检索结果-南通市图书馆

Efficient production of 14α‑OH‑AD by engineered Mycolicibacterium neoaurum via coupled cofactor and reconstructed electron transport system

引用

Systems Microbiology and Biomanufacturing 2023年第2期3卷 358-369页

作者： Chenwei Zhang Yanbing Shen Yuanyuan Gao Zehui Zan Min Wang Key Laboratory of Industrial Fermentation Microbiology Ministry of EducationTianjin Key Laboratory of Industrial MicrobiologyTianjin University of Science and TechnologyTianjinChina Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of BiotechnologyTianjin University of Science and TechnologyTianjin 300457People’s Republic of China

14α-hydroxy-androst-4-ene-3,17-dione(14α-OH-AD)is an important precursor for the synthesis of steroid drugs with anticancer and carcinolytic ***,14α-OH-AD was mostly synthesized by whole-cell fermentation of mold fungi using androstenedione(AD)as a substrate,which had difficulties in product isolation and purification as well as problems of high production *** this study,the source of the 14α-hydroxylase gene was *** 14α-hydroxylase genes were heterologously expressed in Mycolicibacterium neoaurum(MNR)M3ΔksdD,which enabled the one-step biotransformation from the cheap substrate phytosterols(PS)to 14α-OH-AD,reducing the difficulty of product purification and production *** is more,to alleviate the problem of poor activity of 14α-hydroxylase,the 14α-hydroxylase gene was co-expressed with the electron transport chain element genes and the coenzyme regeneration genes,and a superior engineered strain MNR M3ΔksdD/pMV261-14α-G6PDH was ***,the transformation conditions were optimized for the transformation of PS by the engineered *** molar yield of 14α-OH-AD reached to 60.4±2.3%(about 0.22 g/L productivity).This study investigated for the first time the effects of the tandem electron transport chain element genes and the tandem coenzyme regeneration genes on the 14α-hydroxylation reaction,providing a theoretical basis for the industrial production of 14α-OH-AD.

关键词： 14α-hydroxy-androst-4-ene-3,17-dione Mycolicibacterium neoaurum 14α-hydroxylase Electron transportation Coenzyme regeneration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The environmental adaptability and reproductive properties of invasive green alga Codium fragile from the Nan'ao Island,South China Sea

引用

Acta Oceanologica Sinica 2022年第3期41卷 70-75页

作者： Lanping Ding Xulei Wang Bingxin Huang Weizhou Chen Shanwen Chen College of Life sciences Tianjin Normal UniversityTianjin 300387China Marine Biology Institute Shantou UniversityShantou 515063China tianjin Key Laboratory of Animal and Plant Resistance Tianjin 300387China

It has been widely recognized that biological invasion has become one of the greatest threats to the *** fragile is an invasive species which exhibits a variety of attributes like parthenogenesis,winter fragment,and vegetative reproduction;and therefore,it has become a successful invader,colonizing most subtropical *** China’s southeast coastal aquaculture waters,the green algal bloom caused by *** will probably become a serious *** order to understand more details about the species,an experiment focused on its reproductive characteristics was conducted using culture established from a sample collected in the aquaculture raft of the Nan’ao Island in the South China *** results showed that there were two types of gametes resembling aplanospores and zoospores respectively,both of which were able to *** the gametes liberation,a long mucilage tube was formed out of the mouth of the gametangium assisting dispersal of gametes away from the parent *** tube was adapted not only to its surrounding flowing water environment but also to its parent plant’s outer gelatinous *** general,the optimum temperature for gametes release and germination was 15-20℃ and 15℃,respectively,which corresponded to the local offshore marine *** plant was observed to produce vegetative buds under favourable reproductive conditions which were called *** were capable of developing into filamentous *** results will provide some scientific evidences for revealing the biological mechanism of bloom and control strategies of invasive green algae.

关键词： Codium fragile gametes propagules mucilage tube temperature environmental adaptation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The advances of traditional Chinese medicine in the treatment of liver diseases in 2019

引用

Traditional Medicine Research 2020年第4期5卷 261-271页

作者： Min Cao Jing Miao Li Wang Hai-Zhao Liu Huan-Tian Cui Yu-Hong Bian Graduate School Tianjin University of Traditional Chinese MedicineTianjin 301617China Department of Integrated Traditional and Western Medicine Tianjin Second People’s HospitalTianjin 300192China Department of Pharmacy Tianjin Second People’s HospitalTianjin 300192China Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology School of Life SciencesShandong UniversityQingdao 250100China College of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China

Background:Currently,the treatment of liver diseases remains an unsolved problem due to its complicated etiology and *** Chinese medicine(TCM)has been used for liver disease treatment for thousands of *** treatment using TCM compounds conforms to the concept of“holism”,which coincides with the complicated pathogenic mechanisms of liver ***,the mechanisms have not been clearly explained due to the complex components and multi-targets,which is a big obstacle TCM’s popularity and *** recent years,studying the mechanisms and identifying the novel ingredients in herbal medicines are becoming a hot spot for many ***,we obtained literature in PubMed and summarized the progress of TCM’s active ingredients and formulas in treating various liver diseases in *** on the literature,flavonoids,polysaccharides,saponins,and alkaloids,as well as Chinese medicine formulas,such as Ba-Bao pill and Yin-Chen-Hao decoction,have attracted much *** addition,we also focused on the application of new omics analysis techniques,such as metabolomics,network pharmacology,and other omics analyses in the study of TCM formulas.

关键词： Traditional Chinese medicine Liver diseases Mechanism Metabolomics Network pharmacology Omics analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

引用

Cancer Communications 2023年第12期43卷 1326-1353页

作者： Xiaoteng Cui Jixing Zhao Guanzhang Li Chao Yang Shixue Yang Qi Zhan Junhu Zhou Yunfei Wang Menglin Xiao Biao Hong Kaikai Yi Fei Tong Yanli Tan Hu Wang Qixue Wang Tao Jiang Chuan Fang Chunsheng Kang Laboratory of Neuro-oncology Tianjin Neurological Institute Tianjin Medical University General Hospital Tianjin P. R. China Key Laboratory of Post-Neuro Injury Neuro-repair and Regeneration in Central Nervous System Ministry of Education and Tianjin City Tianjin P. R. China Department of Neurosurgery Beijing Tiantan Hospital Capital Medical University Beijing P. R. China Chinese Glioma Genome Atlas Network (CGGA) and Asian Glioma Genome Atlas Network (AGGA) Beijing P. R. China Department of Neurosurgery The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan P. R. China tianjin Key Laboratory of Composite and Functional Materials School of Material Science and Engineering Tianjin University Tianjin P. R. China Department of Neurosurgery Affiliated Hospital of Hebei University Baoding Hebei P. R. China Hebei Key Laboratory of Precise Diagnosis and Treatment of Glioma Baoding Hebei P. R. China Department of Neuro-Oncology and Neurosurgery Tianjin Medical University Cancer Institute and Hospital Tianjin P. R. China Department of Pathology Affiliated Hospital of Hebei University Baoding Hebei P. R. China Department of Pathology Hebei University School of Basic Medical Sciences Baoding Hebei P. R. China Department of Neurosurgery Tianjin Huanhu Hospital Tianjin P. R. China Department of Molecular Neuropathology Beijing Neurosurgical Institute Capital Medical University Beijing P. R. China Research Unit of Accurate Diagnosis Treatment and Translational Medicine of Brain Tumors Chinese Academy of Medical Sciences Beijing P. R. China

Background Metabolism reprogramming plays a vital role in glioblastoma(GBM)progression and recurrence by producing enough energy for highly proliferating tumor *** addition,metabolic reprogramming is crucial for tumor growth and immune-escape *** growth factor receptor(EGFR)amplification and EGFR-vIII mutation are often detected in GBM cells,contributing to the malignant *** study aimed to investigate the functional role of the EGFR pathway on fatty acid metabolism remodeling and energy *** Clinical GBM specimens were selected for single-cell RNA sequencing and untargeted metabolomics analysis.A metabolism-associated RTK-fatty acid-gene signature was constructed and ***-2206 and MK-803 were utilized to block the RTK pathway and mevalonate pathway induced abnormal *** metabolism in GBM with activated EGFR pathway was *** antitumor effect of Osimertinib and Atorvastatin assisted by temozolomide(TMZ)was analyzed by an intracranial tumor model in *** GBM with high EGFR expression had characteristics of lipid remodeling and maintaining high cholesterol levels,supported by the single-cell RNA sequencing and metabolomics of clinical GBM *** of the EGFR/AKT and mevalonate pathways could remodel energy metabolism by repressing the tricarboxylic acid cycle and modulating ATP ***,the EGFR/AKT pathway upregulated the expressions of acyl-CoA synthetase short-chain family member 3(ACSS3),acyl-CoA synthetase long-chain family member 3(ACSL3),and long-chain fatty acid elongation-related gene ELOVL fatty acid elongase 2(ELOVL2)in an NF-κB-dependent ***,inhibition of the mevalonate pathway reduced the EGFR level on the cell membranes,thereby affecting the signal transduction of the EGFR/AKT ***,targeting the EGFR/AKT and mevalonate pathways enhanced the antitumor effect of TMZ in GBM cells and animal *** Our findings no

关键词： combinatorial therapeutic strategy EGFR energy metabolism glioblastoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Temperature-insensitive sensor for glucose brix measurement based on compact spindle-shaped structure with two-mode fiber

引用

Optoelectronics Letters 2022年第12期18卷 737-742页

作者： DONG Miaoyun TONG Zhengrong ZHANG Weihua LI Peng WU Meng TIAN Xue Engineering Research Center of Optoelectronic Devices and Communication technology Ministry of EducationTianjin Key Laboratory of Film Electronic and Communication DevicesSchool of Integrated Circuit Science and EngineeringTianjin University of TechnologyTianjin300384China

A temperature-insensitive sensor for glucose brix measurement based on compact spindle-shaped structure with two-mode fiber(TMF) is proposed. Due to the bending of optical fiber caused by flame baking, some of the light energy transmitted in the core leaks into the cladding area as an evanescent wave, which excites the higher-order cladding mode of the sensor. The experimental results show that when the length of TMF is 3 cm and the bending diameter is 4 mm, the maximum glucose brix sensitivity of the sensor is 0.368 nm/% from 0 to 21%. The sensor is insensitive to temperature from 10 °C to 50 °C, which can avoid the problem of temperature cross-sensitivity. A compact spindle-shaped sensor is a potential effective sensor with a simple structure, easy fabrication and low cost. The sensor can be used to detect glucose content in areas such as crops quality assessment and the research of pharmacy and bioengineering.

关键词： shaped fiber mode

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Study on heavy metal concentrations in river sediments through the total amount evaluation method

引用

Journal of Zhejiang University-science A(Applied Physics & Engineering) 2011年第5期12卷 399-404页

作者： Chen-chen WANG Zhi-guang NIU Yan LI Jie SUN Fang WANG School of Environment science and technology Tianjin University Tianjin 300072 China Yangtze River Waterway Bureau Encamped in the Binhai Office Tianjin 300072 China tianjin Urban Construction Design Institute Co. Ltd Tianjin 300072 China

A quantitative method to evaluate the amounts of heavy metals in river sediments is established. Using the BT Drainage River in North China as a study object, six representative cross sections were selected for measurement of heavy metal indicators in sediments, and then the main contamination indicators were determined by performing a potential ecological risk assessment. Using a section of this river as an example, the total amounts of the main pollution indicators and those of their harmful forms are estimated by the Surfer software, which simulates the pollution status within the downstream sediments of the outfall at this section. The calculation results could provide a theoretical guideline and data support for pollution treatment of the BT Drainage River.

关键词： Total amount evaluation Heavy metal pollution River sediments Surfer software

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

ADAPTABLE DESIGN OF MACHINE TOOLS STRUCTURES

引用

Chinese Journal of Mechanical Engineering 2008年第3期21卷 7-15页

作者： XU Yanshen CHEN Yongliang ZHANG Guojun GU Peihua School of Mechanical Engineering Tianjin University Tianjin 300072 China School of Mechanical science and Engineering Huazhong University of Science and Technology Wuhan 430074 China Department of Mechatronic Engineering Shantou University Shantou 515063 China School of Mechanical science and Engineering Huazhong University of Science and Technology Wuhan 430074 China Department of Mechanical and Manufacturing Engineering University of Calgary Calgary T2N 1N4 Canada

Adaptable design aims to extend the utilities of design and product. The specific methods are developed for practical applications of adaptable design in the design of mechanical structures, including adaptable platform, interface and module designs. Adaptable redesign problems are formulated as adaptable platform design under adaptability bound constraints. Analysis tools are then suggested for the implementation of the redesign of machine tool structures, including variation techniques based sensitivity analysis, similarity-based commonality analysis, performance improvement, and adaptability measures. The specific measure of adaptability for machine tool structures is measured through the quantification of the structural similarity and performance improvement gained from adaptable design. The method provides designers with an approach that brings adaptability into the design process, with considering the cost and benefits of such adaptability. The redesign of CNC spiral bevel gear cutting machine structures has been included to illustrate these concepts and methods.

关键词： Adaptable design Machine tools

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Roles of metal element substitutions from the bimetallic solid state electrolytes in lithium batteries

引用

Particuology 2022年第6期20卷 51-71页

作者： Wen Yu Nanping Deng Lin Tang Kewei Cheng Bowen Cheng Weimin Kang State Key Laboratory of Separation Membranes and Membrane Processes National Center for International Joint Research on Separation MembranesTiangong UniversityTianjin 300387China School of Textile science and Engineering Tiangong UniversityTianjin 300387China

All-solid-state lithium batteries(ASSLBs),receiving extensive attentions and studies,exhibit better safety,environmental friendliness,stability,wider electrochemical stability window and higher energy density than traditionally liquid lithium *** a variety of inorganic materials,with highly replaceable,the non-lithium metal elements emerge in endlessly and affect performances in diversiform *** to facile preparation,convertible structures and excellent properties,the lithium-containing bimetallic granular materials are often applied as important components of electrolytes in lithium *** this review,in terms of the properties of substituted elements,changing crystal structures,increasing vacancies or defects and improving the interfacial conductions,the roles of metal element substitutions of inorganic particles on the improvement of solid-state electrolytes are *** the applications of substituted strategies in ASSLBs as the host of inorganic particles electrolytes and as fillers or modifications for composite electrolytes are also investigated and *** also summarizes the current concerns and obstacles that need to be broken through,as well as provides a basis guide for the selection and optimization of inorganic particles.

关键词： Metal element substitutions Bimetallic inorganic particles Solid-state electrolytes All-solid-state lithium batteries Mechanisms

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Complement C3a activates osteoclasts by regulating the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma

引用

Cancer Biology & Medicine 2021年第3期18卷 721-733页

作者： Fengjuan Jiang Hui Liu Fengping Peng Zhaoyun Liu Kai Ding Jia Song Lijuan Li Jin Chen Qing Shao Siyang Yan Kim De Veirman Karin Vanderkerken Rong Fu Department of Hematology Tianjin Medical University General HospitalTianjin 300052China Department of Hematology and Immunology-Myeloma Center Brussels Vrije Universiteit BrusselBrussels 1090Belgium

Objective:Myeloma bone disease(MBD)is the most common complication of multiple myeloma(MM).Our previous study showed that the serum levels of C3/C4 in MM patients were significantly positively correlated with the severity of bone ***,the mechanism of C3 a/C4 a in osteoclasts MM patients remains ***:The formation and function of osteoclasts were analyzed after adding C3 a/C4 a in ***-seq analysis was used to screen the potential pathways affecting osteoclasts,and the results were verified by Western blot,q RT-PCR,and pathway ***:The osteoclast area per view induced by 1μg/m L(mean±SD:50.828±12.984%)and 10μg/m L(53.663±12.685%)of C3 a was significantly increased compared to the control group(0μg/m L)(34.635±8.916%)(P<0.001 and P<0.001,respectively).The relative m RNA expressions of genes,OSCAR/TRAP/RANKL/cathepsin K,induced by 1μg/m L(median:5.041,3.726,1.638,and 4.752,respectively)and 10μg/m L(median:5.140,3.702,2.250,and 5.172,respectively)of C3 a was significantly increased compared to the control group(median:3.137,2.004,0.573,and 2.257,respectively)(1μg/m L P=0.001,P=0.003,P<0.001,and P=0.008,respectively;10μg/m L:P<0.001,P=0.019,P<0.001,and P=0.002,respectively).The absorption areas of the osteoclast resorption pits per view induced by 1μg/m L(mean±SD:51.464±11.983%)and 10μg/m L(50.219±12.067%)of C3 a was also significantly increased(33.845±8.331%)(P<0.001 and P<0.001,respectively)compared to the *** was no difference between the C4 a and control ***-seq analysis showed that C3 a promoted the proliferation of osteoclasts using the phosphoinositide 3-kinase(PI3 K)signaling *** relative expressions of PIK3 CA/phosphoinositide dependent kinase-1(PDK1)/serum and glucocorticoid inducible protein kinases(SGK3)genes and PI3 K/PDK1/p-SGK3 protein in the C3 a group were significantly higher than in the control *** activation role of C3 a in osteoclasts of MM patients was reduced by the SGK inhibi

关键词： Multiple myeloma complement C3a osteoclasts PI3K/PDK1/SGK3 pathways SGK inhibitor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The action mechanisms and structures designs of F-containing functional materials for high performance oxygen electrocatalysis

引用

Journal of Energy Chemistry 2023年第1期76卷 377-397,I0010页

作者： Gang Wang Shuwei Jia Hongjing Gao Yewen Shui Jie Fan Yixia Zhao Lei Li Weimin Kang Nanping Deng Bowen Cheng State Key Laboratory of Separation Membranes and Membrane Processes School of Textile Science and EngineeringTiangong UniversityTianjin 300387China School of Material science and Engineering Tiangong UniversityTianjin 300387China

Non-renewable fossil fuels have led to serious problems such as global warming,environmental pollution,*** electrocatalysis including oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)plays a central role in clean energy conversion,enabling a number of sustainable processes for future air battery ***,as the most electronegative element(4.0)not only can induce more efficient regulation for the electronic structure,but also can bring more abundant defects and other novel effects in materials selection and preparation for favorable catalysis with respect to the other nonmetal ***,an individual and comprehensive overview of fluorine-containing functional materials for oxygen electrocatalysis field is still ***,it is very meaningful to review the recent progresses of fluorine-containing oxygen *** this review,we first systematically summarize the controllable preparation methods and their possible development directions based on fluorine-containing materials from four preparation *** to the strong electron-withdrawing properties of fluorine,its control of the electronic structure can effectively enhance the oxygen electrocatalytic activity of the *** addition,the catalytic enhancement effect of fluorine on carbonbased materials also includes the prevent oxidation and the layer peeling,and realizes the precise atomic *** the catalytic improvement mechanism of fluorine containing metal-based compounds also includes the hydration of metal site,the crystal transformation,and the oxygen vacancy ***,based on their various dimensions(0D–3D),we also have summarized the advantages of different morphologies on oxygen electrocatalytic ***,the prospects and possible future researching direction of F-containing oxygen electrocatalysts are presented(e.g.,novel pathways,advanced methods for measurement and simulation,field assistance and multi-functions).

关键词： Fluorine-containing functional materials Action mechanisms and structure designs Density functional theory Oxygen evolution reaction Oxygen reduction reaction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：