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Effect of mechanical stresses on degradation behavior of high-purity magnesium in bone environments

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Journal of Materials Science & Technology 2024年第4期171卷 252-261页

作者： Yan Yao Jie Xia Lizhen Wang Yuanming Gao Xili Ding Chao Wang Yubo Fan Key Laboratory of Biomechanics and Mechanobiology(Beihang University) Ministry of EducationBeijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringSchool of Engineering MedicineBeihang UniversityBeijing 100083China

High-purity(HP)magnesium(Mg)has emerged as a promising biomaterial for supporting functional bone *** previous study found that mechanical stresses and the surrounding fibrotic tissue(subcuta-neous)both play crucial roles in the degradation of HP ***,due to challenges in the degradation and regeneration process in vivo,it remains unclear how stress affects HP Mg degradation in bone en-vironments,limiting its further *** this study,novel loading devices were designed and the effects of tensile and compressive stresses on HP Mg degradation in vivo and in vitro bone environments were quantitatively *** addition,bone osteointegration around HP Mg was explored *** stress increases the degradation rate of HP Mg in vivo and in *** Mg degradation in vivo is more sensitive to stress factors than in vitro,but the sensitivity decreases with corrosion *** volume loss rate of HP Mg is multilinear with the applied stress and degradation *** volume of bone tissue surrounding HP Mg is larger in the no-stress group compared to the stressed groups,which is more pronounced with increasing implantation *** results provide valuable insights for optimiz-ing the design of HP Mg-based implants considering load *** will help to achieve a balance between the degradation rate of the implant and the regeneration rate of the surrounding bone.

关键词： High-purity magnesium Quantitative stresses Degradation Bone environments In vivo and in vitro

Mutation in a non-force-bearing region of protein L influences force-dependent unfolding behavior

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Chinese Physics B 2024年第7期33卷 624-629页

作者：蒋环杰王艳伟陈家媛胡丹潘海郭子龙陈虎 Department of Physics Wenzhou UniversityWenzhou 325035China Center of Biomedical Physics Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325000China Research Institute for Biomimetics and Soft Matter Fujian Provincial Key Laboratory for Soft Functional Materials ResearchDepartment of PhysicsXiamen UniversityXiamen 361005China

Single-molecule magnetic tweezers(MTs) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MTs to measure the force-dependent folding and unfolding rates of both protein L(PLWT) and its Y47W mutant(PLY47W) where the mutation point is not at the force-bearing β-strands. The measurements were conducted within a force range of 3–120 pN. Notably, the unfolding rates of both PLWT and PWY47W exhibit distinct force sensitivities below 50 pN and above 60 pN, implying a two-barrier free energy landscape. Both PLWT and PLY47W share the same force-dependent folding rate and the same transition barriers,but the unfolding rate of PLY47W is faster than that of PLWT. Our finding demonstrates that the residue outside of the force-bearing region will also affect the force-induced unfolding dynamics.

关键词： protein folding magnetic tweezers protein L

Genome-wide identifcation of the histone acetyltransferase gene family in Citrus clementina and its potential roles in citrate metabolism

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Food Quality and Safety 2024年第1期8卷 201-211页

作者： Xiahui Lin Yanna Shi Shengchao Liu Xincheng Liu Minglei Zhang Bo Zhang Shaojia Li Kunsong Chen College of Agriculture and Biotechnology Zhejiang UniversityZijingang CampusHangzhouChina Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology Zhejiang UniversityZijingang CampusHangzhouChina The State Agriculture Ministry Laboratory of Horticultural Plant Growth Development and Quality ImprovementZhejiang UniversityZijingang CampusHangzhouChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Institute of Horticulture Jiangxi Academy of Agricultural SciencesNanchangChina

Histone acetyltransferase(HAT)catalyzes histone acetylation and is involved in plant growth and development and stress ***,37 CitHAT genes were identifed in Citrus *** physicochemical properties,chromosomal location,gene structure,conserved domain and motif,and cis-acting elements were *** were classifed into four subfamilies based on protein sequence homology,which was strongly supported by gene structure,conserved domain,and motif *** cis-acting elements in gene promoter regions were predicted to be associated with the regulation of plant growth,stress resistance,and response to *** and transcriptomic analyses of citrus callus with mock treatment and HAT inhibitor treatment revealed that increased citric acid content in inhibitor treatment may be attributed to differential expression of CitPEPCK2 and CitGS2,which may be due to the hypo-acetylation of histone *** expression patterns of CitHATs in citrus fruit development stages showed that CitHAG11 and CitHAG28 exhibited a negative correlation with citric acid *** study associates the potential function of histone acetyltransferases in citrate metabolism and extends the molecular mechanism of citrate metabolism in fruits.

关键词： Citrus histone acetyltransferases citric acid citrate metabolism histone acetylation.

Design methodology,synthesis,and control strategy of the high-speed planetary rover

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Frontiers of Mechanical Engineering 2024年第2期19卷 59-74页

作者： Renchao LU Haibo GAO Zhen LIU Runze YUAN Zongquan DENG State Key Laboratory of Robotics and Systems Harbin Institute of TechnologyHarbin 150001China

The planned missions to explore the surfaces of the Moon and Mars require high exploration efficiency,thus imposing new demands on the mobility system of planetary *** this paper,a design method for a high-speed planetary rover(HPR)is proposed,and the representative configurations are modeled and ***,the influence of the planetary surface environment on the design of HPRs is analyzed,and the design factors for HPRs are determined by studying a single-wheel ***,a design methodology for HPRs is *** adaptive suspension mechanisms of a four-wheeled rover are synthesized using the all-wheel-attachment condition and position and orientation characteristics theory,which are expressed in the form of a graph theory for the increase in elastic components and active ***,a dynamic model is built,and a simulation is carried out for the proposed *** validity of the proposed method and rover is verified,thus highlighting their potential application in future planetary exploration.

关键词： design methodology spectrum analysis high-speed planetary rover type synthesis

DNA methylation controlling abscisic acid catabolism responds to light to mediate strawberry fruit ripening

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Journal of Integrative Plant Biology 2024年第8期66卷 1718-1734页

作者： Yunfan Sun Xiaofang Yang Rongrong Wu Shouzheng Lv Yunduan Li Haoran Jia Yuying Yang Baijun Li Wenbo Chen Andrew C.Allan Guihua Jiang Yan-Na Shi Kunsong Chen College of Agriculture&Biotechnology Zhejiang UniversityZijingang CampusHangzhou 310058China Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhouZhejiang 310021China Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology Zhejiang UniversityZijingang CampusHangzhou 310058China The State Agriculture Ministry Laboratory of Horticultural Plant Growth Development and Quality ImprovementZhejiang UniversityZijingang CampusHangzhou 310058China New Zealand Institute for Plant&Food Research Limited Private Bag 92169AucklandNew Zealand School of Biological Sciences University of AucklandPrivate Bag 92019AucklandNew Zealand

Phytohormones,epigenetic regulation and environmental factors regulate fruit ripening but their interplay during strawberry fruit ripening remains to be *** this study,bagged strawberry fruit exhibited delayed ripening compared with fruit grown in normal light,correlating with reduced abscisic acid(ABA) *** of the key ABA catabolism gene,ABA 8′-hydroxylase FaCYP707A4,was induced in bagged *** light exclusion whole genome DNA methylation levels were up-regulated,corresponding to a delayed ripening process,while DNA methylation levels in the promoter of FaCYP707A4 were suppressed,correlating with increases in transcript and decreased ABA *** indicated FaCRY1,a blue light receptor repressed in bagged fruit and FaAGO4,a key protein involved in RNA-directed DNA methylation,could bind to the promoter of *** interaction between FaCRY1 and FaAGO4,and an increased enrichment of FaAGO4 directed to the FaCYP707A4 promoter in fruit grown under light suggests FaCRY1 may influence FaAGO4 to modulate the DNA methylation status of the FaCYP707A4 ***,transient overexpression of FaCRY1,or an increase in FaCRY1 transcription by blue light treatment,increases the methylation level of the FaCYP707A4 promoter,while transient RNA interference of FaCRY1 displayed opposite *** findings reveal a mechanism by which DNA methylation influences ABA catabolism,and participates in light-mediated strawberry ripening.

关键词： ABA catabolism DNA methylation light ripening strawberry

Heptamethine cyanines in bioorthogonal chemistry

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Chinese Chemical Letters 2024年第2期35卷 131-137页

作者： Yuanyuan Liao Yuting Liang Yurou Huang Xiaoyan Zeng Tian He Jun Yin national Key Laboratory of Green Pesticide International Joint Research Center for Intelligent Biosensor Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China

Due to their excellent fluorescence properties and biological function,cyanine dyes have been widely applied in biological *** cyanine(Cy7)dyes,as a type of classic near-infrared(NIR)fluorescent dyes,are considered as one of the effective fluorescent tools in the living organisms due to their good biocompatibility and very low background *** reactions performed in living cells and tissues have developed by leaps and bounds in recent *** NIR fluorescent labeling technique involving cyanine has attracted widespread *** review summarizes their recent application in the field of bioorthogonal imaging,mainly concluding Cy7-type dyes,labeling strategy,bioimaging application,*** expect this work can provide some helps for the studies of NIR bioorthogonal reaction in vivo.

关键词： Fluorescent probes Heptamethine cyanines Bioorthogonal reaction Imaging Cell

Propagation and Dispersion Characteristics of Scholte Wave in VTI Media with the Presence of Irregular Seabed

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Journal of Earth Science 2024年第2期35卷 722-725页

作者： Xiaobo Liu Yun Wang Jianlei Zhang Haifeng Chen Hua Zhang School of Ocean Sciences China University of GeosciencesBeijing 100083China Key Laboratory of Polar Geology and Marine Mineral Resources China University of GeosciencesBeijing 100083China School of Geophysics and Information Technology China University of GeosciencesBeijing 100083China Research&Development Center BGP IncCNPCZhuozhou 072751China national Engineering Research Center for Oil and Gas Exploration Computer Software Beijing 100080China

INTRODUCTION Surface waves propagating along the seafloor are general ly called Scholte waves,and were first discovered and studied in the early 1950s(Kugler et al.,2005;Buchen and Ben-Hador,1996).Scholte waves exhibit a dispersion phenomenon,which implies that their velocity varies with ***-frequency Scholte waves can propagate over long distances on the seabed with little attenuation(Bohlen et al.,2004).The particle mo tion of Scholte waves in a solid medium changes from a retro grade to a prograde ellipse(Klein et al.,2005;McMechan and Yedlin,1981).

关键词： seabed Dispersion implies

Taiji-TianQin-LISA network:Precisely measuring the Hubble constant using both bright and dark sirens

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Science China(Physics,Mechanics & Astronomy) 2024年第2期67卷 59-77页

作者： Shang-Jie Jin Ye-Zhu Zhang Ji-Yu Song Jing-Fei Zhang Xin Zhang Key Laboratory of Cosmology and Astrophysics(Liaoning)&College of Sciences Northeastern UniversityShenyang 110819China Key Laboratory of Data Analytics and Optimization for Smart Industry(Ministry of Education) Northeastern UniversityShenyang 110819China national Frontiers Science Center for Industrial Intelligence and Systems Optimization Northeastern UniversityShenyang 110819China

In the coming decades,the space-based gravitational-wave(GW)detectors such as Taiji,TianQin,and LISA are expected to form a network capable of detecting millihertz GWs emitted by the mergers of massive black hole binaries(MBHBs).In this work,we investigate the potential of GW standard sirens from the Taiji-TianQin-LISA network in constraining cosmological *** the optimistic scenario in which electromagnetic(EM)counterparts can be detected,we predict the number of detectable bright sirens based on three different MBHB population models,i.e.,popⅢ,Q3d,and *** results show that the TaijiTianQin-LISA network alone could achieve a constraint precision of 0.9%for the Hubble constant,meeting the standard of precision ***,the Taiji-TianQin-LISA network could effectively break the cosmological parameter degeneracies generated by the CMB data,particularly in the dynamical dark energy *** combined with the CMB data,the joint CMB+Taiji-TianQin-LISA data offerσ(w)=0.036 in the wCDM model,which is close to the latest constraint result obtained from the CMB+SN *** also consider a conservative scenario in which EM counterparts are not *** to the precise sky localizations of MBHBs by the Taiji-TianQin-LISA network,the constraint precision of the Hubble constant is expected to reach 1.2%.In conclusion,the GW standard sirens from the Taiji-TianQin-LISA network will play a critical role in helping solve the Hubble tension and shedding light on the nature of dark energy.

关键词： space-based gravitational wave detection standard sirens the Taiji-TianQin-LISA network the Hubble constant dark energy

Immunological Exploration of Helicobacter pylori Serotype O_(2) O-Antigen by Using a Synthetic Glycan Library

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Chinese Journal of Chemistry 2024年第3期42卷 243-251页

作者： Ming Zhao Guangzong Tian Chunjun Qin Xiaopeng Zou Peter H. Seeberger Jing Hu Jian Yin Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of Biotechnology & School of Life Sciences and Health EngineeringJiangnan UniversityLihu Avenue 1800WuxiJiangsu214122 China Biomolecular Systems Department Max Planck Institute of Colloids and InterfacesAm Mühlenberg 1Potsdam14476 Germany Wuxi School of Medicine Jiangnan UniversityLihu Avenue 1800WuxiJiangsu214122 China

Helicobacter pylori(***)infection is a threat to human *** lipopolysaccharide(LPS)O-antigen holds promise for developing *** is meaningful to explore the immunological activity of oligosaccharides with different lengths and frameshifts for antigen ***,a glycan library of *** O2 O-antigen containing eight fragments is *** screening with *** O2 LPS sera and patients’sera by glycan microarray,the disaccharide HPO2G-2b and trisaccharide HPO2G-3a show strong antigenicity and then are separately conjugated with carrier protein *** glycoconjugates elicit a robust immunoglobulin G(IgG)immune response in *** anti-HPO2G-3a IgG antibodies possess a much stronger binding affinity with the LPS and bacteria of *** O2 than the anti-HPO2G-2b IgG *** is no cross-reaction between both sera IgG antibodies with LPS and bacteria of *** O1,O6,and *** results demonstrate the trisaccharide HPO2G-3a is a promising candidate for *** vaccine development.

关键词： Glycoconjugates Helicobacter pylori Immunology Microarrays O-antigen Oligosaccharides