检索结果-南通市图书馆

Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John's wort

引用

Molecular plant 2024年第9期17卷 1439-1457页

作者： Song Wu Ana Luisa Malaco Morotti Jun Yang Ertao Wang Evangelos C.Tatsis national Key Laboratory of plant Molecular Genetics CASs Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChineseAcademy of SciencesShanghai 200032China University of Chinese Academy of Sciences Shanghai 200032China UEPAMS-CAS-JICs Centre of Excellencefor plant anamlcroblalSclence Snangnal 200032Cnina

Hyperforin is the compound responsible for the effectiveness of ***'s wort(Hypericum perforatum)as an antidepressant,but its complete biosynthetic pathway remains *** discovery based on co-expression analysis of bulk RNA-sequencing data or genome mining failed to discover the missing steps in hyperforin *** this study,we sequenced the 1.54-Gb tetraploid *** genome assem-bled into 32 chromosomes with the scaffold N50 value of 42.44 *** single-cell RNA sequencing,we iden-tified a type of cell,“Hyper cells”,wherein hyperforin biosynthesis de novo takes place in both the leaves and *** pathway reconstitution in yeast and tobacco,we identified and characterized four transmembrane prenyltransferases(HpPT1-4)that are localized at the plastid envelope and complete the hyperforin biosynthetic *** hyperforin polycyclic scaffold is created by a reaction cascade involving an irregular isoprenoid coupling and a tandem *** findings reveal how and where hy-perforin is biosynthesized,enabling synthetic-biology reconstitution of the complete ***,this study not only deepens our comprehension of specialized metabolism at the cellularlevel but also provides strategic guidance for elucidation of the biosynthetic pathways of other specializied metabolites in plants.

关键词： hyperforin Hypericum perforatum de novo genome sequencing single-cell RNA sequencing biosyn-thetic pathway meroterpenoids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Development and Standardization of Testing Methods for Genetically Modified Organisms and their Derived Products

引用

Journal of Integrative plant Biology 2011年第7期53卷 539-551页

作者： Dabing Zhang Jinchao Guo national Center for Molecular Characterization of Genetically Modified Organisms School of Life Sciences and Technology Shanghai JiaoTong University Shanghai 200240 China

As the worldwide commercialization of genetically modified organisms （GMOs） increases and consumers concern the safety of GMOs, many countries and regions are issuing labeling regulations on GMOs and their products. Analytical methods and their standardization for GM ingredients in foods and feed are essential for the implementation of labeling regulations. To date, the GMO testing methods are mainly based on the inserted DNA sequences and newly produced proteins in GMOs. This paper presents an overview of GMO testing methods as well as their standardization.

关键词： DNA genetically modified organisms protein standardization testing methods

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Seed metabolomic study reveals significant metabolite variations and correlations among different soybean cultivars

引用

Journal of Integrative plant Biology 2014年第9期56卷 826-836页

作者： Hong Lin Jun Rao Jianxin Shi Chaoyang Hu Fang Cheng Zoe A.Wilson Dabing Zhang Sheng Quan national Center for the Molecular Characterization of Genetically Modified Organisms School of Life Sciences and BiotechnologyShanghai Jiao Tong University Department of plant and Crop Sciences School of BiosciencesUniversity of NottinghamSutton Bonington Campus Shanghai Ruifeng Agricultural Science and Technology Co. Ltd.

Soybean[Glycine max（L.） Merr.]is one of the world＇s major crops,and soybean seeds are a rich and important resource for proteins and *** ＂omics＂ studies,such as genomics,transcriptomics,and proteomics,have been widely applied in soybean molecular research,fewer metabolomic studies have been conducted for largescale detection of low molecular weight metabolites,especially in soybean *** this study,we investigated the seed metabolomes of 29 common soybean cultivars through combined gas chromatography-mass spectrometry and ultra-performance liquid chromatography-tandem mass *** hundred sixty-nine named metabolites were identified and subsequently used to construct a metabolic network of mature soybean *** the 169 detected metabolites,104 were found to be significantly variable in their levels across tested *** markers that could be used to distinguish genetically related soybean cultivars were also identified,and metabolitemetabolite correlation analysis revealed some significant associations within the same or among different metabolite *** from this work may potentially provide the basis for further studies on both soybean seed metabolism and metabolic engineering to improve soybean seed quality and yield.

关键词： Gas chromatography-mass spectrometry(GC-MS) metabolic network metabolite-metabolite correlation metabolomics soybean ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gene diagnosis and targeted breeding for blast-resistant Kongyu 131without changing regional adaptability

引用

Journal of Genetics and Genomics 2018年第10期45卷 539-547页

作者： Xiangchun Zhou Gonghao Jiang Longwei Yang Lei Qiu Ping He Chunxiao Nong Yunyue Wang Yuqing He Yongzhong Xing national Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China College of Life Science Heilongjiang UniversityHarbin 150080China Hubei Collaborative Innovation Center for Grain Industry Jingzhou 434025China Key Laboratory of plant Pathology Yunnan Agricultural UniversityKunming 650224China

The fungus Magnaporthe oryzae threatens the rice production of Kongyu 131 (KY131),a leading japonica variety in Northeast *** this study,two rice lines,KP1 and KP2-Hd1,were obtained by introgressing the blast resistance genes Pi1 and Pi2 into KY131,***,both lines headed later than ***60K SNP array analysis showed that Hd1 closely linked to Pi2 was introgressed into KP2-Hd1,and the linkage drag of Hd1 was broken by *** the other hand,no known flowering genes were introgressed into *** diagnosis by resequencing six flowering genes showed that KP1 carried functional Hd16 and Ghd8 *** to its suppression role in heading under long-day conditions,Ghd8 was chosen as the target for gene editing to disrupt its *** sgRNAs targeting different sites within Ghd8 were utilized to induce large-deletion mutations,which were easy to detect via agarose gel *** the ghd8-mutated KP1 lines were resistant to rice blast disease and headed earlier than the control KP1,even than KY131,under natural long-day conditions,which ensures its growth in Northeast *** study confirmed that a combination of gene diagnosis and targeted gene editing is a highly efficient way to quickly eliminate undesired traits in a breeding line.

关键词： Blast resistance Heading date Gene diagnosis Large deletion Gene editing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Effect of Dietary Supplementation with Phytase transgenic Corn on Growth Performance,Phosphorus Utilization and Excretion in Growing Pigs (Sus scrofa)

引用

Agricultural Sciences in china 2011年第5期10卷 769-776页

作者： WANG Xiu-qi WANG Sheng ZHANG Jun-min YANG Jing-pei Guandong Provincial Key Laboratory ofAgro-Animal Genomics and Molecular Breeding＇/College of Animal Science South China Agricultural University Guangzhou 510642 P.R. China State Key Laboratory of Animal Nutrition Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China

Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn （PTC） on growth performance,phosphorus （P） utilization and excretion in growing *** Exp.1,180 pigs （Large White × Landrace,BW=37.7 kg） were randomly allotted to 4 treatments with 5 replicates of 9 pigs each in order to evaluate the effect of PTC supplementation in low-P diets on growth *** corn soybean meal-based diets consisted of a positive control （PC） diet,a diet containing 500 units （U） of exogenous phytase kg-1 （EP） on the basis of low-P （inorganic P reduced by 0.05% from PC diet） and the low-P＋500 （PTC1） or 750 （PTC2） phytase U of PTC *** Exp.2,20 barrows （Large White×Landrace,BW=31 kg,4 treatments with 5 replicates of 1 pig each） were randomly selected to evaluate the effect of PTC in low-P diets on serum parameters and nutrient *** in Exp.2 were similar to those in Exp.1 except that the EP group was replaced by a low-P diet without exogenous phytase supplementation as a negative control （NC） *** results from Exp.1 showed that the average daily gain （ADG） in the PTC2 group was significantly higher （P〈0.05） than that in the EP group over all *** the other hand,the feed：gain （F：G） ratio of the EP group was significantly higher （P〈0.05） than that of the PTC2 group during 1-21 and 1-42 d,*** were no differences in average daily feed intake （ADFI） among all treatments （P〉0.05）.The results from Exp.2 showed that the concentration of serum Ca in the NC group was the highest （P〈0.05）,while the concentration of serum P in the PTC2 group was the highest （P〈0.05） among all *** was a significant decrease （P〈0.05） in the P apparent digestibility of the NC group compared with the other groups,and that of PTC2 group was the ***,fecal P excretion was reduced （P〈0.05） from 1.80 g d-1 in the PC group to 1.28 g d-1 in the PTC2 *** conclusion,dietary supplementation with PTC

关键词： phytase transgenic corn growth performance nutrient apparent digestibility phosphorus excretion growing pigs

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The regulatory network mediated by circadian clock genes is related to heterosis in rice

引用

Journal of Integrative plant Biology 2015年第3期57卷 300-312页

作者： Guojing Shen Wei Hu Bo Zhang Yongzhong Xing national Key Laboratory of Crop Genetic Improvement and national Center of plant Gene Research (Wuhan) Huazhong Agricultural University

Exploitation of heterosis in rice（Oryza sativa L.） has contributed greatly to global food *** this study,we generated three sets of reciprocal F1 hybrids of indica and japonica subspecies to evaluate the relationship between yield heterosis and the circadian *** were no differences in trait performance or heterosis between the reciprocal hybrids,indicating no maternal effects on *** indica-indica and indica-japonica reciprocal F1 hybrids exhibited pronounced heterosis for chlorophyll and starch content in leaves and for grain yield/*** contrast,the japonica-japonica F1 hybrids showed low *** three circadian clock genes investigated expressed in an above-high-parent pattern（AHP）at seedling stage in all the *** five genes downstream of the circadian clock,and involved in chlorophyll and starch metabolic pathways,were expressed in AHP in hybrids with strong better-parent heterosis（BPH）.Similarly,three of these Research Arfive genes in the japonica-japonica F1 hybrids showing low BPH were expressed in positive overdominance,but the other two genes were expressed in additive or negative *** results indicated that the expression patterns of circadian clock genes and their downstream genes are associated with heterosis,which suggests that the circadian rhythm pathway may be related to heterosis in rice.

关键词： Circadian clock better-parent heterosis biomass overdominance yield

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Short Panicle 3 controls panicle architecture by upregulating APO2/RFL and increasing cytokinin content in rice

引用

Journal of Integrative plant Biology 2019年第9期61卷 987-999页

作者： Yong Huang Xufeng Bai Meifang Luo Yongzhong Xing national Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China

Inflorescence architecture is a major determinant of spikelet numbers per panicle, a key component of grain yield in rice. In this study, Short Panicle 3(SP3) was identified from a short panicle 3(sp3) mutant in which T-DNA was inserted in the promoter of SP3, resulting in a knockdown mutation. SP3 encodes a DNA binding with one finger(Dof) transcriptional activator. Quantitative real time(q RT)-PCR and RNA in situ hybridization assays confirmed that SP3 is preferentially expressed in the young rice inflorescence, specifically in the branch primordial regions. SP3 acts as a negative regulator of inflorescence meristem abortion by upregulating APO2/RFL. SP3 both up-and downregulates expression of genes involved in cytokinin biosynthesis and catabolism, respectively. Consequently, cytokinin concentrations are decreased in young sp3 panicles, thereby leading to small panicles having fewer branches and spikelets. Our findings support a model in which SP3 regulates panicle architecture by modulating cytokinin homeostasis. Potential applications to rice breeding, through gene-editing of the SP3 promoter are assessed.

关键词： DNA controls panicle architecture RFL APO

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Xoryp_08180 of Xanthomonas oryzae ***,Encoding a Hypothetical Protein,is Regulated by HrpG and HrpX and Required for Full Virulence in Rice

引用

Journal of Integrative Agriculture 2012年第4期11卷 600-610页

作者： SHEN Yi-ping ZOU Li-fang LI Yu-rong ZOU Hua-song LIU Xi-ling CHEN Gong-you School of Agriculture and Biology Shanghai Jiao Tong University/Key Laboratory of Urban （South） Ministry of Agriculture Shanghai 200240 P.R. China Department of plant Protection Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests Ministry of Education Nanjing 210095 P.R. China

Xanthomonas oryzae ***（Xoc） causes a destructive bacterial leaf streak disease in *** of the gene products annotated as hypothetical proteins in the genome of Xoc may contribute to its virulence in rice.A mutant,Mxoc1679,screened from our previous Tn5-tagged mutant library for Xoc strain RS105,showed reduced virulence in *** this mutant,a gene named as Xoryp_08180 was disrupted by Tn5 ***_08180 encodes a 1 306-aa hypothetical protein which is highly conserved in Xanthomonas ***-polar mutation of Xoryp_08180 in RS105 strain led to a significant reduction in bacterial virulence and growth in rice,a delayed hypersensitive response（HR） in non-host tobacco,and a decrease in extracellular protease *** deficiencies above were restored to wild-type level in the complementary strain by expressing Xoryp_08180 in *** addition,the expression of Xoryp_08180 was repressed in hrpG and hrpX mutants in planta but not in a nutrient-rich *** results suggested that Xoryp_08180 is a virulence factor required for extracellular protease production,HR induction and full virulence of Xoc.

关键词： Xanthomonas oryzae pv.oryzicola Xoryp_08180 hypothetical protein virulence hypersensitive response extracellular protease

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparative study of the promotion of porcine fetal fibroblast proliferation by overexpression of two transcriptional variants of SIRT6

引用

Chinese Science Bulletin 2013年第10期58卷 1169-1174页

作者： XIE XiaoXian HE Kan ZHANG AiLing ZHANG Hao WANG QiShan LI JiaQi MA YuFang School of Agriculture and Biology Department of Animal SciencesShanghai Jiao Tong UniversityShanghai Key Laboratory of Veterinary Biotechnology Department of Animal Genetics Breeding and ReproductionCollege of Animal SciencesSouth China Agricultural University Guangdong Provincial Key Laboratory of Agroanimal Genomics and Molecular Breeding College of Animal SciencesSouth China Agricultural University

Mammalian Sirtuin 6(SIRT6) plays an important role in the transcriptional silencing of silent mating-type loci,telomeres,and ***,the roles of porcine SIRT6 in cell proliferation are poorly understood,and a better knowledge of these will help improve our understanding of the biological mechanisms of cell growth and *** this study,a novel variant of porcine SIRT6(SIRT6 V2) identified by reverse transcription-polymerase chain reaction and BLAST analysis showed a 124-bp deletion compared to wild-type SIRT6 mRNA(SIRT6 V1).Two recombinant plasmids overexpressing SIRT6 V1 and SIRT6 V2 were produced and their roles in the proliferation of porcine fetal fibroblasts(PFFs) were *** transfected with SIRT6 V1 proliferated significantly faster than those transfected with SIRT6 V2(P<0.01),though both proliferated significantly faster than cells expressing an empty vector(P<0.01).These results might be caused by altered proportions of α helices and β sheets in the SIRT6 V2 structure relative to SIRT6 *** results indicate that overexpression of SIRT6 V1/V2 was involved in promoting PFF *** of a 124-bp sequence attenuated the effects of SIRT6 on cell proliferation,possibly as a result of changes in the proportions of α helices and β sheets in the protein secondary structure.

关键词：成纤维细胞增殖胎儿成纤维细胞 DNA转录过度表达猪变体聚合酶链反应 BLAST

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of plant Density on Yield and Canopy Micro Environment in Hybrid Cotton

引用

Journal of Integrative Agriculture 2014年第10期13卷 2154-2163页

作者： YANG Guo-zheng LUO Xue-jiao NIE Yi-chun ZHANG Xian-long College of plant Science and Technology Huazhong Agricultural University Key Laboratory of Crop Ecophysiology and Farming System in Middle Reaches of the Yangtze River Ministry of Agriculture

A rational plant population is an important attribute to high yield of cotton, because it can provide a beneficial micro environment within the canopy for plant growth and development as well as yield formation. A 2-yr field experiment was conducted to determine the optimal plant density based on cotton yield in relation to the canopy micro environment （canopy temperature, relative humidity and light transmittance）. Six plant densities （1.2-5.7 plants m^-2） were arranged with a completely randomized block design. The highest cotton yield （1 507 kg ha^-1） was obtained at 3.0 plants m^-2 due to more bolls per unit ground area （79 bolls m2）, while the lowest yield （1 091 kg ha1） was obtained at 1.2 plants m^-2. Under the moderate plant density （3.0 plants m^-2）, there was a lower mean daily temperature （MDT, 27. 1℃） attributing to medium daily minimum temperature （Train, 21.9℃） and the lowest daily maximum temperature （Tmax, 35.8℃）, a moderate mean canopy light transmittance of 0.51, and lower mean daily relative humidity （MRH） of 79.7% from June to October. The results suggest that 3.0 plants m^-2 would be the optimal plant density because it provides a better canopy micro environment.

关键词： cotton canopy environment plant density yield light transmittance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：