Methodologies for preparation of prokaryotic extracts for cell-free expression systems

作     者：Stephanie D.Cole Aleksandr E.Miklos Abel C.Chiao Zachary Z.Sun Matthew W.Lux 

作者机构：US Army Combat Capabilities Development Command Chemical Biological Center8567 Ricketts Point RoadAberdeen Proving GroundMD21010USA Division of Biology and Biological EngineeringCalifornia Institute of TechnologyPasadenaCAUSA Synvitrobio Inc.San FranciscoCAUSA 8567 Ricketts Point RoadBldg E3549 Room B140APGMD21010USA 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2020年第5卷第4期

页      面：252-267页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：DARPA SBIR, (W911NF-16-P-0003) US Office of the Secretary of Defense Applied Research 

主　　题：Cell-free systems Cell-free expression Cell-free extract Synthetic biology Methods 

摘      要：Cell-free systems that mimic essential cell functions,such as gene expression,have dramatically expanded in recent years,both in terms of applications and widespread *** we provide a review of cell-extract methods,with a specific focus on prokaryotic ***,we describe the diversity of Escherichia coli genetic strains available and their corresponding *** then trace the history of cell-extract methodology over the past 20 years,showing key improvements that lower the entry level for new ***,we survey the rise of new prokaryotic cell-free systems,with associated methods,and the opportunities ***,we use this historical perspective to comment on the role of methodology improvements and highlight where further improvements may be possible.