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Development of the model of pancreatic β-cell membrane chromatography and its chromatographic characteristics

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Chinese Science Bulletin 2005年第21期50卷 2427-2431页

作者： YANG Guangde HE Langchong BIAN Xiaoli ZHAO Liang Department of Pharmacy School of Medicine Xi'an Jiaotong University Xi'an 710061 China

A new model of pancreatic β-cell membrane chromatography has been established by using a β-cell membrane stationary phase (β-CMSP) prepared by immobi-lizing the β-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the β-CMSP were detected respectively. The surface characteris-tics of the β-CMSP were tested by using the scanning elec-tron microscope and surface energy spectrometer. In this model, the column (10 mm × 2 mm, I.D.) packed with β-CMSP, 25 mmo1/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 ± 0.5℃ were used in the following studies. The retention char-acteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chro-matographic conditions above. The affinities of the sulfony-lureas on β-cell membrane and receptor will be expressed by using the logk′ values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also.

关键词：胰腺细胞细胞隔膜色析法低血糖症蛋白质水平

Functional Polymers for Chromatographic Enantiomer Separation

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复旦学报（自然科学版） 2005年第5期44卷 653-654页

作者： S.Allenmark L.Thunberg Department of Chemistry Gteborg UniversitySE-41296 GteborgSwedenMedicinal ChemistryAstraZeneca R&D MlndalSE-43183 MlndalSweden

Today, methods to obtain pure enantiomers of organic compounds are of extreme importance, not the least due to the demands imposed on the pharmaceutical industry. Separation of the two enantiomers in a racemate is necessary in order to study possible differences in biological activity. Consequently, research on methods for direct chromatographic resolution by means of columns containing a chiral stationary phase (CSP) has been intense during the last decades and a wide variety of CSPs,many of which commercially available,exist *** CSPs have been made from naturally occurring polymers (cellulose, amylose) via suitable derivatization, as well as either by polymerization of chiral monomers or via asymmetric catalysis to yield linear, isotactic polymers of a predominantly single-handed helicity. An excellent review of the field[1], with special emphasis on preparative applications, was published in 1994.

关键词：聚合体色析法对映体分离固定相有机物合成方法

Biochemical, chromatographic and spectroscopic analyses of Suwannee River fulvic acid—Evidence for the presence of a DNA-like material

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Chinese Journal Of Geochemistry 2006年第B8期25卷 267-267页

作者： Nawab ALI Graduate Institute of Technology University of Arkansas at Little Rock 2801 S. Univ. Ave. Little Rock AR 72204 USA