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Cuproptosis mediates copper-induced testicular spermatogenic cell death

Asian Journal of Andrology 2024年第3期26卷 295-301页

作者： Jing-Yi Zhang Xu-Jun Yu Jun-Jun Li Yao Xiao Guang-Sen Li Fang Yang Liang Dong Chengdu University of Traditional Chinese Medicine Chengdu 611137China Chengdu Fifth People’s Hospital The Fifth People’s Hospital of Chengdu University of Traditional Chinese MedicineChengdu 611130China TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072China Department of Surgery The Reproductive and Women-Children HospitalChengdu University of Traditional Chinese MedicineChengdu 610041China

Cuproptosis,a novel mechanism of programmed cell death,has not been fully explored in the context of spermatogenic *** investigated the potential involvement of cuproptosis in spermatogenic cell death using a mouse model of copper *** male Institute of Cancer Research(ICR)mice were randomly divided into four groups that received daily oral gavage withsodium chloride(control)or copper sulfate(CuSO_(4))at 50 mg kg^(−1),100 mg kg^(−1),or 200 mg kg^(−1),for 42 consecutive *** to copper overload exhibited a disruption in copper ***,significant upregulated expression of keycuproptosis factors was accompanied by a significant rise in the rates of testicular tissue cell *** revealed the presence of ferredoxin 1(Fdx1)in Sertoli cells,Leydig cells,and spermatogenic cells at various stages oftesticular development,and the Fdx1-positive staining area was significantly increased in copper-overloaded *** and decreased adenosine triphosphate levels were also observed,further implicating mitochondrial damage *** analyses revealed pathological lesions and blood−testis barrier destruction in the testicular tissue,accompaniedby decreased sperm concentration and motility,in copper-overloaded *** summary,our results indicate that copper-overloadedmice exhibit copper homeostasis disorder in the testicular tissue and that cuproptosis participates in spermatogenic cell *** findings provide novel insights into the pathogenic mechanisms underlying spermatogenic cell death and provide initialexperimental evidence for the occurrence of cuproptosis in the testis.

关键词： blood−testis barrier cuproptosis ferredoxin 1 spermatogenic cell death

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Is there hybridization between 2 species of the same genus in sympatry?——The genetic relationships between Anoplophora glabripennis,Anoplophora chinensis,and putative hybrids

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Insect science 2024年第2期31卷 633-645页

作者： Haiwen Qin Huachao Xu Arnaud Capron Ilga Porth Mingming Cui Melody A.Keena Xiaofang Deng Juan Shi Richard C.Hamelin Sino-French Joint Laboratory for Invasive Forest Pests in Eurasia Department of ForestBejing Forestry UniversityBejingChina Department of Forest and Conservation sciences The University of British ColumbiaVancouverCanada College of Forestry and Biotechnology Zhejiang A&F UniversityHangzhouChina Department of Wood and Forest sciences Laval UniversityQuebecCanada 5 Department of AgricultureNorthern Research StationUSDA Forest ServiceHamdenConnecticutUSA Changchun Landscape Plant Conservation Station Bureau of Forestry and Landscaping of ChangchunChangchunChina

Anoplophora glabripennis(Asian longhorn beetle,ALB)and Anoplophora chinensis(Citrus longhorn beetle,CLB)are native forest pests in China;they have become important international quarantine *** are found using the same Salix aureopendula host tree of Cixi,Zhejiang province,*** this host tree,we collected additional beetles that appeared to be morphologically intermediate between ALB and *** using a stereoscope,we observed that there were several bumps on the base of the elytra,which was inconsistent with ALB,which typically has a smooth elytral base,but was more like CLB,which has numerous short tubercles on the elytral *** their sympatry and intermediate morphology,we hypothesized that these may represent ALB×CLB *** studied the genomic profiles for 46 samples(ALB,CLB,and putative hybrids)using genotyping-by-sequencing(GBS)providing a reduced representation of the entire *** principal component analyses on the 163 GBS-derived single nucleotide polymorphism data,we found putative hybrids tightly clustered with ALB,but genetically distinct from the CLB ***,our initial hybrid hypothesis was not supported by genomic ***,while mating experiments between adult ALB and CLB were successful in 4 separate years(2017,2018,2020,and 2021),and oviposition behavior was observed,no progeny was *** employed population genomic analysis and biological hybridization experiments,we conclude that the putative hybrids represent newly discovered morphological variants within *** approach further confirmed the advantage of genome-wide information for Anoplophora species assignment in certain ambiguous classification cases.

关键词： Anoplophora chinensis Anoplophora glabripenni sg enotyping-bysequencing invasive pest mating putative hybrid

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Properties of collective flow and pion production in intermediate-energy heavy-ion collisions with a relativistic quantum molecular dynamics model

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Nuclear science and Techniques 2024年第1期35卷 155-169页

作者： Si-Na Wei Zhao-Qing Feng School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510640China

The relativistic mean-field approach was implemented in the Lanzhou quantum molecular dynamics transport model(***). Using the ***, the properties of collective flow and pion production were investigated systematically for nuclear reactions with various isospin asymmetries. The directed and elliptic flows of the *** are able to describe the experimental data of STAR Collaboration. The directed flow difference between free neutrons and protons was associated with the stiffness of the symmetry energy, that is, a softer symmetry energy led to a larger flow difference. For various collision energies, the ratio between the π^(-) and π^(+) yields increased with a decrease in the slope parameter of the symmetry energy. When the collision energy was 270 MeV/nucleon, the single ratio of the pion transverse momentum spectra also increased with decreasing slope parameter of the symmetry energy in both nearly symmetric and neutron-rich ***, it is difficult to constrain the stiffness of the symmetry energy with the double ratio because of the lack of threshold energy correction on the pion production.

关键词： Heavy-ion collision Collective flow Pion production Symmetry energy Relativistic mean field

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3D bioprinting of collagen-based materials for oral medicine

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Collagen and Leather 2024年第1期5卷 28-46页

作者： Bosen Yang Hai Liu Linli Jiang Yiwei Zeng Yiyuan Han Chuanlu Sha Xin Xie Hui Li Jiajing Zhou Wei Lin College of Biomass science and Engineering Key Laboratory of Leather Chemistry and Engineering of Ministry of EducationNational Engineering Laboratory for Clean Technology of Leather ManufactureSichuan UniversityChengdu 610065China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesDepartment of Oral and Maxillofacial SurgeryWest China Hospital of StomatologySichuan UniversityChengdu 610065China Laboratory for Biomaterials and Drug Delivery Department of AnesthesiologyDivision of Critical Care MedicineBoston Children's HospitalHarvard Medical SchoolBostonMA 02115USA

Oral diseases have emerged as one of the leading public health challenges *** the existing clinical modalities for restoration of dental tissue loss and craniomaxillofacial injuries can achieve satisfactory therapeutic results,they cannot fully restore the original complex anatomical structure and physiological function of the tissue.3D printing of biological tissues has gained growing interest in the field of oral medicine with the ability to control the bioink component and printing structure for spatially heterogeneous repairing constructs,holding enormous promise for the precise treatment of oral ***,collagen-based materials have been recognized as promising biogenic bioinks for the regeneration of several tissues with high cell-activating and biocompatible *** this review,we summarize 3D printing methods for collagen-based biomaterials and their ***,we highlight the animal sources of collagen and their characteristics,as well as the methods of collagen ***,this review provides an overview of the 3D bioprinting technology for the regeneration of the pulpal nerve and blood vessels,cartilage,and periodontal *** envision that this technique opens up immense opportunities over the conventional ones,with high replicability and customized function,which can ultimately promote effective oral tissue regeneration.

关键词： 3D bioprinting Oral medicine Tissue engineering Collagen Scaffold materials Bioink Hydrogel

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RNF187 governs the maintenance of mouse GC-2 cell development by facilitating histone H3 ubiquitination at K57/80

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Asian Journal of Andrology 2024年第3期26卷 272-281页

作者： Bing-Ya Xu Xiang-Ling Yu Wen-Xin Gao Ting-Ting Gao Hao-Yue Hu Tian-Tian Wu Cong Shen Xiao-Yan Huang Bo Zheng Yi-Bo Wu Human Reproductive and Genetic Center Affiliated Hospital of Jiangnan UniversityWuxi 214122China State Key Laboratory of Reproductive Medicine Department of Histology and EmbryologySchool of Basic Medical SciencesNanjing Medical UniversityNanjing 211166China Changzhou Maternal and Child Health Care Hospital Changzhou Medical CenterNanjing Medical UniversityChangzhou 213000China State Key Laboratory of Reproductive Medicine Center for Reproduction and GeneticsSuzhou Municipal HospitalThe Affiliated Suzhou Hospital of Nanjing Medical UniversityGusu SchoolNanjing Medical UniversitySuzhou 215002China

RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer ***,it remains unclear whether RNF187 exhibits comparable functionality in the development of germline *** investigate thepotential involvement of RNF187 in germ cell development,we conducted interference and overexpression assays using GC-2 cells,a mouse spermatocyte-derived cell *** findings reveal that the interaction between RNF187 and histone H3 increases theviability,proliferation,and migratory capacity of GC-2 ***,we provide evidence demonstrating that RNF187 interactswith H3 and mediates the ubiquitination of H3 at lysine 57(K57)or lysine 80(K80),directly or indirectly resulting in increasedcellular *** is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediatinghistone H3 ubiquitination,thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of *** discoveries provide a new theoretical foundation for further comprehensive investigations into the functionof RNF187 in the reproductive system.

关键词： GC-2 cell histone H3 RNF187 ubiquitination

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Therapeutic genome editing of an aberrant splice site inβ-thalassemia by CRISPR/Cas9 with multiple sgRNAs

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Genes & Diseases 2024年第1期11卷 15-18页

作者： Fei Yang Shuqian Xu Chao Huang Zhiqiang Shao Yuefen Hu Yang Yang Yongrong Lai Qing Ke Dan Liang Yuxuan Wu Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life SciencesEast China Normal UniversityShanghai 200241China Department of Obstetrics and Gynecology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract(Anhui Medical University) HefeiAnhui 230032China Department of Hematology Qilu HospitalCheeloo College of MedicineShandong UniversityJinanShandong 250012China BRLMedicine Inc. Shanghai 201108China Department of Hematology The First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530000China Department of Neurology The First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310003China

Genetic mutations cause aberrant splicing,one of the important molecular mechanisms in human ***2-654,a mutation causing aberrant splicing ofβ-globin premRNA and contributing toβ-globin deficiency,is one of the most common diseases in Southeast Asia and China."In our previous work,we found a TTTV protospacer adjacent motif(PAM)by the Cas12a system and the editing efficiency of Ivs2-654 C>T achieving 76.7%.

关键词： CRISPR/Cas9 China thalassemia

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Optimizing the Qusai-static Folding and Deploying of Thin-Walled Tube Flexure Hinges with Double Slots

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Chinese Journal of Mechanical Engineering 2014年第2期27卷 279-286页

作者： YANG Hui DENG Zongquan LIU Rongqiang WANG Yan GUO Hongwei State Key Laboratory of Robotics and System Harbin Institute of Technology

The thin-walled tube flexure（TWTF） hinges have important potential application value in the deployment mechanisms of satellite and solar array, but the optimal design of the TWTF hinges haven＇t been completely solved, which restricts their applications. An optimal design method for the qusai-static folding and deploying of TWTF hinges with double slots is presented based on the response surface theory. Firstly, the full factorial method is employed to design of the experiments. Then, the finite element models of the TWTF hinges with double slots are constructed to simulate the qusai-static folding and deploying non-linear analysis. What＇s more, the mathematical model of the TWTF flexure hinge quasi-static folding and deploying properties are derived by the response surface method. Considering of small mass and high stability, the peak moment of quasi-static folding and deploying as well as the lightless are set as the objectives to get the optimal performances. The relative errors of the objectives between the optimal design results and the FE analysis results are less than 7%, which demonstrates the precision of the surrogate models. Lastly, the parameter study shows that both the slots length and the slots width both have significant effects to the peak moment of quasi-static folding and deploying of TWTF hinges with double slots. However, the maximum Mises stress of quasi-static folding is more sensitive to the slots length than the slots width. The proposed research can be applied to optimize other thin-walled flexure hinges under quasi-static folding and deploying, which is of great importance to design of flexure hinges with high stability and low stress.

关键词： design optimization quasi-static folding and deploying flexure hinges thin-walled tube response surface method,numerical simulation

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Antioxidative effect of propofol during cardiopulmonary bypass in adults

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Acta Pharmacologica Sinica 2004年第3期25卷 80-86页

作者： Shi-hai ZHANG2,Shou-yong WANG,Shang-long YAO Department of Anesthesiology,Union Hospital,Tongji MedicalCollege,Huazhong Universityof science & Technology,Wuhan 430022,China Department of Anesthesiology Union Hospital Tongji Medical College Huazhong University of Science & Technology Wuhan 430022

AIM: To investigate the antioxidative potential of propofol (an intravenous anesthetic with a chemical structure similarto phenol-basedfree radicalscavengers suchas vitaminE) during cardiopulmonary bypass(CPB). METHODS: Thirty adult patients referred for elective cardiac procedure with CPB were included and randomly allocated to a propofol group or a control group. Patients in the propofol group received propofol (0.1 mg·kg-1·min-1) intrave- nously for anesthesia maintenance, whereas those allocated to the control group received fentanyl 10 μg/kg intrave- nously and inhaled enflurane (1 %-1.5 %). Blood samples were collected at 7 time points: before the start of CPB, at 30 and 60 min of CPB, at the conclusion of CPB, 10 min after the administration of protamine, and 12 and 24 h after the cessation of CPB. Plasma levels of free F2-isoprostanes (sensitive markers of free radicals production) and complement C5a were determined by mass-spectrometricassay and enzyme immunoassay, respectively. Neu- trophil adhesion to endothelial cells was observed at ×200 magnificationunder a light microscope. RESULTS: Levels of F2-isoprostanes, complement C5a and neutrophil adhesion rate increased significantly during and after CPB in both groups. There were significantly higher levels of F2-isoprostanes, C5a, and more neutrophils adhering to endothelial cells in the control group than those in the propofol group, respectively. CONCLUSION: Cardio- pulmonary bypass is associated with a great production of damaging free radicals. Propofol may be beneficial both as an anesthetic and as a potent free radical scavenger in patients presenting pathologies associated with free radical reactions during CPB.

关键词： propofol cardiopulmonary bypass free radicals F2-isoprostanes complement 5a

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Experiment and Numerical Simulation of Free Water Jet by a Central-body Nozzle

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Chinese Journal of Mechanical Engineering 2010年第6期23卷 797-804页

作者： YANG Minguan ZHANG Feng KANG Can GAO Bo School of Energy and Power Engineering Jiangsu University Zhenjiang 212013 China

The recent research about cavitation jet mainly focuses on the organ-pipe nozzle and triangular nozzle. The research content mainly includes the optimized design about the structure of nozzles, the observation and flow analysis about the cavitation jet in the water, and the theory of rock attacked by the cavitation jet, while the energy characteristic of the free jet is not studied yet. In China, the research about the central-body nozzle is almost empty. For the purpose of studying the energy characteristic and the structure of free water jet discharged from central-body nozzle, an experiment with phase Doppler particle anemometry（PDPA） technology is carried out to measure the free water jet flow, which is produced by a central-body nozzle under the jet pressure of 15 MPa. While five sections with different axial distances from the nozzle outlet are selected for data process and analysis, the axial and radial velocity and the droplets of the particle size are studied. Meanwhile, numerical calculation of corresponding flow field is conducted by using volume of fluid（VOF） multiphase model, and the jet flow feature is discussed. The experimental and calculating results show that the axial velocity of high speed jet flow dissipates slowly in the air, and the core area and diffused area are discovered. The diameter of droplet in the core area is small, and jet energy is concentrated, while in the diffusion area, water is mingled with ambient air and radial velocity is relatively large. Obvious low-pressure area exists behind the central body and potential cavitation may occur in that area. The proposed research reveals the energy characteristic of free jet discharged from central-body nozzle, provides the theoretical basis for preestimating erosion feature of the central-body nozzle and also the theoretical foundation for revealing the mechanism of erosion.

关键词： central-body nozzle free water jet phase Doppler particle anemometry(PDPA) energy characteristic VOF model

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Effect of Manufacturing Errors on Static Characteristics of Externally Pressurized Spherical Air Bearings

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Chinese Journal of Mechanical Engineering 2009年第6期22卷 896-902页

作者： REN Di WANG Zuwen YANG Qingjun BAO Gang School of Mechanical and Electrical Engineering Harbin Institute of Technology Harbin 150001 China Transportation and Logistics Engineering College Dalian Maritime University Dalian 116026 China

Externally pressurized spherical air bearings are the key component of the three-axis air bearing table, and the manufacturing errors of the bearing affects the performance of the air bearing table. However, the manufacturing errors are unavoidable, and the pursuit to enhance the manufacturing accuracy will increase the cost greatly. In order to provide some theoretical guideline for the tolerance choice in the design of the externally pressurized spherical air bearings with inherent compensation, the effects of several manufacturing errors on the static characteristics of the air bearing are studied. Due to the complex geometry of the computational domain, an unstructured meshing technology is used for mesh generation. A finite-volume method is adopted to discretize the three-dimensional steady-state compressible Navier-Stokes equations. A modified SIMPLE algorithm which is suitable for compressible flows is applied to solve the discretized governing equations. The effects of the dimension error and the roundness error of the ball head and the ball socket on the static characteristics are investigated. The investigation result shows that the positive dimension error and the oblate spheroid-type roundness error of the ball head as well as the negative dimension error and the prolate spheroid-type roundness error of the ball socket can improve the bearing capacity and static stiffness of the air bearings by reducing the mass flow. The calculation method proposed in this paper fits well for the general principle, which can be extended to the characteristics analysis of other air bearings.

关键词： externally pressurized spherical air bearings manufacturing errors bearing capacity static stiffness mass flow

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