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Study on Burning Temperature Versus Time Graph of Moxa Sticks in a Novel Animal Moxibustion Device

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Journal of Acupuncture and Tuina Science 2014年第3期12卷 194-198页

作者： Zhou Jun-mei Wei Fang Liu Su-jun Zhang Bi-meng Han Chou-ping Department of Acupuncture and Moxibustion Shanghai First People's Hospital Shanghai Jiaotong University School of Medicine Department of Central Lab Shanghai First People's HospitalShanghai Jiaotong University School of Medicine

Objective: To invent a novel animal experimentation moxa device and intend to solve temperature fluctuations during moxa stick burning in animal moxibustion test. Methods: Smokeless moxa sticks of 4 mm in diameter and 120 mm in length were selected in this trial. The target temperature was(47±1) ℃. The burning temperature versus time graph of moxa sticks during un-interfered time period were recorded when the distances between moxa sticks and thermometer were 2 mm, 3 mm and 4 mm respectively. In addition, the total lengths of burned moxa stick ashes within 0-5 min were recorded. Then the moxa stick burning temperature versus time graph after intervention was measured. Results: The maximum temperatures during test duration when the distances were 2 mm, 3 mm and 4 mm were(47.37±0.25) ℃,(42.62±2.05) ℃ and(35.50±0.40) ℃respectively the total lengths of burned ashes from 0-5 min were(5.1±0.08) mm,(12.17±0.44) mm,(14.8±0.31) mm,(17.25±0.17) mm and(19.82±0.67) mm respectively with a distance of 2 mm between a moxa stick and the testing area, remove 5 mm ashes every 2 min, then the maximum and minimum temperatures within 10 min were(48.37±0.47) ℃and(47.12±0.25) ℃, showing no statistical significance(P=0.12). Conclusion: The novel animal experimentation moxa device can help to maintain relatively constant moxa stick burning temperature during test period.

关键词： Moxibustion Therapy Moxa Stick Moxibustion Animal Experimentation Instrumentation

Identification of global alternative splicing and sex-specific splicing via comparative transcriptome analysis of gonads of Chinese tongue sole(Cynoglossus semilaevis)

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Zoological Research 2022年第3期43卷 319-330页

作者： Yi-Fang Lu Qian Liu Kai-Qiang Liu Hong-Yan Wang Cheng-Hua Li Qian Wang Chang-Wei Shao School of Marine Sciences Ningbo UniversityNingboZhejiang 315211China Key Lab of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoShandong 266071China laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and TechnologyQingdaoShandong 266000China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China

Chinese tongue sole(Cynoglossus semilaevis)is an economically important marine fish species with a ZZ/ZW sex determination mechanism,which can be influenced by *** splicing(AS)is an important mechanism regulating the expression of genes related to sex determination and gonadal differentiation,but has rarely been reported in *** this study,to explore the molecular regulatory mechanisms of sex determination and gonadal differentiation,we combined isoform and RNA sequencing(Iso-Seq and RNA-Seq)to perform transcriptome profiling of male and female gonads in *** total,81883 and 32341 full-length transcripts were obtained in males and females,respectively.A total of 8279 AS genes were identified,including 2639 genes showing differential AS(DAS)between males and *** intersecting DAS genes and differentially expressed genes(DEGs)were enriched in the meiotic cell cycle pathway,and genes related to gonadal differentiation,such as esrrb and wt1a,were found to have sex-specific ***,this study revealed AS events in the gonadal transcriptomes of male and female ***,described the characteristics of active transcription in the testes,and identified candidate genes for studying the regulatory mechanisms of AS during gonadal differentiation.

关键词： Gonadal differentiation Alternative splicing Sex-specific splicing Cynoglossus semilaevis Iso-Seq RNA-Seq

Overexpression of Hdac6 enhances resistance to virus infection in embryonic stem cells and in mice

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Protein & Cell 2015年第2期6卷 152-156页

作者： Dekun Wang Qingwen Meng Lihong Huo Meng Yang Lingling Wang Xinyu Chen JianchaoWang Zhiguo Li Xiaoying Ye Na Liu Qiuyan Li Zhen Dai Hongsheng Ouyang Ning Li Jun Zhou Lingyi Chen Lin Liu State Key laboratory of Medicinal Chemical Biology Collaborative Innovation Center for Bietherapy Department of Genetics and Cell Biology College of Life Sciences Nankai University Tianjin 300071. China State Key laboratory of Veterinary Biotechnology Harbin Veter- inary Research Institute Chinese Academy of Agricultural Sci- ences Harbin 150001 China State Key laboratory for Agrobiotechnology College of Biological Sciences China Agricultural University Beijing 100193 China College of Animal Science and Veterinary Medicine Jilin Univer- sity Changchun 130062 China

Dear Editor Histone deacetylase 6 （Hdac6） is a mostly cytoplasmic class II HDAC. Many proteins have been identified as substrates of Hdac6. Among them, the most well characterized sub- strate of Hdac6 is a-tubulin. Through deacetylating acety- lated lysine 40 in a-tubulin, Hdac6 modulates the acetylation of microtubules （Hubbert et al., 2002）.

关键词： Embryonic Stem Embryonic Stem Cell H5N1 Virus Oral Squamous Cell Carcinoma Avian H5N1 Influenza