Eliminating viscosity bias in lateral flow tests

作     者：Daniel M.Kainz Bastian J.Breiner Susanna M.Fruh Tobias Hutzenlaub Roland Zengerle Nils Paust 

作者机构：Laboratory for MEMS ApplicationsIMTEK-Department of Microsystems EngineeringUniversity of FreiburgGeorges-Koehler-Allee 10379110 FreiburgGermany Hahn-SchickardGeorges-Koehler-Allee 10379110 FreiburgGermany 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2021年第7卷第5期

页      面：195-204页

核心收录：

学科分类：080704[工学-流体机械及工程] 080103[工学-流体力学] 08[工学] 0807[工学-动力工程及工程热物理] 0801[工学-力学（可授工学、理学学位）] 

基　　金：the German Research Foundation within the project CentriPhase(No.PA 2807/1-1) from the German Federation of Industrial Research Associations AIF under contract no.20436 N(project CentrlmA)is gratefully acknowledged 

主　　题：viscosity flow eliminate 

摘      要：Despite the widespread application of point-of-care lateral flow tests,the viscosity dependence of these assay results remains a significant ***,we employ centrifugal microfluidic flow control through the nitrocellulose membrane of the strip to eliminate the viscosity *** key feature is the balancing of the sample flow into the cassette of the lateral flow test with the air flow out of the cassette.A viscosity-indepen3ent flow rate of 3.01±0.18 min(±6%)is demonstrated for samples with viscosities ranging from 1.1 mPas to 24 mPas,a factor greater than *** a model human IgG lateral flow assay,signal-intensity shifts caused by varying the sample viscosity from 1.1 mPas to 23 mPas could be reduced by more than 84%.