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Chinese Journal of Chemical Engineering 2021年第11期34卷 211-218页

作者： Yiya Wang Tao Sun Yinzhu Wang Hao Wu Yan Fang Jiangfeng Ma Min Jiang State Key Laboratory of Materials-Oriented Chemical Engineering College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211800China

Exopolysaccharides can be produced by various bacteria and have important biological roles in bacterial survival depend on molecular weight,linkage,and *** this study,Leuconostoc pseudomesenteroides G29 was identified and found to produce two types of exopolysaccharides from sucrose including soluble and insoluble *** regulation of pH above 5.5,soluble a-glucan production was increased to 38.4 g∙L^(-1) from 101.4 g∙L^(-1) sucrose with fewer accumulation of lactic acid and acetic ***,the quantity of thick white precipitate,that is insoluble a-glucan,was also ***,a-glucans were prepared by enzymatic reaction with crude glucansucrases from the supernatant of G29 fermentation broth and purified for structure *** on the integration analysis of FT-IR and NMR,it was observed that soluble a-glucan is a highly linear dextran with α-1,6 glycosidic bonds while the insoluble a-glucan has 93%of α-1,3 and 7%of α-1,6 glycosidic *** results extend our understanding of exopolysaccharides production by ***,and this water insoluble α-1,3-glucan might have potential application as biomaterials and/or biochemicals.

关键词： Exopolysaccharide Glucansucrase Leuconostoc pseudomesenteroides Insolubleα-glucan a-1,3 glycosidic bond

Aureobasidium subglaciale F134 is a bifunctional whole-cell biocatalyst for Baeyer-Villiger oxidation or selective carbonyl reduction controllable by temperature

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Chinese Journal of Chemical Engineering 2020年第12期28卷 3044-3051页

作者： Liqun Shen Ran Cang Guang Yang Anqi Zeng He Huang Zhigang Zhang School of Pharmaceutical Sciences Nanjing Tech UniversityNanjing 211816China State Key Laboratory of Materials-Oriented Chemical Engineering Nanjing Tech UniversityNanjing 211816China

The microbial production of either ester/lactones or enantio-enriched alcohols through Baeyer-Villiger oxidation or stereoselective reduction of ketones,respectively,is possible by using whole cells of *** F134 as a bifunctional *** chemoselective pattern of acetophenone biotransformation catalyzed by these cells can be regulated through reaction temperature,directing the reaction either towards oxidation or reduction *** Baeyer–Villiger oxidation activity of *** F134 whole cells is particularly dependent on reaction *** was transformed efficiently to phenol via the primary Baeyer–Villiger product phenyl acetate at 20℃ after 48 h with 100% *** contrast,at 35℃,enantio-enriched(S)-1-phenylethanol was obtained as the sole product with 64% conversion and 89% *** addition,*** F134 cells also catalyze the selective reduction of various structurally different aldehydes and ketones to alcohols with 40% to 100% yield,indicating broad substrate spectrum and good enantioselectivity in relevant *** study provides a bifunctional biocatalyst systemthat can be used in Baeyer–Villiger oxidation aswell as in asymmetric carbonyl reduction,setting the stage for future work concerning the identification and isolation of the respective enzymes.

关键词： biocatalysis Whole cells Baeyer-Villiger oxidation Aureobasidium subglaciale Carbonyl reduction

New KAT6 inhibitors induce senescence and arrest cancer growth

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Synthetic and Systems biotechnology 2018年第4期3卷 244-245页

作者： Fei Huang School of Pharmaceutical Sciences Nanjing Tech UniversityNanjingChina

Lysine acetyltransferases(KATs)catalyze lysine acetylation,a reversible protein modification implicated in a wide variety of disease *** acetyltransferases(HATs)comprise a KAT sub-class that acetylate specific lysines in histones,hence playing an important role in the regulation of chromatin organization and *** are critical regulators of signaling in many diseases,including ***6A(also known as monocytic leukemia zinc finger protein,MOZ)and KAT6B(also known as MORF and QKF)belong to the MYST family of HATs,that comprise KAT5–*** are the targets of chromosomal translocations identified in acute myeloid leukaemia and various *** seems logical therefore that inhibition of KAT6A and KAT6B may provide a therapeutic benefit in *** et *** a new class of anti-cancer drug that can put cancer cells into a permanent sleep or senescence,using high-throughput screening followed by medicinal chemistry optimization,in-cell assays,biochemical assessment of target engagement,and tumour models in mice and *** research showed promise in arresting tumour growth in pre-clinical models of blood and liver cancers as well as delaying or stopping relapse without damaging the cells'DNA or some harmful side-effects caused by chemotherapy and radiotherapy.

关键词： hence permanent senescence

Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals:current status and future perspectives

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Green Chemical Engineering 2023年第2期4卷 199-211页

作者： Jing Wang Ruirui Qin Yuanke Guo Chen Ma Xin Wang Kequan Chen Pingkai Ouyang State Key Laboratory of Materials-Oriented Chemical Engineering College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing211816China

Methanol is becoming an attractive fermentation feedstock for large-scale bioproduction of chemicals,due to its natural abundance and mature production *** methylotrophs,which can utilize methanol as the only source of carbon and energy,are ideal hosts for methanol bioconversion due to their high methanol utili-zation rate and have been extensively employed in the production of value-added chemicals from ***,we review the natural methanol utilization pathways in native methylotrophs,describing the available synthetic biology tools developed for engineering native methylotrophs,and discuss the strategies for improving their methanol utilization ***,the representative examples of engineering the native methylotrophs to produce value-added products from methanol are ***,we also discuss the major challenges and possible solutions for the application of native methylotrophs in methanol-based biomanufacturing.

关键词： Native methylotrophs Methanol biomanufacturing Value-added chemicals

Microbial production of L-malate from renewable non-food feedstocks

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Chinese Journal of Chemical Engineering 2021年第2期34卷 105-111页

作者： Youming Jiang Xiaohan Ye Tianwen Zheng Weiliang Dong Fengxue Xin Jiangfeng Ma Min Jiang State Key Laboratory of Materials-Oriented Chemical Engineering College of Biotechnology and Pharmaceutical EngineeringNanjing Tech UniversityNanjing 211800China

L-malate is an intermediate of the tricarboxylic acid cycle which is naturally occurred in various microorganisms,and it has been widely applied in polymer,beverage and food,textile,agricultural and pharmaceutical *** by the pursuit of a sustainable economy,microbial production of L-malate has received much attention in last *** this review,we focus on the utilization of wastes and/or byproducts as feedstocks for the microbial production of ***,we present the recent developments on the natural or engineered metabolic pathways that dedicate to the biosynthesis of L-malate,and also provide a comprehensive discussions on developing high-efficient ***,the recent achievements in microbial production of L-malate from various carbon sources were concluded and ***,some abundant non-food feedstocks which have been used for microbial production of other chemicals were reviewed,as they may be potential candidate feedstock for L-malate production in ***,we outlined the major challenges and proposed further improvements for the production of L-malate.

关键词： L-malate Renewable feedstock Microbial fermentation bioeconomy C4 dicarboxylic acid

Immobilization of laccase on organic–inorganic nanocomposites and its application in the removal of phenolic pollutants

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Frontiers of Chemical Science and Engineering 2023年第7期17卷 867-879页

作者： Wei Zhang Runtang Liu Xu Yang Binbin Nian Yi Hu State Key Laboratory of Materials-Oriented Chemical Engineering School of Pharmaceutical SciencesNanjing Tech UniversityNanjing 210009China

Polydopamine-functionalized nanosilica was synthesized using an inexpensive and easily obtainable raw material,mild reaction conditions,and simple ***,a flexible spacer arm was introduced by using dialdehyde starch as a cross-linking agent to bind with laccase.A high loading amount(77.8 mg∙g^(‒1))and activity retention(75.5%)could be achieved under the optimum immobilization *** parameters showed that the immobilized laccase had a lower thermal deactivation rate constant and longer *** enhancement of thermodynamic parameters indicated that the immobilized laccase had better thermal stability than free *** residual activity of immobilized laccase remained at about 50.0%after 30 days,which was 4.0 times that of free *** laccase demonstrated excellent removal of phenolic pollutants(2,4-dichlorophenol,bisphenol A,phenol,and 4-chlorophenol)and perfect reusability with 70% removal efficiency retention for 2,4-dichlorophenol after seven *** results suggested that immobilized laccase possessed great reusability,improved thermal stability,and excellent storage ***–inorganic nanomaterials have a good application prospect for laccase immobilization,and the immobilized laccase of this work may provide a practical application for the removal of phenolic pollutants.

关键词： polydopamine pollutant removal thermodynamic phenolic pollutants immobilized laccase

Photoinduced Merging with Copper-or Nickel-Catalyzed 1,4-Cyanoalkylarylation of 1,3-Enynes to Access Multiple Functionalizatized Allenes in Batch and Continuous Flow

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Chinese Journal of Chemistry 2022年第13期40卷 1537-1545页

作者： Qi Sun Xin-Peng Zhang Xiu Duan Long-Zhou Qin Xin Yuan Meng-Yu Wu Jie Liu Shan-Shan Zhu Jiang-Kai Qiu Kai Gu biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjingJiangsu 211816China State Key Laboratory of Materials-Oriented Chemical Engineering Nanjing Tech UniversityNanjingJiangsu 211800China

A three-component reaction of 1,3-enynes and cyclobutanone oxime esters in the presence of aryl boronic acids or organozinc reagents via the photoredox/copper or photoredox/nickel catalysis has been *** redox-neutral 1,4-cyanoalkylarylation reaction has demonstrated mild condition,high catalytic reactivity and wide functional group compatibility,allowing access to a variety of functionalized tetra-substituted allene derivatives with high chemo-and ***,using photocatalytic continuous flow technique to promote this process would result in increased yields(70%in flow vs.61%in batch),reduced reaction times(7 min in flow vs.6 h in batch),and easy scale-up(upgrade to gram scale),showcasing its potential as a synthetic platform.

关键词： Photochemistry 1,4-Cyanoalkylarylation Radical reaction 1,3-Enynes 1,4-Cyanoalkylarylation Tetra-substituted allenes Photocatalytic continuous flow technique

An ultrasonic-ionic liquid process for the efficient acid catalyzed hydrolysis of feather keratin

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Chinese Journal of Chemical Engineering 2019年第3期27卷 660-667页

作者： Song Ding Yang Sun Hongyue Chen Chao Xu Yi Hu School of Pharmaceutical Sciences Nanjing Tech University School of biotechnology and Pharmaceutical Engineering of Nanjing Tech University School of Pharmaceutical Sciences State Key Laboratory of Material-Oriented Chemical Engineering Nanjing Tech University

A novel efficient method for hydrolyzing feather keratin using an ultrasonic-ionic liquid coupling process has been developed, with reaction conditions optimized using response surface analysis of data obtained from single factor optimization studies. Ultrasonic irradiation(225 W power) of feathers in 8.4 mol·L^(-1) hydrochloric acid containing 1-butyl-3-methylimidazolium chloride as a cosolvent at 80 °C for 1.4 h, followed by heating at 110 °C for 8.3 h, resulted in hydrolysis of their keratin component in an excellent 83.1% yield. Compared with previous methods, this new method employs reduced amounts of hydrochloric acid, shorter reaction time, and affords amino acid hydrolysis products in higher yield.

关键词： Ionic liquid Ultrasonic Keratin Hydrolysis Amino acid

Improving Catalytic Performance of Burkholderiacepacia Lipase by Chemical Modification with Functional Ionic Liquids

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Chemical Research in Chinese Universities 2018年第2期34卷 279-284页

作者： XU Chao YIN Xuhan ZHANG Chuan CHEN Hongyue HUANG He HU Yi State Key Laboratory of Materials-Oriented Chemical Engineering School of Pharmaceutical Sciences Nanjing Tech University Nanjing 210009 P. R. China

Various imidazolium and choline-based functional ionic liquids（Ks） comprising different cations and anions were grafted onto Burkholderiacepacia lipase（BCL） through surface amino acids coupling. The catalytic activity, thermostability, organic solvent tolerance and adaptability to temperature and pH changes of the modified BCL were then evaluated in olive oil hydrolysis reaction. The results showed that different combinations of cations and anions in ILs had important influence on the catalytic performance of the modified lipases. BCL modified with IL [Choline][H2PO4] was the most improved lipase, in which increases by 1.2 folds in relative activity, 2.5 folds in typi- cal proton solvent（10% methanol, volume fraction）, and 1.4 folds in thermostability（after incubation at 70℃ for 2 h） were achieved in relative toits native form. BCL modified with [HOOCEPEG350[M][BF4] had higher optimal tempe- rature and pH, and better thermosability compared with the native and other modified BCLs. The conformational changes of BCLs were also confirmed by fluorescence spectroscopy and circular dichroism spectroscopy.

关键词： Burkholderiacepacia lipase Chemical modification Ionic liquid Catalytic activity

Practical Synthesis of Valbenazine via 1,3-Dipolar Cycloaddition

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Chinese Journal of Chemistry 2024年第8期42卷 841-845页

作者： Yalan Peng Zuming Lin Lili Zhu Shiqing Han Sha-Hua Huang Ran Hong College of biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjingJiangsu211816 China School of Environmental and Chemical Engineering Shanghai Institute of TechnologyShanghai201418 China State Key Laboratory of Chemical biology Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032 China innovation Research Institute of Traditional Chinese Medicine(IRI) Shanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai201203 China

Comprehensive Summary,Valbenazine(Ingrezza),a potent and highly selective inhibitor of vesicular monoamine transporter type 2(VMAT2)through the active metabolite hydrotetrabenazine(HTBZ),has been approved for the treatment of tardive dyskinesia and,very recently,for chorea,which is associated with Huntington's *** numerous synthetic efforts dedicated to the synthesis of HTBZ,the industrial preparation of valbenazine uses dihydroisoquinoline as a starting material and the chiral resolution of racemic HTBZ derived from ketone ***,we present a practical synthesis of HTBZ and valbenazine featuring a highly stereoselective 1,3-dipolar cycloaddition and enzymatic kinetic *** cascade process includes cycloaddition,N—O bond cleavage,and lactamization,which proved to be operationally *** allure of the enzymatic resolution developed in this work offers a rapid access toward affording tetrahydroisoquinoline(THIQ)-fused piperidine in the production of medically significant compounds,such as yohimbine and reserpine.

关键词： Cycloaddition Chemoenzymatic Kinetic resolution Prodrugs VMAT2 inhibitor