Ornithine decarboxylase gene is overexpressed in colorectal carcinoma
Ornithine decarboxylase gene is overexpressed in colorectal carcinoma作者机构：ExperimentalCenterofMedicalMolecularBiologyBiochemistryandMolecularBiologyInstituteSchoolofMedicineShandongUniversityJinan250012ShandongProvinceChina DepartmentofColo-proctologyTheAffiliatedHospitalofShandongUniversityofTraditionChineseMedicineJinan250012ShandongProvinceChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2005年第11卷第15期
页 面：2244-2248页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by the Scientific Research Fund of National Ministry of Health No. 98-1-173
主 题：Ornithine decarboxylase RT-PCR Colorectal Cancer
摘 要：AIM: To investigate the ornithine decarboxylase (ODC)gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma ***: Total RNA was extracted from human colorectal cancer tissues and their normal counterpart tissues. ODC mRNA levels were examined by *** genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immunohistochemical and Western blotting ***: A cell line, which could steadily secrete antiODC mAb, was selected through subcloning four *** blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P0.01). Immunohistochemical staining showed that colorectal carcinoma cells expressed a significantly higher level of ODC than normal colorectal mucosa (98.6±1.03%vs 5.26±5%, P0.01).CONCLUSION: ODC gene overexpression is significantly related to human colorectal carcinoma. ODC gene expression may be a marker for the gene diagnosis and therapy of colorectal carcinoma.
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