Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

作     者：Ali Zohaib Muhammad Saqib Muhammad Ammar Athar Jing Chen Awais-ur-Rahman Sial Saeed Khan Zeeshan Taj Halima Sadia Usman Tahir Muhammad Haleem Tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal Ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail Sajid Victor Max Corman Bing Yan Zheng-Li Shi 

作者机构：CAS Key Laboratory of Special Pathogens and BiosafetyWuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of SciencesBeijing 100049China Faculty of Veterinary MedicineUniversity of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid BiopsyCollege of PharmacyHenan UniversityHenan 475004China Department of Clinical StudiesFaculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health SciencePakistan Karachi 74200 Department of MicrobiologyGovernment College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD)Lahore 54000Pakistan College of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan State Key Laboratory of Agricultural MlcroblologyHuazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health SciencePakistan Jamshoro 76090 Section of Epidemiology and Public HealthDepartment of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of VirologyChadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF)Berlin 10117Germany 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2018年第33卷第5期

页      面：410-417页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 

基　　金：supported by the International Cooperation on Key Technologies of Biosafety along the China-Pakistan Economic Corridor(153B42KYSB20170004) by the External Cooperation Program of CAS(153211KYSB20160001) 

主　　题：Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human Pakistan 

摘      要：Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.