Aureobasidium subglaciale F134 is a bifunctional whole-cell biocatalyst for Baeyer-Villiger oxidation or selective carbonyl reduction controllable by temperature

作     者：Liqun Shen Ran Cang Guang Yang Anqi Zeng He Huang Zhigang Zhang Liqun Shen;Ran Cang;Guang Yang;Anqi Zeng;He Huang;Zhigang Zhang

作者机构：School of Pharmaceutical SciencesNanjing Tech UniversityNanjing 211816China State Key Laboratory of Materials-Oriented Chemical EngineeringNanjing Tech UniversityNanjing 211816China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2020年第28卷第12期

页      面：3044-3051页

核心收录：

学科分类：081705[工学-工业催化] 08[工学] 0817[工学-化学工程与技术] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：financially supported by the National Natural Science Foundation of China(No.21646014 and 21776134) the program of Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture(XTE1851) 

主　　题：Biocatalysis Whole cells Baeyer-Villiger oxidation Aureobasidium subglaciale Carbonyl reduction 

摘      要：The microbial production of either ester/lactones or enantio-enriched alcohols through Baeyer-Villiger oxidation or stereoselective reduction of ketones,respectively,is possible by using whole cells of *** F134 as a bifunctional *** chemoselective pattern of acetophenone biotransformation catalyzed by these cells can be regulated through reaction temperature,directing the reaction either towards oxidation or reduction *** Baeyer–Villiger oxidation activity of *** F134 whole cells is particularly dependent on reaction *** was transformed efficiently to phenol via the primary Baeyer–Villiger product phenyl acetate at 20℃ after 48 h with 100% *** contrast,at 35℃,enantio-enriched(S)-1-phenylethanol was obtained as the sole product with 64% conversion and 89% *** addition,*** F134 cells also catalyze the selective reduction of various structurally different aldehydes and ketones to alcohols with 40% to 100% yield,indicating broad substrate spectrum and good enantioselectivity in relevant *** study provides a bifunctional biocatalyst systemthat can be used in Baeyer–Villiger oxidation aswell as in asymmetric carbonyl reduction,setting the stage for future work concerning the identification and isolation of the respective enzymes.