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An Automatic Implementation of Oropharyngeal Swab Sampling for Diagnosing Respiratory Infectious Diseases via Soft Robotic End-Effectors

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Chinese Journal of Mechanical Engineering 2024年第2期37卷 55-67页

作者： Yafeng Cui Wenjie Yu Jingjing Li Qi Shao Ding Weng Guoping Yin Xiaohao Zhang Xinjun Liu Jingying Ye Jiadao Wang Huichan Zhao Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China Beijing Huakang Tongbang Technology Company Beijing 102609China Department of Otorhinolaryngology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China Tianjin research Institute for Advanced Equipment Tsinghua UniversityTianjin 300300China

The most widely adopted method for diagnosing respiratory infectious diseases is to conduct polymerase chain reaction(PCR)assays on patients’respiratory specimens,which are collected through either nasal or oropharyngeal *** manual swab sampling process poses a high risk to the examiner and may cause false-negative results owing to improper *** this paper,we propose a pneumatically actuated soft end-effector specifically designed to achieve all of the tasks involved in swab *** soft end-effector utilizes circumferential instability to ensure grasping stability,and exhibits several key properties,including high load-to-weight ratio,error tolerance,and variable swab-tip stiffness,leading to successful automatic robotic oropharyngeal swab sampling,from loosening and tightening the transport medium tube cap,holding the swab,and conducting sampling,to snapping off the swab tail and sterilizing *** an industrial collaborative robotic arm,we integrated the soft end-effector,force sensor,camera,lights,and remote-control stick,and developed a robotic oropharyngeal swab sampling *** this swab sampling system,we conducted oropharyngeal swab-sampling tests on 20 *** Digital PCR assay results(RNase P RNA gene absolute copy numbers for the samples)revealed that our system successfully collected sufficient numbers of cells from the pharyngeal wall for respiratory disease *** summary,we have developed a pharyngeal swab-sampling system based on an“enveloping”soft actuator,studied the sampling process,and imple-mented whole-process robotic oropharyngeal swab-sampling.

关键词： Diagnosis Medical robot Soft end-effector Swab-sampling Digital PCR

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A duplex TaqMan probe-based real-time PCR method for rapid detection and differentiation of the classical and VB strains of human immunodeficiency virus

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Science China(Life Sciences) 2024年第1期67卷 208-210页

作者： Aiping Wang Haili Wang Jianguo Zhao Yumei Chen Hongliang Liu Peiyang Ding Yan Niu Gaiping Zhang Longhu Laboratory Zhengzhou 450046China School of Advanced Agricultural Sciences Peking UniversityBeijing 100871China

Dear Editor,Human immunodeficiency virus(HIV)is a lentivirus that,if left untreated,can lead to acquired immunodeficiency syndrome(AIDS).The virus can be divided into two types based on genetic differences:HIV-1 and ***-1 is widely distributed worldwide and is the primary strain responsible for the global AIDS epidemic due to its high replication capacity,greater likelihood of transmission,and more severe clinical symptoms(Oeschger et al.,2021).

关键词： immunodeficiency untreated

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Effects of traditional Chinese medicine on treatment outcomes in severe covid-19 patients:a single-centre study

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Chinese Journal of Natural Medicines 2024年第1期22卷 89-96页

作者： XIAO Yongjiu LI Binbin LIU Chang HUANG Xiuyu MA Ling QIAN Zhirong ZHANG Xiaopeng ZHANG Qian LI Dunqing CAI Xiaoqing YAN Xiangyong LUO Shuping XIANG Dawei XIAO Kun emergency Department the 940th Hospital of Jiont Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Scientific research Center the Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518000China School of Medicine Nankai universityTianjin 300000China Department of Cardiology the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Department of Radiation Oncology Fujian Medical University Union HospitalFuzhou 350000China Department of Traditional Chinese Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Department of Geriatric Diseases the Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China Health and Sport Administration Muskingum UniversityOhioanUSA Laboratory of Vaccine and Antibody Engineering Beijing Institute of BiotechnologyBeijing 100071China Department of Pulmonary and Critical Care Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyLanzhou 730050China Center of Pulmonary&Critical Care Medicine Chinese People’s Liberation Army(PLA)General HospitalBeijing 100853China

As the search for effective treatments for covid-19 continues,the high mortality rate among critically ill patients in Intensive Care Units(ICU)presents a profound *** study explores the potential benefits of traditional Chinese medicine(TCM)as a supplementary treatment for severe covid-19.A total of 110 critically ill covid-19 patients at the Intensive Care Unit(ICU)of Vulcan Hill Hospital between Feb.,2020,and April,2020(wuhan,China)participated in this observational *** patients received standard supportive care protocols,with a subset of 81 also receiving TCM as an adjunct *** characteristics during the treatment period and the clinical outcome of each patient were closely monitored and *** findings indicated that the TCM group exhibited a significantly lower mortality rate compared with the non-TCM group(16 of 81 vs 24 of 29;0.3 vs 2.3 person/month).In the adjusted Cox proportional hazards models,TCM treatment was associated with improved survival odds(Pcovid-19 patients.

关键词： covid-19 Pneumonia Novel coronavirus Critically ill Traditional Chinese medicine Clinical outcome

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Association between glucose levels of children with type 1 diabetes and parental economic status in mobile health application

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World Journal of Diabetes 2024年第7期15卷 1477-1488页

作者： Wen-Hao Zhang Chao-Fan Wang Hao Wang Jie Tang Hong-Qiang Zhang Jiang-Yu Zhu Xue-Ying Zheng Si-Hui Luo Yu Ding Department of Endocrinology The First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina Department of Endocrinology and Metabolism The Third Affiliated Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of DiabetologyGuangzhou 510000Guangdong ProvinceChina Graduate School Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina

BACKGROUND The glycemic control of children with type 1 diabetes(T1D)may be influenced by the economic status of their *** To investigate the association between parental economic status and blood glucose levels of children with T1D using a mobile health *** Data from children with T1D in China's largest T1D online community,Tang-TangQuan■.Blood glucose levels were uploaded every three months and parental economic status was evaluated based on annual household *** were divided into three groups:Low-income(100000 yuan)(1 Yuan=0.145 United States Dollar approximately).Blood glucose levels were compared among the groups and associations were explored using Spearman’s correlation analysis and multivariable logistic *** From September 2015 to August 2022,1406 eligible children with T1D were included(779 female,55.4%).Median age was 8.1 years(Q1-Q3:4.6-11.6)and duration of T1D was 0.06 years(0.02-0.44).Participants were divided into three groups:Low-income(n=320),middle-income(n=724),and high-income(n=362).Baseline hemoglobin A1c(HbA1c)levels were comparable among the three groups(P=0.072).However,at month 36,the low-income group had the highest HbA1c levels(P=0.036).Within three years after registration,glucose levels increased significantly in the low-income group but not in the middle-income and high-income *** economic status was negatively correlated with pre-dinner glucose(r=-0.272,P=0.012).After adjustment for confounders,parental economic status remained a significant factor related to pre-dinner glucose levels(odds ratio=13.02,95%CI:1.99 to 126.05,P=0.002).CONCLUSION The blood glucose levels of children with T1D were negatively associated with parental economic *** is suggested that parental economic status should be taken into consideration in the management of T1D for children.

关键词： Type 1 diabetes Children and adolescents Glycemic control Economic status Mobile health application

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Effects of traditional Chinese medicine on treatment outcomes in severe covid-19 patients: a single-centre study

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Chinese Journal of Natural Medicines 2024年第01期 89-96页

作者： XIAO Yongjiu LI Binbin LIU Chang HUANG Xiuyu MA Ling QIAN Zhirong ZHANG Xiaopeng ZHANG Qian LI Dunqing CAI Xiaoqing YAN Xiangyong LUO Shuping XIANG Dawei XIAO Kun emergency Department the 940th Hospital of Jiont Logistics Support Force of Chinese People's Liberation Army Scientific research Center the Seventh Affiliated Hospital Sun Yat-sen University School of Medicine Nankai university Center of Pulmonary & Critical Care Medicine Chinese People's Liberation Army (PLA) General Hospital Department of Cardiology the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army Department of Radiation Oncology Fujian Medical University Union Hospital Department of Traditional Chinese Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army Department of Geriatric Diseases the Seventh Medical Center of Chinese PLA General Hospital Health and Sport Administration Muskingum University Laboratory of Vaccine and Antibody Engineering Beijing Institute of Biotechnology Department of Pulmonary and Critical Care Medicine the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army

As the search for effective treatments for covid-19 continues, the high mortality rate among critically ill patients in Intensive Care Units(ICU) presents a profound challenge. This study explores the potential benefits of traditional Chinese medicine(TCM) as a supplementary treatment for severe covid-19. A total of 110 critically ill covid-19 patients at the Intensive Care Unit(ICU) of Vulcan Hill Hospital between Feb., 2020, and April, 2020(wuhan, China) participated in this observational study. All patients received standard supportive care protocols, with a subset of 81 also receiving TCM as an adjunct treatment. Clinical characteristics during the treatment period and the clinical outcome of each patient were closely monitored and analysed. Our findings indicated that the TCM group exhibited a significantly lower mortality rate compared with the non-TCM group(16 of 81 vs 24 of 29; 0.3 vs 2.3person/month). In the adjusted Cox proportional hazards models, TCM treatment was associated with improved survival odds(P covid-19 patients.

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Collective Movement Simulation:Methods and Applications

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Machine Intelligence research 2024年第3期21卷 452-480页

作者： Hua Wang Xing-Yu Guo Hao Tao Ming-Liang Xu School of Computer and Artificial Intelligence Zhengzhou UniversityZhengzhou450001China Engineering research Center of Intelligent Swarm Systems Ministry of EducationZhengzhou450001China National Supercomputing Center in Zhengzhou Zhengzhou450001China China Ship Development and Design Center Wuhan430060China

Collective movement simulations are challenging and important in many areas,including life science,mathematics,physics,information science and public *** this survey,we provide a comprehensive review of the state-of-the-art techniques for collective movement *** start with a discussion on certain concepts to help beginners understand it more ***,we analyze the intelligence among different collective objects and the emphasis in different ***,we classify existing collective movement simulation methods into four categories according to their effects,namely versatility,accuracy,dynamic adaptability,and assessment feedback ***,we introduce five applications of layout optimization,emergency control,dispatching,unmanned systems,and other derivative ***,we summarize possible future research directions.

关键词： Collective movement simulation multiple objects multiple discipline simulation effect collective intelligence

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Serologic Response to SARS-CoV-2 in covid-19 Patients with Different Severity

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Virologica Sinica 2020年第6期35卷 752-757页

作者： Wen-Hua Kong Rong Zhao Jun-Bo Zhou Fang Wang De-Guang Kong Jian-Bin Sun Qiong-Fang Ruan Man-Qing Liu wuhan Center for Disease Control and Prevention Wuhan 430024China wuhan Third Hospital Tongren Hospital of Wuhan UniversityWuhan 430060China

The immense patient number caused by coronavirus disease 2019(covid-19)global pandemic brings the urge for more knowledge about its immunological features,including the profile of basic immune *** this study,eighty-eight reported covid-19 patients in wuhan were recruited from January to February,2020,including 32 severe/critical cases and56 mild/moderate *** mean age was 56.43 years(range 17–83)and gender ratio(male/female)was 43:*** tested SARS-CoV-2 RNA with commercial kits,investigated the level of serologic IgM and IgG antibodies against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)using magnetic particle chemiluminescence immunoassays,and compared the results of serologic tests and nucleic acid test(NAT).Among 88 patients,95.45%were confirmed as positive by the combination of NAT and antibody test,which was significantly higher(Pcovid-19 diagnosis and provided evidence on the temporal profile of antibody response in patients with different disease severity.

关键词： covid-19 Nucleic acid test(NAT) Serologic test Real-time polymerase chain reaction(RT-PCT) Chemiluminescence immunoassay(CLIA)

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Short-term developmental outcomes in neonates born to mothers with covid-19 from wuhan, China

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World Journal of Pediatrics 2021年第3期17卷 253-262页

作者： Ling-Kong Zeng Hua-Ping Zhu Tian-Tian Xiao Si-Cong Peng Wen-Hao Yuan Jian-Bo Shao Lai-Shuan Wang Fei-Fan Xiao Shi-Wen Xia Shoo K.Lee Kai Yan Wen-Hao Zhou Department of Neonatology Wuhan Children's Hospital(Wuhan Maternal and Child Healthcare Hospital)Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Neonatology Maternal and Child Health Hospital of Hubei ProvinceWuhanChina National Children’s Medical Center Children’s Hospital of Fudan UniversityShanghaiChina Department of Neonatology Chengdu Women's and Children's Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduChina Department of Neonatology Children's Hospital of Fudan UniversityNational Children's Medical CenterShanghaiChina Maternal-Infant Care research Center(MiCARE)and Department of Pediatrics Mount Sinai HospitalTorontoONCanada Department of Pediatrics Department of Obstetrics and Gynecology and Dal la Lana School of Public HealthUniversity of TorontoTorontoONCanada

Background Coronavirus disease 2019(covid-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is an emerging *** consequences of SARS-CoV-2 exposure in infants remain ***,this study aims to investigate whether neonates born to mothers with covid-19 have adverse brain *** This multicenter observational study was conducted at two designated maternal and children's hospitals in Hubei Province,China's Mainland from February 1,2020 to May 15,*** born to mothers with covid-19 were *** magnetic resonance imaging(MRI)findings,and volumes of grey and white matters,and physical growth parameters were observed at 44 weeks corrected gestational *** Of 72 neonates born to mothers with covid-19,8(11%)were diagnosed with covid-19,8(11%)were critically ill,and no deaths were *** the eight neonates that underwent brain MRI at corrected gestational age of 44 weeks,five neonates were diagnosed with *** these five neonates,three presented abnormal MRI findings including abnormal signal in white matter and delayed myelination in newborn 2,delayed myelination and brain dysplasia in newborn 3,and abnormal signal in the bilateral periventricular in newborn *** other three neonates without covid-19 presented no significantly changes of brain MRI findings and the volumes of grey matter and white matter compared to those of healthy newborns at the equivalent age(P>0.05).Physical growth parameters for weight,length,and head circumference at gestational age of 44 weeks were all above the 3rd percentile for all *** Some of the neonates born to mothers with covid-19 had abnormal brain MRI findings but these neonates did not appear to have poor physical *** findings may provide the information on the follow-up schedule on the neonates exposed to SARS-CoV-2,but further study is required to evaluate the association between the abnormal MRI findings and the exposure

关键词： Coronavirus disease 2019 Follow-up Neonate Severe acute respiratory syndrome coronavirus 2

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Immunogenicity and safety of a severe acute respiratory syndrome coronavirus 2 inactivated vaccine in healthy adults: randomized, double-blind, and placebo-controlled phase 1 and phase 2 clinical trials

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Chinese Medical Journal 2021年第11期134卷 1289-1298页

作者： Hong-Xing Pan Jian-Kai Liu Bao-Ying Huang Gui-Fan Li Xian-Yun Chang Ya-Fei Liu Wen-Ling Wang Kai Chu Jia-Lei Hu Jing-Xin Li Dan-Dan Zhu Jing-Liang Wu Xiao-Yu Xu Li Zhang Meng Wang Wen-Jie Tan Wei-Jin Huang Feng-Cai Zhu NHC Key Laboratory of Enteric Pathogenic Microbiology(Jiangsu Provincial Center for Disease Control and Prevention) NanjingJiangsu 210009China Shenzhen Kangtai Biological Products Co. Ltd.ShenzhenGuangdong 518057China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Beijing Minhai Biological Technology Co. Ltd.Beijing 102600China Huaiyin District Center for Disease Control and Prevention Huai’anJiangsu 223003China Vazyme Biotech Co. LtdNanjingJiangsu 210000China National Institutes for Food and Drug Control Beijing 102629China Center for Global Health Nanjing Medical UniversityNanjingJiangsu 211166China

Background:The significant morbidity and mortality resulted from the infection of a severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)call for urgent development of effective and safe *** report the immunogenicity and safety of an inactivated SARS-CoV-2 vaccine,KCONVAC,in healthy ***:Phase 1 and phase 2 randomized,double-blind,and placebo-controlled trials of KCONVAC were conducted in healthy Chinese adults aged 18 to 59 *** participants in the phase 1 trial were randomized to receive two doses,one each on Days 0 and 14,of either KCONVAC(5 or 10 mg/dose)or *** participants in the phase 2 trial were randomized to receive either KCONVAC(at 5 or 10 mg/dose)or placebo on Days 0 and 14(0/14 regimen)or Days 0 and 28(0/28 regimen).In the phase 1 trial,the primary safety endpoint was the proportion of participants experiencing adverse reactions/events within 28 days following the administration of each *** the phase 2 trial,the primary immunogenicity endpoints were neutralization antibody seroconversion and titer and anti-receptor-binding domain immunoglobulin G seroconversion at 28 days after the second ***:Inthe phase1 trial,60 participantswere enrolled andreceived at least one dose of 5-mgvaccine(n=24),10-mgvaccine(n=24),or placebo(n=12).In the phase 2 trial,500 participantswere enrolled and received at least one dose of 5-mg vaccine(n=100 for 0/14 or 0/28 regimens),10-mg vaccine(n=100 for each regimen),or placebo(n=50 for each regimen).In the phase 1 trial,13(54%),11(46%),and seven(7/12)participants reported at least one adverse event(AE)after receiving 5-,10-mg vaccine,or placebo,*** the phase 2 trial,16(16%),19(19%),and nine(18%)0/14-regimen participants reported at least oneAEafter receiving 5-,10-mg vaccine,or placebo,*** AE incidences were observed in the three 0/28-regimen treatment *** AEs with an intensity of grade 3+were reported,expect for one vaccine-unrelated seriousAE(

关键词： Immunogenicity Safety SARS-CoV-2 Inactivated vaccine Neutralizing antibody

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Clinical features and risk factors associated with severe covid-19 patients in China

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Chinese Medical Journal 2021年第8期134卷 944-953页

作者： Ning Jiang Yan-Nan Liu Jing Bao Ran Li Wen-Tao Ni Xing-Yu Tan Yu Xu Li-Ping Peng Xiao-Rong Wang Yi-Ming Zeng Dai-Shun Liu Qing Xue Jia-Shu Li Ke Hu Ya-Li Zheng Zhan-Cheng Gao Department of Respiratory and Critical Care Medicine Peking University People's HospitalBeijing 100044China Department of Respiratory and Critical Care Medicine The First Hospital of Jilin UniversityChangchunJilin 130021China Department of Respiratory and Critical Care Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Respiratory and Critical Care Medicine The Second Affiliated Hospital of Fujian Medical UniversityQuanzhouFujian 362000China Department of Respiratory and Critical Care Medicine The First People's Hospital of ZunyiZunyiGuizhou 563000China Department of Respiratory and Critical Care Medicine Ningde People's HospitalNingdeFujian 352000China Department of Respiratory and Critical Care Medicine The First People's Hospital of LianyungangLianyungangJiangsu 222061China Department of Respiratory and Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhanHubei 430060China Department of Respiratory Critical Careand Sleep MedicineXiang'an Hospital of Xiamen UniversityXiamenFujian 361111China

Background::Coronavirus disease 2019(covid-19),which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),has rapidly spread throughout the *** this study,we aimed to identify the risk factors for severe covid-19 to improve treatment ***::A multicenter,cross-sectional study was conducted on 313 patients hospitalized with *** were classified into two groups based on disease severity(nonsevere and severe)according to initial clinical *** test results and epidemiological and clinical characteristics were analyzed using descriptive *** and multivariate logistic regression models were used to detect potential risk factors associated with severe ***::A total of 289 patients(197 nonsevere and 92 severe cases)with a median age of 45.0(33.0,61.0)years were included in this study,and 53.3%(154/289)were ***(192/286,67.1%)and cough(170/289,58.8%)were commonly observed,followed by sore throat(49/289,17.0%).Multivariate logistic regression analysis suggested that patients who were aged≥65 years(OR:2.725,95%confidence interval[CI]:1.317-5.636;P=0.007),were male(OR:1.878,95%CI:1.002-3.520,P=0.049),had comorbid diabetes(OR:3.314,95%CI:1.126-9.758,P=0.030),cough(OR:3.427,95%CI:1.752-6.706,Pcovid-19(71.4%vs.28.6%,χ2=8.183,P=0.004).Conclusions::The clinical characteristics of those with severe and nonsevere covid-19 were significantly *** elderly,male patients with covid-19,diabetes,and presenting with cough and/or diarrhea on admission may require close monitoring to prevent deterioration.

关键词： Clinical feature Coronavirus disease 2019 Diabetes Risk factor Severe acute respiratory syndrome coronavirus 2

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