InDel and SNP Markers and Their Applications in Map-based Cloning of Rice Genes

作     者：Cun-hong PAN[a][b] Ai-hong LI[a] Zheng-yuan DAI[a] Hong-xi ZHANG[a] Guang-qing LIU[a] Zi-bin WANG[b] Yu-yin MA[b] Yue-jun YIN[b] Ya-fang ZHANG[b] Shi-min ZUO[b] Zong-xiang CHEN[b] Xue-biao PAN[b] 

作者机构：[a] Lixiahe Agricultural Research Institute of Jiangsu Province Yangzhou 225007 China [b] Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2008年第15卷第4期

页      面：251-258页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：1Lixiahe Agricultural Research Institute of Jiangsu Province Ministry of Education Yangzhou University State Key Laboratory of Crop Genetics and Germplasm Enhancement 

主　　题：rice molecular marker insertion-deletion length polymorphism single nucleotide polymorphism bioinformatics gene mapping rolled leaf gene 

摘      要：High-density markers are necessary for map-based cloning of rice genes, but the currently available markers are not satisfactory enough. InDel (insertion-deletion length polymorphism) and SNP (single nucleotide polymorphism) are the new generation of molecular markers and can basically meet the need of fine mapping. InDel and SNP markers can be developed through bioinformatics. These markers are valuable markers with the characters of low cost, high specificity and stability. This article introduced the methods for designing InDel and SNP markers, taking the mapping of a rice rolled leaf gene as an example. In addition, some key factors in improving the design efficiency were also discussed.