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In vitro derivation of functional insulin-producing cells from human embryonic stem cells

Cell Research 2007年第4期17卷 333-344页

作者： Wei Jiang~(1,*) Yan Shi~(1,5,*) Dongxin Zhao~1 Song Chen~1 Jun Yong~1 Jing Zhang~2 Tingting Qing~1 Xiaoning Sun~(1,3) Peng Zhang~1 Mingxiao Ding~1 Dongsheng Li~4 Hongkui Deng~(1,2,3) ~1Department of Cell Biology and Genetics,College of Life Sciences,peking university,Beijing 100871,china ~2Beijing laboratory Animals Research Center,Beijing 100012,china ~3laboratory of chemical genomics,shenzhen graduate school of peking universit,the university town,shenzhen 518055,china ~4provincial Kev laboratory of Embryonic Stem Cell Research,Tai-He Hospital Yunyang Medical College,32 S.Renmin Rd.,Shiyan 442000,china Department of Cell Biology and Genetics College of Life Sciences Peking University Beijing China 5Present address: The Scripps Research Institute Chemistry Department SP3130 10550 North Torrey Pines Road La Jolla CA USA. Beijing laboratory Animals Research Center Beijing China laboratory of chemical genomics Shenzhen Graduate School of Peking University the University Town Shenzhen China Provincial Key laboratory of Embryonic Stem Cell Research Tai-He Hospital Yunyang Medical College Shiyan China

The capacity for self-renewal and differentiation of human embryonic stem(ES)cells makes them a potential source forgeneration of pancreatic beta cells for treating type I diabetes ***,we report a newly developed and effectivemethod,carried out in a serum-free system,which induced human ES cells to differentiate into insulin-producing *** A was used in the initial stage to induce definitive endoderm differentiation from human ES cells,as detectedby the expression of the definitive endoderm markers Sox 17 and ***,all-trans retinoic acid(RA)wasused to promote pancreatic differentiation,as indicated by the expression of the early pancreatic transcription factorspdr1 and *** maturation in DMEM/F12 serum-free medium with bFGF and nicotinamide,the differentiatedcells expressed islet specific markers such as C-peptide,insulin,glucagon and *** percentage of C-peptide-posi-tive cells exceeded 15%.The secretion of insulin and C-peptide by these cells corresponded to the variations in *** transplanted into renal capsules of Streptozotocin(STZ)-treated nude mice,these differentiated human EScells survived and maintained the expression of beta cell marker genes,including C-peptide,pdx1,glucokinase,nkx6.1,IAPP,pax6 and *** percent of the transplanted nude mice exhibited apparent restoration of stable euglycemia;and the corrected phenotype was sustained for more than six *** new method provides a promising in vitro dif-ferentiation model for studying the mechanisms of human pancreas development and illustrates the potential of usinghuman ES cells for the treatment of type I diabetes mellitus.

关键词： human embryonic stem cell direct differentiation insulin-producing cell diabetes

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Generation of pancreatic islet cells from human embryonic stem cells

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Science china(Life Sciences) 2009年第7期52卷 615-621页

作者： ZHANG DongHui1,2,JIANG Wei1,SHI Yan2 & DENG HongKui1,2 1 Key laboratory of Cell Proliferation and Differentiation of the Ministry of Education,College of Life Sciences,peking university,Beijing 100871,china 2 laboratory of chemical genomics,shenzhen graduate school of peking university,shenzhen 518055,china Key laboratory of Cell Proliferation and Differentiation of the Ministry of Education College of Life Sciences Peking University Beijing China laboratory of chemical genomics Shenzhen Graduate School of Peking University Shenzhen China

Efficiently obtaining functional pancreatic islet cells derived from human embryonic stem(hES) cells not only provides great potential to solve the shortage of islets sources for type I diabetes cell therapy,but also benefits the study of the development of the human pancreas and diabetes *** 2001,hES cells were reported to have the capacity to generate insulin-producing cells by spontaneous differentiation in *** then,many strategies(such as overexpression of key transcription factors,delivery of key proteins for pancreatic development,co-transplantation of differentiated hES cells along with fetal pancreas,stepwise differentiation by mimicking in vivo pancreatic development) have been employed in order to induce the differentiation of pancreatic islet cells from hES ***,patient-specific induced pluripotent stem(iPS) cells can be generated by reprogramming somatic *** cells have characteristics similar to those of ES cells and offer a new cell source for type I diabetes cell therapy that reduces the risk of immunologic *** this review,we summarize the recent progress made in the differentiation of hES and iPS cells into functional pancreatic islet cells and discuss the challenges for their future study.

关键词： human embryonic stem(hES) pancreatic islet cells differentiation induced pluripotent stem(iPS) cells

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Pollution characteristics of atmospheric fine particles and their secondary components in the atmosphere of shenzhen in summer and in winter

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Science china Chemistry 2006年第5期49卷 466-474页

作者： NIU Yuwen1,2,HE Lingyan1,2,HU Min2,ZHANG Jing2 & ZHAO Yunliang2 1. shenzhen graduate school of peking university,shenzhen 518055,china 2. State Joint Key laboratory of Environmental Simulation and Pollution Control,College of Environmental Sciences,peking university,Beijing 100871,china shenzhen graduate school of peking university Shenzhen China State Joint Key laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences Peking University Beijing China

Two field measurements for atmospheric fine particles were conducted in Baoan district of shenzhen during the summer and winter in 2004. Totally 30 sets of 24 h samples were collected, and then the mass concentrations and chemical compositions were determined. The seasonal varia- tions and secondary pollution characteristics of fine particles during the sampling periods were dis-cussed with meteorological factors. The results show that seasonal variations of atmospheric particles are significant in shenzhen. The average mass concentrations of PM2.5 and PM10 in summer were 35 μg·m-3 and 57 μg·m-3, respectively, and those in winter were 99 μg·m-3 and 135 μg·m-3, respec-tively. The concentrations of both PM2.5 and PM10 in winter increased 184% and 137%, respectively, compared to those in summer. PM2.5 accounted for 61% and 75% of PM10 in summer and in winter, respectively, indicating severe fine particle pollution in shenzhen. During the summer and winter sampling periods, the mean OC/EC ratios were 3.4 and 1.6, respectively. The estimated secondary organic carbon (SOC) averagely accounted for 56% and 6% of the total OC in summer and in winter, respectively, which implies a major contribution of SOC to OC in summer. During the continuous high temperature period in summer, both the concentrations and fractions of secondary aerosol compo-nents in PM2.5 were highly elevated, suggesting severe secondary pollution again. The prevailing wind was from South china Sea in summer, and the air quality was good. The prevailing wind in winter was from china's Mainland to the north, and the polluted air mass led to poor air quality.

关键词： PM2.5, mass concentration, secondary pollution, seasonal variation, shenzhen.

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Identification of secondary organic aerosols based on aerosol mass spectrometry

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中国科学：化学英文版 2010年第12期53卷 2593-2599页

作者： HUANG XiaoFeng 1,ZHAO QianBiao 1,2,HE LingYan 1,HU Min 2,BIAN QiJing 3,XUE Lian 1 & ZHANG YuanHang 2 1 Key laboratory for Urban Habitat Environmental Science and Technology school of Environment and Energy,peking university shenzhen graduate school,shenzhen 518055,china 2 State Key Joint laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering,peking university,Beijing 100871,china 3 Division of Environment,Hong Kong university of Science & Technology,Hong Kong,china Key laboratory for Urban Habitat Environmental Science and Technology school of Environment and Energy Peking University Shenzhen Graduate School Shenzhen China State Key Joint laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering Peking University Beijing China Division of Environment Hong Kong University of Science & Technology Hong Kong China

Secondary organic aerosol (SOA) is one of the major components of aerosols in the atmosphere and has not been well understood so *** to the complex chemical composition of organic aerosols,the identification of SOA has been a hotspot and difficult issue in the field of aerosol *** study attempts to quantitatively identify SOA in winter of shenzhen based on positive matrix factorization (PMF) *** sources were resolved and SOA was identified subsequently according to the characteristic ion fragments measured by highly time-resolved aerosol mass spectrometer *** showed that in the winter of shenzhen the average SOA concentration was 9.41±6.33 g/m 3,accounting for 39.9±21.8% of the total organic *** with primary organic aerosol (POA),the SOA concentrations had no large variation,suggestive of characteristics of regional secondary *** ratio of SOA/BC had pronounced diurnal variation,similar to that of O x (O3+NO2),indicating SOA formation was significantly controlled by activity of photochemistry in the *** most effective period for SOA formation was from 9 am～3 pm since the SOA/BC ratio increased by 122% during this *** study provides a new technical method and a new idea for SOA investigation.

关键词： secondary organic aerosol aerosol mass spectrometry positive matrix factorization (PMF)

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Zebrafish based chemical screening

Zebrafish based chemical screening

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2010·第一届斑马鱼学术研讨会

作者： Hanbing Zhong and Shuo Lin laboratory of chemical genomics,school of chemical Biology and Biotechnology,peking university,shenzhen graduate school,shenzhen,china,518055

Functional chemicals are very useful tools for biology *** to its small size,large progeny clutch,and embryonic transparency,zebrafish serves as a superb in vivo animal model for chemical compound screens and characte...

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High time resolution observation and statistical analysis of atmospheric light extinction properties and the chemical speciation of fine particulates

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Science china Chemistry 2010年第8期53卷 1801-1808页

作者： YAO TingTing1,HUANG XiaoFeng1,HE LingYan1,HU Min2,SUN TianLe1,XUE Lian1,LIN Yun1,ZENG LiWu1 & ZHANG YuanHang2 1Key laboratory for Urban Habitat Environmental Science and Technology,school of Environment and Energy,peking university shenzhen graduate school,shenzhen 518055,china 2State Key Joint laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering,peking university,Beijing 100871,china Key laboratory for Urban Habitat Environmental Science and Technology School of Environment and Energy Peking University Shenzhen Graduate School Shenzhen China State Key Joint laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering Peking University Beijing China

In recent years,the visibility deterioration caused by regional fine particulate pollution becomes one of the crucial air pollution problems in the urban areas of our *** rapid variation of visibility and fine particulates make it difficult to estimate the relationship between them precisely and accurately unless high time resolution observation data can be *** study aims to fill this gap in the field of atmospheric science by establishing a formula using multiple linear *** fitting goodness (R2=0.913,n=3167) was obtained using 10 min average of high-resolution real-time light scattering coefficients,light absorption coefficients,main chemical speciation concentration in PM1 and some meteorological parameters from 17 Jan to 16 Feb,*** shows that the average light extinction coefficient during the observation in the winter of shenzhen was measured to be 290 ± 183 Mm?1,consisting of 72% of light scattering and 21% of *** terms of the percentage contribution of PM1 chemical species to the total light extinction,the organic matter was estimated to be most with an average of 45%,followed by ammonium sulfate with an average of 24%.The contributions of black carbon and ammonium nitrate were 17% and 12%,***,the diurnal variation of light extinction was investigated as well in this study.

关键词： light extinction PM1 chemical speciation high time resolution multiple linear regressions

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Development of Novel Thiourea and Furancarbothioamide palladacycle Based Ligands in the Palladium-Catalyzed Reactions

Development of Novel Thiourea and Furancarbothioamide pallad...

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中国化学会第十三届金属有机化学学术讨论会

作者： Mingji Dai, Xiong, Zhengchang, Jiahua Chen, and Zhen Yang Key laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Beijing, 100871, china, laboratory of chemical Genetics, shenzhen graduate school of peking university, the university town, shenzhen, 518055, china

＜正＞ The Pd-catalyzed cross-coupling reaction is a method for C-C bond formation. In this context, the design of novel ligands and transition metal catalysts that can effect desired transformations with both high efficiency and high selectivity has been a fertile area of research. Phosphine-based ligands have played very important roles in the Pd-catalyzed cross-coupling reactions. However these ligands usually need to be handled in an inert atmosphere or in dry conditions, and sometimes suffer from P-C bond degradation at elevated temperatures, which leads to palladium aggregation and affects the catalytic activity.

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Application of Diversity Oriented Synthesis （DOS） to Identification of Biologically Active Molecules

Application of Diversity Oriented Synthesis （DOS） to Ident...

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中国化学会第26届学术年会化学生物分会场

作者： Zhen Yang laboratory of chemical genomics, shenzhen graduate school, Key laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education and Beijing National laboratory for Molecular Science (BNLMS), College of Chemistry, peking university, Beijing 100871

＜正＞Diversity-oriented synthesis （DOS） can be used to synthesize diversified molecules on a large scale. As with all large-scale experiments, this process requires a major investment in equipment, consumables and time. Therefore, careful careful design is critical. As the complexity of the libraries to be generated increases, additional considerations become important. What are the issues that should be considered when

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Zebrafish tyrosine hydroxylase 2 gene encodes tryptophan hydroxylase rather than tyrosine hydroxylase

Zebrafish tyrosine hydroxylase 2 gene encodes tryptophan hyd...

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2012全国发育生物学大会

作者： Guiqi Ren~1,Lu Chen~1,Song Li~1,Hanbing Zhong~1,Shuo Lin~(1,2) 1 laboratory of chemical genomics,school of chemical Biology and Biotechnology,peking university shenzhen graduate school,shenzhen,china 2 Department of Molecular,Cell and Developmental Biology,university of California Los Angeles,Los Angeles, California,United States of America

The primary cause of Parkinson’s disease(PD) is the profound loss of dopaminergic(DA) neurons in the substantia nigra pars *** facilitate the understanding of the underling mechanism of PD,several studies have focused on generating zebrafish PD models,which can recapitulate the characteristic of DA neuron *** zebrafish studies, tyrosine hydroxylase 1(th1) has been frequently used as a marker of DA neurons,although it also labels norepinephrine and epinephrine ***,a homologue of th1,named tyrosine hydroxylase 2(th2) was identified based on the sequence homology and subsequently used as a novel marker of DA *** this study,we present evidence that th2 co-localizes with serotonin in the ventral diencephalon and caudal hypothalamus in zebrafish *** addition, knock-down of th2 reduces the production of serotonin in the corresponding th2 positive *** phenotype can be rescued by both zebrafish th2 and mouse tryptophan hydroxylase(Tph1) mRNA,as well as by the product of tryptophan hydroxylase,***,the purified th2 protein has the tryptophan hydroxylase activity comparable with the mouse Tph1 protein in *** on our in vivo and in vitro studies,we conclude that th2 is a gene coding for tryptophan hydroxylase and should be used as a marker gene of serotonergic neurons. Conclusions:Our data support the idea that th2 is a gene coding for tryptophan hydroxylase and should be used as a marker gene of serotonergic neurons.

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The Electrostatic Origin of the Hydrogen-Bonding Cooperativity

The Electrostatic Origin of the Hydrogen-Bonding Cooperativi...

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中国化学会第九届全国量子化学学术会议

作者： Fan Jiang, Jun-Min Quan, and Yun-Dong Wu State Key laboratory of Molecular Dynamics and Stable Structures College of Chemistry, peking university, Beijing 100871 Department of Chemistry, The Hong Kong university of Science and Technology, Clear Water Bay, Kowloon, Hong Kong chemical Genetics laboratory, shenzhen graduate school, peking university, shenzhen 518000

＜正＞The cooperative interactions of several systems with and without hydrogen-bonding have been studied by density functional theory calculations. These systems include the chains of urea and formaldehyde, 4--hydroxybutenal, 1,2-dihydroxyethene/butenedial;ribbon structures of polyglycine, poly-β-alanine, and poly-aminoxy acids;and polyketone. These calculations indicate that a hydrogen bond chain does not necessarily cause a significant long-range cooperative interaction but through space electrostatic interaction/polarization is the main cause of cooperative interaction. In particular,

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