Trans-cinnamaldehyde inhibits Penicillium italicum by damaging mitochondria and inducing apoptosis mechanisms

作     者：Fangwei Yang Jiaqi Mi Fei Huang Prompong Pienpinijtham Yahui Guo Yuliang Cheng Weirong Yao Yunfei Xie Fangwei Yang;Jiaqi Mi;Fei Huang;Prompong Pienpinijtham;Yahui Guo;Yuliang Cheng;Weirong Yao;Yunfei Xie

作者机构：School of Food Science and TechnologyJiangnan UniversityWuxi 214122China State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi 214122China Qingdao Special Food Research InstituteQingdao 266109ShandongChina 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第4期

页      面：975-981页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：supported by China Postdoctoral Science Foundation (2020M680064) National Natural Science Foundation of China (32172326) the Postdoctoral Research Startup Fee of Jiangnan University (1025219032200190) 

主　　题：trans-cinnamaldehyde Penicillium italicum Raman microspectroscopy Inhibition mechanism Cytochrome c Apoptosis 

摘      要：Plant-derived essential oils have excellent antifungal effects and can be used for the preservation of fresh foods such as fruits and vegetables, but the detailed mechanism has not been fully elucidated. In this study, we investigated the inhibitory effects of trans-cinnamaldehyde on Penicillium italicum, a common pollution fungus in citrus, and explored the antifungal mechanism of trans-cinnamaldehyde by detecting fungal oxidative damage, mitochondrial metabolism, and cell apoptosis. These results showed that transcinnamaldehyde made the carboxylic acid cycle deregulated by altering the related enzyme activities(succinate dehydrogenase, malate dehydrogenase) and mid product. Moreover, the level of reactive oxygen species rose sharply while the redox level was out of regulation. The mitochondrial membrane potential collapsed, leading to the leakage of cytochrome c, and then triggering the activation of apoptotic protease, which was further confirmed by the significant increase in caspase-3 activity from(3.6 ± 0.6) U to(8.8 ± 1.1) U(P 0.05). The cytochrome c in mitochondria was detected by confocal Raman microspectroscopy, the characteristic intensity index(I750/I2944) was decreased, indicating that the cytochrome c in mitochondria was reduced and leakage. Besides, the strong negative correlation between Raman intensity and the amount of cytochrome c leakage was established with the correlation coefficient of-0.981 7. This study revealed that destroying the integrity of the mitochondrial membrane, activating the mitochondrial-mediated apoptosis pathway was the in-depth antifungal mechanism of trans-cinnamaldehyde;and Raman spectroscopy technology provided new ideas to study this process with high sensitivity determination of cytochrome c.