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Trans-cinnamaldehyde inhibits Penicillium italicum by damaging mitochondria and inducing apoptosis mechanisms

Food Science and Human Wellness 2022年第4期11卷 975-981页

作者： Fangwei Yang Jiaqi Mi Fei Huang Prompong Pienpinijtham Yahui Guo Yuliang Cheng Weirong Yao Yunfei Xie School of Food Science and Technology Jiangnan UniversityWuxi 214122China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China Qingdao Special Food research Institute Qingdao 266109ShandongChina

Plant-derived essential oils have excellent antifungal effects and can be used for the preservation of fresh foods such as fruits and vegetables, but the detailed mechanism has not been fully elucidated. In this study, we investigated the inhibitory effects of trans-cinnamaldehyde on Penicillium italicum, a common pollution fungus in citrus, and explored the antifungal mechanism of trans-cinnamaldehyde by detecting fungal oxidative damage, mitochondrial metabolism, and cell apoptosis. These results showed that transcinnamaldehyde made the carboxylic acid cycle deregulated by altering the related enzyme activities(succinate dehydrogenase, malate dehydrogenase) and mid product. Moreover, the level of reactive oxygen species rose sharply while the redox level was out of regulation. The mitochondrial membrane potential collapsed, leading to the leakage of cytochrome c, and then triggering the activation of apoptotic protease, which was further confirmed by the significant increase in caspase-3 activity from(3.6 ± 0.6) U to(8.8 ± 1.1) U(P < 0.05). The cytochrome c in mitochondria was detected by confocal Raman microspectroscopy, the characteristic intensity index(I750/I2944) was decreased, indicating that the cytochrome c in mitochondria was reduced and leakage. Besides, the strong negative correlation between Raman intensity and the amount of cytochrome c leakage was established with the correlation coefficient of-0.981 7. This study revealed that destroying the integrity of the mitochondrial membrane, activating the mitochondrial-mediated apoptosis pathway was the in-depth antifungal mechanism of trans-cinnamaldehyde;and Raman spectroscopy technology provided new ideas to study this process with high sensitivity determination of cytochrome c.

关键词： trans-cinnamaldehyde Penicillium italicum Raman microspectroscopy Inhibition mechanism Cytochrome c Apoptosis

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Establishment of the thin-layer chromatography-surface-enhanced Raman spectroscopy and chemometrics method for simultaneous identification of eleven illegal drugs in anti-rheumatic health food

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Food Bioscience 2022年第5期49卷 556-568页

作者： Fangwei Yang Cheng Wang Hang Yu Yahui Guo Yuliang Cheng Weirong Yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Joint International research Laboratory of Food Safety Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

The risk of the illegal addition of anti-inflammatory and analgesic chemical drugs in anti-rheumatic health foods should not be *** supervision and rapid on-site detection technology need to be ***-layer chromatography-surface-enhanced Raman spectroscopy(TLC-SERS),which has the advantages of simple operation,fast separation,and qualitative and quantitative detection,was used in this *** these eleven chemical drugs(acetaminophen,acetylsalicylic acid,aminophenazone,dexamethasone,diclofenac sodium,hydrocortisone,indometacin,naproxen,phenylbutazone,piroxicam,prednisone 21-acetate)that may be added to anti-rheumatic health foods have been simultaneously identified by TLC-SERS combined with chemometrics *** characteristic signals of the separated drug spots were collected by SERS,which were optimized by the gold colloidal nanoparticles’volume and integration *** SERS was subjected to principal component analysis(PCA)to reduce dimensionality and combined with the pattern recognition methods in chemometrics,such as PCA-Linear Discriminant Analysis(LDA),PCA-K Nearest Neighbor and PCA-Support Vector Machine,and eleven drug components were judged and ***,the predictive performances of different models were also analyzed and *** results showed that the TLC plate and four organic solvents of petroleum ether,chloroform,ethyl acetate and acetic acid were selected as the developing *** dropping amount of gold colloidal nanoparticles and the integration time were set and *** limit of detection of the simultaneous detection method of SERS was 10-100 mg/***,SERS was preprocessed by Gap-Segment 2nd Derivative,and then the PCA-LDA model was established,and the model’s prediction accuracy can reach 100%.The method is simple,rapid,sensitive and accurate,and has low experimental instruments and equipment *** is suitable for the on-site simultaneous detection of various anti-inflammato

关键词： TLC-SERS Health food Illegal drugs Chemometrics Simultaneous identification Rapid detection

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Protein corona formed on the TiO_(2)nanoparticles promotes the hydrolysis of collagen in simulated gastrointestinal fluids

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Food Bioscience 2023年第3期53卷 2616-2625页

作者： Yulun Chen Qingrun Liu Hang Yu Yahui Guo Yuliang Cheng He Qian Yunfei Xie Weirong Yao State Key Laboratory of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Engineering research Center of Dairy Quality and Safety Control Technology Ministry of EducationInner Mongolia UniversityNo.235 Daxue West RoadHohhot010021Inner Mongolia Autonomous RegionChina School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Joint International research Laboratory of Food Safety Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

Titanium dioxide nanoparticles(TiO_(2) NPs)could interact with proteins in foods and gastrointestinal fluids,thus changing the properties and functions of *** research articles have only studied the interactions between TiO_(2) NPs and digestive enzymes or the gastrointestinal fate of TiO_(2) NPs without considering the effect of food ***,the interactions between TiO_(2) NPs and trypsin and its effects on the digestion of collagen in vitro were *** showed that the functionalization of amino acids(Asp,and Ser)significantly harmed the adsorption of trypsin by TiO_(2) *** fluorescence quenching and isothermal titration calorimetry confirmed that the hydrogen bond and van der Waals force played an essential role in the interactions between TiO_(2) NPs and ***β-sheet content of trypsin bound to TiO_(2) NPs decreased from 52.40%to 50.73%while the random coil content increased from 20.20%to 22.63%.Binding sites were identified by molecular ***,the digestibility of collagen was improved in vitro in the presence of TiO_(2) NPs analyzed by SDS-PAGE,hydroxyproline quantification,and the antioxidation of digested *** concentra-tion increased by nearly 25%and ABTS radical scavenging rate increased by 50%.This study provides a new insight into the impact of nanoparticles on gastrointestinal digestion.

关键词： TiO_(2)nanoparticles Protein corona Gastrointestinal digestion

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A novel malachite green staining approach for evaluating the internal freshness of egg by detecting the quality of egg cuticle

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Food Bioscience 2022年第6期50卷 814-822页

作者： Zixuan Xu Yuliang Liu Fangwei Yang Lijun Huang Hang Yu Weirong Yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxiJiangsu214122China School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Wuxi Food Safety Inspection and Test Center 35-210 Changjiang South RoadWuxi214142Jiangsu ProvinceChina

The cuticle is the outermost protective film of eggs, whose quality will affect internal freshness. At present, the undamaged detection of eggs freshness relies on hyperspectral imaging technology, which is relatively complicated. In this study, a novel simple approach for evaluating internal freshness of eggs was further explored by detecting the quality of egg cuticle. Malachite green selected as the alternative dye for quality evaluation of adhesive film, the correlation analysis was carried out by collecting the physical and chemical indexes of eggs with different degrees of freshness. It is found that there was a strong positive correlation between the quality of all parts of the cuticle and internal freshness (p < 0.01), and the Pearson correlation coefficient was over 0.85. The Haugh unit and pH of albumen were most strongly correlated with cuticle quality at the top, with the correlation coefficient of 0.919 and −0.906, respectively. The cuticle quality of eggs with contents got worse than that of eggs without contents, and the holes increased when they were stored for 28 days. In this way, malachite green staining method can be used to identify the freshness of egg cuticle, and the quality of egg cuticle can be used to distinguish eggs with different freshness. This study provided a new direction for the study of egg freshness and assisted to distinguish the quality and safety of eggs nondestructively.

关键词： Cuticle Egg freshness Bidirectional effect Quality evaluation Correlation analysis

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