an idiopathic reactive proliferation of distinctive histiocytes that have abundant cytoplasm and commonly exhibit intracytoplasmic ingestion of inflammatory cells (emperipolesis). The histiocytes are immunopositive for S100 protein and are typically associated with an infiltrate of lymphocytes, plasma cells, and neutrophils. The classic clinical presentation is massive enlargement of (usually) cervical lymph nodes with a histologic appearance that mimics exaggerated sinus histiocytosis. RDD can also involve extranodal sites and skin involvement is common either as part of disseminated disease or as a result of primary disease. We report an exceptional case of cutaneous RDD with crystal deposition in a young male presenting with skin nodules. Skin biopsy showed classic features of cutaneous RDD with the additional feature of conspicuous rhomboidal and needle- shaped crystals within the cytoplasm of many lesional plasma cells, histiocytes, and also in an extracellular location. The plasma cells were polyclonal by light chain immunostaining. Crystal deposition has not been reported to date in RDD and is likely a result of the reactive plasma cell proliferation.

关键词：组织细胞中性粒细胞浸润炎性细胞淋巴结外免疫染色播散性轻链共生现象

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可检出临床前期痒病感染羊的新方法

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畜牧兽医科技信息 2001年第4期 11-12页

作者：百里

O’rourke等研究了以免疫组化法检查第三眼睑(瞬膜)淋巴样组织活检标本对绵羊痒病进行临床前期诊断的性能。该法是以特异单克隆抗体[MAb F89/160.1.5和(或)MAb F99.6.1]检测淋巴样组织中的PrP为基础。用此法检查了42只临床可疑绵羊和17... 详细信息

O’rourke等研究了以免疫组化法检查第三眼睑(瞬膜)淋巴样组织活检标本对绵羊痒病进行临床前期诊断的性能。该法是以特异单克隆抗体[MAb F89/160.1.5和(或)MAb F99.6.1]检测淋巴样组织中的PrP为基础。用此法检查了42只临床可疑绵羊和175只和非痒病绵羊的标本(均经常规脑组织PrP免疫染色或组织病理检查确诊或排除痒病),前者41只阳性、后者174只阴性。加检感染羊群临床正常绵羊41只。第三眼睑检查结果与脑PrP免疫染色结果均为阳性

关键词：痒病淋巴样免疫染色病毒病第三眼睑瞬膜绵羊临床前期

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人类同种异体移植瓣膜中骨调节蛋白的表达

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世界核心医学期刊文摘（心脏病学分册） 2007年第2期3卷 39-40页

作者： Shetty R. Pepin A. Charest A. P. Mathieu 郭俊 Hpital Laval 2725 Chemin Ste-Foy Sainte-Foy Que. G1V 4G5 Canada.Dr.

目的:验证同种异体移植瓣膜(VA)钙化起因于有骨调节蛋白表达的骨化过程的假说。方法:对被移植用于治疗心脏瓣膜功能障碍的15例VA进行研究。将VA与正常主动脉瓣膜(n=20)进行分析比较。结果:所有VA(5例主动脉瓣、10例肺动脉瓣)出现重度钙... 详细信息

目的:验证同种异体移植瓣膜(VA)钙化起因于有骨调节蛋白表达的骨化过程的假说。方法:对被移植用于治疗心脏瓣膜功能障碍的15例VA进行研究。将VA与正常主动脉瓣膜(n=20)进行分析比较。结果:所有VA(5例主动脉瓣、10例肺动脉瓣)出现重度钙化和纤维化。免疫组织化学研究显示基质细胞表达骨特异性转录因子Cbfa-1。钙化区域有骨碱性磷酸酶表达。VA的平滑肌(α-SM)肌动蛋白免疫染色多于正常瓣膜,其中有6例VA瓣膜在钙化性结节附近形成细胞簇。

关键词：同种异体移植钙化性骨碱性磷酸酶基质细胞表达肺动脉瓣特异性转录因子免疫染色免疫组织化学

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热效应在淋巴瘤诊断与研究中的应用

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肿瘤研究与临床 1995年第4期7卷 228-229页

作者：王晋芬王全红焦士兰山西省肿瘤医院

石蜡包埋组织免疫组化染色是淋巴瘤病理诊断与研究的重要手段之一，由于常规组织处理过程造成抗原封闭与结构改变，使一些抗体不能用于石蜡切片或反应微弱，我们应用热介导抗原恢复效应引起蛋白结构改变原理，对组织切片在高压锅内进行... 详细信息

石蜡包埋组织免疫组化染色是淋巴瘤病理诊断与研究的重要手段之一，由于常规组织处理过程造成抗原封闭与结构改变，使一些抗体不能用于石蜡切片或反应微弱，我们应用热介导抗原恢复效应引起蛋白结构改变原理，对组织切片在高压锅内进行热处理，使原来只用于冰冻切片的某些抗体现可用于石蜡切片，扩大了抗体的使用范围，提高了免疫组化在淋巴瘤诊断与研究中的应用价值，增强了免疫染色的敏感性，使抗体稀释度增加３～２０倍，使多数淋巴瘤得到准确诊断。

关键词：诊断淋巴瘤抗体石蜡切片抗原冰冻切片组化染色蛋白结构免疫染色稀释度

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宫体小细胞癌可致视觉异常

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世界核心医学期刊文摘（妇产科学分册） 2006年第3期2卷 46-47页

作者： In A.P. 杨晓梅

Background. Small cell carcinoma in the uterine corpus rather than the endometrium has not been reported yet which resembles a uterine leiomyosarcoma on an imaging study. Furthermore, gynecologic malignancies manifested as a visual dysfunction render the early suspicion and accurate diagnosis a difficult proposition. Case. A 57-year-old Korean woman was referred to the Department of Obstetrics and Gynecology due to incidentally detected uterine mass. She had been suffering from a visual loss for several weeks. This uterine mass mimicking a leiomyosarcoma on pelvis MRI was identified to be a small cell carcinoma via a transvaginal gun biopsy and consequent immunostaining. The patient was then scheduled to undergo a regimen of chemotherapy with etoposide and carboplatin. Conclusion. Small cell carcinoma may arise from the uterine corpus rather than the endometrium and may also be accompanied by visual symptoms.

关键词：小细胞癌视觉异常子宫平滑肌肉瘤子宫内膜免疫染色足叶乙甙妇科恶性肿瘤视觉症状影像学周

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白介素1A基因多态性增加了阿尔茨海默病患者颅内小神经胶质细胞活性

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世界核心医学期刊文摘（神经病学分册） 2005年第2期1卷 41-42页

作者： Hayes A. Green E.K. Pritchard A. Prof. D.M.A. Mann 郭俊 Neuroscience Research Group University of Manchester Hope Hospital Manchester M6 8HD United Kingdom

Objective: To investigate the impact of possession of the -889 C/T polymorphi sm of the interleukin 1A gene (IL-1A) and the -511 C/T polymorphism of the int erleukin 1B gene (IL-1B) on the extent of neuroinflammation in the brain in Alz heimers disease (AD), as demonstrated by the degree of microglial cell activit y associated with each IL-1A and IL-1B genotype. Method: Microglial cell activ ity within the frontal cortex was determined in 68 patients with necropsy confir med AD by image analysis as the percentage area of tissue occupied by ferritin i mmunostained material (microglial cell load). IL-1A, IL-1B, and apolipoprotein E (APOE) genotyping were performed by polymerase chain reaction on DNA extracte d from frontal cortex or cerebellum. Results: The microglial cell load was 31%g reater in patients with IL-1A T allele, 62%greater with IL1A TT genotype, but 108%greater with IL-1A TT genotype in combination with APOE 4 allele. No effec ts on microglial cell load occurred with polymorphisms in IL-1B, or APOE alone. Conclusions: Polymorphisms within IL-1A influence the degree of brain microgli al cell activation, especially in bearers of APOE 4 allele, reinforcing the impo rtance of neuroinflammatory processes in the pathogenesis of AD, and supporting the rationale for treating the disease with inflammation modulating drugs.

关键词：阿尔茨海默病神经胶质细胞基因多态性内小额叶皮质载脂蛋白颅内神经免疫染色炎症程度聚合酶链反应

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