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Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in pakistan

Virologica Sinica 2018年第5期33卷 410-417页

作者： Ali Zohaib Muhammad Saqib Muhammad Ammar Athar Jing Chen Awais-ur-Rahman Sial Saeed Khan Zeeshan Taj Halima Sadia Usman Tahir Muhammad Haleem Tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal Ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail Sajid Victor Max Corman Bing Yan Zheng-Li Shi CAS key Laboratory of Special Pathogens and biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of Sciences Beijing 100049China Faculty of Veterinary Medicine University of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid Biopsy College of PharmacyHenan UniversityHenan 475004China Department of Clinical Studies Faculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health Science Pakistan Karachi 74200 Department of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD) Lahore 54000Pakistan College of Veterinary and Animal Sciences The Islamia University of BahawalpurBahawalpur 63100Pakistan State key Laboratory of Agricultural Mlcroblology Huazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health Science Pakistan Jamshoro 76090 Section of Epidemiology and Public Health Department of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of Virology Chadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF) Berlin 10117Germany

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.

关键词： Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human pakistan

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Detection, isolation, and characterization of chikungunya viruses associated with the pakistan outbreak of 2016–2017

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Virologica Sinica 2017年第6期32卷 511-519页

作者： Si-Qinq Liu Xiao Li Ya-Nan Zhang Ai-Li Gao Cheng-Lin Deng Jun-Hua Li Shoukat Jehan Nadia Jamil Fei Deng Hongping Wei Bo Zhang Chinese Academy of Sciences key Laboratory of Special Pathogens and biosafety Center for Emerging Infectious DiseasesWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100012China School of Life Science Northeast Agricultural UniversityHarbin 150036China Centre of Excellence in Science and Applied Technology Islamabad 44000Pakistan State key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China

The chikungunya virus(CHIKV) is a mosquito-transmitted alphavirus, which has infected millions of people in Africa, Asia, Americas, and Europe since it reemerged in India and Indian Ocean regions in 2005–2006. Starting in the middle of November 2016, CHIKV has been widely spread, and more than 4,000 cases of infections in humans were confirmed in pakistan. Here, we report the first isolation and characterization of CHIKV from the pakistan outbreak. Eight CHIKV strains were newly isolated from human serum samples using a cell culture procedure. A full-length genome sequence and eight complete envelope(E1) sequences of CHIKV from pakistan were obtained in this study. Alignment of the CHIKV E1 sequences revealed that the eight new CHIKV isolates were highly homogeneous, with only two nonsynonymous substitutions found at generally conserved sites(E99 and Q235). Based on the comparison of 342 E1 sequences, the two nonsynonymous mutations were located in well-recognized domains associated with viral functions such as the cell fusion and vector specificity, suggesting their potential functional importance. Phylogenetic analysis indicated that the CHIKV strains from pakistan originated from CHIKV circulating in the Indian region. This study helps elucidate the epidemics of CHIKV in pakistan and also provides a foundation for studies of evolution and expansion of CHIKV in South Asia.

关键词： chikungunya virus(CHIKV) pakistan virus isolation clinical serum molecular characterization

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Extensive evolution analysis of the global chikungunya virus strains revealed the origination of CHIKV epidemics in pakistan in 2016

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Virologica Sinica 2017年第6期32卷 520-532页

作者： Junming Shi Zhengyuan Su Zhaojun Fan Jun Wang Siqing Liu Bo Zhang Hongping Wei Shoukat Jehan Nadia Jamil Shu Shen Fei Deng State key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Centre of Excellence in Science and Applied Technology Islamabad 44000Pakistan

Chikungunya virus(CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mosquito species and their distributions. In this study, we reported the complete genome sequences of eight CHIKV isolates from the outbreak in pakistan last year. Then we reviewed the evolutionary history using extensive phylogenetic analysis, analyzed lineagespecific substitutions in viral proteins, and characterized the spreading pathway of CHIKV strains including the pakistani strains. The results showed that the pakistani stains belonged to the *** sub-lineage and derived from India. The genetic properties of the pakistani strains including the adaptive substitution to vectors were further characterized, and the potential risks from the occurrence of CHIKV infection in pakistan were discussed. These results provided better understanding of CHIKV evolution and transmission in the world and revealed the possible origination of the CHIKV outbreak and epidemic in pakistan, which would promote the disease prevention and control in the identified countries and territories with the history of CHIKV infections as well as new regions with potential risk of CHIKV outbreaks.

关键词： chikungunya virus(CHIKV) pakistan phylogenetic analysis

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