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Isolation of Chloroplasts from Marine Microalga Isochrysis galbana Parke for Their Lipid Composition Analysis

Journal of Ocean University of China 2022年第1期21卷 225-235页

作者： ZHONG Ye LI Yanrong XU Jilin CAO Jiayi ZHOU Chengxu YAN Xiaojun Key Laboratory of Applied Marine Biotechnology Ningbo UniversityNingbo 315211China Collaborative Innovation Center for zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China Fujian Baozhi Aquatic science and Technology Co. Ltd.Zhangzhou 363503China Ningbo Institute of Oceanography Ningbo 315211China

Marine microalga Isochrysis galbana is an important feed species with a high nutritional *** from other uni-cellular algae,its cell contains two chloroplasts which are the major sites for lipid ***,we optimized a chloroplast isola-tion approach suitable for the isolation of *** chloroplasts and determined the purity and integrity of the isolated chloroplasts through microscopic observations and enzyme activity *** chloroplast lipids were analyzed with a ultrahigh-performance li-quid chromatography-Q Exactive Orbitrap-mass *** newly developed isolation approach is simple and reliable to isolate chloroplasts with high integrity and *** average yield of intact chloroplasts was 15.3%±0.1%.Glycolipids and acyl-glycerols were the main chloroplast *** accounted for 56.6%of chloroplast ***(DGDG),monogalactosyldiacylglycerol(MGDG)and sulfoquinovosyldiacylglycerol(SQDG)were the main *** fatty acyl R1/R2 were mostly 18:4/16:1,18:3/16:1 and 18:4/18:5 in DGDGs,14:0/18:4,18:4/18:5,18:4/18:4 and 18:3/18:4 in MGDGs and 16:0/14:0,16:0/18:3,and 18:4/18:3 in *** addition,diacylglycerol(DAG)was the most abundant acylglycerols;the content of 22:6/18:4-DAG was the *** was a little amount of glycosphingolipid(GSL)in ***(DGMG),monogalactosylmonoglyceride(MGMG),sulfoquinovosylmonoacylglycerol(SQMG),monoglyceride(MAG),phospholi-pids(PLs),ceramide(Cer)and betaine lipids were nearly undetectable in *** fatty acid proportions of DGDGs,MGDGs,SQDGs,DAGs,triglycerides(TAGs)and GSLs were either higher or lower than or similar to those of ***,our isolation approach is applicable to many aspects of chloroplast biology,and may offer a reference for the isolation of chloroplasts from other marine microalgae.

关键词： Isochrysis galbana chloroplast isolation method lipid analysis chloroplast lipid

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