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Ornithine decarboxylase gene is overexpressed in colorectal carcino.a

World Journal of Gastroenterology 2005年第15期11卷 2244-2248页

作者： Hai-YanHu Xian-XiLiu Chun-YingJiang YiLu Shi-LianLiu Ji-FengBian Xiao-MingWang ZhaoGeng YanZhang BingZhang ExperimentalCenterofMedicalMolecularBiology BiochemistryandMolecularBiologyInstituteSchoolofMedicineShandongUniversityJinan250012ShandongProvinceChina DepartmentofColo-proctology TheAffiliatedHospitalofShandongUniversityofTraditionChineseMedicineJinan250012ShandongProvinceChina

AIM: To investigate the ornithine decarboxylase (ODC)gene expression in colorectal carcino.a, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a mono.lonal antibody against fusion of human colon ODC prepared by hybridoma ***: Total RNA was extracted from human colorectal cancer tissues and their no.mal counterpart tissues. ODC mRNA levels were examined by *** genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immuno.istochemical and Western blotting ***: A cell line, which could steadily secrete antiODC mAb, was selected through subcloning four *** blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P＜0.01). Immuno.istochemical staining showed that colorectal carcino.a cells expressed a significantly higher level of ODC than no.mal colorectal mucosa (98.6±1.03%vs 5.26±5%, P＜0.01).CONCLUSION: ODC gene overexpression is significantly related to human colorectal carcino.a. ODC gene expression may be a marker for the gene diagno.is and therapy of colorectal carcino.a.

关键词： Ornithine decarboxylase RT-PCR Colorectal Cancer

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