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DNA barcoding provides distinction between Radix Astragali and its adulterants

Science china(Life sciences) 2010年第8期53卷 992-999页

作者： GUO HaiYan1,2. WANG WeiWei2. YANG Ning2. GUO BaoLin3, ZHANG Sun2. YANG RuiJing2. YUAN Ye2. YU JunLin4, HU SongNian2. SUN QiShi1 & YU Jun2.1School of Traditional chinese materia {2., Shenyang Pharmaceutical University, Shenyang 110016, china 2.ey Laboratory of Genome sciences and Information, Beijing institute of Genomics, chinese academy of sciences, Beijing 101300, china 3chinese academy of {2.l sciences, Peking Union {2.l College institute of Medicinal Plant Development, Beijing 100094, china 4Department of Chemistry, Tonghua Normal College, Tonghua 134002. china 1. School of Traditional chinese materia {2. Shenyang Pharmaceutical University Shenyang 110016 China2. Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing 101300 China3. Peking Union Medical College Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Beijing 100094 China4. Department of Chemistry Tonghua Normal College Tonghua 134002 China

Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including china, Japan, and Korea. However, in addition to the two species recorded in the chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2.parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional chinese herbs.

关键词： Radix Astragali DNA barcode Astragalus adulterant identification

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SOLVENT PARTICIPATION OF WITTIG OLEFINATION REACTION IN METHANOL

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chinese Chemical Letters 1992年第6期3卷 421-422页

作者： Shi Zhi CHEN Chang Jiu ZHANG Liang HUANG 1. institute of materia {2., chinese academy of {2.l sciences, Beijing 100050. 2. china Doping Control Center, Beijing 10002..

Wittig reaction in methanol was investigated, and a possible mechanism was proposed.

关键词： CCH SOLVENT PARTICIPATION OF WITTIG OLEFINATION REACTION IN METHANOL OCH Chen

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Inhibition of ATP-regulated potassium channels by agmatine in RINm5F insulin secreting cells

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中国药理学与毒理学杂志 1998年第2期12卷 70-71页

作者： LIANG　RiXin(institute of chinese {2. medica, china academy of Traditional chinese Medicine, Beijing 100700) 中国中医研究院中药所北京

ＩｎｈｉｂｉｔｉｏｎｏｆＡＴＰ┐ｒｅｇｕｌａｔｅｄｐｏｔａｓｉｕｍｃｈａｎｎｅｌｓｂｙａｇｍａｔｉｎｅｉｎＲＩＮｍ５ＦｉｎｓｕｌｉｎｓｅｃｒｅｔｉｎｇｃｅｌｓＬＩＡＮＧＲｉ－Ｘｉｎ（ＩｎｓｔｉｔｕｔｅｏｆＣｈｉｎｅｓ... 详细信息

ＩｎｈｉｂｉｔｉｏｎｏｆＡＴＰ┐ｒｅｇｕｌａｔｅｄｐｏｔａｓｉｕｍｃｈａｎｎｅｌｓｂｙａｇｍａｔｉｎｅｉｎＲＩＮｍ５ＦｉｎｓｕｌｉｎｓｅｃｒｅｔｉｎｇｃｅｌｓＬＩＡＮＧＲｉ－Ｘｉｎ（ＩｎｓｔｉｔｕｔｅｏｆＣｈｉｎｅｓｅＭａｔｅｒｉａＭｅｄｉｃａ，Ｃｈ...

关键词： patchclamp technique agmatine ion channels, potassium

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SYNTHESIS OF PEG-DENDRIMERIC PEPTIDE

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chinese Chemical Letters 1996年第8期7卷 693-694页

作者： De Xin WANG and Lei HUANG(institute of materia {2., chinese academy of {2.l sciences and Peking Union {2.l College,Beijing 100050) chinese ACAD MED SCI INST MAT MEDBEIJING 100050PEOPLES R CHINA BEIJING UNION MED COLL BEIJING 100050PEOPLES R CHINA

Synthesis of a new macromolecule with MW up to 7.6 KDA is described in the present paper. The ligation between a dendrimeric amino-PEG and unprotected peptides via a site- specific reaction is a useful trial for the p... 详细信息

关键词： PEG SYNTHESIS OF PEG-DENDRIMERIC PEPTIDE

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Studies on the Preparation of Bioactive Oligomerstilbene by Oxidative Coupling Reaction(Ⅰ)-Preparation of Shegansu B using Silver Oxide as Oxidant

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chinese Chemical Letters 2000年第6期11卷 515-516页

作者： Li Xin ZHOU Mao LIN(institute of materia {2.，chinese academy of {2.l sciences，Peking Union {2.l College, Beijing 100050) institute of materia {2. Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100050 China

篢he oxidative coupling reaction of isorhapontigenin using sliver oxide as oxidant afforded a major product, named shegansu B（2.， which was isolated from the roots of Belamcanda chinensis (L．)DC. Both the natural ... 详细信息

关键词： Isorhapontigenin oligomerstilbene shegansu B oxidative coupling reaction silver oxide

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An Efficient Cleavage of 1,3-Dioxolane Using ZnCl_2.THF/H_2.

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chinese Chemical Letters 2000年第2期11卷 95-96页

作者： Li Gong OU Dong Lu BAI (Shanghai institute of materia {2., the chinese academy of sciences 2.4 Taiyuan Road, Shanghai, 2.0031) chinese Acad Sci Shanghai Inst Mat Med Shanghai 200031 Peoples R China

A new. mild and efficient method for the cleavage of 1,3-dioxolane is reported.

关键词： 1,3-Dioxolane ketal deprotection

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Lactariolide,a New 14-Membered-Ring Compound from Lactarius Subvellereus

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chinese Chemical Letters 1997年第2期8卷 135-136页

作者： Jing ZHANG Xiao Zhang FENG(institute of materia {2., chinese academy of {2.l sciences and Peking Union {2.l College, Beijing, 100050) chinese ACAD MED SCI INST MAT MEDBEIJING 100050PEOPLES R CHINA BEIJING UNION MED COLL BEIJING 100050PEOPLES R CHINA

Lactariolide (I), a new 14-membered-ring lactone was isolated from the fruit bodies of Lactarius subvellereus (Russulaceae), and its structure was elucidated as (R)-13-hydroxy-trans-9, 11-octadecadienoic acid lactone ... 详细信息

关键词： Cl Lactariolide,a New 14-Membered-Ring Compound from Lactarius Subvellereus

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The Synthesis of Aromatic Hydrazones as Nitrogen-Containing Prostaglandin Congeners

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chinese Chemical Letters 1998年第6期9卷 529-531页

作者： Guo Hao XIE Yan Mei HE Yuan Liu WU(institute of materia {2., chinese academy of {2.l sciences, Beijing 100050) chinese Acad Med Sci Inst Mat Med Beijing 100050 Peoples R China

We synthesized a series of hydrazones, a novel type of nitrogen-containing prostaglandins, from different aromatic hydrazines and variant 2.substituted amino-3-hydroxy bicycle [3.2.0]hept-6-ones IV.

关键词： aromatic hydrazine hydrazone nitrogen-containing prostaglandin

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Conformational flexibility of β-secretase: molecular dynamics simulation and essential dynamics analysis

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Acta Pharmacologica Sinica 2004年第6期25卷 3-11页

作者： Bing XIONG, Xiao-qin HUANG, Ling-ling SHEN, Jian-hua SHEN 2.Xiao-min LUO, Xu SHEN, Hua-liang JIANG2. Kai-xian CHENDrug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai institute of materia {2.,Shanghai institutes for Biological sciences, chinese academy of sciences, Shanghai 2.12.3, china Drug Discovery and Design Center State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

AIM: Based on the structural analysis to reveal the mechanism of ligand binding to b-secretase and the specificityof each binding sub-site. METHODS: Molecular dynamics was used to simulate on the ligand free b-secretase andligand bound b-secretase. The trajectories were analyzed using the essential dynamics, and the significant confor-mational change was illustrated employing the DynDom program. RESULTS: The essential dynamics and DynDomanalyses clearly showed that the b-secretase experienced a large conformational change upon the substrate orinhibitor binding. The flap structure adopted a swing motion, gradually covering the active site to facilitate theligand binding process. Residues Ser86 and Ile87 served as the hinge point. Inhibitor-enzyme interaction analysisrevealed that residues at P2. P1, and P1 positions of the inhibitor were very important for the binding, and residuesat P2.and P3 positions may be modified to improve the binding specificity. S3 subsite of the enzyme still hadspace to modify the inhibitors in increasing the binding affinity. CONCLUSION: The information presented hereis valuable and could be used to identify small molecular inhibitors of b-secretase.

关键词： molecular dynamics secretase essential dynamics computer simulation Alzheimer disease

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A novel synthetic use of 2.bromo- 1-methylpyridinium iodide for macrolactamization

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chinese Journal of Chemistry 1994年第2期12卷 158-168页

作者： BAI, Dong-Lu BO, Yun-Xin ZHOU, Qi-Ting Shanghai institute of materia {2., chinese academy of sciences, Shanghai 2.0031, china Shanghai institute of Materia Medica Chinese Academy of Sciences Shanghai 200031 China

Using 2.bromo-1-methylpyridimium iodide as carboxyl activating agent, 10 ansa-macrolactams were prepared conveniently from the corresponding seco-precursor w-aminoacids in the yields of 78-2..

关键词： Lactam ansa-macrolactam cyclization lactamization.

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