A small knottin-like peptide negatively regulates in wheat to stripe rust resistance during early infection of wheat

作     者：Shuangyuan Guo Min Li Huankun Li Feng Zhang Qiong Zhang Xueling Huang Xing Li Xiaojie Wang Zhensheng Kang Xinmei Zhang Shuangyuan Guo;Min Li;Huankun Li;Feng Zhang;Qiong Zhang;Xueling Huang;Xing Li;Xiaojie Wang;Zhensheng Kang;Xinmei Zhang

作者机构：College of Life SciencesNorthwest A&F UniversityYangling 712100ShaanxiChina College of Plant ProtectionNorthwest A&F UniversityYangling 712100ShaanxiChina State Key Laboratory of Crop Stress Biology for Arid AreasYangling 712100ShaanxiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第2期

页      面：457-467页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 0902[农学-园艺学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Key Research and Development Program of China(2021YFD1401000) the International Science and Technology Cooperation Project of Shaanxi Provincial Key R&D Plan-Key Project(2020KWZ-009) the Shaanxi Innovation Team Project(2018TD-004) the 111 Project of the Ministry of Education of China(B07049) 

主　　题：HR Hypersensitivity Puccinia striiformis f.sp.tritici Triticum aestivum Virus-induced gene silencing 

摘      要：Although Blufensins(Bln)have important functions in the response of plants to biotic stress the precise functioning of Bln in wheat remains largely *** we isolated a Bln gene(TaBln4)from Suwon 11 infected by Puccinia striiformis ***(Pst).Expression of TaBln4 increased in host plants at the early stage of infection with a virulent Pst race(CYR31)but was unchanged in response to infection by an avirulent race(CYR23).Transcription levels of TaBln4 were also regulated by hormone and abiotic *** of TaBln4 in tobacco leaves suppressed Bax-induced programmed cell *** of TaBln4 by virus-induced gene silencing inhibited colonization of race CYR31 by increasing the accumulation of H2O2 and formation of hypersensitive responses(HR).Transient overexpression of TaBln4 by a transient overexpression system(BSMV-VOX)increased the susceptibility of wheat to *** from bimolecular fluorescence complementation and pull-down assays demonstrated that TaBLN4 interacted with *** together,our results suggest that TaBln4 negatively regulates resistance in wheat to Pst in a reactive oxygen species(ROS)-and HR-dependent manner.