检索结果-南通市图书馆

Eliminating viscosity bias in lateral flow tests

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2021年第5期7卷 195-204页

作者： Daniel M.Kainz Bastian J.Breiner Susanna M.Fruh Tobias Hutzenlaub Roland Zengerle Nils Paust Laboratory for MEMS Applications IMTEK-Department of Microsystems EngineeringUniversity of FreiburgGeorges-Koehler-Allee 10379110 FreiburgGermany Hahn-Schickard Georges-Koehler-Allee 10379110 FreiburgGermany

Despite the widespread application of point-of-care lateral flow tests,the viscosity dependence of these assay results remains a significant ***,we employ centrifugal microfluidic flow control through the nitrocellulose membrane of the strip to eliminate the viscosity *** key feature is the balancing of the sample flow into the cassette of the lateral flow test with the air flow out of the cassette.A viscosity-indepen3ent flow rate of 3.01±0.18 min(±6%)is demonstrated for samples with viscosities ranging from 1.1 mPas to 24 mPas,a factor greater than *** a model human IgG lateral flow assay,signal-intensity shifts caused by varying the sample viscosity from 1.1 mPas to 23 mPas could be reduced by more than 84%.

关键词： viscosity flow eliminate

In vivo bioluminescence imaging to elucidate stem cell graft differentiation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration research 2020年第1期15卷 61-62页

作者： Stefanie Vogel Mathias Hoehn Markus Aswendt Technische Universitat Dresden DFG-Research Center for Regenerative Therapies Dresden(CRTD)DresdenGermany Cognitive Neuroscience Institute of Neuroscience and Medicine(INM-3)Research Center JuelichJuelichGermany Leiden University Medical Center Department of RadiologyLeidenThe Netherlands University of Cologne Faculty of Medicine and University Hospital CologneDepartment of NeurologyCologneGermany

Neurological disorders including neurodegeneration(e.g.,Alzheimer’s disease and Parkinson’s disease)and acute injuries(e.g.,stroke and traumatic brain injury)are the leading cause group of disability-adjusted life years and the second leading cause group of *** to other tissues,the adult brain retains only a very limited repair *** neurogenesis,the lifelong generation of new neurons,declines with age and in degenerative diseases,such as Alzheimer’s ***,independently of age,the proliferation and migration of endogenous stem cells is stimulated after brain injuries and might be related to recovery processes(Adamczak et al.,2017).The limited number of endogenous stem cells during adulthood is one of the major limitations for an efficient regeneration of the injury affected brain ***,the transplantation of neural stem or progenitor cells(NSCs/NPCs)is extensively studied in mouse models and applied in first clinical trials with the aim to replace dysfunctional or lost neural cells and thus to restore brain function.

关键词： injuries endogenous replace

Micro-nano structural electrode architecture for high power energy storage

在线全文

学校读者我要写书评

暂无评论

Journal of Semiconductors 2023年第5期44卷 1-6页

作者： Xin Chao Chengzhan Yan Huaping Zhao Zhijie Wang Yong Lei Institute of Nanochemistry and Nanobiology School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444China Fachgebiet Angewandte Nanophysik Institut für Physik&IMN MacroNanoTechnische Universität IlmenauIlmenau98693Germany Key Laboratory of Semiconductor Materials Science Beijing Key Laboratory of Low Dimensional Semiconductor Materials and DevicesInstitute of SemiconductorsChinese Academy of SciencesBeijing 100083China

The necessity and superiorities of micro-nano structural electrodes toward high power:Electrochemical energy storage(EES)technologies have achieved great success in portable electronics and electric vehicles owing to their environmental friendliness and cost *** the promotional concepts such as the Internet of Things and ultra-high efficiency self-powered systems in recent years,there are substantial demand for superior EES systems,including but not limited to high-performance,miniaturization and multifunction[1−4].In a particular EES cell,active materials are carried by electrodes as the basic building blocks of energy storage or *** innovation(includes composition,structure,size and morphology)has revealed remarkable energy density,power density and lifespan for associated devices in the lab setting of low mass loading slurry-coating electrodes[5].

关键词： structural power electrode

Phosphorylation of WHIRLY1 by CIPK14 Shifts Its Localization and Dual Functions in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第5期10卷 749-763页

作者： Yujun Ren Yanyun Li Youqiao Jiang Binghua Wu Ying Miao Center for Molecular Cell and Systems Biology Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology College of Life Sciences Fujian Agriculture and Forestry University Fuzhou 350002 China These authors contributed equally to this article

Plastid-to-nucleus retrograde signaling is critical for normal growth and development in plants. The dualfunction and dual-located ssDNA binding protein WHIRLY1 （WHY1） has been proposed to coordinate the retrograde signaling from plastids to the nucleus. However, the regulatory mechanism governing the functional switch of WHY1 for mediating plastid-to-nucleus retrograde signaling remains unknown. Here, we report that the Calcineurin B-Like-Interacting Protein Kinase14 （CIPK14） interacts with and phosphorylates WHY1 in Arabidopsis. Phosphorylation of WHY1 results in increased accumulation in the nucleus and enhanced binding with the promoter of WRKY53, which encodes a key transcription factor regulating leaf senescence in Arabidopsis. Transgenic plants overexpressing CIPK14 showed an increased nuclear isoform but decreased plastid isoform of WHY1, among which 95% of transgenic lines showed the stay-green phenotype and 5% of lines showed the variegated pale-green phenotype. Interestingly, the phenotypes of both types of transgenic plants could be recovered by overexpression of plastid-form WHY1. In contrast, knockdown of ClPK14 caused early senescence and even seedling-lethal phenotypes along with elevated expression of senescence-related genes such as WRKY53, SAG12, and NDHF but decreased expression of MER11, RAD50, and POR genes, which could be rescued by overexpression of CIPK14 but not by overexpressing plastid-form or nuclear-form WHY1; the stay-green plants overexpressing ClPK14 showed reduced expression of WRKY53, SAG12, NDHF, and large plastid rRNA. Consistently, the accu- mulation of nuclear-form WHY1 was significantly reduced in the CIPK14 knockdown lines, resulting in a low ratio of nuclear-/plastid-form WHY1. Taken together, our results demonstrate that CIPK14 regu- lates the phosphorylation and organeUar distributions of WHY1 and pinpoint that ClPK14 may function as a cellular switch between leaf senescence and plastid development for coordinating the int

关键词： WHIRLY1 CIPK14 Retrograde Signalling Leaf Senescence Plastid Development

Quantifying land degradation in the Zoige Basin,NE Tibetan Plateau using satellite remote sensing data

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Mountain Science 2017年第1期14卷 77-93页

作者： YU Kai-feng Frank LEHMKUHL Dimitri FALK Department of Geography RWTH Aachen University Templergraben 55 52056 Aachen Germany

Considerable efforts have been dedicated to desertification research in the arid and semi-arid drylands of central Asia. However,there are few quantitative studies in conjunction with proper qualitative evaluation concerning land degradation and aeolian activity in the alpine realm. In this study,spectral information from two Landsat-5 TM scenes(04.08.1994 and 28.07.2009,respectively) was combined with reference information obtained in the field to run supervised classifications of eight landscape types for both time steps. Subsequently,the temporal and spatial patterns of the alpine wetlands/grasslands evolutions in the Zoige Basin were quantified and assessed based on these two classification maps. The most conspicuous change is the sharp increase of ~627 km^2 degraded meadow. Concerning other land-covers,shallow wetland increases ~107 km^2 and aeolian sediments(mobile dunes and sand sheets) have an increase of ~30 km^2. Considering the deterioration,an obvious decrease of ~440 km^2 degraded wetland can be observed. Likewise,decrease of deep wetland(~78 km^2),humid meadow(~80 km^2) and undisturbed meadow(~88 km^2) were determined. These entire evolution matrixes undoubtedly hint a deteriorating tendency of the Zoige Basin ecosystem,which is characterized by significantly declined proportion of intact wetlands,meadow,rangeland and a considerable increase ofdegraded meadow and larger areas of mobile dunes. In particular,not only temporal alteration of the landcover categories,the spatial and topographical characteristics of the land degradation also deserves more attention. In the alpine rangelands,the higher terraces of the river channels along with their slopes are more liable to the degradation and desertification. This tendency has significantly impeded the nomadic and agriculture activities. The set of anthropozoogenic factors encompassing enclosures,overgrazing and trampling,rodent damaging and exceedingly ditching in the wetlands are assumed to be the main co

关键词： Alpine ecosystem Wetlands Degradation Desertification Zoige Basin Driving mechanisms

Amorphous Polylactide Bead Foam–Effect of Talc and Chain Extension on Foaming Behavior and Compression Properties

在线全文

学校读者我要写书评

暂无评论

Journal of Renewable Materials 2021年第11期9卷 1859-1868页

作者： Christian Brütting Julia Dreier Christian Bonten Volker Altstädt Holger Ruckdäschel Department of Polymer Engineering University BayreuthBayreuth95447Germany Institut für Kunststofftechnik University of StuttgartStuttgart70569Germany

Polylactide(PLA)bead foams show a high potential regarding their applicability in packaging or consumer *** the comparable properties of PLA to Polystyrene(PS)and the good CO_(2) footprint it represents a potential alternative to petroleum-based polymer ***,foaming of PLA is challenging,due to its low melt strength,therefore chemical modifiers are often *** the bead foam technology regarding PLA,the available literature is limited so *** this study,the bead foaming behavior of neat and modified amorphous PLA was *** material was modified by talc and an epoxy-based chain *** compounds have been investigated regarding their sorption behavior in CO_(2) atmosphere and their foaming *** was conducted by using the batch foaming method based on a rapid temperature increase after saturation with CO_(2).In order to achieve welded bead foams,a one-step processing for foaming and welding has been ***,the compression properties of the PLA bead foams have been *** below 50 kg/m^(3) for single bead foams and 80 kg/m^(3) for molded foams were achieved,respectively.

关键词： Polylactide biofoam bead foam CO_(2) low density

Finite Element Simulations of Crack Propagation in Al_(2)O_(3)/6061Al Composites

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2014年第5期27卷 853-861页

作者： Galina Lasko Ulrich Weber Siegfried Schmauder Institut für Materialprüfung Werkstoffkunde und Festigkeitslehre(IMWF)Universitat Stuttgart

In this work, the local fracture initiation behaviour of an Al2O3/6061Al composite is studied numerically. The damage behaviour of the microstructure is evaluated in consideration of the path and the amount of damage as well as the stress-strain performance of the microstructure. The damage behaviour of the ductile matrix has been simulated using the damage parameter D. For the simulation of fracture of the ceramic particles, a normal stress criterion is applied. For the analysis of the damage behaviour of the transition zone between particulate and matrix, both damage models （D parameter and normal stress criteria） are applied in this region. Parameter studies of crack propagation prediction in the Al2O3/6061Al composite on the basis of an Element Elimination technique have been performed for two differently heat-treated variants resulting in different mechanical properties. In addition, residual stress effects on the damage behaviour are examined for various microstructural situations.

关键词： Metal matrix composites Simulation Crack propagation Finite element method Element elimination technique

Assessing stress conditions and impact velocities in fluidized bed opposed jet mills

在线全文

学校读者我要写书评

暂无评论

Particuology 2020年第6期18卷 12-22页

作者： Alexander Strobel Benedikt Koninger Stefan Romeis Florian Schott Karl-Ernst Wirth Wolfgang Peukert Institute of Particle Technology(LFG) Friedrich-Alexander-Universitat Erlangen-NiirnbergCauerstrae 491058 ErlangenGermany

Fluidized bed opposed jet mills are capable of meeting the continuously growing dema nd for contamination-free fine *** this type of jet mill,the solid material is entrained and accelerated by expanding gas jets that are focused onto a focal point in side a fluidized *** resulting particle collisions induce *** process is affected by the relative particle velocities and the number of particle-particle ***,both quantities are ***,to date,neither relative particle velocities nor collision frequencies in such units have been *** present work introduces an innovative method to assess the stressing conditions in jet mills *** this end,mixtures of glass and ductile metal microspheres were used,with the latter employed in small ***-particle collisions between the aluminum and glass spheres lead to the formation of dents on the *** size and number of these dents are associated with the individual collision velocities and overall collision *** correlation between dent size and collision velocity was obtained from finite element calculations based on empirical *** proposed approach was validated using particle image velocimetry during secondary gas injection into a fluidized bed *** this case the effect of the distance between two opposed nozzles was *** a lab-scaled fluidized bed opposed jet mill the effects of gas pressure and hold-up were *** particle velocities were found to be sign ificantly lower tha n the gas velocities,while the nu mber of contacts per particle was determined to be extremely high.

关键词： Fluidized bed opposed jet mill Single particle probes Finite element modeling Stress number distribution Relative velocity distribution

Opportunities for large language models and discourse in engineering design

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Energy and AI

Energy and AI 2024年第3期17卷 136-142页

作者： Jan Göpfert Jann M.Weinand Patrick Kuckertz Detlef Stolten Institute of Energy and Climate research–Techno-economic Systems Analysis(IEK-3) Forschungszentrum Jülich GmbHJülich52425Germany Chair for Fuel Cells RWTH Aachen UniversityFaculty of Mechanical EngineeringAachen52062Germany

In recent years, large language models have achieved breakthroughs on a wide range of benchmarks in natural language processing and continue to increase in performance. Recently, the advances of large language models have raised interest outside the natural language processing community and could have a large impact on daily life. In this paper, we pose the question: How will large language models and other foundation models shape the future product development process? We provide the reader with an overview of the subject by summarizing both recent advances in natural language processing and the use of information technology in the engineering design process. We argue that discourse should be regarded as the core of engineering design processes, and therefore should be represented in a digital artifact. On this basis, we describe how foundation models such as large language models could contribute to the design discourse by automating parts thereof that involve creativity and reasoning, and were previously reserved for humans. We describe how simulations, experiments, topology optimizations, and other process steps can be integrated into a machine-actionable, discourse-centric design process. As an example, we present a design discourse on the optimization of wind turbine blades. Finally, we outline the future research that will be necessary for the implementation of the conceptualized framework.

关键词： Product development process Conceptual design Design methodology Design generation Natural language processing foundation models Multi-modal models