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Transcriptome Analysis of Ganoderma pseudoferreum of Hevea brasiliensis

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Transcriptome Analysis of Ganoderma pseudoferreum of Hevea b...

Reinforcement of natural rubber latex with silica modified by cerium oxide:preparation and properties

中国植物病理学会2016年学术年会

作者： TU Min CAI Hai-bin CHENG Han HUANG Hua-sun rubber research Institute CATAS

Ganoderma pseudoferreum was the pathogen of hevea red root disease,which generally occurred in the rubber tree planting areas in China and brought huge losses to the rubber *** order to explore the pathogenic mechanism of ***,cDNA library was sequenced and analyzed with the mycelium pure cultured by PDA medium and infected rubber tree root for 30 d respectively,by the Illumina hiseq 2 500 high-throughput sequencing *** results showed that the valid data were 46 024 635 after *** were 6 892 differently expressed genes（q ＜ 0.05 ＆ |log2（Fold-change） | ＞ 1）,3 030 up-regulated genes,3 862 down-regulation of *** up-regulated genes,there were 32 genes which log2（Fold-change） greater than 6 and 13 genes which greater *** up-regulated genes mainly included cellulose,hemicellulase,oxidase,dehydrogenase and transcription *** results lay a foundation for further research on the pathogenic molecular mechanism of ***.

关键词： Ganoderma pseudoferreum Transcriptome Infection Cellulose

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Journal of Rare Earths 2016年第2期34卷 221-226页

作者：张福全廖禄生王永周汪月琼黄红海李普旺彭政曾日中 Chinese Agricultural Ministry Key Laboratory of Tropical Crop Product Processing Agricultural Product Processing Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiang 524001China Agriculture Ministry Key Laboratory of Biology and Genetic Resources of Rubber TreeRubber Research InstituteChinese Academy of Tropical Agricultural SciencesDanzhou 571731China

Variable masses of nano cerium oxide (CeO2) were added into nano silica (SiO2) to prepare the well-dispersed SiO2-CeO2 suspension (SiO2-CeO2), cetyltrimethyl ammonium bromide (CTAB) was used to adjust the compatibility of SiO2-CeO2 with rubber matrix, then SiO2-CeO2 modified by CTAB and curing formulas were mixed with fresh natural rubber (NR) latex to prepare NR/SiO2-CeO2 nanocomposites that contained 0–10 parts of CeO2 by a new emulsion compounding method. The morphologies, cure characteristics, mechanical properties and thermal-oxidative stability of NR/SiO2-CeO2 nanocomposites were investigated. The re-sults revealed that the presence of CeO2 in NR/SiO2-CeO2nanocomposites was favorable for enhancing the interaction between NR matrix and fillers, helped to get smaller SiO2-CeO2 particles with narrower particle size distribution, further improved the crosslink densities and mechanical properties of NR/SiO2-CeO2 nanocomposites vulcanizates. Meanwhile, the addition of CeO2 increased the active energy at least 4.66%, obviously improved the thermal-oxidative aging-inhibiting properties of NR/SiO2-CeO2 nanocomposites. Additionally, nanocomposites containing CeO2 promotedTg shift to high temperature direction, causing the nanocomposites featured higher tanδ at 0 oC and lower tanδ at 60 oC and exhibited comparable wet grip and lower rolling resistance when NR/SiO2-CeO2nano-composites were used in tire tread compound.

关键词： cerium oxide silica natural rubber latex emulsion compound reinforcement aging-inhibiting property glass transition temperature rare earths

Thermal degradation of epoxidized natural rubber in presence of neodymium stearate

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Journal of Rare Earths 2013年第5期31卷 526-530页

作者：罗勇悦杨昌金陈帮乾许逵钟杰平彭政吕臻王庆煌 Chinese Agricultural Ministry Key Laboratory of Tropical Crop Product Processing Agricultural Product Processing Research InstituteChinese Academy of Tropical Agricultural Sciences rubber research Institute Chinese Academy of Tropical Agricultural Sciences School of Science Guangdong Ocean University

The effect of neodymium stearate （NdSt） synthesized by saponification method on thermal degradation and thermo-oxidative degradation of expoxidized natural rubber with 25 mol.% epoxidation （ENR25） was investigated by thermogravimetric analysis （TGA）, and the structure of ENR25 vulcanized with NdSt after thermo-oxidative decomposition was characterized using Fourier transform infared spectroscopy-attenuated total-reflectance （FTIR-ATR）. The thermal degradation kinetic parameters of ENR25 with different loadings of NdSt were determined by Coats-Redfern method. The results showed that the thermal degradation of ENR25 in nitrogen was a one-step reaction regardless of NdSt content, whereas the thermo-oxidative degradation was a multiple-step reaction. The thermal and thermo-oxidative stability of the ENR25 vulcanizates with the addition of NdSt was higher than that of pure ENR25 vulcanizates. The ENR25 vulcanizates with the incorporation of 1 phr （per hundred parts of rubber） NdSt imparted the highest activa- tion energy （E） of thermal and thermo-oxidative degradation. This could be attributed to the many unoccupied orbits in rare earth Nd, which could capture the free radicals and make the epoxide groups stable in the process of thermal and thermo-oxidative degradation for ENR25.

关键词： neodymium stearate ENR25 thermal degradation thermo-oxidative degradation kinetic rare earths 1

Effects of Maize-Soybean Intercropping on Nitrous Oxide Emissions from a Silt Loam Soil in the North China Plain

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Pedosphere 2019年第6期29卷 764-772页

作者： HUANG Jianxiong SUI Peng GAO Wansheng CHEN Yuanquan College of Agriculture and Biotechnology China Agricultural UniversityNo.2 Yuanmingyuan West RoadHaidian DistrictBeijing 100193(China rubber research Institute Chinese Academy of Tropical Agricultural SciencesDanzhou 571737(China

Maize ( Zea mays L.), a staple crop in the North China Plain, contributing substantially to agricultural nitrous oxide (N 2 O) emissions in this region. Many studies have focused on various agricultural management measures to reduce N 2 O emissions. However, few have investigated soil N 2 O emissions in intercropping systems. In the current study, we investigate whether maize-soybean intercropping treatments could reduce N 2 O emission rates. Two differently configured maize-soybean intercropping treatments, 2:2 intercropping (two rows of maize and two rows of soybean, 2M2S) and 2:1 intercropping (two rows of maize and one row of soybean, 2M1S), and monocultured maize (M) and soybean (S) treatments were performed using a static chamber method. The results showed no distinct yield advantage for the intercropping systems. The total N 2 O production from the various treatments was 0.15 ± 0.04–113.85 ± 12.75 µg m −2 min −1 . The cumulative N 2 O emission from the M treatment was 16.9 ± 2.3 kg ha −1 over the entire growing season (three and a half months), which was significantly higher ( P < 0.05) than that of the 2M2S and 2M1S treatments by 36.6% and 32.2%, respectively. Two applications of nitrogen (N) fertilizer (as urea) at 240 kg N ha −1 each induced considerable soil N 2 O fluxes. Short-term N 2 O emissions (within one week after each of the two N applications) accounted for 74.4%–83.3% of the total emissions. Soil moisture, temperature, and inorganic N were significantly correlated with soil N 2 O emissions ( R 2 = 0.246–0.365, n = 192, P < 0.001). Soil nitrate (NO − )3 and moisture decreased in the intercropping treatments during the growing season. These results indicate that maize-soybean intercropping can reduce soil N 2 O emissions relative to monocultured maize.

关键词： Climate change Denitrification Intercropping system Land equivalent ratio Nitrification Nitrous oxide flux Soil temperature Soil water-filled pore space

The secondary laticifer differentiation in rubber tree is induced by trichostatin A, an inhibitor of histone acetylation

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 357-362页

作者： Shixin ZHANG Shaohua WU Weimin TIAN Key Laboratory of Biology and Genetic Resources of rubber Tree Ministry of Agriculture State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops Rubber Research InstituteChinese Academy of Tropical Agricultural Sciences

The secondary laticifer, a specific tissue in the secondary phloem of rubber tree, is differentiated from the vascular cambia. The number of the secondary laticifer in the trunk bark of rubber tree is positively correlated with rubber yield. Although jasmonates have been demonstrated to be crucial in the regulation of secondary laticifer differentiation, the mechanism for the jasmonate-induced secondary laticifer differentiation remains to be *** using an experimental morphological technique, the present study revealed that trichostatin A(TSA), an inhibitor of histone deacetylation, could induce the secondary laticifer differentiation in a concentrationdependent manner. The results suggest that histone acetylation is essential for the secondary laticifer differentiation in rubber tree.

关键词： Hevea brasiliensis histone acetylation laticifer differentiation trichostatin vascular cambia

Factors Influencing Induction of Agrobacterium tumefaciens Virulence Genes

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Agricultural Biotechnology 2012年第5期1卷 34-39页

作者： Zhi ZOU Key Laboratory of Biology and Genetic Resources of rubber Tree Ministry of Agriculture/Rubber Research Institute (RRI)Chinese Academy of Tropical Agricultural Sciences(CATAS)Danzhou

Agrobacterium species are routinely employed for plant genetic modification due to the relatively simple procedures, cost-competitiveness, low copy num- ber, independence to vector DNAs, and targeted integration into transcriptionally active regions of plant chromosomes with defined T-DNA. However, to date, there are still a great number of plant species reluctant to Agrobacterium-mediated transformation. Evidence suggests that the infection capability of Agrobacterium is deter- mined by virulence （vir） genes of Ti plasmid outside ofA. tumefaciens chromosome. Among all v/r genes, virA and virG are constitutively expressed, while the ex- pression of other vir genes is induced by phenolic compounds. In addition, carbohydrates can enhance vir induction mediated by phenolic compounds, while low phosphate and acidic pH conditions may also enhance the induction of vir genes. To improve Agrobacterium-mediated transformation efficiency for potential applica- tions in research and industry, molecular mechanisms of vir induction by factors such as phenolic compounds, carbohydrates, low phosphate, acidic pH and incuba- tion temperature are discussed in this review.

关键词： Agrobacterium tumefaciens Virulence genes virA virG Induced expression

影响磷矿粉在淹水水稻土中溶解和有效性因素研究(英文)

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中国农学通报 2011年第12期27卷 134-139页

作者：郭海超王光火吴小平中国热带农业科学院橡胶研究所海南儋州571737 浙江大学环境与资源学院资源科学系杭州310029

通过对在采自浙江省金华市的12种不同性质的水稻土添加江西省吴村磷矿粉(WPR)进行淹水培养试验,研究淹水条件下影响磷矿粉溶解和有效性的土壤因素。结果表明,磷矿粉在土壤中的溶解量与土壤pH(KCl)、交换性钙离子含量以及土壤粉粒含量呈... 详细信息

通过对在采自浙江省金华市的12种不同性质的水稻土添加江西省吴村磷矿粉(WPR)进行淹水培养试验,研究淹水条件下影响磷矿粉溶解和有效性的土壤因素。结果表明,磷矿粉在土壤中的溶解量与土壤pH(KCl)、交换性钙离子含量以及土壤粉粒含量呈极显著或显著的负相关,而与土壤粘粒含量呈显著的正相关。经逐步回归发现,土壤pH(KCl)是影响磷矿粉溶解的第一因素,土壤pH(KCl)和土壤磷吸附常数K共同解释了78%的磷矿粉溶解。加入磷矿粉后土壤有效磷的增加量与土壤的Olsen-P含量呈极显著的正相关。施用磷矿粉后土壤有效磷增加量占溶解量的百分比与土壤Olsen-P含量呈极显著性正相关,而与土壤粘粒含量、最大吸磷量呈显著负相关。

关键词：有效磷溶解水稻土磷矿粉

Fluxes Footprint and Source Area of rubber Plantation

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Agricultural Science & Technology 2011年第12期12卷 1937-1942页

作者：吴志祥谢贵水陈帮乾杨川周兆德陶忠良农业部儋州热带农业资源与生态环境重点野外科学观测试验站海南儋州571737 中国热带农业科学院橡胶研究所海南儋州571737 海南大学海南海口571737

[Objective] The paper was to analyze the quality of flux observation data of rubber plantation.[Method]Based on the FSAM model,footprint and flux source area were analyzed according to the continuous flux measurement with the open-path eddy covariance system on the 50 m tower of Danzhou Key Field Station of Observation and research for Tropical Agricultural Resources and Environments,Ministry of Agriculture from Jan 1 to Jun 30,2010.[Result] Under unstable stratification,source areas were smaller than those under stable conditions,and source areas in the dormant season were larger than those in the growing season at the same *** the main wind direction 130°-270°,the upwind range of source areas was in the magnitude of 100-758 m and vertical upwind range was-251-251 m at 80% level under unstable stratification in the growing season,and they were some large than those under the unstable stratification in the dormant *** source areas of the upwind and vertical upwind ranges were 173-1 858,-534-534 m especially under stable stratification in the growing season,and they were smaller than those under stable stratification in the dormant *** the other wind directions of 0°-130° and 270°-360°,the ranges were similar to those of the growing season in the prevailing wind direction under the same atmospheric conditions.[Conclusion] The study would lay a foundation for the future flux calculation and analysis.

关键词： Flux footprint FSAM(the Flux-Source Area Model) Flux source area rubber plantation(forest) Hainan Island

Effects of Micro-tapping with Different Concentration of Ethylene on Yield and Physiological Features of rubber Tree(Hevea brasiliensis) Clone PR107

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Agricultural Science & Technology 2015年第5期16卷 873-876,935页

作者：仇键杨文凤吴明魏芳校现周罗世巧农业部橡胶树生物学与遗传资源利用重点实验室/中国热带农业科学院橡胶研究所海南儋州571737

In this study, the rubber tree clone PR107 was selected as test material,and its yields and physiological features under micro-tapping with different concentrations of ethylene were compared. The results showed that there was no significant correlation between rubber yield and ethylene concentration applied by microtapping on PR107. Too high level of ethylene was not conducive to improvement of rubber yield. When the ethylene concentration for micro-tapping was higher than60%, physical deficiencies appeared in rubber tree PR107, the dry rubber content was reduced, and the yield was unstable. Instead, when the ethylene concentration was lower than 40%, the physiological features of PR107 were steady, the dry rubber content was stable, and the yield was relatively high.

关键词： Micro-tapping Physiological features of latex Ethylene concentration Hevea brasiliensis PR107