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Deciphering the cellular heterogeneity of the insect brainwith single-cell RNA sequencing

Insect Science 2024年第2期31卷 314-327页

作者： Xiaofei Wang Yifan Zhai Hao Zheng Institute of Plant Protection Shandong Academy of Agricultural SciencesJinanChina College of Food Science and Nutritional En-gineering China Agricultural UniversityBeijingChina Key Laboratory of natural Enemies Insects Ministry of Agriculture and RuralAffairsJinanChina Shandong Provincial Engineering Technology Research Center on Biocontrol of Crops Diseases and In-sectPests JinanChina

Insects show highly complicated adaptive and sophisticated behaviors, includ-ing spatial orientation skills, learning ability, and social interaction. These behaviors arecontrolled by the insect brain, the central part of the nervous system. The tiny insect brainconsists of millions of highly differentiated and interconnected cells forming a complexnetwork. Decades of research has gone into an understanding of which parts of the insectbrain possess particular behaviors, but exactly how they modulate these functional conse-quences needs to be clarified. Detailed description of the brain and behavior is required todecipher the complexity of cell types, as well as their connectivity and function. Single-cell RNA-sequencing (scRNA-seq) has emerged recently as a breakthrough technologyto understand the transcriptome at cellular resolution. With scRNA-seq, it is possible touncover the cellular heterogeneity of brain cells and elucidate their specific functions andstate. In this review, we first review the basic structure of insect brains and the links toinsect behaviors mainly focusing on learning and memory. Then the scRNA applicationson insect brains are introduced by representative studies. Single-cell RNA-seq has allowedresearchers to classify cell subpopulations within different insect brain regions, pinpointsingle-cell developmental trajectories, and identify gene regulatory networks. These devel-opments empower the advances in neuroscience and shed light on the intricate problemsin understanding insect brain functions and behaviors.

关键词： behavior brain cell type insect single-cell RNA sequencing

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An experimental and theoretical study on photoluminescence of Ce^(3+)-doped ZnS quantum dots and their application in Aspergillus oryzae

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Journal of Rare Earths 2024年第6期42卷 1020-1028,I0001页

作者： Yan Lei Guangzhi Zhang Yitong Yao Gaiqin Zhu Zhong Chen Jiangrong Dan Hua Liu Shuwang Duo School of Materials and Mechanical and Electrical EngineeringJiangxi Science and Technology Normal University Nanchang330013China

ZnS and ZnS:3.125%Ce^(3+)(ZSC)quantum dots(QDs)were synthesized by a low-temperature solid-phase *** crystal structure,photoluminescence(PL)properties of ZSC QDs,and their effects on the growth and kojic acid production of Aspergillus oryzae(***)were *** is found that both QDs have a cubic blend structure with the average particle size ranging of 2.75-6.14 ***^(3+)doping not only increases the band gap of ZnS QDs from 3.43 to 3.48 eV,but also increases the PL intensity by about 2.23 times,which is due to the fact that the electron carrier concentration is improved,and then the rate of radiative recombination is *** integral emission intensity,biomass,extracellular proteins yield,and kojic acid production of *** cultured with ZSC(ZnS)QDs are about 4.31(3.23),1.61(1.20),1.12(1.09),and 2.99(1.68)times higher than that of medium without QDs,respectively.

关键词： Zns Quantum dots Doping First-principles calculations Aspergllus oryzae Rare earths

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A study on the application of diffuse axonal multi-axis general evaluation for braininjury assessment in small overlap barrier crash test

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Chinese Journal of Traumatology 2024年第4期27卷 200-210页

作者： Zhi Fu Yi Chang Tao Xiong Wen-Kai Gao Kui Li Yu Liu china Automotive Engineering Research Institute Co. Ltd.Chongqing401122China Chongqing University of Technology Chongqing400054China Chongqing Key Laboratory of Vehicle Crash/Bio-Impact and Traffic Safety Chongqing400042China College of Medical Informatics Chongqing Medical UniversityChongqing400016China

Purpose:Head injury criterion(HIC)companied by a rotation-based metric was widely believed to behelpful for head injury prediction in road traffic ***,the Euro-New Car AssessmentProgram utilized a newly developed metric called diffuse axonal multi-axis general evaluation(DAMAGE)to explain test device for human occupant restraint(THOR)head injury,which demonstratedexcellent ability in capturing concussions and diffuse axonal ***,there is still a lack ofcomprehensive understanding regarding the effectiveness of using DAMAGE for Hybrid III 50thpercentile male dummy(H50th)head injury *** objective of this study is to determinewhether the DAMAGE could capture the risk of H50th brain injury during small overlap barrier ***:To achieve this objective,a total of 24 vehicle crash loading curves were collected as input datafor the multi-body *** commercially available mathematical dynamic models,namelyH50th and THOR,were utilized to investigate the differences in head injury ***,adecision method known as simple additive weighting was employed to establish a comprehensive braininjury metric by incorporating the weighted HIC and either DAMAGE or brain injury ***,35 sets of vehicle crash test data were used to analyze these brain injury ***:The rotational displacement of the THOR head is significantly greater than that of the *** maximum linear and rotational head accelerations experienced by H50th and THOR modelswere(544.6±341.7)m/s^(2),(2468.2±1309.4)rad/s^(2) and(715.2±332.8)m/s^(2),(3778.7±1660.6)rad/s^(2),*** the same loading condition during small overlap barrier(SOB)tests,THOR exhibits ahigher risk of head injury compared to the H50th *** was observed that the overall head injuryresponse during the small overlap left test condition is greater than that during the small overlap ***,an equation was formulated to establis

关键词： Diffuse axonal multi-axis general evaluation Head injury criterion Head injury assessment Small overlap barrier Biomechanical response

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Recursive calibration for a lithium iron phosphate battery for electric vehicles using extended Kalman filtering

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第11期12卷 818-825页

作者： Xiao-song HU Feng-chun SUN Xi-ming CHENG national Engineering Laboratory for Electric Vehicles Department of Mechanical EngineeringBeijing Institute of Technology Beijing 100081 China

In this paper,an efficient model structure composed of a second-order resistance-capacitance network and a simply analytical open circuit voltage versus state of charge(SOC) map is applied to characterize the voltage behavior of a lithium iron phosphate battery for electric vehicles(EVs).As a result,the overpotentials of the battery can be depicted using a second-order circuit network and the model parameterization can be realized under any battery loading profile,without a special characterization *** order to ensure good robustness,extended Kalman filtering is adopted to recursively implement the calibration *** linearization involved in the calibration algorithm is realized through recurrent derivatives in a recursive *** results show that the recursively calibrated battery model can accurately delineate the battery voltage behavior under two different transient power operating conditions.A comparison with a first-order model indicates that the recursively calibrated second-order model has a comparable accuracy in a major part of the battery SOC range and a better performance when the SOC is relatively low.

关键词： Model calibration Lithium iron phosphate battery Electric vehicle (EV) Extended Kalman filtering

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Application of grey relational analysis in sheet metal forming for multi-response quality characteristics

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 805-811页

作者： XIE Yan-min YU Hu-ping CHEN Jun RUAN Xue-yu national Die ＆ Mold CAD Engineering Research Center Shanghai Jiao Tong University Shanghai 200030 China

The theory of grey systems is a new technique for performing prediction, relational analysis and decision making in many areas. In this paper, the use of grey relational analysis for optimizing the square hole flanging process parameters with considerations of the multiple response (the average flanging height, regular flanging and maximum strain) is introduced. Various flanging parameters, such as the blank inner radius rb, blank inner width B0, are considered. An orthogonal array is used for the experimental design. Multiple response values are obtained using finite element analysis (FEA). Optimal process parameters are determined by the grey relational grade obtained from the grey relational analysis for multi-performance characteristics (flanging height, regular flanging and maximum strain). Analysis of variance (ANOVA) for the grey relational grade is implemented. The results showed good agreement with the experiment result. Grey relational analysis can be applied in multiple response optimi-zation designs.

关键词： Grey relational analysis Flanging Multiple response Optimization design

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Effect of suction change on water content and total volume of an expansive clay

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 699-706页

作者： ZHAN Liang-tong CHEN Ping NG C.W.W. ~ Department of Civil Engineering Zhejiang University Hangzhou 310027 China Department of Civil Engineering Zhejiang Sci- Tech University Hangzhou 310018 China Department of Civil Engineering Hong Kong University of Science and Technology Hong Kong China

A laboratory study was carried out on both natural and compacted specimens to investigate the complex soil-water interaction in an unsaturated expansive clay. The laboratory study includes the measurement of soil-water characteristic curves, 1D free swelling tests, measurement of swelling pressure and shrinkage tests. The test results revealed that the air-entry value of the natural specimen was quite low due to cracks and fissures present. The hydraulic hysteresis of the natural specimen was relatively insignificant as compared with the compacted specimen. Within a suction range 0 to 500 kPa, a bilinear relationship between free swelling strain (or swelling pressure) and initial soil suction was observed for both the natural and compacted specimens. As a result of over-consolidation and secondary structures such as cementation and cracks, the natural specimens exhibited significant lower swelling (or swelling pressure) than the compacted specimen. The change of matric suction exerts a more significant effect on the water phase than on the soil skeleton for this expansive clay.

关键词： Expansive soil Water content Suction Swelling Shrinkage

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Particle contamination, the disruption of electronic connectors in the signal transmission system

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第3期8卷 361-369页

作者： ZHANG Ji-gao Research Lab of Electric Contacts Beijing University of Posts ＆ Telecommunications Beijing 100876 China

Particle pollution in air, also sometimes known as fine dust contamination, may cause electric contact failure. Recent research further proved that the fine particle is becoming a major disruption of the electronic connectors in signal transmission system. This paper specifies the connector contact in mobile phone application. To study the contact failure of mobile phone, a series of inspections and analytical research methods are introduced. Special features that cause the contact failure are summarized. Particle accumulation is the main problem; organic material such as lactates from sweat of the human body may act as adhesives to stick the separate particles together and make them adhere on the contact surface; chemical properties of dust cause serious local corrosion. The corrosion products may trap the particles and firmly attach on the contact surface; micro motion frequently occurs at the contact interface. Hard particle can be embedded into the surface, and soft particle could be squeezed and inserted into the contact; silicon compounds in dust play the most important role in forming high resistance regions that lead to failure; deposition of particles depends on the amount of materials, static electricity attracting force and gravity force applied on the particles. Current dust test can hardly reflect the serious contact failure. It is difficult to simulate the complexity of contact failure caused by particle contamination. Thus alternative ways of simulation experiment and improvement of contact reliability are proposed.

关键词： Dust Particle contamination Micro motion Corrosion

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Enhanced control of DFIG-used back-to-back PWM VSC under unbalanced grid voltage conditions

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第8期8卷 1330-1339页

作者： HU Jia-bing HE Yi-kang NIAN Heng School of Electrical Engineering Zhejiang University Hangzhou 310027 China

This paper presents a unified positive-and negative-sequence dual-dq dynamic model of wind-turbine driven doubly-fed induction generator(DFIG) under unbalanced grid voltage conditions. Strategies for enhanced control and operation of a DFIG-used back-to-back(BTB) PWM voltage source converter(VSC) are proposed. The modified control design for the grid-side converter in the stationary αβ frames diminishes the amplitude of DC-link voltage ripples of twice the grid frequency,and the two proposed control targets for the rotor-side converter are alternatively achieved,which,as a result,improve the fault-ride through(FRT) capability of the DFIG based wind power generation systems during unbalanced network supply. A complete unbalanced control scheme with both grid-and rotor-side converters included is designed. Finally,simulation was carried out on a 1.5 MW wind-turbine driven DFIG system and the validity of the developed unified model and the feasibility of the proposed control strategies are all confirmed by the simulated results.

关键词： Unbalanced grid voltage Doubly-fed induction generator (DFIG) Back-to-back (BTB) Fault-ride through (FRT) Voltage source converter (VSC)

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Fire's effect on chloride ingress related durability of concrete structure

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 675-681页

作者： JIN Wei-liang ZHANG Yi Structural Engineering Institute Zhejiang University Hangzhou 310027 China

This paper describes the effects of fire on durability of reinforced concrete structures, and points out that fire not only damages the chemical composition and physical structure of concrete by high temperature, but also leads to an additional risk due to the generation of polyvinyl chloride (PVC) combustion gases. A mathematical model is proposed to calculate chloride ingress profiles in fire damaged concrete, so as to explore the service life prediction of the structure. Rapid Chloride Migration (RCM) test was carried out to determine the chloride diffusion coefficients for the application of the mathematical model. Finally, the detected results of a reported case testified to the validity of the mathematical model.

关键词： Fire High temperature Polyvinyl chloride (PVC) Mathematical model Rapid Chloride Migration (RCM)

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Study of engine noise based on independent component analysis

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 772-777页

作者： HAO Zhi-yong JIN Yan YANG Chen School of Mechanical and Energy Engineering Zhejiang University Hangzhou 310027 China

Independent component analysis was applied to analyze the acoustic signals from diesel engine. First the basic prin-ciple of independent component analysis (ICA) was reviewed. Diesel engine acoustic signal was decomposed into several inde-pendent components (ICs); Fourier transform and continuous wavelet transform (CWT) were applied to analyze the independent components. Different noise sources of the diesel engine were separated, based on the characteristics of different component in time-frequency domain.

关键词： Acoustic signals Independent component analysis (ICA) Wavelet transform Noise source identification

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