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Disposable Electrochemical Biosensor for Amperometric Measurement of β-hydroxybutyrate and Total Cholesterol

稀有金属材料与工程 2006年第A3期35卷 305-308页

作者： Huaqing Li Li Wang Nenggao Rao Dafu Cui Xinxia Cai State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100080 China Graduate School of Chinese Academy of Science Beijing 100080 China

A new type of disposable thin-film amperometric biosensor has been developed for measurement ofβ-hydroxybutyrate and total cholesterol in *** biosensor consists of two plain gold electrodes mounted on a Polyethylene terephthalate (PET) *** reagent solution contains bienzymes (cholesterol oxidase and cholesterol esterase for total cholesterol,β-hydroxybutyrate dehydrogenase and diaphorase forβ-hydroxybutyrate respectively) with mediator (ferricyanide) were absorption at the surface of electrodes coated by electrodepositing platinum *** presence of the mediator lowers the applied potential and eliminates the interference from other oxidizable species enhancing the sensitivity and selectivity of the biosensor without modifying the dynamic parameters of the *** enzymes stably retains in the matrices of platinum black film improving the performance of the mediated *** linearity is observed in the concentration range from 1.0×10^(-4) to 1.0×10^(-2) mM and 1.0×10^(-4) mM to 4.99×10^(-3) mM with sensitivity of 1.958μA/mM and 2.447μA/mM *** optimized biosensor exhibits excellent reproducibility and stability retaining more than 90% of its original activity over a period of one *** simple operation of the biosensor mass-produced at low cost is expected to find clinical application and homecare.

关键词： amperometric β-hydroxybutyrate total cholesterol ferricyanide platinum black

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Disposable Electrochemical Biosensor for Amperometric Measurement of β-hydroxybutyrate and Total Cholesterol

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稀有金属材料与工程 2006年第S3期 305-308页

作者： Huaqing Li~(1,2) Li Wang~1 Nenggao Rao~1 Dafu Cui~1 Xinxia Cai~(1,2) (1.State Key Laboratory of Transducer Technology,Institute of Electronics,Chinese Academy of Sciences,Beijing 100080,China) (2.Graduate School of Chinese Academy of Science,Beijing 100080,China)

A new type of disposable thin-film amperometric biosensor has been developed for measurement ofβ-hydroxybutyrate and total cholesterol in *** biosensor consists of two plain gold electrodes mounted on a Polyethylene terephthalate （PET） *** reagent solution contains bienzymes （cholesterol oxidase and cholesterol esterase for total cholesterol,β-hydroxybutyrate dehydrogenase and diaphorase forβ-hydroxybutyrate respectively） with mediator （ferricyanide） were absorption at the surface of electrodes coated by electrodepositing platinum *** presence of the mediator lowers the applied potential and eliminates the interference from other oxidizable species enhancing the sensitivity and selectivity of the biosensor without modifying the dynamic parameters of the *** enzymes stably retains in the matrices of platinum black film improving the performance of the mediated *** linearity is observed in the concentration range from 1.0×10-4to 1.0×10-2mM and 1.0×10-4mM to 4.99×10-3mM with sensitivity of 1.958μA/mM and 2.447μA/mM *** optimized biosensor exhibits excellent reproducibility and stability retaining more than 90% of its original activity over a period of one *** simple operation of the biosensor mass-produced at low cost is expected to find clinical application and homecare.

关键词： amperometric β-hydroxybutyrate total cholesterol ferricyanide platinum black

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β-hydroxybutyrate,glucose,calcium,phosphorus,and vitamin C concentrations in blood of dairy cows with subclinical ketosis during the early lactation

β-hydroxybutyrate,glucose,calcium,phosphorus,and vitamin C ...

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中国畜牧兽医学会家畜内科学分会2009年学术研讨会

作者： Zhigang Zhang~(1,2),Guowen Liu~1,Xiaobing Li~1,Zhe Wang~(1*) 1 College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062 2 College of Veterinary Medicine,Northeast Agriculture University,Harbin,150030

The objective of this study was to examine the association betweenβ-hydroxybutyrate(BHBA) and glucose,Calcium(Ca),phosphorus(P),and vitamin C concentrations in dairy healthy and with subclinical ketosis cows during an early lactation *** blood from 99 healthy cows and 26 subclinically ketotic cows within the first two months of lactation was *** concentration of BHBA was measured by enzymatic-rate method,glucose by oxidase method,and P by colorimetric method. These tests were performed in a biochemical *** Ca concentration was measured by atomic absorption spectrometer followed by acid *** C was measured by *** results showed that serum BHBA was significantly increased(P0.05).The serum BHBA concentration was negatively correlated with the serum glucose and Ca concentrations(P<0.01).Vitamin C and P status of cows with subclinical ketosis was not affected by *** Ca concentration can be used as a reference index for the diagnosis of subclinical ketosis.

关键词： dairy cows subclinical ketosis β-hydroxybutyrate calcium glucose vitamin C.

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Construction of a sustainable 3-hydroxybutyrate-producing probiotic Escherichia coli for treatment of colitis

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Cellular & Molecular Immunology 2021年第10期18卷 2344-2357页

作者： Xu Yan Xin-Yi Liu Dian Zhang Yu-Dian Zhang Zi-Hua Li Xu Liu Fuqing Wu Guo-Qiang Chen Center for Synthetic and Systems Biology Tsinghua UniversityBeijingChina School of Life Sciences Tsinghua UniversityBeijingChina MOE Key Lab for Industrial Biocatalysis Dept.Chemical EngineeringTsinghua UniversityBeijingChina

Colitis is a common disease of the colon that is very difficult to *** bacteria could be an effective *** probiotic Escherichia coli Nissle 1917(EcN)was engineered to synthesize the ketone body(R)-3-hydroxybutyrate(3HB)for sustainable production in the gut lumen of mice suffering from *** of heterologous 3HB synthesis routes were constructed,expressed,optimized,and inserted into the EcN genome,combined with deletions in competitive branch *** genome-engineered EcN produced the highest 3HB level of 0.6 g/L under microaerobic *** live therapeutic was found to colonize the mouse gastrointestinal tract over 14 days,elevating gut 3HB and short-chain-length fatty acid(SCFA)levels 8.7-and 3.1-fold compared to those of wild-type EcN,*** sustainable presence of 3HB in mouse guts promoted the growth of probiotic bacteria,especially Akkermansia spp.,to over 31%from the initial 2%of all the *** a result,the engineered EcN termed EcNL4 ameliorated colitis induced via dextran sulfate sodium(DSS)in *** to wild-type EcN or oral administration of 3HB,oral EcNL4 uptake demonstrated better effects on mouse weights,colon lengths,occult blood levels,gut tissue myeloperoxidase activity and proinflammatory cytokine ***,a promising live bacterium was developed to improve colonic microenvironments and further treat *** proof-of-concept design can be employed to treat other diseases of the colon.

关键词： Escherichia coli Nissle 1917 Colitis β-hydroxybutyrate 3HB Synthetic biology Metabolic engineering Microbiomes Probiotics Colonic microenvironment

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miR-222 targets ACOX1, promotes triglyceride accumulation in hepatocytes

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Hepatobiliary & Pancreatic Diseases International 2019年第4期18卷 360-365页

作者： Jing-Jie Wang Yi-Tong Zhang Yu Jen Tseng Jun Zhang Department of Digestive Diseases Huashan Hospital Fudan University

Background:Non-alcoholic fatty liver disease(NAFLD)is one of the most prevalent chronic liver ***,the exact pathogenesis of NAFLD remains to be *** the association with tumors and cardiovascular diseases,the role of miR-222 in NAFLD remains *** present study was to investigate the role of miR-222 in ***:Wild-type C57BL/6 mice were fed a high-fat diet for 12 weeks to induce *** human liver cell line(L02)was cultured with free fatty acid(FFA)-containing medium to stimulate cell *** mRNA levels of miR-222 and acyl Coenzyme A xidase 1(ACOX1)were detected by quantitative-PCR(Q-PCR).The prediction of ACOX1 as the target gene for miR-222 was conducted via *** overexpression or inhibition of miR-222 was mediated by miR-222 mimics or antagomir,and intracellular triglyceride levels were measured using a triglyceride *** reporter assays verified ACOX1 as the target gene for ***:miR-222 was significantly elevated in both the in vivo and in vitro NAFLD *** of miR-222 significantly increased triglyceride content in the L02 cells,while inhibition of miR-222 expression restricted the accumulation of *** of miR-222 significantly inhibited ACOX1 *** transfection assays verified that ACOX1 3-UTR luciferase reporter activity could be inhibited by miR-222 ***:The present study suggested that miR-222 promotes the accumulation of triglycerides by inhibiting ACOX1.

关键词： ACOX1 Hepatocyte steatosis miR-222 NAFLD β-hydroxybutyrate

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Emerging roles of GPR109A in regulation of neuroinflammation in neurological diseases and pain

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Neural Regeneration Research 2023年第4期18卷 763-768页

作者： Kyle Taing Lawrence Chen Han-Rong Weng Department of Basic Sciences California Northstate University College of MedicineElk GroveCAUSA

Neuroinflammation plays a critical role in the pathological process of multiple neurological disorders and pathological pain ***109A,a Gi protein-coupled receptor,has emerged as an important therapeutic target for controlling inflammation in various tissues and *** this review,we summarized current data about the role of GPR109A in ***,we focused on the pharmacological features of GPR109A and signaling pathways used by GPR109A to ameliorate neuroinflammation and symptoms in Alzheimer’s disease,Parkinson’s disease,multiple sclerosis,stroke,and pathological pain conditions.

关键词： β-hydroxybutyrate cytokines HCAR2 lupus neuroinflammation neuropathic niacin nociception synaptic

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Effects of ketogenic diet and ketone bodies on the cardiovascular system:Concentration matters

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World Journal of Diabetes 2020年第12期11卷 584-595页

作者： Souad Nasser Varvara Vialichka Marta Biesiekierska Aneta Balcerczyk Luciano Pirola Carmen Laboratory INSERM Unit 1060—Lyon 1 UniversityPierre Benite 69310France Faculty of Biology and Environmental Protection Department of Molecular BiophysicsUniversity of LodzLodz 90-236Poland The University of Lodz Doctoral School of Exact and Natural Sciences Lodz 90-237Poland

Ketone bodies have emerged as central mediators of metabolic health,and multiple beneficial effects of a ketogenic diet,impacting metabolism,neuronal pathologies and,to a certain extent,tumorigenesis,have been reported both in animal models and clinical *** bodies,endogenously produced by the liver,act pleiotropically as metabolic intermediates,signaling molecules,and epigenetic *** endothelium and the vascular system are central regulators of the organism’s metabolic state and become dysfunctional in cardiovascular disease,atherosclerosis,and diabetic micro-and macrovascular *** physiological circulating ketone bodies can attain millimolar concentrations,the endothelium is the first-line cell lineage exposed to *** in diabetic ketoacidosis high ketone body concentrations are detrimental to the vasculature,recent research revealed that ketone bodies in the low millimolar range may exert beneficial effects on endothelial cell(EC)functioning by modulating the EC inflammatory status,senescence,and ***,we review the long-held evidence of detrimental cardiovascular effects of ketoacidosis as well as the more recent evidence for a positive impact of ketone bodies—at lower concentrations—on the ECs metabolism and vascular physiology and the subjacent cellular and molecular *** also explore arising controversies in the field and discuss the importance of ketone body concentrations in relation to their *** low concentration,endogenously produced ketone bodies upon uptake of a ketogenic diet or supplemented ketone bodies(or their precursors)may prove beneficial to ameliorate endothelial function and,consequently,pathologies in which endothelial damage occurs.

关键词： Endothelial cells Ketone bodies Vascular physiology β-hydroxybutyrate β-Hydroxybutyrylation Histone post-translational modifications

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