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The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1

Phyton-International journal of Experimental Botany 2023年第4期92卷 1109-1124页

作者： Yeyao Du Yang Song Ye Zhang Sujie Fan Hanzhu Zhang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun130118China

Polyamines play an important regulatory role during plant growth and development and adversity stress,and polyamine oxidase(PAO)is involved in polyamine *** this study,an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling ***,its expression pattern and function were *** identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt *** increasing stress concentration,the seed germination rate ***,the seed germination rate of the overexpressed lines was significantly higher than that of the control lines,and the phenotypic character of the root systems was also better than that of the control *** measurement of superoxide dismutase(SOD)and peroxidase(POD)activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities,significantly reducing the malondialdehyde *** the hydrogen peroxide content in the transformed plants gradually increased,the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing *** on this,it was preliminarily judged that GmPAO1 can improve soybean salt tolerance.

关键词： Soybean polyamine oxidase salt tolerance overexpressed

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Identification of Resistance to Pathogenesis Related Protein GmPR1L in Tobacco Botrytis cinerea Infection

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Phyton-International journal of Experimental Botany 2023年第6期92卷 1907-1920页

作者： Yeyao Du Ye Zhang Yang Song Zhuo Zhang Sujie Fan Hanzhu Zhang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchunChina Northeast Institute of Geography and Agroecology Chinese Academy of SciencesChangchunChina

Soybean(Glycine max(Linn.)Merr.)annual leguminous crop is cultivated all over the *** occurrence of diseases has a great impact on the yield and quality of *** this study,based on the RNA-seq of soybean variety M18,a complete CDS(Coding sequence)GmPR1L of the pathogenesis-related protein 1 family was obtained,which has the ability to resist fungal *** overexpression vector and interference expression vector were transferred into tobacco NC89,and the resistance of transgenic tobacco(Nicotiana tabacum L.)to Botrytis cinerea infection was *** results show that:Compared with the control,the activities of related defense enzymes SOD(Superoxide dismutase),POD(Peroxidase),PAL(L-phenylalanine ammonia-lyase)and PPO(Polyphenol oxidase)in the over-expressed transgenic tobacco OEA1 and OEA2 increased to different degrees,and increased significantly at different infection time *** activities of defense enzymes in the interfering strains IEA1 and IEA2 were significantly lower than those in the control *** results of resistance level identification showed that the disease spot rate of OEA1 was significantly lower than that of the control line,and the disease spot rate of OEA2 was significantly lower than that of the control *** plaque rate of the interfering expression line IEA1-IEA2 was significantly higher than that of the control *** is preliminarily believed that the process related protein GmPR1L can improve the resistance of tobacco to ***.

关键词： Soybean pathogenesis-related protein defense enzymes Botrytis cinerea

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