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CircRNA: a rising star in plant biology

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Journal of Genetics and Genomics 2022年第12期49卷 1081-1092页

作者： Pei Zhang Mingqiu Dai National key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanHubei 430070China

Circular RNAs(circRNAs) are covalently closed single-stranded RNA molecules, which are widespread in eukaryotic cells. As regulatory molecules, circRNAs have various functions, such as regulating gene expression, binding mi RNAs or proteins, and being translated into proteins, which are important for cell proliferation and cell differentiation, individual growth and development, as well as many other biological processes. However, compared with that in animal models, studies of circRNAs in plants lags behind and,particularly, the regulatory mechanisms of biogenesis and molecular functions of plant circRNAs remain elusive. Recent studies have shown that circRNAs are wide spread in plants with tissue-or developmentspecific expression patterns and are responsive to a variety of environmental stresses. In this review, we summarize these advances, focusing on the regulatory mechanisms of biogenesis, molecular and biological functions of circRNAs, and the methods for investigating circRNAs. We also discuss the challenges and the prospects of plant circ RNA studies.

关键词： Plant circRNA circRNA biogenesis circRNA regulation Roles of circRNA Methodologies in circRNA studies

Microstructure and magnetocaloric properties of partially crystallized Gd_(60)Co_(30)Fe_(10)amorphous alloy prepared by different solidification cooling rates

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Rare Metals 2022年第1期41卷 246-253页

作者： Hui-Yan Zhang Zi-Yang Zhang Ya-Fang Xu Ai-Lin Xia Wei-Huo Li Fa-Chao Wang Shuang-Shuang Chen Gerard Sisó key Laboratory of Green Fabrication and Surface Technology of Advanced Metal Materials(Anhui University of Technology) Ministry of Education School of Materials Science and Engineering Anhui University of Technology Departament d’Enginyeria Electro`nica-Escola d’Enginyeria Universitat Autònoma de Barcelona

The Gd_(60)Co_(30)Fe_(10)alloy ribbons with different solidification cooling rates were prepared by modifying the melt-spinning speed of 6.0,12.5,25.0 and 50.0 m·s^(-1)·With cooling rate decreasing,the(Fe,Co)5 Gd and hcp-Gd nanocrystalline was in situ precipitated among the amorphous matrix,which resulted in the composition change of the amorphous *** of the only slight amount of crystalline phase in Gd_(60)Co_(30)Fe_(10)alloys,the magnetic and magnetocaloric properties mainly depend on the amorphous phase,and all the magnetic entropy change versus temperature(|Δ|SMI-T)curves are table-like,indicating the suitability for Ericsson *** magnetic transition temperature of the Gd_(60)Co_(30)Fe_(10)alloy at a melt-spinning speed of 6.0 m·s^(-1) shifted obviously to the lower value with the applied magnetic fields *** peak value of magnetic entropy change(|ΔSMpk|)is 2.19 J·kg^(-1)·K^(-1)at217 K under the magnetic field change of 0-2 T,and the table-like region is 200-230 *** was proved that the moderate reduction of the cooling rate will not deteriorate the magnetocaloric performance of the Gd_(60)Co_(30)Fe_(10)ribbons seriously.

关键词： Magnetic refrigeration Amorphous-nanocrystalline composite In situ precipitation Magnetocaloric effect

Process monitoring of the Au-S bond conversion in acetylene hydrochlorination

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Chinese Journal of Chemical Engineering 2022年第5期35卷 32-40页

作者： Lutai Song Li Liu Mingyuan Zhu Bin Dai School of Chemistry and Chemical Engineering Shihezi UniversityShihezi 832000China key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan Shihezi 832000China

The continuous expansion of vinyl chloride production increases environmental pollution caused by mercury catalysts,which is an issue that urgently needs to be *** and stable catalysts should be researched to alleviate this *** this research,Thiolactic acid acts as a ligand where sulfhydryl groups form a stable complex with Au on the surface of a spherical activated carbon(SAC).An Au-thiolactic acid/SAC catalyst was designed with a Au theoretical loading of 0.5%(mass)to overcome the disadvantages of traditional Au-based catalysts,such as a low conversion rate and poor life *** ratio of Au to ligand was screened,and the activity was best when Au/S=1:*** formation of the Au-S bond was proven by FT-IR and *** longevity test of the Au1 S8/SAC catalyst was carried out at 1200 h^(-1) for 50 *** with reaction times of 0 h,5 h,10 h,20 h,and 50 h were taken to monitor the catalyst *** XPS and TPR tests proved that the Au-S bond broke as the acetylene hydrochlorination reaction proceeded,The DFT calculation proved that the Au-S bond is the active site,and the sulfur atom promotes the adsorption of C_(2)H_(2) by the catalyst.

关键词： Acetylene hydrochlorination Au-S bond Process monitoring Valence shift

Efficient electrocatalytic conversion of N_(2) to NH_(3) using oxygen-rich vacancy lithium niobate cubes

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Chinese Journal of Chemical Engineering 2023年第10期62卷 132-138页

作者： Shuhui Fan Qi Wang Yanan Hu Qiang Zhao Jinping Li Guang Liu Shanxi key Laboratory of Gas Energy Efficient and Clean Utilization College of Chemical Engineering and TechnologyTaiyuan University of TechnologyTaiyuan 030024China

Instead of the energy-intensive Haber-Bosch process,the researchers proposed a way to produce ammonia using water and nitrogen as feedstock,powered by electricity,without polluting the ***,how to design efficient electrocatalyst for electrocatalytic nitrogen reduction reaction(NRR)is still urgent and ***,a strategy is proposed to adjust the morphology and surface electronic structure of electrocatalyst by optimizing material synthesis ***3(lithium niobate,LN)cubes with oxygen-rich vacancy and regular morphology were synthesized by hydrothermal synthesis and followed molten salt calcination process,which were used for electrocatalytic NRR under mild *** with LN nanoparticles synthesized by solid phase reaction,LN cubes exhibit better NRR performance,with the highest ammonia yield rate(13.74μg.h^(-1).mg^(-1))at the best potential of-0.45V(*** hydrogen electrode,RHE)and the best Faradaic efficiency(85.43%)at-0.4 ***,LN cubes electrocatalyst also demonstrates high stability in 7 cycles and 18 h current-time *** investigation of the reaction mechanism confirmed that the structure of oxygen vacancy could adjust the electronic structure of the electrocatalyst,which was conducive to the adsorption and activation of N_(2) molecule and also increased the ECSA of electrocatalyst,thus providing more active sites for the NRR process.

关键词： Nitrogen reduction reaction Ammonia yield rate Oxygen vacancy LiNbO_(3)cubes Electronic structure

A small knottin-like peptide negatively regulates in wheat to stripe rust resistance during early infection of wheat

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The Crop Journal 2023年第2期11卷 457-467页

作者： Shuangyuan Guo Min Li Huankun Li Feng Zhang Qiong Zhang Xueling Huang Xing Li Xiaojie Wang Zhensheng Kang Xinmei Zhang College of Life Sciences Northwest A&F UniversityYangling 712100ShaanxiChina College of Plant Protection Northwest A&F UniversityYangling 712100ShaanxiChina state key Laboratory of Crop Stress Biology for Arid Areas Yangling 712100ShaanxiChina

Although Blufensins(Bln)have important functions in the response of plants to biotic stress the precise functioning of Bln in wheat remains largely *** we isolated a Bln gene(TaBln4)from Suwon 11 infected by Puccinia striiformis ***(Pst).Expression of TaBln4 increased in host plants at the early stage of infection with a virulent Pst race(CYR31)but was unchanged in response to infection by an avirulent race(CYR23).Transcription levels of TaBln4 were also regulated by hormone and abiotic *** of TaBln4 in tobacco leaves suppressed Bax-induced programmed cell *** of TaBln4 by virus-induced gene silencing inhibited colonization of race CYR31 by increasing the accumulation of H2O2 and formation of hypersensitive responses(HR).Transient overexpression of TaBln4 by a transient overexpression system(BSMV-VOX)increased the susceptibility of wheat to *** from bimolecular fluorescence complementation and pull-down assays demonstrated that TaBLN4 interacted with *** together,our results suggest that TaBln4 negatively regulates resistance in wheat to Pst in a reactive oxygen species(ROS)-and HR-dependent manner.

关键词： HR Hypersensitivity Puccinia striiformis f.sp.tritici Triticum aestivum Virus-induced gene silencing

Natural siderite derivatives activated peroxydisulfate toward oxidation of organic contaminant: A green soil remediation strategy

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Journal of Environmental Sciences 2023年第5期127卷 615-627页

作者： Chengwei Zhong Yinying Jiang Quanfeng Liu Xiaoshuang Sun Jiang Yu Department of Environmental Science and Engineering College of Architecture and EnvironmentSichuan UniversityChengdu 610065China Yibin Institute of Industrial Technology Sichuan UniversityYibin 644000China Institute of New Energy and Low Carbon Technology Sichuan UniversityChengdu 610065China

Natural siderite(FeCO_(3)),simulated synthetic siderite and nZVI/FeCO_(3) composite were used as green and easily available iron-based catalysts in peroxydisulfate activation for remediating 2-chlorophenol as the target contaminant and this technique can effectively degrade organic pollutants in the *** key reaction parameters such as catalysts dosage,oxidant concentration and pH,were investigated to evaluate the catalytic performance of different materials in catalytic *** buffering property of natural soil conduced satisfactory degradation performance in a wide pH range(3-10).Both the main non-radical of ^(1)O_(2) and free radicals of SO_(4)^(-) and OH·were evidenced by quenching experiment and electron paramagnetic *** reduction of nZVI on FFC surface not only has the advantage for electronic transfer to promote the circulation of Fe(Ⅲ)to Fe(Ⅱ),but also can directly ***,the intermediates were comprehensively analyzed by GC-MS and a potential removal mechanism of three oxidant system for 2-CP soil degradation was ***,this research provides a new perspective for organic contaminate soil treatment using natural siderite or simulated synthetic siderite as efficient and environmental catalytic material.

关键词： Natural siderite 2-chlorophenol Soil Peroxydisulfate Reactive radicals

Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury

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Signal Transduction and Targeted Therapy 2022年第4期7卷 1155-1169页

作者： Chen Li Zhourui Wu Liqiang Zhou Jingliang Shao Xiao Hu Wei Xu Yilong Ren Xingfei Zhu Weihong Ge Kunshan Zhang Jiping Liu Runzhi Huang Jing Yu Dandan Luo Xuejiao Yang Wenmin Zhu Rongrong Zhu Changhong Zheng Yi Eve Sun Liming Cheng Division of Spine Department of OrthopaedicsTongji HospitalTongji University School of MedicineShanghai 200065China key Laboratory of Spine and Spinal cord Injury Repair and Regeneration(Tongji University) Ministry of EducationShanghai 200072China Institute of Spinal and Spinal Cord Injury Tongji University School of MedicineShanghai 200065China Stem Cell Translational research Center Tongji HospitalTongji University School of MedicineShanghai 200065China Department of Psychiatry and Biobehavioral Sciences David Geffen School of MedicineUniversity of California Los AngelesLos AngelesCA 90095USA

Spinal cord injury(SCI)involves diverse injury responses in different cell types in a temporally and spatially specific ***,using single-cell transcriptomic analyses combined with classic anatomical,behavioral,electrophysiological analyses,we report,with single-cell resolution,temporal molecular and cellular changes in crush-injured adult mouse spinal *** revealed pathological changes of 12 different major cell types,three of which infiltrated into the spinal cord at distinct times *** discovered novel microglia and astrocyte subtypes in the uninjured spinal cord,and their dynamic conversions into additional stage-specific subtypes/*** dynamic changes occur at 3-days post-injury and by day-14 the second wave of microglial activation emerged,accompanied with changes in various cell types including neurons,indicative of the second round of *** day-38,major cell types are still substantially deviated from uninjured states,demonstrating prolonged *** study provides a comprehensive mapping of cellular/molecular pathological changes along the temporal axis after SCI,which may facilitate the development of novel therapeutic strategies,including those targeting microglia.

关键词： alterations injury pathological

A Local Quadratic Embedding Learning Algorithm and Applications for Soft Sensing

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Engineering 2022年第11期18卷 186-196页

作者： Yaoyao Bao Yuanming Zhu Feng Qian key Laboratory of Smart Manufacturing in Energy Chemical Process Ministry of EducationEast China University of Science and TechnologyShanghai 200237China

Inspired by the tremendous achievements of meta-learning in various fields,this paper proposes the local quadratic embedding learning(LQEL)algorithm for regression problems based on metric learning and neural networks(NNs).First,Mahalanobis metric learning is improved by optimizing the global consistency of the metrics between instances in the input and output ***,we further prove that the improved metric learning problem is equivalent to a convex programming problem by relaxing the *** on the hypothesis of local quadratic interpolation,the algorithm introduces two lightweight NNs;one is used to learn the coefficient matrix in the local quadratic model,and the other is implemented for weight assignment for the prediction results obtained from different local ***,the two sub-mod els are embedded in a unified regression framework,and the parameters are learned by means of a stochastic gradient descent(SGD)*** proposed algorithm can make full use of the information implied in target labels to find more reliable reference ***,it prevents the model degradation caused by sensor drift and unmeasurable variables by modeling variable differences with the LQEL *** results on multiple benchmark datasets and two practical industrial applications show that the proposed method outperforms several popular regression methods.

关键词： Local quadratic embedding Metric learning Regression machine Soft sensor

Dynamic simulation of the tractor HMCVT under typical working conditions based on AMESim

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international Journal of Agricultural and Biological Engineering 2022年第2期15卷 102-110页

作者： Maohua Xiao Yuanfang Zhao Xianhua Li Ghulam Hussain Shengjie Wang Yejun Zhu College of Engineering Nanjing Agricultural UniversityNanjing 210031China key Laboratory of Modern Manufacturing Technology Guizhou UniversityGuiyang 550025China Faculty of Mechanical Engineering Ghulam Ishaq Khan Institute of Engineering Sciences&TechnologyTopi23460Pakistan Jiangsu Yueda Intelligent Agricultural Equipment Co. Ltd.Yancheng 224004JiangsuChina

In order to study the dynamic characteristics of hydro-mechanical continuously variable transmission(HMCVT)under ploughing and sowing conditions,a complete simulation model of HMCVT is established based on AMESim software,including mechanical transmission model,pump controlled hydraulic motor speed control model and section changing hydraulic system *** addition,the dynamic model of tractor is *** order to verify the correctness of the simulation model,a test-bed is *** test of tractor running speed and the test of pump controlled hydraulic motor system were carried out on the *** test results show that the simulation model of pump control hydraulic system can correctly reflect the change of transmission ratio of pump controlled hydraulic motor,and the simulation model can reflect the actual working condition change of ***,the correctness of the previous simulation model based on AMESim is *** on the simulation model established by AMESim,the dynamic characteristics of HMCVT under ploughing and sowing conditions are *** results show that:Under ploughing condition,the planetary platoon will have strong impact at the moment of throttle opening and changing *** sowing condition,the HMCVT will have a great impact at the time of variable cross section,but the variation range of rodent force decreases and the changing trend tends to be stable.

关键词： tractor HMCVT AMESim dynamics simulation test