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Nanoliposome-encapsulated caged-GABA for modulating neural electrophysiological activity with simultaneous detection by microelectrode arrays

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Nano Research 2020年第6期13卷 1756-1763页

作者： Jingyu Xie Yilin Song Yuchuan Dai Ziyue Li Fei Gao Xuanyu Li Guihua Xiao Yu Zhang Hao Wang Zeying Lu Xingyu Jiang Wenfu Zheng Xinxia Cai State key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing100190China University of Chinese Academy of sciences Beijing100049China Beijing Engineering Research Center for BioNanotechnology CAS Key Laboratory for Biological Effects of Nanomaterials and NanosafetyCAS Center for Excellence in NanoscienceNational Center for NanoScience and TechnologyBeijing100190China Department of Biomedical Engineering Southern University of Science and TechnologyShenzhen518055China

Effective and precise neural modulation with real-time detection in the brain is of great importance and represents a significant ***-encapsulated light-sensitive compounds have excellent characteristics such as high temporal and spatial resolution,delayed drug clearance,and restricted drug biodistribution for neural *** this study,we developed a nanoliposome-based delivery system for ruthenium-based caged GABA compounds(Nanolipo-Ru)to modulate neural activity and allow for real-time monitoring using the microelectrode arrays(MEAs).The Nanolipo-Ru nanoparticles had an average size of 134.10±4.30 nm and exhibited excellent stability for seven *** the in vivo experiment in the rat,release of GABA by Nanolipo-Ru under blue light illumination resulted in an average firing rate reduction in interneurons and pyramidal neurons in the same brain region of 79.4%and 81.6%,***,the average power of local field potentials in the 0–15 Hz range degraded from 4.34 to 0.85 *** addition,the Nanolipo-Ru nanoparticles have the potential to provide more flexible timing of modulation than unencapsulated RuBi-GABA in the *** results indicated that Nanolipo-Ru could be an effective platform for regulating neuronal ***,nanoliposomes with appropriate modifications would render promising utilities for targeting of specific types of neurons in the future.

关键词： neural modulation nanoliposome microelectrode array light-sensitive drug

Characterization of Scanning SQUID Probes Based on 3D Nano-Bridge Junctions in Magnetic Field

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Chinese Physics Letters 2020年第8期37卷 31-35页

作者： Yin-Ping Pan Yue Wang Ruo-Ting Yang Yan Tang Xiao-Yu Liu Hua Jin Lin-Xian Ma Yi-Shi Lin Zhen Wang Jie Ren Yi-Hua Wang Lei Chen Center for Excellence in Superconducting Electronics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Department of Physics and State key Laboratory of Surface Physics Fudan UniversityShanghai 200438China University of Chinese Academy of sciences Beijing 100049China Shanghai Research Center for Quantum sciences Shanghai 201315China

We develop superconducting quantum interference device(SQUID)probes based on 3D nano-bridge junctions for the scanning SQUID *** use of these nano-bridge junctions enables imaging in the presence of a high magnetic ***,a superconducting ground layer has been employed for better magnetic *** our study,we prepare a number of scanning SQUID probes with and without a ground layer to evaluate their performance in external magnetic *** devices show the improved magnetic modulation up to 1.4 *** is found that the ground layer reduces the inductance,and increases the modulation depth and symmetricity of the gradiometer design in the absence of the ***,the layer is not compatible with the use of the scanning SQUID probe in the field because it decreases its working field ***,by adding the layer,the mutual inductance between the feedback coil and the SQUID also decreases linearly as a function of the field.

关键词： SQUID layer Probe

Copper-Mediated Di- and Monofluoromethanesulfonylation of Arenediazonium Tetrafluoroborates： Probing the Fluorine Effect

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Chinese Journal of Chemistry 2018年第3期36卷 206-212页

作者： Bo Xing Chuanfa Ni Jinbo Hu key Laboratory of Organofluorine Chemistry Center for Excellence in Molecular Synthesis Shanghai Institute of Organic Chemistry University of Chinese Academy of Sciences Chinese Academy of Sciences 345Lingling Lu Shanghai 200032 China

A copper-mediated di- and monoffuoromethanesulfonylation of arenediazonium tetrafluoroborates using di- and monofluoromethanesul- finate reagents provides aryl difluoromethyl （or monofluoromethyl） sulfones in 8ood yields. It was found that the relative reactivity of these sodium fluoroalkanesulfinates in the present reactions decreases in the followin8 order： CH2FSO2Na 〉 CF2HSO2Na 〉 CF3SO2Na.

关键词： difluoromethanesulfonylation fluoromethanesulfonylation arenediazonlum sodium sulfinate fluorine effect

Microelectrode arrays for monitoring neural activity in neural stem cells with modulation by glutamate in vitro

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Nanotechnology and Precision Engineering 2020年第2期3卷 69-74页

作者： Fei Gao Jinping Luo Yilin Song Enhui He Yu Zhang Guihua Xiao Xinxia Cai State key Laboratory of Transducer Technology Institute of ElectronicsChinese Academy of SciencesBeijing 100190PR China University of Chinese Academy of sciences Beijing 100049PR China Institute of Electronics Chinese Academy of Sciences

In this study, a 60-channel microelectrode array(MEA) was fabricated and used to monitor the neural spikes and local field potentials(LFPs) of neurons differentiated from rat neural stem cells in vitro. The neurons were grown on the MEA surface to detect neural signals. Glutamate(Glu) was used to modulate neural activity during experiments. To enhance detection performance, platinum nanoparticles were modified onto the microelectrode site surface. Glutamate stimulated neural spikes and LFPs were recorded using the MEA. The average spike amplitude was approximately 70 μV in the normal state. The spike amplitude increased by 29% from 70 μV to 90 μV with Glu modulation. The firing rate increased by 69% from 4.01 Hz to 6.8 Hz with Glu modulation. The LFP power increased from 326 μW in the normal state to 617 μW with Glu modulation in the 0–10 Hz frequency band. Data analysis shows that neural activity stimulated by Glu modulation was recorded experimentally at high temporal-spatial resolution. These results may provide a new neuron detection method, as well as further understanding of neural stem cell spike firing and associated mechanisms.

关键词： MEA Glutamate Spikes Neural stem cells In vitro

A new species of the Southeast Asian genus Opisthotropis (Serpentes Colubridae： Natricinae) from western Hunan, China

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Zoological Research 2017年第5期38卷 251-263页

作者： Jin-Long Ren Kai Wang Ke Jiang Peng Guo Jia-Tang Li cas key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation key Laboratory of Sichuan Province Chengdu Institute of Biology Chinese Academy of Sciences Chengdu Sichuan 610041 China Southeast Asia Biodiversity Research Institute Chinese Academy of Sciences Yezin Nay Pyi Taw 05282 Myanmar University of Chinese Academy of sciences Beijing 100049 China Sam Noble Oklahoma Museum of Natural History & Department of Biology University of Oklahoma Norman Oklahoma 73019 USA College of Life sciences and Food Engineering Yibin University Yibin Sichuan 644007 China

A new species of natricine snake of the Southeast Asian genus Opisthotropis GUnther, 1872 is described from western Hunan Province of China based on both mitochondrial DNA and morphological data. The new species is morphologically most similar and genetically most closely related to O. cheni Zhao, 1999 and O. latouchfi （Boulenger, 1899）, but possesses considerable genetic divergence （p- distance 5.1%-16.7%） and can be differentiated from all other congeners by a combination of the following morphological characters：（1） body size large （total length 514-586 mm） and strongly built; （2） dorsal scale rows 17 throughout, feebly keeled anteriorly and moderately keeled posteriorly; （3） ventral scales 147-152, subcaudal scales 54-62; （4） preocular absent, Ioreal elongated and touching orbit; （5） supralabials 8-9, fifth and sixth entering obit; （6） anterior temporals short, length 1.74-2.04 times longer than width; （7） maxillary teeth subequal, 28-30; （8） dorsal surface of head with distinct irregular yellow stripes and markings edged with ochre; （9） body with clear black and yellow longitudinal streaks, partly fused to several lighter patches or thicker stripes anteriorly; and （10） venter pale yellow, with asymmetric blackish speckles along outer margin. We present an updated diagnostic key to all members of the genus Opisthotropis, and recommendations on the ecological study for the group are provided.

关键词： Distribution Taxonomy OpisthotropisnovNatricinae Natural historyOpisthotropis zhaoermfi sp

Dynamic regulation of innate lymphoid cells in the mucosal immune system

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Cellular & Molecular Immunology 2021年第6期18卷 1387-1394页

作者： Fei Shao Dou Yu Pengyan Xia Shuo Wang cas key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina University of Chinese Academy of sciences BeijingChina Division of Life sciences of Medicine University of Science and Technology of ChinaHefeiChina Department of Immunology School of Basic Medical SciencesNHC Key Laboratory of Medical ImmunologyPeking UniversityBeijingChina

The mucosal immune system is considered a local immune system,a term that implies regional *** tissues are continually exposed to a wide range of *** regulation of mucosal immune cells is tightly associated with the progression of mucosal *** lymphoid cells(ILCs)are abundant in mucosal barriers and serve as first-line defenses against *** subtype changes and translocation of ILCs are accompanied by the pathologic processes of mucosal ***,we review the plasticity and circulation of ILCs in the mucosal immune system under physiological and pathological *** also discuss the signaling pathways involved in dynamic ILC changes and the related targets in mucosal diseases.

关键词： ILCs transdifferentiation translocation dynamic regulation mucosal immune system

Chen's Reagent: A Versatile Reagent for Trifluoromethylation,Difluoromethylenation, and Difluoroalkylation in Organic Synthesis

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Chinese Journal of Chemistry 2020年第2期38卷 202-212页

作者： Qiqiang Xie Jinbo Hu key Laboratory of Organofluorine Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of Sciences345 Ling-Ling RoadShanghai 200032China

Methyl fluorosulfonyldifluoroacetate(FSO2CF2CO2Me or MFSDA),often called"Chen's reagent",is commonly used to synthesize trifluoromethylated and difluoroalkylated *** important reagent was initially developed as an efficient trifluoromethylating agent by Professor Qing-Yun Chen and co-workers at Shanghai Institute of Organic Chemistry,Chinese Academy of sciences in 1989.

关键词： compounds. reagent Reagent

Fluoro-Hydroxylation of gem-Difluoroalkenes:Synthesis of 18O-labeled α-CF3 Alcohols

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Chinese Journal of Chemistry 2018年第12期36卷 1202-1208页

作者： Jingyu Hu Yide Yang Zhengzhao Lou Chuanfa Ni Jinbo Hu key Laboratory of Organofluorine Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryUniversity of Chinese Academy of SciencesChinese Academy of Sciences345 Lingling LuShanghai 200032China School of Physical science and Technology ShanghaiTech University100 Haike RoadShanghai 201210China

In this paper, a fluoro‐hydroxylation of gem ‐difluoroalkenes is demonstrated. This protocol uses sequential electrophilic fluorination with Selectfluor and nucleophilic hydroxylation with H 2 18 O, which can be used to prepare 18 O‐labeled α‐CF 3 alcohols from gem ‐difluorostyrenes. The reaction is typically carried out at room temperature within 4 h in good to excellent yields. Other nucleophiles (besides H 2 18 O), such as alcohols, carboxylic acid, acetonitrile and N,N ‐dimethylformamide, are also suitable for this difunctionalization of gem ‐difluoroalkenes.

关键词： gem‐difluoroalkene difunctionalization electrophilic fluorination 18O‐labeling trifluoromethyl carbinol

Characteristics of replication and pathogenicity of SARS-CoV-2 Alpha and Delta isolates

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Virologica Sinica 2022年第6期37卷 804-812页

作者： Xiao-Li Feng Dandan Yu Mi Zhang Xiaohong Li Qing-Cui Zou Wentai Ma Jian-Bao Han Ling Xu Cuixian Yang Wang Qu Zhong-Hua Deng Junyi Long Yanghaopeng Long Mingkun Li Yong-Gang Yao Xing-Qi Dong Jianxiong Zeng Ming-Hua Li Kunming National High-level Biosafety Research Center for Non-Human Primates Center for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunming650107China key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of sciences and KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesKunming Institute of ZoologyChinese Academy of SciencesKunming650201China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunming650107China Department of Infectious Diseases Yunnan Provincial Infectious Diseases HospitalKunming650301China key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciencesand China National Center for BioinformationBeijing100101China University of Chinese Academy of sciences Beijing100049China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming650201China Kunming College of Life science University of Chinese Academy of SciencesKunming650204China Yunnan key Laboratory of Biodiversity Information Kunming Institute of ZoologyChinese Academy of SciencesKunming650201China

The continuously arising of SARS-CoV-2 variants has been posting a great threat to public health safety globally,from B.1.17(Alpha), B.1.351(Beta), P.1(Gamma), B.1.617.2(Delta) to B.1.1.529(Omicron). The emerging or reemerging of the SARS-CoV-2 variants of concern is calling for the constant monitoring of their epidemics,pathogenicity and immune escape. In this study, we aimed to characterize replication and pathogenicity of the Alpha and Delta variant strains isolated from patients infected in Laos. The amino acid mutations within the spike fragment of the isolates were determined via sequencing. The more efficient replication of the Alpha and Delta isolates was documented than the prototyped SARS-CoV-2 in Calu-3 and Caco-2 cells, while such features were not observed in Huh-7, Vero E6 and HPA-3 cells. We utilized both animal models of human ACE2(hACE2)transgenic mice and hamsters to evaluate the pathogenesis of the isolates. The Alpha and Delta can replicate well in multiple organs and cause moderate to severe lung pathology in these animals. In conclusion, the spike protein of the isolated Alpha and Delta variant strains was characterized, and the replication and pathogenicity of the strains in the cells and animal models were also evaluated.

关键词： SARS-CoV-2 Variants of concern(VOC) Alpha Delta hACE2 transgenic mice Hamster