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Methodologies for preparation of prokaryotic extracts for cell-free expression systems

Synthetic and Systems Biotechnology 2020年第4期5卷 252-267页

作者： Stephanie D.Cole Aleksandr E.Miklos Abel C.Chiao Zachary Z.Sun Matthew W.Lux US Army Combat Capabilities Development Command Chemical Biological Center 8567 Ricketts Point RoadAberdeen Proving GroundMD21010USA Division of Biology and Biological Engineering California Institute of TechnologyPasadenaCAUSA Synvitrobio Inc. San FranciscoCAUSA 8567 Ricketts Point Road Bldg E3549 Room B140APGMD21010USA

Cell-free systems that mimic essential cell functions,such as gene expression,have dramatically expanded in recent years,both in terms of applications and widespread *** we provide a review of cell-extract methods,with a specific focus on prokaryotic ***,we describe the diversity of Escherichia coli genetic strains available and their corresponding *** then trace the history of cell-extract methodology over the past 20 years,showing key improvements that lower the entry level for new ***,we survey the rise of new prokaryotic cell-free systems,with associated methods,and the opportunities ***,we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.

关键词： Cell-free systems Cell-free expression Cell-free extract Synthetic biology Methods

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Laser synthesis and functionalization of nanostructures

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International Journal of Extreme Manufacturing 2019年第1期1卷 38-50页

作者： Costas P Grigoropoulos Laser Thermal Laboratory Department of Mechanical EngineeringUniversity of California at Berkeley6129 Etcheverry HallBerkeley CA 94720-1740United States of America

This article summarizes work at the Laser Thermal Laboratory and discusses related studies on the laser synthesis and functionalization of semiconductor nanostructures and two-dimensional(2D)semiconductor *** has been carried out on the laser-induced crystallization of thin films and *** in situ transmission electron microscopy(TEM)monitoring of the crystallization of amorphous precursors in nanodomains is discussed *** directed assembly of silicon nanoparticles and the modulation of their optical properties by phase switching is *** vapor-liquid-solid mechanism has been adopted as a bottom-up approach in the synthesis of semiconducting nanowires(NWs).In contrast to furnace heating methods,laser irradiation offers high spatial selectivity and precise control of the heating mechanism in the time *** attributes enabled the investigation of NW nucleation and the early stage of nanostructure ***-and shape-selective,on-demand direct integration of oriented NWs was *** of discrete silicon NWs with nanoscale location selectivity by employing near-field laser illumination is also reported *** the properties of 2D transition metal dichalcogenides(TMDCs)by modulating the free carrier type,density,and composition can offer an exciting new pathway to various practical nanoscale *** situ Raman probing of laser-induced processing of TMDC flakes was conducted in a TEM instrument.

关键词： laser nanofabrication laser crystallization nanowire growth near field scanning optical microscopy transition metal dichalcogenides

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