检索结果-南通市图书馆

Horticultural Plant Journal 2025年第1期11卷 123-132页

作者： Liyao Su Tian Zhang Zong-Ming(Max)Cheng state key laboratory of crop genetics and germplasm enhancement College of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China

Ascorbic acid, also referred to as vitamin C(Vc), is an important nutrient found in fruits and vegetables that promotes produce quality and human health. Rosa roxburghii is an underutilized natural fruit that contains very high levels of Vc. However, the Vc content of R. roxburghii varies considerably during plant development and ripening. To better understand the molecular mechanisms that underlie fluctuations in Vc content of R. roxburghii fruit at different developmental stages, we performed transcriptomic and metabolomic analyses and identified two significant gene networks/modules and 168 transcription factors directly involved in Vc synthesis. Promoter analysis of two core genes involved in Vc synthesis, RrGGP and RrGalUR, revealed the presence of a retroviral long terminal repeat(LTR) insert in the RrGalUR promoter. Using yeast one-hybrid and dual-luciferase assays, we demonstrated that the transcription factors RrHY5H and RrZIP9 bind to the promoter of RrGGP to promote its expression. RrZIP6 and RrWRKY4 bind to the LTR in the RrGalUR promoter to promote its expression. Our results reveal a molecular mechanism that controls Vc synthesis and accumulation in R. roxburghii fruit.

关键词： Rosa roxburghii Vitamin C WGCNA Transcriptome Metabolome

The dynamic arms race during the early invasion of woodland strawberry by Botrytis cinerea revealed by dual dense high-resolution RNA-seq analyses

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2023年第12期10卷 175-189页

作者： Yibo Bai Haibin Wang Kaikai Zhu Zong-Ming Cheng College of Horticulture Nanjing Agricultural UniversityNanjing 210095China key laboratory of crop Gene Resources and germplasm enhancement in Southern China Ministry of AgricultureTropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China Co-innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjingJiangsu 210037China

Necrotrophic pathogens replicate massively upon colonizing plants,causing large-scale wilting and death of plant ***-standing both mechanisms of pathogen invasion and host response processes prior to symptom appearance and their key regulatory networks is therefore important for defense against pathogen ***,we investigated the mechanisms of interaction between woodland strawberry(Fragaria vesca)leaves and gray mold pathogen(Botrytis cinerea)at 14 infection time points during the first 12 hours of the infection period using a dense,high-resolution time series dual transcriptomic analysis,characterizing the arms race between strawberry *** and *** before the appearance of localized *** leaves rapidly initiated strong systemic defenses at the first sign of external stimulation and showed lower levels of transcriptomic change later in the infection *** the host plants,*** showed larger-scale transcriptomic changes that persisted throughout the infection *** gene co-expression network analysis identified highly correlated genes in 32 gene expression modules between *** and *** two-hybrid and bimolecular fluorescence complementation assays revealed that the disease response protein FvRLP2 from woodland strawberry interacted with the cell death inducing proteins BcXYG1 and BcPG3 from *** of FvRLP2 in both strawberry and Arabidopsis inhibited *** infection,confirming these genes’respective *** findings shed light on the arms race process by which *** invades host plants and strawberry to defend against pathogen infection.

关键词： straw fir invasion

Identification of key genes regulating the synthesis of quercetin derivatives in Rosa roxburghii through integrated transcriptomics and metabolomics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第3期23卷 876-887页

作者： Liyao Su Min Wu Tian Zhang Yan Zhong Zongming(Max) Cheng state key laboratory of crop genetics and germplasm enhancement College of HorticultureNanjing Agricultural UniversityNanjing210095China

Rosa roxburghii fruit is rich in flavonoids, but little is known about their biosynthetic pathways. In this study, we employed transcriptomics and metabolomics to study changes related to the flavonoids at five different stages of R. roxburghii fruit development. Flavonoids and the genes related to their biosynthesis were found to undergo significant changes in abundance across different developmental stages, and numerous quercetin derivatives were identified. We found three gene expression modules that were significantly associated with the abundances of the different flavonoids in R. roxburghii and identified three structural UDP-glycosyltransferase genes directly involved in the synthesis of quercetin derivatives within these modules. In addition, we found that RrBEH4, RrLBD1 and RrPIF8could significantly increase the expression of downstream quercetin derivative biosynthesis genes. Taken together,these results provide new insights into the metabolism of flavonoids and the accumulation of quercetin derivatives in R. roxburghii.

关键词： Rosa roxburghii quercetin derivatives weighted gene co-expression network analysis transcription factor transcriptome metabolome

Evolution of the R2R3-MYB gene family in six Rosaceae species and expression in woodland strawberry

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2019年第12期18卷 2753-2770页

作者： LIU Hui XIONG Jin-song JIANG Yue-ting WANG Li CHENG Zong-ming(Max) College of Horticulture Nanjing Agricultural UniversityNanjing 210095P.R.China Department of Plant Sciences University of TennesseeKnoxville 37996USA

R2R3-MYB gene family play important roles in plants development, metabolism, and responses to various biotic and abiotic stresses. In this study, 838 R2 R3-MYB genes were identified from six Rosaceae species, including 105 in woodland strawberry(Fragaria vesca), 173 in European pear(Pyrus communis), 219 in apple(Malus domestica), 121 in peach(Prunus persica), 121 in Chinese rose(Rosa chinensis), and 99 in black raspberry(Rubus occidentalis). All R2 R3-MYB genes in the six Rosaceae species were clustered into 51 species-specific duplicated clades with 109 genes and 50 lineage-specific duplicated clades with 242 genes according to phylogenetic analysis. R2 R3-MYB genes were distributed on all chromosomes in each of the six species, with a small amount of tandem duplication events. The proportion of tandem repeat genes ranged from 0 to 25.1%. The R2 R3-MYB protein was conserved in a clade and likely to share similar functions. The distribution of Ks showed the duplication times of R2 R3-MYB genes in six Rosaceae species. Furthermore, most of the R2 R3-MYB genes had Ka/Ks values less than 1, which indicated they were driven by purifying selection during the evolutionary processes. The GO term enrichment analysis revealed that R2 R3-MYB genes in strawberry and black raspberry were more divergent than in other Rosaceae species. Analysis of transcriptomes of 42 different tissues and development stages of woodland strawberry showed that high expression levels of R2 R3-MYB suggested that the R2 R3-MYB genes in strawberry played a key role in growth and development of both vegetative tissues and fruits. The strawberry R2 R3-MYB genes in sub-group of S1, S2, S11, S20, and S22 had high expression levels both in young leaves(YL) and old leaves(OL) strawberry tissues under drought treatments.

关键词： R2R3-MYB gene Rosaceae species duplicati on eve nts

Involvement of Protein Phosphorylation in Water Stress-induced Antioxidant Defense in Maize Leaves

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2009年第7期51卷 654-662页

作者： Shucheng Xu Haidong Ding Fengxia Su Aying Zhang Mingyi Jiang College of Life Sciences Nanjing Agricultural University Nanjing 210095 China College of Life Sciences Fuyang Teachers College Fuyang 236032 China National key laboratory of crop genetics and germplasm enhancement Nanjing Agricultural University Nanjing 210095 China

Using pharmacological and biochemical approaches, the role of protein phosphorylation and the interrelationship between water stress-enhanced kinase activity, antioxidant enzyme activity, hydrogen peroxide （H202） accumulation and endogenous abscisic acid in maize （Zea mays L.） leaves were investigated. Water-stress upregulated the activities of total protein phosphorylation and Ca^2＋-dependent protein kinase, and the upregulation was blocked in abscisic aciddeficient vp5 mutant. Furthermore, pretreatments with a nicotinamide adenine dinucleotide phosphate oxidase inhibitor and a scavenger of H2O2 significantly reduced the increased activities of total protein kinase and Ca^2＋-dependent protein kinase in maize leaves exposed to water stress. Pretreatments with different protein kinase inhibitors also reduced the water stress-induced H2O2 production and the water stress-enhanced activities of antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase. The data suggest that protein phosphorylation and H2O2 generation are required for water stress-induced antioxidant defense in maize leaves and that crosstalk between protein phosphorylation and H2O2 generation may occur.

关键词： abscisic acid antioxidant defense hydrogen peroxide protein phosphorylation water stress

Molecular Cloning and Expression Analysis of a FT Homologous Gene from Solanum tuberosum

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第8期9卷 1133-1139页

作者： FAN Chun-yuan YIN Jing-ming WANG Bing ZHANG Yun- feng YANG Qing state key laboratory of crop genetics and germplasm enhancement/College of Life Sciences Nanjing Agricultural University Nanjing 210095 P.R.China Jiangsu key laboratory for Eco-Agricultural Biotechnology Around Hongze Lake Huai 'an 223300 P.R.China

A homologue of flowering locus T gene, designated StFT, was isolated from Solanum tuberosum by reverse transcriptasepolymerase chain reaction （accession no. GU223211）. The DNA sequence of StFT was 1 626 bp long and contained four exons and three introns. The open reading frame of the gene was 522 bp long and encoded a putative protein of 173 amino acids with a molecular weight of 19.75 kD and a theoretical pI of 7.76. StFT protein had a conserved PBP domain and a higher degree of identity with FT homologous members from other species. Analysis on the mRNA levels of StFT showed that it was highly expressed in leaves, apical buds, flowers, and swelling stolons. Further analysis indicated that its expression was regulated by CONSTANS gene in StCOL-antisense transgenic potato plants.

关键词： cloning expression StFT gene Solanum tuberosum

InDel and SNP Markers and Their Applications in Map-based Cloning of Rice Genes

在线全文

学校读者我要写书评

暂无评论

Rice science 2008年第4期15卷 251-258页

作者： Cun-hong PAN[a][b] Ai-hong LI[a] Zheng-yuan DAI[a] Hong-xi ZHANG[a] Guang-qing LIU[a] Zi-bin WANG[b] Yu-yin MA[b] Yue-jun YIN[b] Ya-fang ZHANG[b] Shi-min ZUO[b] Zong-xiang CHEN[b] Xue-biao PAN[b] [a] Lixiahe Agricultural Research Institute of Jiangsu Province Yangzhou 225007 China [b] Jiangsu Provincial key laboratory of crop genetics and Physiology/key laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 China

High-density markers are necessary for map-based cloning of rice genes, but the currently available markers are not satisfactory enough. InDel (insertion-deletion length polymorphism) and SNP (single nucleotide polymorphism) are the new generation of molecular markers and can basically meet the need of fine mapping. InDel and SNP markers can be developed through bioinformatics. These markers are valuable markers with the characters of low cost, high specificity and stability. This article introduced the methods for designing InDel and SNP markers, taking the mapping of a rice rolled leaf gene as an example. In addition, some key factors in improving the design efficiency were also discussed.

关键词： rice molecular marker insertion-deletion length polymorphism single nucleotide polymorphism bioinformatics gene mapping rolled leaf gene

Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2010年第6期9卷 921-927页

作者： ZHANG Fan WANG Zhong HUANG Quan-sheng HUANG Le-ping YANG Qing state key laboratory of crop genetics and germplasm enhancement College of Life Sciences Nanjing Agricultural University Nanjing 210095 P.R. China Institute of Nuclear and Biotechnology Xinjiang Academy of Agricultural Sciences Urmuqi 830091 P.R.China

Verticillium wilt is a severe disease in eggplant caused by Verticillium ***-inhibiting proteins （PGIPs） have been shown to be involved in preventing the invasion of fungus including *** genes encoding PGIPs is quite valuable for plant resistance breeding to Verticillium *** this study,a cDNA encoding the polygalacturonase-inhibiting protein was isolated from Solanum torvum by RT-PCR and RACE,designated StPGIP （accession ***943498）.The cDNA sequence of StPGIP was 1 097 bp long and contained an open reading frame of 990 *** predicted amino acid sequence of the gene consisted of 329 amino acids and had conserved *** StPGIP protein had a high identity with PGIPs from other *** of StPGIP expression at the mRNA level by RT-PCR showed that the gene was expressed in all organs and could be induced to increase expression by *** dahliae infection.

关键词： Solanum torvum StPGIP cloning expression

Genome sequences of horticultural plants:past,present,and future

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 321-343页

作者： Fei Chen Yunfeng Song Xiaojiang Li Junhao Chen Lan Mo Xingtan Zhang Zhenguo Lin Liangsheng Zhang College of Horticulture Nanjing Agricultural UniversityNanjing 210095China College of crop Science Fujian Agriculture and Forestry UniversityFuzhou 350002China state key laboratory of Subtropical Silviculture School of Forestry and BiotechnologyZhejiang A&F UniversityHangzhou 311300China Department of Biology Saint Louis UniversitySt.LouisMO 63103USA Fujian Provincial key laboratory of Haixia Applied Plant Systems Biology and Quality Science and Processing Technology in Special Starch Key Laboratory of Ministry of Education for Genetics&Breeding and Multiple Utilization of CropsCollege of Crop ScienceFuzhouChina

Horticultural plants play various and critical roles for humans by providing fruits,vegetables,materials for beverages,and herbal medicines and by acting as *** have also shaped human art,culture,and environments and thereby have influenced the lifestyles of *** the advent of sequencing technologies,there has been a dramatic increase in the number of sequenced genomes of horticultural plant species in the past *** genomes of horticultural plants are highly diverse and complex,often with a high degree of heterozygosity and a high ploidy due to their long and complex history of evolution and *** we summarize the advances in the genome sequencing of horticultural plants,the reconstruction of pan-genomes,and the development of horticultural genome *** also discuss past,present,and future studies related to genome sequencing,data storage,data quality,data sharing,and data visualization to provide practical guidance for genomic studies of horticultural ***,we propose a horticultural plant genome project as well as the roadmap and technical details toward three goals of the project.

关键词： details thereby sequences

Meta-analysis of the effects of 1-methylcyclopropene(1-MCP)treatment on climacteric fruit ripening

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 53-68页

作者： Jing Zhang Yuanchun Ma Chao Dong Leon A.Terry Christopher B.Watkins Zhifang Yu Zong-Ming(Max)Cheng College of Horticulture Nanjing Agricultural UniversityNanjingChina Plant Science laboratory Cranfield UniversityBedfordshireUK School of Integrative of Plant Science College of Agriculture and Plant SciencesCornell UniversityIthacaNYUSA College of Food Science and Technology Nanjing Agricultural UniversityNanjingChina Department of Plant Sciences University of TennesseeKnoxvilleTN 37996USA Shanghai key laboratory of Protected Horticultural Technology Forestry and Fruit Tree Research InstituteShanghai Academy of Agricultural SciencesShanghaiChina

1-Methylcyclopropene(1-MCP)is an inhibitor of ethylene perception that is widely used to maintain the quality of several climacteric fruits during storage.A large body of literature now exists on the effects of 1-MCP on climacteric fruit ripening for different species and environmental conditions,presenting an opportunity to use meta-analysis to systematically dissect these *** classified 44 ripening indicators of climacteric fruits into five categories:physiology and biochemistry,quality,enzyme activity,color,and ***-analysis showed that 1-MCP treatment reduced 20 of the 44 indicators by a minimum of 22%and increased 6 indicators by at least 20%.These effects were associated with positive effects on delaying ripening and maintaining *** the seven moderating variables,species,1-MCP concentration,storage temperature and time had substantial impacts on the responses of fruit to 1-MCP *** from different species varied in their responses to 1-MCP,with the most pronounced responses observed in rosaceous fruits,especially apple,European pear fruits,and tropical *** effect of gaseous 1-MCP was optimal at 1μl/l,with a treatment time of 12–24 h,when the storage temperature was 0℃for temperate fruits or 20℃for tropical fruits,and when the shelf temperature was 20℃,reflecting the majority of experimental *** findings will help improve the efficacy of 1-MCP application during the storage of climacteric fruits,reduce fruit quality losses and increase commercial value.

关键词： treatment analysis maintain