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The PI3K-Akt Pathway Inhibits senescence and Promotes Self-Renewal of Human Skin-Derived Precursors in Vitro

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The PI3K-Akt Pathway Inhibits Senescence and Promotes Self-R...

甘草叶中黄酮成分体外诱生细胞毒因子的实验研究

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Shuang Liu,~(a,b,1) Shu Liu,~(a,b1) Xinyue Wang,~(a,b)Jiaxi Zhou,~c Yujing Cao,~a Fei Wang,~d Enkui Duan~(a2) a.State Key laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing,People’s Republic of china b.Graduate university of the Chinese Academy of Sciences,Beijing,People’s Republic of china c.State Key laboratory of Experimental Hematology,Institute of Hematology & Blood Diseases Hospital,Chinese Academy of medical Sciences & Peking Union medical college d.Department of cell and Developmental Biology and Institute for Genomic Biology,university of Illinois at Urbana-Champaign,Urbana, IL,USA

Skin-derived precursors(SKPs) are embryonic neural crest or somite-derived multipotent progenitor cells with properties of dermal stem *** a large number of studies deal with their differentiation ability and potential applications in tissue damage repair,few studies have concentrated on the regulation of SKP ***,we found that after separation from their physiological microenvironment,human foreskin-derived SKPs(hSKPs) quickly senesced and lost their self-renewal *** observed a sharp decrease in Akt activity during this process,suggesting a possible role of the PI3K-Akt pathway in hSKP maintenance in *** this pathway with several inhibitors inhibited hSKP proliferation and sphere formation,and increased hSKP *** contrast,activating this pathway with PDGF-AA and a PTEN inhibitor,bpV(pic),promoted proliferation,improved sphere formation,and alleviated senescence of hSKPs,without altering their differentiation *** also implied that this effect was associated with altered actions of FoxO3 and GSK-3β.Our results suggest an important role of the PI3K-Akt pathway in the senescence and self-renewal of human foreskin-derived *** findings also provide a better understanding of the cellular mechanisms underlying hSKP self-renewal and stem cell senescence to allow more efficient expansion of hSKPs for regenerative medical applications.

关键词： skin-derived precursors PI3K-Akt self-renewal cellular senescence adult stem cells PTEN

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汕头大学医学院学报 1994年第2期7卷 9-11页

作者：董菁王秀梅杨棉华余秀葵汕头大学医学院微生物室内蒙古医学院微生物室

本实验对甘草叶中的黄酮成分（总黄酮）在体外诱导小鼠骨髓巨噬细胞（ＢＭＣ）及腹腔巨噬细胞（ＰＥＣ）产生细胞毒因子的作用进行了研究，通过体外细胞毒试验及对黄酮成分诱生因子的活性测定和中和试验证明：在一定浓度下，甘草叶中的... 详细信息

本实验对甘草叶中的黄酮成分（总黄酮）在体外诱导小鼠骨髓巨噬细胞（ＢＭＣ）及腹腔巨噬细胞（ＰＥＣ）产生细胞毒因子的作用进行了研究，通过体外细胞毒试验及对黄酮成分诱生因子的活性测定和中和试验证明：在一定浓度下，甘草叶中的黄酮类成分对ＢＭＣ和ＰＥＣ及Ｌ９２９细胞无直接毒性作用但能诱导ＢＭＣ和ＰＥＣ产生具有杀伤作用的细胞毒因子。经中和试验初步证明此细胞毒因子为肿瘤坏死因子（ＴＮＦａ）。

关键词：抗肿瘤作用中药药理学

抑癌基因PTEN对人结肠癌LoVo细胞生长的影响

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吉林医学 2004年第8期25卷 15-18页

作者：徐寿水沈文律欧阳松应汕头大学医学院第二附属医院广东汕头515031 中国人民解放军军需大学军事兽医系吉林长春130061

目的:探讨PTEN基因转染对结肠癌LoVo细胞的抑制作用。方法:将人野生型及突变型PTENcDNA通过脂质体导入结肠癌细胞LoVo中,并用RT-PCR、流式细胞术检测细胞的PTENmRNA和蛋白质表达。用流式细胞仪和MTT法分析5-Fu对转染前后LoVo细胞抑制周... 详细信息

目的:探讨PTEN基因转染对结肠癌LoVo细胞的抑制作用。方法:将人野生型及突变型PTENcDNA通过脂质体导入结肠癌细胞LoVo中,并用RT-PCR、流式细胞术检测细胞的PTENmRNA和蛋白质表达。用流式细胞仪和MTT法分析5-Fu对转染前后LoVo细胞抑制周期和诱导凋亡的能力。结果:转染野生型PTEN基因可明显增强结肠癌LoVo细胞的PTEN表达,5-Fu对转染后的LoVo细胞抑制细胞周期和诱导凋亡的作用明显增强。突变型在结肠癌细胞LoVo中有表达但无明显抑制作用。结论:转染的PTEN基因可显著上调结肠癌LoVo细胞的PTEN表达,促进细胞凋亡;联合应用PTEN基因转染和5-Fu可获得协同的细胞毒作用。

关键词：抑癌基因 PTEN基因结肠癌 LoVo细胞生长肿瘤抑制作用

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造血干细胞移植病人营养支持的研究

护理研究 2004年第8期18卷 682-684页

作者：应文娟陈惜遂卢锡芝刘元生苏永忠蔡应木汕头大学医学院第一附属医院

[目的 ]探讨肠外营养支持对白血病病人造血干细胞移植期间的营养状态的影响。 [方法 ]分析 12例白血病病人在造血干细胞移植期间接受胃肠外营养支持治疗后临床症状、体重、血清白蛋白、血糖、血尿素氮及肌酐等指标的变化 ,并与同期未行... 详细信息

[目的 ]探讨肠外营养支持对白血病病人造血干细胞移植期间的营养状态的影响。 [方法 ]分析 12例白血病病人在造血干细胞移植期间接受胃肠外营养支持治疗后临床症状、体重、血清白蛋白、血糖、血尿素氮及肌酐等指标的变化 ,并与同期未行胃肠外营养支持治疗的 10例病人相关指标进行比较。[结果 ]接受胃肠外营养支持组 (PN组 )病人移植期间体重下降较对照组病人少 ,血清白蛋白水平则较对照组高。 [结论 ]胃肠外营养支持对造血干细胞移植病人平稳度过预处理期、放疗、化疗后应激反应期与促进造血功能的恢复有十分重要的意义

关键词：白血病造血干细胞移植胃肠外营养

A NEW ANIMAL MODEL OF ERYTHROCYTE senescence: THE ALTERRATION OF SURFACE CHARGE AND THE RHEOLOGGICAL PROPERTIES OF ERRYTHROCYTE DRUING ITS LIFE SPAN

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Chinese Journal of Biomedical Engineering(English Edition) 1998年第2期7卷 75-86页

作者： Wen Zongyao Song Lichua Lu Zhihong Sun Dagong(Department of medical Physics, Beijing medical university, Beijing 100083,china) Yan Zongyi(Department of Mechanics and Engineering SciencePeking university,Beijing 100871,china) Don Huanfu(Department of H

Based on Mohandas＇ work, a new animal model of red blood cell(RBC) synchronous senescence in a near normal physiological conditions was proposed. Using this model,we studied the changes of RBC surface charge with the aging process. The results showed that its surface charge decreased with RBC agingprocess. Nevertheless,the regulation of the changes was not linear during RBC aging. The indexes of (DI)d and (DI)or describing RBC rheological behaviours duringits aging process were measured with a new Ektacytometry. It is demonstrated thatboth the indexes decreased with RBC aging,but the relationship was not linear. Thecharacteristics of the indexes displayed a common regularity of smaller alterationsin the first half period of RBC life span and greater alterations in the latter periodof RBC life span. It was also shown that the regulation of the changes for each individual index were not alike. The alterations of RBC mechanical properties duringlife span maight result from the influence of RBC shape, the properties of cytoplasm and membrane viscoelasity and the other factors during RBC aging.

关键词： erythrocyte senescence surface charge rheological properties

Identification and analysis of differentially expressed genes involved in dark-induced photoperiod response and senescence of soybean leaves by suppression subtractive hybridization

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湖南农业大学学报（自然科学版） 2007年第S1期33卷 222-页

作者： ZHAO Lin1,LUO Qiu-lan1,HAN Ying-peng1,YANG Chun-liang2,LI Wen-bin1(1.Soybean Research Institute(Key laboratory of Soybean Biology of Chinese Education Ministry),Northeast Agriculture university,Haerbin,china 150030 2.The Basic medicalScience college,Harbin medical university,Haerbin,china 150081)

A cDNA subtractive library enriched for dark-induced up-regulated ESTs was constructed by suppression subtractive hybridization(SSH) from leaf tissues of soybean cultivar DongNong L13 treated with short-day(8-h light/16-h dark) and long-day(16-h light/8-h dark) conditions.A total of 148 clones were sequenced,representing 76 unique ESTs which corresponded to about 20% of 738 clones from the cDNA library and showed a significant up-regulation of at least three fold verified by dot blot *** putative functions of ESTs were predicted by Blastn and *** 43 differentially expressed genes identified by subtractions were classified according to their putative functions generated by Blast *** functional analysis indicated that putative proteins encoded by these genes were related to diverse functions during organism development,which include biological regulation pathways such as transcription,signal transduction and programmed cell death,protein,nucleic acid and carbohydrate macromolecule degradation,the cell wall modification,primary and secondary metabolism and stress *** soybean transcription factors enhanced in SD conditions,GAMYB-binding protein and DNA binding protein RAV cDNAs that may be involved in SD soybean photoperiod response,had been isolated using 5'-and 3'-rapid amplification of cDNA ends(RACE)(Genbank Accession numbers DQ112540 and DQ147914).

关键词： cDNA Identification and analysis of differentially expressed genes involved in dark-induced photoperiod response and senescence of soybean leaves by suppression subtractive hybridization ESTs

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食管癌p53基因突变的研究

汕头大学医学院学报 1997年第1期10卷 1-4页

作者：邵根泽张少英任运生汕头大学医学院肿瘤分子生物学研究室

应用非同位素PCR－SSCP方法分析30例食管瘤患者癌组织及相应癌旁组织p53基因第5～9外显子，结果发现8例癌组织存在p53基因突变；其中的4例为p53基因杂合性丢失（LOH）。部分癌旁组织亦检测到p53基因突变。实验结果表明，p53基因突变可... 详细信息

应用非同位素PCR－SSCP方法分析30例食管瘤患者癌组织及相应癌旁组织p53基因第5～9外显子，结果发现8例癌组织存在p53基因突变；其中的4例为p53基因杂合性丢失（LOH）。部分癌旁组织亦检测到p53基因突变。实验结果表明，p53基因突变可能是食管癌癌变的早期遗传学损害，对食管癌的发生起重要作用。

关键词： P53基因基因突变食管肿瘤

缺血损伤诱导大鼠腹主动脉移植硬化的组织病理学研究

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汕头大学医学院学报 1999年第3期12卷 6-8页

作者：万云乐沈文律罗义刚李幼平李波汕头大学医学院第二附属医院普外科汕头515041 加拿大西安太略大学医院华西医科大学第一临床医学院普外科成都610041

目的 :研究缺血损伤诱导的同品系大鼠腹主动脉移植硬化的组织病理学改变 ,并探讨其可能机理。方法 :1 4 0只大鼠随机分组 :A组为非移植组 ,B组为同品系移植缺血 <3 0min组 ;C组为同品系移植缺血 4h组 ;D组为异品系移植缺血 <3 0min组。... 详细信息

目的 :研究缺血损伤诱导的同品系大鼠腹主动脉移植硬化的组织病理学改变 ,并探讨其可能机理。方法 :1 4 0只大鼠随机分组 :A组为非移植组 ,B组为同品系移植缺血 3 0min组 ;C组为同品系移植缺血 4h组 ;D组为异品系移植缺血 3 0min组。术后 7天、 1 5天、 3 0天、 60天切取植入的腹主动脉进行光、电镜检查。结果 :移植早期C组动脉内膜有多量多形核白细胞和单个核细胞粘附、浸润 ,电镜检查多形核白细胞为中性白细胞。而D组移植动脉各层均有大量的淋巴细胞浸润。移植后期C组、D组移植动脉内膜均有显著增厚 ,增生的内膜均由单核 /巨噬细胞和平滑肌细胞构成 ,但前者无中膜层平滑肌坏死及弹力膜断裂现象。结论 :中性白细胞在缺血 /再灌注损伤诱导的同品系大鼠腹主动脉移植硬化中发挥了重要作用。

关键词：缺血再灌注损伤移植动脉硬化组织病理学

影响造血干细胞移植患者生活质量的相关因素分析

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实用全科医学 2008年第1期6卷 10-11页

作者：苏永忠陈惜遂李映华刘元生广东省汕头大学医学院第一附属医院血液内科 515041

目的探讨造血干细胞移植患者生活质量现状及相关影响因素。方法参考我国癌症患者化学生物治疗生活质量表(QOL-CCC),制定一份一般情况调查表,对25例进行造血干细胞移植的患者在移植后进行问卷调查并分析。结果造血干细胞移植患者的QOL评... 详细信息

目的探讨造血干细胞移植患者生活质量现状及相关影响因素。方法参考我国癌症患者化学生物治疗生活质量表(QOL-CCC),制定一份一般情况调查表,对25例进行造血干细胞移植的患者在移植后进行问卷调查并分析。结果造血干细胞移植患者的QOL评分基本满意。躯体维度对生活质量影响最大,受移植方式、性别、家人关怀、文化程度、家庭收入的影响。结论应采取综合的、有计划的、全方位的和个性化的措施具体对待,减轻患者躯体病痛,满足必需的精神需求,以提高造血干细胞移植后患者的生活质量。

关键词：造血干细胞移植生活质量影响因素