Is there hybridization between 2 species of the same genus in sympatry?——The genetic relationships between Anoplophora glabripennis,Anoplophora chinensis,and putative hybrids

作     者：Haiwen Qin Huachao Xu Arnaud Capron Ilga Porth Mingming Cui Melody A.Keena Xiaofang Deng Juan Shi Richard C.Hamelin 

作者机构：Sino-French Joint Laboratory for Invasive Forest Pests in EurasiaDepartment of ForestBejing Forestry UniversityBejingChina Department of Forest and Conservation SciencesThe University of British ColumbiaVancouverCanada College of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouChina Department of Wood and Forest SciencesLaval UniversityQuebecCanada 5 Department of AgricultureNorthern Research StationUSDA Forest ServiceHamdenConnecticutUSA Changchun Landscape Plant Conservation StationBureau of Forestry and Landscaping of ChangchunChangchunChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2024年第31卷第2期

页      面：633-645页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the Fundamental Research Funds for the Central Universities(No.QNTD202304) National Natural Science Foundation ofChina(No.32171794) 

主　　题：Anoplophora chinensis Anoplophora glabripenni sg enotyping-bysequencing invasive pest mating putative hybrid 

摘      要：Anoplophora glabripennis(Asian longhorn beetle,ALB)and Anoplophora chinensis(Citrus longhorn beetle,CLB)are native forest pests in China;they have become important international quarantine *** are found using the same Salix aureopendula host tree of Cixi,Zhejiang province,*** this host tree,we collected additional beetles that appeared to be morphologically intermediate between ALB and *** using a stereoscope,we observed that there were several bumps on the base of the elytra,which was inconsistent with ALB,which typically has a smooth elytral base,but was more like CLB,which has numerous short tubercles on the elytral *** their sympatry and intermediate morphology,we hypothesized that these may represent ALB×CLB *** studied the genomic profiles for 46 samples(ALB,CLB,and putative hybrids)using genotyping-by-sequencing(GBS)providing a reduced representation of the entire *** principal component analyses on the 163 GBS-derived single nucleotide polymorphism data,we found putative hybrids tightly clustered with ALB,but genetically distinct from the CLB ***,our initial hybrid hypothesis was not supported by genomic ***,while mating experiments between adult ALB and CLB were successful in 4 separate years(2017,2018,2020,and 2021),and oviposition behavior was observed,no progeny was *** employed population genomic analysis and biological hybridization experiments,we conclude that the putative hybrids represent newly discovered morphological variants within *** approach further confirmed the advantage of genome-wide information for Anoplophora species assignment in certain ambiguous classification cases.